Molecular biology: principles of genome function
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford
Oxford University Press
2010
|
| Edition: | 1. publ. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XLI, 839 S. zahlr. Ill., graph. Darst. 28 cm |
| ISBN: | 9780199562053 0199562059 9780199562060 0199562067 |
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Record in the Search Index
| _version_ | 1804141152547373056 |
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| adam_text | Tät * t äk.
É
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itt.
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Abbreviations
xxxvii
1
Genomes and the flow of biological
001
information
Introduction
001
1.1
The roots of biology
002
1.2
The genome: a working blueprint for life
006
1.3
Bringing genes to life: gene expression
010
1.4
Cellular infrastructure and gene expression
014
1.5
Expression of the genome
017
1.6
Evolution of the genome and the tree of life
021
Summary
026
2
Biological molecules
028
Introduction
028
2.1
Atoms, molecules, and chemical bonds
029
2.2
Life in aqueous solution
033
2.3
Non-covalent interactions
035
2.4
Nucleotides and nucleic acids
039
2.5
The structure of
DNA 042
2.6
Chemical properties of
RNA
051
2.7
RNA
folding and structure
054
2.8
The
RNA
world and its role in the evolution of modern-day life
057
2.9
Fundamentals of protein structure
060
2.10
Protein folding
064
2.11
Protein folds
069
2.12
Sugars and carbohydrates
071
2.13
Lipids
075
2.14
Chemical modification in biological regulation
079
Summary
080
Further reading
082
3
The chemical basis of life
083
Introduction
083
3.1
Thermodynamic rules in biological systems
084
3.2
Binding equilibria and kinetics
091
3.3
Binding processes in biology
096
3.4
Enzyme catalysis
102
3.5
Enzyme kinetics
109
Summary
111
Further reading
113
XXX CONTENTS IN
FULL
4
Chromosome structure and function
114
Introduction
114
4.1
Organization of chromosomes
115
4.2
The cell cycle and chromosome dynamics
117
4.3
Packaging chromosomal
DNA 119
4.4
Variation in chromatin structure
126
4.5
Covalent modifications of histones
128
4.6
Nucleosome-remodeling complexes
133
4.7
DNAmethylation
135
4.8
Boundary elements separate chromatin domains
139
4.9
Elements required for chromosome function
142
4.10
The centromere
143
4.11
Thetelomere
147
4.12
Chromosome architecture in the nucleus
150
Summary
154
Further reading
155
5
The cell cycle
157
Introduction
157
5.1
Steps in the eukaryotic cell cycle
157
5.2
Cyclins and cyclin-dependent kinases
162
5.3
Regulation of Cdk activity
166
5.4
Cell cycle regulation by Cdks
168
5.5
Regulation of proteolysis by Cdks
170
5.6
Checkpoints: intrinsic pathways that can halt the cell cycle
176
5.7
Extrinsic regulators of cell cycle progression
180
5.8
The cell cycle and cancer
182
5.9
The bacterial cell cycle
185
Summary
190
Further reading
192
193
193
193
201
201
203
205
207
212
215
215
219
222
6 DNA
replication
Introduction
6.1
Overview of
DNA
replication
DNA
replication: core components
6.2
DNA polymerases:
structure and function
6.3
DNA
polymerases: fidelity and processivity
6.4
Specialized polymerases
6.5
DNA helicases:
unwinding of the double helix
6.6
The sliding clamp and clamp loader
DNA
replication: mechanism
6.7
Origins and initiation of
DNA
replication
6.8
Leading and lagging strand synthesis
6.9
The replication fork
CONTENTS IN
FULL
XXXI
6.10
Termination of
DNA
replication
226
6.11
The end-replication problem and telomerase
228
6.12
Chromatin replication
232
DNA
replication: regulation
234
6.13
Regulation of initiation of replication in
£
coli
234
6.14
Regulation of replication initiation in eukaryotes
236
Summary
240
Further reading
241
7
Chromosome segregation
243
Introduction
243
7.1
The stages of mitosis
243
7.2
Chromosome condensation and cohesion
245
7.3
The mitotic spindle
251
7.4
Prometaphase and metaphase
257
7.5 Anaphase: an
irreversible step in chromosome segregation
265
7.6
The completion of mitosis and cytokinesis
269
7.7
Meiosis: generating haploid gametes from diploid cells
271
7.8
Chromosome segregation in bacteria
280
Summary
286
Further reading
287
8
Transcription
288
Introduction
288
8.1
Overview of transcription
288
Transcription: core components
291
8.2
RNA polymerase
