Single particle tracking and single molecule energy transfer:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH-Verl.
2010
|
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XVI, 343 S. Ill., graph. Darst. |
| ISBN: | 9783527322961 |
Internformat
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| 650 | 4 | |a Energy transfer | |
| 650 | 4 | |a Molecular Probe Techniques | |
| 650 | 4 | |a Nanotechnology | |
| 650 | 4 | |a Particle tracks (Nuclear physics) | |
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Datensatz im Suchindex
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CONTENTS PREFACE XI LIST OF CONTRIBUTORS XIII PART I SINGLE-PARTICLE
IMAGING AND TRACKING 1 1 THREE-DIMENSIONAL PARTICLE TRACKING IN A LASER
SCANNING FLUORESCENCE MICROSCOPE 3 VALERIA LEVI AND ENRICO GRATTON 1.1
INTRODUCTION 3 1.2 IMAGE-BASED SINGLE-PARTICLE TRACKING METHODS 5 1.3
ADVANCED FLUORESCENCE MICROSCOPY TECHNIQUES FOR SINGLE-PARTICLE TRACKING
7 1.4 TWO-PHOTON EXCITATION MICROSCOPY 9 1.5 3-D TRACKING IN IMAGE-BASED
SPT APPROACHES 10 1.6 3-D TRACKING IN LASER SCANNING MICROSCOPES 12 1.7
INSTRUMENTATION 14 1.8 BACKGROUND NOISE 15 1.9 SIMULTANEOUS TWO-PARTICLE
TRACKING 15 1.10 APPLICATION: CHROMATIN DYNAMICS IN INTERPHASE CELLS 16
1.11 CONCLUSIONS 18 REFERENCES 20 2 THE TRACKING OF INDIVIDUAL MOLECULES
IN CELLS AND TISSUES 25 LAURENT HOLTZER AND THOMAS SCHMIDT 2.1
INTRODUCTION 25 2.2 SINGLE-MOLECULE AND SINGLE-PARTICLE LOCALIZATION 26
2.3 POSITIONAL ACCURACY 30 2.4 TRACKING 32 2.5 TRAJECTORY ANALYSIS 33
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/994010605 DIGITALISIERT
DURCH VI I CONTENTS 3 MESSENGER RNA TRAFFICKING IN LIVING CELLS 43
ULRICH KUBITSCHECK, ROMAN VEITH,JOERG RITTER, AND JAN-PETER SIEBRASSE 3.1
INTRANUCLEAR STRUCTURE AND DYNAMICS 43 3.2 FCS AND FRAP STUDIES OF
NUCLEAR MRNP MOBILITY 44 3.3 SINGLE-PARTICLE TRACKING OF MRNA MOLECULES
44 3.3.1 THE AIM AND PURPOSE OF INTRANUCLEAR SINGLE MRNA TRACKING 44
3.3.2 SINGLE-PARTICLE TRACKING OF "DESIGNER" MRNPS 45 3.4
SINGLE-PARTICLE TRACKING OF SPECIFIC, NATIVE MRNPS 47 3.4.1 PROPERTIES
OF CHIRONOMUS TENTANS SALIVARY GLAND CELLS 47 3.4.2 MEASUREMENT OF
NUCLEAR VISCOSITY 49 3.4.3 MOBILITY MEASUREMENTS BY PHOTOBLEACHING 49
3.4.4 ANALYSIS OF MOBILITY BY SINGLE-PARTICLE TRACKING 50 3.4.5 TRACER
PARTICLES IN CELL NUCLEI 52 3.5 IN VIVO LABELING OF NATIVE BR2 MRNPS 52
3.5.1 MOBILITY ANALYSIS OF BR2 MRNPS 53 3.5.2 BR MRNPS DIFFUSE IN A
DISCONTINUOUS FASHION 56 3.5.3 VERIFICATION OF PARTICLE IDENTITY AND
HIGH MRNP MOBILITY 57 3.5.4 A DISCUSSION OF MRNP MOBILITY 59 CONTENTS
