Cilia: [3] Model organisms and intraflagellar transport
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in cell biology
93 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 390 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780123813770 |
Internformat
MARC
| LEADER | 00000nam a2200000 cc4500 | ||
|---|---|---|---|
| 001 | BV035961600 | ||
| 003 | DE-604 | ||
| 005 | 20100421 | ||
| 007 | t | ||
| 008 | 100119s2009 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123813770 |9 978-0-12-381377-0 | ||
| 035 | |a (OCoLC)633168833 | ||
| 035 | |a (DE-599)BVBBV035961600 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-29T |a DE-703 | ||
| 084 | |a WC 5100 |0 (DE-625)148108:13423 |2 rvk | ||
| 245 | 1 | 0 | |a Cilia |n [3] |p Model organisms and intraflagellar transport |c ed. by Stephen M. King ... |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2009 | |
| 300 | |a XVI, 390 S., [8] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 93 | |
| 490 | 0 | |a Methods in cell biology |v ... | |
| 650 | 0 | 7 | |a Membrantransport |0 (DE-588)4038575-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Molekulargenetik |0 (DE-588)4039987-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zilie |0 (DE-588)4190826-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Zilie |0 (DE-588)4190826-0 |D s |
| 689 | 0 | 1 | |a Membrantransport |0 (DE-588)4038575-9 |D s |
| 689 | 0 | 2 | |a Molekulargenetik |0 (DE-588)4039987-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a King, Stephen M. |e Sonstige |0 (DE-588)141104457 |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV035961549 |g 3 |
| 830 | 0 | |a Methods in cell biology |v 93 |w (DE-604)BV002534878 |9 93 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018855756&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-018855756 | ||
Datensatz im Suchindex
| _version_ | 1804140980076544000 |
|---|---|
| adam_text | CONTENTS
Contributors
Preface
1.
Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila
Drashti Dave,
Dorota Wloga,
and
Jacek Gaerlig
I. Introduction
2
II. Designing the Targeting Fragment for Gene Disruption
5
III. Biolistic Transformation Protocol (Germline Gene Knockout)
6
IV. Construction of Gene Knockout Heterokaryons
10
V. Disruption of Multiple Genes in the Germline
14
VI. Somatic (Macronuclear) Gene Knockout
16
VII.
Phenotypic Rescue by Introduction of a Wild-type Gene into a
Knockout Strain
17
References
18
2.
Approaches for Functional Analysis of
Flagellar
Proteins in African Trypanosomes
Michael Oberholzer, Miguel A. Lopez,
Katherine S.
Rahton,
and Kent
L.
Hill
I. Introduction
22
II. Rationale
23
III. Methods
24
IV. Materials
48
V. Summary and Outlook
52
References
53
3.
Tools for Analyzing Intraflagellar Transport in Trypanosomes
Daria
JulkowsL· and Philippe
Bastin
I. Introduction
60
II. Methodology
63
III. Perspectives
76
References
78
Contents
4. Schmidtea
mediterranea: A
Model System
for Analysis of Motile Cilia
Pantelemón Rómpalas,
Ramila
S. Patel-King,
ana
Stephen
M.
King
I. Introduction
82
II. Materials and Methods
84
III. Discussion
94
IV. Summary
96
References
97
5.
Targeted Gene Silencing by
RNA
Interference in Chlamydomonas
Eun-Jeong Kim and
Heriberte Cerutti
I. Introduction
100
II. Materials and Methods
102
III. Results and Discussion
105
IV. Summary
108
References
109
Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas
Dennis Diener
I. Introduction
112
II. Methods for GFP Imaging of IFT Proteins
113
III. IFT Cargo
116
References
118
7.
Genetic and Phenotypic Analysis of
Flagellar
Assembly Mutants
in Chlamydomonas reinhardtii
Carlo Iomini, Jacob E. Till, and Susan K. Dutcher
I. Introduction
122
II. Isolation of
Aflagellate
Strains
123
III. Isolation of Conditional
Flagellar
Assembly (fla) Mutants
124
IV. Characterizing Intranageflar Transport
infla
Mutants
128
V. Genetic Characterization
132
VI. Characterization of Genetic Interactions
139
References
141
8.