core enzyme
291
Transcription: mechanism
294
8.3
Promoter recognition in bacteria and eukaryotes
294
8.4
Initiation of transcription and transition to an elongating complex
301
8.5
Transcription elongation
309
8.6
Transcription termination
314
Transcription: regulation
316
8.7
Principles of transcription regulation
317
8.8
DNA-binding domains in transcriptional regulators
322
8.9
Mechanisms for regulating transcription in bacteria
327
8.10
Competition between cl and Cro and the fate of bacteriophage lambda
331
8.11
Mechanisms for modulating eukaryotic transcription
337
8.12
Combinatorial regulation of eukaryotic transcription
339
8.13
Signaling cascades and regulation of transcription
342
8.14
Regulation of elongation and termination by
RNA
and proteins
345
8.15
Transcriptional silencing
352
Summary
357
Further reading
359
XXXII
CONTENTS
IN FULL
9
RNA
processing
Introduction
9.1
Overview of
RNA
processing
9.2
tRNA and rRNA processing
9.3
tRNA and rRNA nucleotide modifications
9.4
mRNA capping and polyadenylation
9.5
RNA
splicing
9.6
Eukaryotic mRNA splicing by the spliceosome
9.7
Exon definition and alternative splicing
9.8
RNA
editing
9.9
Degradation of normal RNAs
9.10
Degradation of foreign and defective RNAs
9.11
RNA-binding domains in proteins
Summary
Further reading
10
Translation
Introduction
10.1
Overview of translation
Translation: core components
10.2
Transfer
RNA
and the genetic code
10.3
Aminoacyl-tRNA synthetases
10.4
Structure of the ribosome
Translation: mechanism
10.5
The translation cycle: the ribosome in action
10.6
Protein factors critical to the translation cycle
10.7
Translation initiation (mostly in bacteria)
10.8
Translation initiation in eukaryotes
10.9
Translation elongation: decoding,
peptide
bond formation,
and
translocation
10.10
Translation termination and reinitiation
Translation: regulation
10.11
Recoding: programmed stop codon read-through and frameshifting
10.12
Antibiotics that target the ribosome
10.13
Global regulation of initiation in bacteria and eukaryotes
10.14
Regulation of initiation via the
5
UTR
in bacteria and eukaryotes
10.15
Regulation of translation via the
3
UTR
in eukaryotes
10.16
Viral corruption of the translational machinery
Summary
Further reading
11
Protein modification and targeting
Introduction
11.1
Chaperone-assisted protein folding
11.2
Targeting of proteins throughout the cell
361
361
361
364
366
369
372
377
381
387
392
395
400
403
404
406
406
406
408
408
412
416
420
420
423
427
430
434
438
444
444
451
453
456
460
467
470
471
473
473
474
478
CONTENTS IN
FULL
XXXI
11
11.3
Post-translational cleavage of the polypeptide chain
482
11.4
Lipid
modification of proteins
487
11.5
Glycosylation of proteins
490
11.6
Protein phosphorylation, acetylation, and methylation
496
11.7
Protein oxidation, nitrosylation, and nitration
504
11.8
Ubiquitination and sumoylation of proteins
506
11.9
Protein degradation
513
Summary
516
Further reading
517
12
Cellular responses to
DNA
damage
519
Introduction
519
12.1
Types of
DNA
damage
520
12.2
Post-replication mismatch repair
523
12.3
Repair of
DNA
damage by direct reversal
526
12.4
Repair of
DNA
damage by base excision repair
527
12.5
Nucleotide excision repair of bulky lesions
528
12.6 Translesion DNA
synthesis
531
12.7
The
DNA
damage response
533
12.8
The
DNA
damage response in bacteria
537
12.9
The
DNA
damage response in eukaryotes
539
12.10 DNA
damage and cell death in mammalian cells
550
Summary
553
Further reading
554
13
Repair of
DNA
double-strand breaks
and homologous recombination
556
Introduction
556
13.1
An overview of
DNA
double-strand break repair
and homologous recombination
557
13.2
Double-strand break repair by non-homologous end joining
559
13.3
Homology-directed repair of double-strand breaks
562
13.4
Generation of single-stranded
DNA
by
helicases
and nucleases
564
13.5
The mechanism of
DNA
strand pairing and exchange
565
13.6
Gene conversion through homology-directed repair
571
13.7
Homologous recombination
576
13.8
Repair of damaged replication forks by homology-directed repair
589
13.9
Aberrant repair and recombination and chromosome rearrangements
590
Summary
596
Further reading
598
14
Mobile
DNA 599
Introduction
599
14.1
Transposable elements: overview
599
XXXIV
CONTENTS
IN RAJ.
