Molecular diagnostics: promises and possibilities
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| Language: | English |
| Published: |
Dordrecht [u.a.]
Springer
2010
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Physical Description: | XX, 520 S. Ill., graph. Darst. 235 mm x 155 mm |
| ISBN: | 9789048132607 9789048132614 |
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| 100 | 1 | |a Debnath, Mousumi |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Molecular diagnostics |b promises and possibilities |c Mousumi Debnath ; Godavarthi B. K. S. Prasad ; Prakash S. Bisen |
| 264 | 1 | |a Dordrecht [u.a.] |b Springer |c 2010 | |
| 300 | |a XX, 520 S. |b Ill., graph. Darst. |c 235 mm x 155 mm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Molecular diagnosis | |
| 700 | 1 | |a Prasad, Godavarthi B. K. S. |e Verfasser |4 aut | |
| 700 | 1 | |a Bisen, Prakash S. |e Verfasser |4 aut | |
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| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018731646&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |3 Klappentext |
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| _version_ | 1804140849729110016 |
|---|---|
| adam_text | Contents
1
Introduction to Molecular Diagnostics
................ 1
1.1
Prologue
............................. 1
1.2
Clinical Diagnostics Entering a New Phase
........... 2
1.3
Concept of Molecular Diagnostics
............... 3
1.4
Molecular Diagnostic Technology and Health
Care Industries
......................... 4
1.5
Molecular Diagnostic Approaches
............... 6
1.5.1
Nucleic Acid Test Systems
.............. 7
1.5.2
Gene-Based Diagnostics
................ 8
1.6
Conclusion
........................... 10
References
................................ 10
2
Omics Technology
........................... 11
2.1
Prologue
............................. 11
2.2
Concept of Omics
........................ 12
2.3
Genome and Genomics
..................... 13
2.4
Transcriptome
and Transcriptomics
............... 15
2.5
Proteome and Proteomics
.................... 17
2.6
Metabolome and Metabolomics
................. 21
2.7
Integration of Omics
...................... 23
2.8
Conclusion
........................... 27
References
................................ 29
3
Recombinant
DNA
Pharmaceuticals
................. 33
3.1
Prologue
............................. 34
3.2
Concept
............................. 34
3.3
Tools of
Recombinant
DNA
Technology
............ 35
3.3.1
Common Enzymes Used in
Recombinant
DNA
Technology
................... 36
3.3.2
Vectors
......................... 38
3.3.3
Hosts
.......................... 38
3.3.4 DNA
Libraries
..................... 42
3.3.5
Library Screening
................... 43
3.3.6
Polymerase Chain Reaction
.............. 44
Contents
3.3.7
Analysis of Cloned
DNA
Sequences
......... 46
3.3.8
Expression of Cloned Genes
.............. 46
3.4
Therapeutic Applications of
Recombinant
DNA
Derived Pharmaceuticals
.................... 47
3.4.1
Recombinant
Therapeutic Proteins
.......... 47
3.4.2
Recombinant
DNA
Technology and Medicine
.... 49
3.5
Conclusion
........................... 51
References
................................ 52
Aptamers: In Vitro
DNA
Selection
.................. 55
4.1
Prologue
............................. 55
4.2
Concept
............................. 56
4.3
Selection of Aptamers
...................... 57
4.4
Applications of
DNA
and
RNA
Aptamers
........... 60
4.4.1
Aptamers for Detection of Pathogens
......... 61
4.4.2
Aptamers for Therapy
................. 62
4.4.3
Aptamers to Detect Change in Gene Expression
... 63
4.4.4
Aptamers as Diagnostics and Therapeutics
...... 64
4.4.5
Aptamers as Radiopharmaceutical Tools
....... 64
4.5
Conclusion
........................... 65
References
................................ 66
Reporter Gene
............................. 71
5.1
Prologue
............................. 71
5.2
Concept
............................. 71
5.3
Reporter Gene Assay
...................... 74
5.3.1
Luc Reporter Gene System
.............. 75
5.3.2
Rue Reporter Gene System
.............. 76
5.3.3
Green Fluorescent Protein (GFP) Reporter
Gene System
...................... 76
5.3.4
Human Growth Hormone (hGH) Reporter
Gene System
...................... 77
5.3.5
Beta-Galactosidase Reporter Gene System
...... 77
5.3.6
CAT Reporter Gene System
.............. 78
5.3.7
LacZ Reporter Gene System
.............. 78
5.3.8 GUS
Reporter Gene System
.............. 79
5.4
Transformation and Transfection Assays
............ 79
5.5
Gene Expression Assays
.................... 80
5.6
Promoter Assays
........................ 80
5.7
Role of Reporter Gene Assay in Molecular Diagnostics
.... 80
5.8
Conclusion
........................... 82
References
................................ 82