Recording and Analyzing IFT in Chlamydomonas
Flagella
William
Dentier,
Kristýn
VanderWaal, and Mary
E
Porter
I. Introduction
146
II. Methods
146
III. Summary I54
References
155
Contents
vii
9. Total
Internal Reflection Fluorescence (TIRF) Microscopy
of
Chlamy
domónos
Flagella
Benjamin
D.
Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B.
Witman, and
Wallace
F. Marshau
I. Introduction
158
II.
Rationale:
A History of
Flagellar
Microscopy
in Chlamydomonas
159
III. Materials
and Methods: Technical Considerations of
Chlamydomonas TIRF
166
IV. Discussion: Future Prospects for Chlamydomonas TIRF
170
V. Summary
174
References
174
10.
Purification of IFT Particle Proteins and Preparation of
Recombinant
Proteins for Structural and Functional Analysis
Robert
H. Behal,
Ewelina Betleja, and Douglas G. Cole
I. Introduction
180
II. Isolating IFT Particle Proteins from Chlamydomonas
Flagella
181
III.
Recombinant
IFT Particle Proteins
191
IV. Summary and Concluding Remarks
194
References
195
11.
Studying Cilia in Zebrafish
Iain
Drummond
I. Introduction
198
II. Summary
215
References
215
12.
Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor
Sensory Cilia
Christine Insinna,
Katherine
Luby-Phelps, Brian A. Link, and Joseph C. Besharse
I. Introduction
220
II. Comparison of Photoreceptor Model Systems
222
III. Zebrafish as a Model for Analysis of Photoreceptor IFT
223
IV. Mutant Zebrafish
223
V. Antisense Morpholino Oligonucleotides
225
VI. Transient
Transgenesis
Using Photoreceptor-Specific Promoters
226
VII.
Materials and Methods
229
VIII.
Transmission Electron Microscopy
231
References
232
Contents
13.
Analysis of Intraflagellar Transport in
C. elegans
Sensory Cilia
Limin Hao, Seyda
Acar,
James Evans, Guangshuo On, and Jonathan M. Schoky
I. Introduction
236
II. Rationale
245
III. Methods
250
IV. Materials
259
V. Discussion
260
VI. Summary
262
References
262
14.
Functional Genomics of Intraflagellar Transport-Associated Proteins in
С
Peter
N.
Inglis, Oliver E. Blacque, and Michel R.
Leroux
I. Introduction
268
II. Identification of Novel IFT-Associated and Ciliary Proteins
279
III. Characterization of Candidate and Known IFT-Associated Proteins
281
IV. Conclusions
298
References
299
15.
Generating Conditional Mutants to Analyze Ciliary Functions:
The Use of Cre—Lox Technology to Disrupt Cilia in Specific Organs
Amber K. O Connor, Robert A. Kesterson, and Bradley K. Yoder
I. Introduction
306
II. The Design of a Conditional Targeted
Alíele
307
III.
ES
Clone Selection and Manipulation
313
IV. The Cre Line
315
V. The Reporter
316
VI. Practical Notes
319
VII.
Conditional
Alíeles
in Cilia Biology
320
VIII.
Methods
321
References
328
16.
Imaging Intraflagellar Transport in Mammalian Primary Cilia
Tatiana
Y.
Besschetnova,
Barnali Roy,
andjagesh V.
Shah
I. Introduction
332
II. Rationale
334
III. Methods for Generation and Characterization of IFT/GFP
Fusion Cell Lines
335
IV. Live Imaging of Mammalian IFT
339
V. Outlook
345
References
345
Contents
17.
Analysis of Hedgehog Signaling in Mouse Intraflagellar Transport Mutants
Hyuk W.