14.2 DNA-only
transposons
609
14.3
Mechanism of
DNA-only cut-and-paste
transposition
reactions
610
14.4
Mechanism of DNA-only nick-and-paste transposition reactions
616
14.5
Cellular domestication of
a
DNA
cut-and-paste transposase
in adaptive immunity
620
14.6
Retrotransposons
624
14.7
LTR retrotransposons
625
14.8
Non-LTR retrotransposons
630
14.9
Control of transposition
638
14.10
Conservative site-specific recombination: overview
645
14.11
CSSR conversion of
DNA dimers
to monomers: single-protein systems
649
14.12
CSSR conversion of
DNA
dimers to monomers:
systems regulated by accessory proteins
653
14.13
CSSR systems that control gene expression
653
Summary
664
Further reading
665
15
Genomics and genetic variation
668
Introduction
668
15.1
Genome sequences and sequencing projects
669
15.2
Finding functions in a genome
672
15.3
Evolutionary forces
679
15.4
The evolving genome
681
15.5
Duplication and divergence of gene function
686
15.6
Sequence comparisons
690
15.7
Phenotypic variation
694
15.8
Exploring evolution through genomics: examples
from mammalian genomes
701
15.9
Human genetic diseases
706
Summary
710
Further reading
712
16
Tools and techniques in molecular biology
714
Introduction
714
16.1
Model organisms
715
16.2
Cultured cells and viruses
723
16.3
Cloning and amplification of
DNA
and
RNA
sequences
727
16.4
Genome manipulation
734
16.5
Detection of biological molecules
742
16.6
Separation and isolation of biological molecules
747
16.7
Identification of biological molecules
755
16.8
Detection of specific
DNA
sequences
760
16.9
Detection of specific
RNA
molecules
765
16.10
Detection of specific proteins
769
16.11
Detection of interactions between molecules
772
CONTENTS IN
FULL
XXXV
16.12
Imaging cells and molecules
16.13
Molecular structure determination
16.14
Obtaining and analyzing a complete genome sequence
16.15
Mapping human disease genes
Summary
Online resources for genomics and model organisms
778
784
788
797
804
806
Glossary
Index
807
827
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| publisher | Oxford University Press |
| record_format | marc |
| spelling | Molecular biology principles of genome function Nancy Craig ... 1. publ. Oxford Oxford University Press 2010 XLI, 839 S. zahlr. Ill., graph. Darst. 28 cm txt rdacontent n rdamedia nc rdacarrier molecular biology cabt Genome Genomes Molecular Biology Molecular biology Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf (DE-588)4123623-3 Lehrbuch gnd-content Molekulargenetik (DE-588)4039987-4 s DE-604 Molekularbiologie (DE-588)4039983-7 s 1\p DE-604 Craig, Nancy L. Sonstige oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018980603&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Molecular biology principles of genome function molecular biology cabt Genome Genomes Molecular Biology Molecular biology Molekulargenetik (DE-588)4039987-4 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4039983-7 (DE-588)4123623-3 |
| title | Molecular biology principles of genome function |
| title_auth | Molecular biology principles of genome function |
| title_exact_search | Molecular biology principles of genome function |
| title_full | Molecular biology principles of genome function Nancy Craig ... |
| title_fullStr | Molecular biology principles of genome function Nancy Craig ... |
| title_full_unstemmed | Molecular biology principles of genome function Nancy Craig ... |
| title_short | Molecular biology |
| title_sort | molecular biology principles of genome function |
| title_sub | principles of genome function |
| topic | molecular biology cabt Genome Genomes Molecular Biology Molecular biology Molekulargenetik (DE-588)4039987-4 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | molecular biology Genome Genomes Molecular Biology Molecular biology Molekulargenetik Molekularbiologie Lehrbuch |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018980603&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT craignancyl molecularbiologyprinciplesofgenomefunction |