Impact of HGP on Molecular Diagnostics
.............. 85
6.1
Prologue
............................. 85
6.2
Origin of the Human Genome Project
............. 85
Contents xi
6.3 Goals
and Issues of HGP
.................... 86
6.4
The Human Genome and Genetic Variation
.......... 88
6.5
Human Genome Project and Health Services
.......... 89
6.6
Single Gene Disease
...................... 89
6.7
New and Individualised Drug Treatments
........... 91
6.8
The Achievements and Challenges of the HGP
......... 91
6.9
Information Transfer
...................... 92
6.10
Impact of the Human Genome Project
on
Epidemiologie
Research
................... 93
6.11
Impact of the Human Genome Project on Our Genomic Makeup
94
6.12
Conclusion
........................... 95
References
................................ 96
7
Molecular Diagnosis in the Post Genomic
and Proteomic Era
........................... 97
7.1
Prologue
............................. 97
7.2
The Genomic Era
........................ 97
7.3
Concept
............................. 98
7.4
The Post Genomic Era
..................... 101
7.5
Advantages of Combining Multiple Types of Data
....... 102
7.6
Biomedical
Research in the Postgenomic Era
......... 104
7.7
Conclusion
.........................., 106
References
................................ 107
8
Ethics, Patents and Regulations
....................
Ill
8.1
Prologue
.............................
Ill
8.2
Concept of Ethical, Legal, and Social Issues
..........
Ill
8.3
Ethical and Social Issues in Diagnostic Molecular Genetics
. . 112
8.4
Confronting the Ethical, Legal and Social Issues
........ 114
8.5
Genes and Disease
....................... 115
8.6
The Existence of Genetic Information
............. 117
8.6.1
Genetic Treatment of Disease
............. 119
8.6.2
Genetic Testing
.................... 120
8.6.3
Discrimination
..................... 122
8.7
Impact of the Human Genome Project at the Interface
Between Patent and FDA Law
................. 123
8.8
Regulatory Process
....................... 125
8.9
Conclusion
........................... 126
References
................................ 127
9
Polymerase Chain Reaction
...................... 129
9.1
Prologue
............................. 129
9.2
The Concept of Polymerase Chain Reaction
.......... 130
9.3
PCR Optimisation
........................ 132
9.3.1
Magnesium Concentration
............... 132
9.3.2
Buffer Concentration
................. 132
xii Contents
9.3.3 Enzyme
Concentration
................. 133
9.3.4 PCR Primer Design.................. 133
9.3.5 Template
Quality
.................... 133
9.3.6 Template
Quantity
................... 134
9.3.7
Cycling
Parameter................... 134
9.3.8 PCR
Enhancers and
Additives............. 135
9.3.9
Nucleic Acid Cross-Contamination..........
135
9.4
Advances in the PCR Technique
................ 136
9.4.1
RT-PCR
........................ 137
9.4.2
Hot Start PCR
.................... 137
9.4.3
Long Range PCR
................... 138
9.4.4
Inverse PCR
...................... 138
9.4.5
Anchored PCR
..................... 138
9.4.6
Nested Primer PCR
.................. 139
9.4.7
Colony PCR
...................... 139
9.4.8
Quantitative PCR
................... 139
9.4.9
Real-Time PCR
.................... 140
9.4.10
Rapid Amplification ofcDNA Ends (RACE)
..... 141
9.4.11
AFLP
.......................... 141
9.4.12
In Situ PCR
...................... 142
9.5
Cloning PCR Products
..................... 142
9.5.1
T
-А
Cloning Strategy
................. 142
9.5.2
Incorporation of Restriction Sites in Primers
..... 143
9.6
PCR as a Diagnostic Tool
.................... 143
9.7
Conclusion
........................... 147
References
................................ 148
10
In Situ Hybridization
.......................... 153
10.1
Prologue
............................. 153
10.2
The Concept of In Situ Hybridization
.............. 154
10.2.1
The Process
...................... 154
10.3
Disadavantages of Radioactive Probes
............. 156
10.4
Solving the Problem: Advent of FISH Technique
....... 156
10.4.1
Fluorescence In Situ Hybridization
.......... 157
10.4.2
FISH in Action
..................... 159
10.5
Applications of FISH as a Diagnostic Tool for Research
. ... 160
10.5.1
To Analyze the Onset of Specific Gene Expression
. . 161
10.5.2
Analysis of the Chromosome Structure
........ 161
10.5.3
Localisation of
RNA
Transcripts
........... 162
10.5.4
FISH as a Molecular Cytogenetic Technique
to Understand Diseases
................ 162
10.5.5
ИЅН
for Detection of Pathogens
........... 165
10.6
Recent Advances of In Situ Hybridisation Technology
..... 165
10.7
Conclusion
........................... 166
References
................................ 167
Contents xiü
11 Immunoassay.............................. 171
11.1
Prologue.............................