Ko,
Aimin Liu, and Jonathan T. Eggenschivikr
I. Introduction
348
II. Rationale
350
III. Methods
360
IV. Materials
364
V. Summary
366
References
366
Index
371
Volume in Series
383
|
| any_adam_object | 1 |
| author_GND | (DE-588)141104457 |
| building | Verbundindex |
| bvnumber | BV035961600 |
| classification_rvk | WC 5100 |
| ctrlnum | (OCoLC)633168833 (DE-599)BVBBV035961600 |
| discipline | Biologie |
| edition | 1. ed. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01770nam a2200433 cc4500</leader><controlfield tag="001">BV035961600</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20100421 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">100119s2009 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123813770</subfield><subfield code="9">978-0-12-381377-0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)633168833</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV035961600</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-703</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5100</subfield><subfield code="0">(DE-625)148108:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cilia</subfield><subfield code="n">[3]</subfield><subfield code="p">Model organisms and intraflagellar transport</subfield><subfield code="c">ed. by Stephen M. King ...</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Acad. Press</subfield><subfield code="c">2009</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XVI, 390 S., [8] Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in cell biology</subfield><subfield code="v">93</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in cell biology</subfield><subfield code="v">...</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Membrantransport</subfield><subfield code="0">(DE-588)4038575-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Molekulargenetik</subfield><subfield code="0">(DE-588)4039987-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zilie</subfield><subfield code="0">(DE-588)4190826-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Zilie</subfield><subfield code="0">(DE-588)4190826-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Membrantransport</subfield><subfield code="0">(DE-588)4038575-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Molekulargenetik</subfield><subfield code="0">(DE-588)4039987-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">King, Stephen M.</subfield><subfield code="e">Sonstige</subfield><subfield code="0">(DE-588)141104457</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="w">(DE-604)BV035961549</subfield><subfield code="g">3</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in cell biology</subfield><subfield code="v">93</subfield><subfield code="w">(DE-604)BV002534878</subfield><subfield code="9">93</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018855756&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-018855756</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV035961600 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:08:35Z |
| institution | BVB |
| isbn | 9780123813770 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018855756 |
| oclc_num | 633168833 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29T DE-703 |
| owner_facet | DE-355 DE-BY-UBR DE-29T DE-703 |
| physical | XVI, 390 S., [8] Bl. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Cilia [3] Model organisms and intraflagellar transport ed. by Stephen M. King ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2009 XVI, 390 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 93 Methods in cell biology ... Membrantransport (DE-588)4038575-9 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Zilie (DE-588)4190826-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zilie (DE-588)4190826-0 s Membrantransport (DE-588)4038575-9 s Molekulargenetik (DE-588)4039987-4 s DE-604 King, Stephen M. Sonstige (DE-588)141104457 oth (DE-604)BV035961549 3 Methods in cell biology 93 (DE-604)BV002534878 93 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018855756&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cilia Methods in cell biology Membrantransport (DE-588)4038575-9 gnd Molekulargenetik (DE-588)4039987-4 gnd Zilie (DE-588)4190826-0 gnd |
| subject_GND | (DE-588)4038575-9 (DE-588)4039987-4 (DE-588)4190826-0 (DE-588)4143413-4 |
| title | Cilia |
| title_auth | Cilia |
| title_exact_search | Cilia |
| title_full | Cilia [3] Model organisms and intraflagellar transport ed. by Stephen M. King ... |
| title_fullStr | Cilia [3] Model organisms and intraflagellar transport ed. by Stephen M. King ... |
| title_full_unstemmed | Cilia [3] Model organisms and intraflagellar transport ed. by Stephen M. King ... |
| title_short | Cilia |
| title_sort | cilia model organisms and intraflagellar transport |
| topic | Membrantransport (DE-588)4038575-9 gnd Molekulargenetik (DE-588)4039987-4 gnd Zilie (DE-588)4190826-0 gnd |
| topic_facet | Membrantransport Molekulargenetik Zilie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018855756&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035961549 (DE-604)BV002534878 |
| work_keys_str_mv | AT kingstephenm cilia3 |