Immunocytochemical methods and protocols (Humana Press, 2010)
Includes bibliographical references and index
Overview of antibodies for immunochemistry / Constance Oliver, Maria Célia Jamur -- Introduction to the purification of antibodies / Ana Cristina Grodzki, Elsa Berenstein -- Antibody purification: ammonium sulfate fractionation or gel filtration / Ana Cristina Grodzki, Elsa Berenstein -- Antibody purification: ion-exchange chromatography / Ana Cristina Grodzki, Elsa Berenstein -- Antibody purification: affinity chromatography--Protein A and Protein G Sepharose / Ana Cristina Grodzki, Elsa Berenstein -- Conjugation of fluorochromes to antibodies / Su-Yau Mao, J. Michael Mullins -- Biotinylation of antibodies / Su-Yau Mao -- Cell fixatives for immunostaining / Maria Célia Jamur, Constance Oliver -- Permeabilization of cell membranes / Maria Célia Jamur, Constance Oliver -- Preparation of frozen sections for analysis / Gary L. Bratthauer -- Processing of cytological specimens / Gary L. Bratthauer -- Processing of tissue culture cells / Gary L. Bratthauer --
Processing of tissue specimens / Gary L. Bratthauer -- Heat-induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections / Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, Mark Raffeld -- Fluorochromes: properties and characteristics / J. Michael Mullins -- Direct immunofluorescent labeling of cells / Maria Veronica Pástor -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescent visualization of macromolecules in Drosophila whole mounts / Ricardo Guelerman Ramos, Luciana Claudia Machado, Livia Maria Moda -- Overview of conventional fluorescence photomicrography / J. Michael Mullins -- Overview of confocal microscopy / William D. Swaim -- Overview of laser microbeam applications as related to antibody targeting / P. Scott Pine --
Immuno-laser capture microdissection of rat brain neurons for real time quantitative PCR / Denis G. Baskin, L. Scot Bastian -- Overview of antigen detection through enzymatic activity / Gary L. Bratthauer -- The Peroxidase-Antiperoxidase (PAP) method and other all-immunologic detection methods / Gary L. Bratthauer -- The Avidin-Biotin Complex (ABC) method and other Avidin-Biotin binding methods / Gary L. Bratthauer -- Avidin-Biotin labeling of cellular antigens in cryostat-sectioned tissue / Mark Raffeld, Elaine S. Jaffe -- Multiple antigen immunostaining procedures / Tibor Krenacs, Laszlo Krenacs, Mark Raffeld -- Immunoenzymatic quantitative analysis of antigens expressed on the cell Surface (cell-ELISA) / Elaine Vicente Lourenço, Maria-Cristina Roque-Barreira -- Use of immunogold with silver enhancement / Constance Oliver -- Overview of flow cytometry and fluorescent probes for flow cytometry / Robert E. Cunningham -- Tissue disaggregation / Robert E. Cunningham --
Indirect immunofluorescent labeling of viable cells / Robert E. Cunningham -- Indirect immunofluorescent labeling of fixed cells / Robert E. Cunningham -- Fluorescent labeling of DNA / Robert E. Cunningham -- Deparaffinization and processing of pathologic material / Robert E. Cunningham -- Fixation and embedding / Constance Oliver, Maria Célia Jamur -- Preparation of colloidal gold / Constance Oliver -- Conjugation of colloidal gold to proteins / Constance Oliver -- Colloidal gold/streptavidin methods / Constance Oliver -- Pre-embedding labeling methods / Constance Oliver -- Postembedding labeling methods / Constance Oliver -- The Clinical immunohistochemistry laboratory: regulations and troubleshooting guidelines / Patricia A. Fetsch, Andrea Abati