171
11.2
Concept
............................. 172
11.3
Types of
Immunoassay..................... 172
11.4
Recent Advances in the Field of Immunodiagnostics
...... 174
11.5
Clinical Applications of Immunoassay
............. 175
11.6
Utilization and Interpretation of Immunological Tests
..... 177
11.7
Conclusion
........................... 178
References
................................ 178
12
Phage Display
.............................. 181
12.1
Prologue
............................. 181
12.2
Concept
............................. 181
12.3
Phage-Display Libraries as Populations of
Replicatile,
Mutable Chemicals
....................... 182
12.4
Practical Applications of Phage Display
............ 184
12.4.1
Target Receptors Used in Affinity Selection
...... 184
YLA
2
Epitope Mapping and Mimicking
........... 185
12.4.3
Identifying New Receptors and Natural Ligands
... 186
12.4.4
Drug Discovery
.................... 186
12.4.5
Epitope Discovery
-
A New Route to Vaccines
and Diagnostics
.................... 187
12.4.6
Selection of DNA-Binding Proteins
.......... 188
12.4.7
Landscape Libraries as a Source of New Materials
. . 189
12.4.8
Phage Display-Combinatorial Chemistry on
the Cheap
....................... 190
12.4.9
Cloning Allergens by Phage Display
......... 190
12.5
Conclusion
........................... 19
1
References
................................ 192
13
Microarray
............................... 193
13.1
Prologue
............................. 193
13.2
The Concept of Microarray
................... 195
13.3
Current Challenges of Microarrays
............... 198
13.3.1
Gene Discovery
.................... 199
13.3.2
Gene Expression Profiling
............... 200
13.3.3
Pharmacogenomics and Microarray
.......... 202
13.3.4
Molecular Diagnostic Research
............ 204
13.4
Conclusion
........................... 205
References
................................ 206
14 DNA
Biosensors
............................. 209
14.1
Prologue
............................. 209
14.2
The Concept of
DNA
Biosensor
................ 210
14.3
Applications of Biosensors
................... 211
14.4
Advantages of Biosensors
.................... 212
xiv Contents
14.5 Development
of
DNA
Hybridization Biosensor
........ 213
14.6 DNA
Biosensor for Molecular Detection of Pathogens
..... 217
14.7
Biosensors as Analytical Tools in the Food and Drink Industries
218
14.8
Potential of Biosensor for Environmental Monitoring
..... 220
14.9
Conclusions
&
Future Challenges
............... 221
References
................................ 223
15
Molecular Microbiological Testing
.................. 227
15.1
Prologue
............................. 227
15.2
Concept
............................. 228
15.3
Advent of Improved Diagnostics
................ 229
15.4
Traditional Microbial Typing
.................. 230
15.4.1 Biotyping ....................... 230
15.4.2
Antibiograms, Resistograms, and Bacteriocin Typing
231
15.4.3
Protein Analysis
.................... 231
15.4.4
Phage Analysis
..................... 231
15.4.5 Chromatographie
Analysis
............... 231
15.5
Nucleic Acid-Based Typing Systems
.............. 232
15.5.1
Plasmid Analysis
.................... 232
15.5.2
Restriction Enzyme Pattern
.............. 232
15.5.3
Ribotyping
....................... 232
15.5.4
Random Amplified Polymorphic
DNA (RAPD) ... 232
15.5.5
Nucleic Acid Probes
.................. 233
15.5.6
Polymerase Chain Reaction
.............. 233
15.6
Current Application of Molecular Diagnostics
......... 234
15.6.1
Clinical Microbiology
................. 234
15.6.2
Clinical Epidemiology and Infection Control
..... 237
15.7
Promise of Molecular Testing
.................. 238
15.8
Assay Validation-Analytic Sensitivity and Specificity
..... 239
15.9
Conclusion
........................... 241
References
................................ 241
16
Proteomic Technology
......................... 245
16.1
Prologue
............................. 245
16.2
Concept
............................. 246
16.3
Methods to Perform Proteomic Analysis
............ 247
16.4
Functional Proteomics
..................... 249
16.5
Implications of the Human Genome Project
.......... 249
16.6
Measurement Using a Proteomic Approach
.......... 251
16.7
Use of Mass Spectrometry in Proteomics
............ 252
16.8
Proteomics: From Basic Research to Diagnostic
Application
........................... 253
16.9
Role of In Vitro Diagnostics (IVD) in Health Care
....... 254
16.10
Goals of Proteomics
....................... 257
16.11
Conclusion
........................... 258
References
................................ 259
Contents xv
17 Nanomedicine.............................. 261
17.1
Prologue.............................