VII 4.7.2 TRACKING SINGLE MEMBRANE RECEPTOR MOLECULES 85 4.7.3
VISUALIZING INTERNALIZATION PATHWAYS 88 4.7.4 TRACKING INTRACELLULAR
MOTOR MOLECULES 89 4.7.5 FROM SINGLE MOLECULES TO POPULATIONS 90 4.8
CONCLUSIONS AND PERSPECTIVES 91 REFERENCES 92 PART II ENERGY TRANSFER ON
THE NANOSCALE 97 5 SINGLE-PAIR FRET: AN OVERVIEW WITH RECENT
APPLICATIONS AND FUTURE PERSPECTIVES 99 DON C. LAMB 5.1 INTRODUCTION 99
5.2 PRINCIPLES OF FRET 100 5.3 SPFRET IN SOLUTION 102 5.3.1 EXPERIMENTAL
CONSIDERATIONS 103 5.3.2 PROTEIN-FOLDING KINETICS 104 5.3.3
CHAPERON-ASSISTED PROTEIN FOLDING 106 5.3.4 PHOTON DISTRIBUTION ANALYSIS
(PDA) 109 5.4 SPFRET ON IMMOBILIZED MOLECULES 111 5.4.1 IMMOBILIZATION
METHODS 112 5.4.2 MECHANISM OF DNA UNWINDING BY THE NS3 HELICASE 115
5.4.3 DYNAMICS OF THE TBP-NC2-DNA COMPLEX 116 5.4.4 HIDDEN MARKOV
MODELING 119 5.5 FUTURE PROSPECTS 120 VILI I CONTENTS 7 UNRAVELING THE
DYNAMICS BRIDGING PROTEIN STRUCTURE AND FUNCTION ONE MOLECULE AT A TIME
163 JEFFERY A. HANSON, YAN-WEN TAN, AND HAW YANG 7.1 INTRODUCTION 163
7.1.1 ROUGH ENERGY LANDSCAPE 165 7.1.2 FUNCTIONAL ROLES OF
CONFORMATIONAL DYNAMICS 167 7.2 CONVERTING CHEMICAL ENERGY TO MECHANICAL
WORK: MOLECULAR MOTORS I68 7.2.1 F 0 F, ATP SYNTHASE 169 7.2.2 KINESIN
169 7.3 ALLOSTERY IN PROTEINS 173 7.3.1 SHIFT IN CONFORMATIONAL
POPULATION 173 7.3.2 LIGAND BINDING AND RECOGNITION: CALMODULIN 174 7.4
ENZYME CATALYSIS 177 7.4.1 S LOWLY-VARYING CATALYTIC RATE:
SINGLE-MOLECULE ENZYMOLOGY 177 7.4.2 DIRECT MEASUREMENT OF ENZYME
CONFORMATIONAL DYNAMICS 178 7.4.3 MECHANISTIC ROLES OF ENZYME
CONFORMATIONAL DYNAMICS: ADENYLATE KINASE 181 7.5 CONCLUSIONS 186
REFERENCES 186 8 QUANTITATIVE DISTANCE AND POSITION MEASUREMENT USING
SINGLE-MOLECULE FRET 191 JENS MICHAELIS 8. CONTENTS IX 9.2.2 PLASMOME
NANOPARTICLES AS CLASSICAL ATOMS 219 9.3 COUPLING OF TWO OSCILLATING
DIPOLES 221 9.3.1 TWO SINGLE MOLECULES 221 9.3.2 TWO PLASMONIC
NANOPARTICLES 225 9.4 A DIPOLE CLOSE TO A SURFACE 228 9.4.1 A MOLECULE
CLOSE TO A METALLIC SURFACE 228 9.4.2 A METALLIC NANOPARTICLE CLOSE TO A
DIELECTRIC SURFACE 230 9.4.3 A DIPOLE SENSES ITS NANOENVIRONMENT VIA THE
MODIFICATION OF ITS RESONANCE SPECTRUM 231 9.5 A SINGLE MOLECULE AND A
SINGLE NANOPARTICLE 232 9.5.1 A MOLECULE CLOSE TO A DIELECTRIC
NANOPARTICLE 233 9.5.2 A MOLECULE CLOSE TO A METALLIC NANOPARTICLE 235
9.6 MODIFICATION OF THE SPONTANEOUS EMISSION AND QUANTUM EFFICIENCY BY
NANOANTENNAE 238 9.7 CONCLUSIONS 240 REFERENCES 240 10 ENERGY TRANSFER
IN SINGLE CONJUGATED POLYMER CHAINS 243 MANFRED J. WALTER AND JOHN M.