261
17.2
Concept
............................. 262
17.3 Nanotechnology in
Medicine
.................. 264
17.3.1
Use of
Quantum
Dots
................. 265
17.3.2
Cell Targeting
..................... 266
17.3.3
New Nanocarriers for Drug Delivery
......... 267
17.3.4
Gene Therapy
..................... 270
17.3.5
Molecular Medicine
.................. 272
17.3.6
Construction of Nanostructure Template
....... 273
17.3.7
Targeting Drugs to Cells
................ 274
17.4
Nanodiagnostics
......................... 276
17.5
Molecular Electronics
...................... 278
17.6
Nanotechnology Biosensor for Pathogen Detection
...... 280
17.7
Nanotechnology Composite Materials for Next
Generation
Biomedical
Applications
.............. 280
17.8
A Carbon Nanotube Biosensor
................. 281
17.9
Conclusion
&
Future Directions
................ 282
References
................................ 283
18
Biomarkers
............................... 287
18.1
Prologue
............................. 287
18.2
Concept of
Biomarkers
..................... 288
18.3
Biomarkers
on the Horizon
................... 289
18.3.1
DNA Biomarkers ...................
290
18.3.2
RNA Biomarkers
.................... 290
18.3.3
Protein Biomarkers
.................. 291
18.4
New Applications
for Established
Biomarkers
......... 294
18.4.1
Development Pathways................
295
18.4.2
Identification of B
iomarkers
.............. 295
18.4.3
Chronology
of Biomarker
Development
........ 296
18.4.4
Role of
Biomarkers
in Cancer Detection,
Diagnosis, and Prognosis
............... 297
18.4.5
Biomarkers
in Cancer Therapy
............ 299
18.4.6
Biomarkers
in Early Detection
............. 302
18.4.7
Biomarkers
in Risk Assessment
............ 302
18.5
Organization of a Program in Protein
Biomarker
Discovery
............................ 303
18.5.1
Cancer Site Teams
................... 304
18.5.2
Biomarker
Mines
................... 304
18.5.3
Informatics Platform
.................. 305
18.5.4
Reagents Core
..................... 305
18.5.5
Incorporating Technology Improvements
....... 306
18.6
Conclusion
........................... 307
References
................................ 307
xvi
Contents
19
Diagnosis and Monitoring of Infections
................ 309
19.1
Prologue
............................. 309
19.2
Concept of Diagnosis and Monitoring of Infections
...... 310
19.2.1
Detection and Identification of Pathogens
Without Target Amplification
............. 311
19.2.2
Nucleic Acid Amplification
.............. 312
19.2.3
Detecting Antimicrobial-Drug Resistance
....... 314
19.3
Molecular Diagnostics of Infections
.............. 316
19.3.1
Sexually Transmitted Infections
............ 316
19.3.2
Vector Borne Disease
................. 318
19.3.3
Viral Infections
..................... 318
19.3.4
Bacterial Infections
.................. 321
19.3.5
Fungal Infections
................... 323
19.3.6
Practical Applications of Molecular Methods
in the Clinical Microbiology Laboratory
....... 323
19.4
Conclusion
........................... 327
References
................................ 328
20
Diagnosis of Mutation and Genetic Disorders
............ 331
20.1
Prologue
............................. 331
20.2
Concept
............................. 332
20.3
Factors Regulating a Genetic Disease
.............. 333
20.4
Genetic Testing
......................... 334
20.4.1
Cytogenetic Testing
.................. 334
20.4.2
Biochemical Testing
.................. 335
20.4.3
Molecular Testing
................... 336
20.5
Current Status of Molecular Diagnosis of Some
Common Genetic Diseases
................... 336
20.5.1
Cystic Fibrosis
..................... 337
20.5.2
Duchenne Muscular Dystrophy
............ 338
20.5.3
HaemophiliaA
..................... 338
20.5.4
Haemophilia
В
..................... 338
20.5.5
Phenylketonuria
.................... 339
20.5.6
β