LUPTON 10.1 INTRODUCTION 243 10.2 WHY SINGLE CHAIN SPECTROSCOPY? 245
10.3 EXPERIMENTAL APPROACH AND MATERIAL SYSTEMS 247 10.4 PHOTOPHYSICS OF
SINGLE CONJUGATED POLYMER CHAINS 251 X I CONTENTS 11 REACTIONS AT THE
SINGLE-MOLECULE LEVEL 283 MAARTEN B.J. ROEFFAERS, CERT DE CREMER, BERT
F. SELS, DIRK E. DE VOS, AND JOHAN HOFKENS 11.1 INTRODUCTION 281 11.2
BIOCATALYSIS AT THE SINGLE-MOLECULE LEVEL 283 11.2.1 KINETICS OF SINGLE
BIOCATALYSTS 282 11.2.1.1 SINGLE ENZYME STUDIES 284 11.2.1.2 INDIVIDUAL
ENZYMATIC TURNOVER STUDIES 286 11.2.2 UNRAVELING THE MODES OF ACTION OF
INDIVIDUAL ENZYMES 294 11.2.2.1 PHOSPHOLIPASE AL ON PHOSPHOLIPID
BILAYERS 294 11.3 CHEMOCATALYSIS AT THE SINGLE-MOLECULE LEVEL 295 11.3.1
SPATIAL RESOLUTION 296 11.3.2 SORPTION AND DIFFUSION STUDIES ON
CHEMOCATALYTIC MATERIALS 297 11.3.2.1 INTERCRYSTALLINE DIFFUSION 297
11.3.2.2 INTRACRYSTALLINE DIFFUSION AT THE INDIVIDUAL PARTICLE LEVEL 297
11.3.2.3 INTRACRYSTALLINE DIFFUSION AT THE SINGLE-MOLECULE LEVEL 299
11.3.3 CHEMICAL TRANSFORMATION AT THE SINGLE-MOLECULE LEVEL 299 11.3.4
CONCLUSIONS 304 REFERENCES 305 12 VISUALIZING SINGLE-MOLECULE DIFFUSION
IN NANOCHANNEL SYSTEMS 309 CHRISTOPHE JUNG AND CHRISTOPH BRAEUCHLE 12.1
INTRODUCTION 309 |
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| title | Single particle tracking and single molecule energy transfer |
| title_auth | Single particle tracking and single molecule energy transfer |
| title_exact_search | Single particle tracking and single molecule energy transfer |
| title_full | Single particle tracking and single molecule energy transfer ed. by Christoph Bräuchle ... |
| title_fullStr | Single particle tracking and single molecule energy transfer ed. by Christoph Bräuchle ... |
| title_full_unstemmed | Single particle tracking and single molecule energy transfer ed. by Christoph Bräuchle ... |
| title_short | Single particle tracking and single molecule energy transfer |
| title_sort | single particle tracking and single molecule energy transfer |
| topic | Energy Transfer Energy transfer Molecular Probe Techniques Nanotechnology Particle tracks (Nuclear physics) Freies Molekül (DE-588)4489205-6 gnd |
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