Thalassaemia
..................... 339
20.5.7
Wilson s Disease (Hepatolenticular Degeneration)
. . 339
20.5.8
α
Antitrypsin Deficiency
................ 340
20.5.9
Familial Hypercholesterolaemia and Other
Lipoprotein Disorders
................. 340
20.5.10
Huntington s Disease Gene
.............. 340
20.5.11
Multiple Endocrine
Neoplasia
............. 341
20.5.12
Factor V (Leiden) Mutation
.............. 342
20.5.13
Hemochromatosis Gene, HFE
............. 342
20.5.14
Colon Cancer Gene, APC
............... 343
20.6
Microarray Analysis for Detection of Complex
Pattern of Genes
......................... 343
Contents xvii
20.7
Conclusion
........................... 344
References
................................ 344
21
Diagnosis of Complex Diseases
.................... 347
21.1
Prologue
............................. 347
21.2
Concept
............................. 348
21.3
Background Information
.................... 349
21.4
Detecting New Metabolic Disease Pathways
.......... 350
21.5
Phenotypic Variations in Simplex Diseases
........... 350
21.6
The Genetic Background of Complex Disorders
........ 351
21.7
Dissecting Interactions Between Genes and Environment
... 351
21.7.1
Alzheimer Disease
................... 353
21.7.2
Cancer
......................... 356
21.7.3
Multiple Sclerosis
................... 360
21.7.4
Diabetes
........................ 363
21.7.5
Hemophilia
....................... 364
21.7.6
Obesity
......................... 367
21.7.7
Schizophrenia
..................... 370
21.8
Conclusion
........................... 374
References
................................ 375
22 Biochips................................. 383
22.1
Prologue
............................. 383
22.2
Concept
............................. 383
22.3
Design of
Biochip........................ 385
22.4
Applications of
Biochips.................... 385
22.4.1 Biochip
and Clinical Medicine
............ 386
22.4.2 Biochips
and Biosensors
............... 387
22.4.3 Biochip
and Pharmacogenetics
............ 387
22.4.4 Biochip
and
Biowarfare................ 388
22.4.5 Biochip
and Diagnostics
................ 388
22.4.6 Biochip
and Cancer
.................. 389
22.4.7 Biochip
Department of Defense
............ 389
22.5 Biochip
Market
......................... 390
22.6
Future of
Biochips........................ 390
References
................................ 391
23
Personalised Medicine
......................... 393
23.1
Prologue
............................. 393
23.2
Concept
............................. 394
23.3
Practice of Medicine in the Twenty-First Century
....... 396
23.4
Role of Personalized Medicine in Drug Discovery
....... 396
23.5
Molecular Diagnosis Will Determine Prognosis
and Therapy
........................... 399
23.6
Personalized Medicine and Genetic Markers
.......... 400
23.7
Challenges of Realizing the Promise of Personalized Medicine
401
Contents
23.8
Personalised Medicine and Pharmacogenomics
........ 403
23.9
Personalized Medicine and Diseases
.............. 406
23.10
Personalized Medicine and Diagnostics Industry
........ 406
23.11
Impact of the US Patent System on the Promise
of Personalized Medicine
.................... 408
23.12
Ethical Legal and Social Issues of Personalised Medicine
. . . 409
23.13
Conclusion
........................... 410
References
................................ 410
24
Biopharmaceutical Industry and Health Care
............ 413
24.1
Prologue
............................. 413
24.2
Concept
............................. 414
24.3
Biopharmaceutical Research
.................. 415
24.4
Opportunities in Healthcare
................... 416
24.5
Molecular Diagnostics and Health Care
............ 417
24.6
Global Context
......................... 418
24.7
Emerging Biopharmaceuticals
................. 420
24.8
Challenges of the Biopharmaceutical Industry
......... 423
References
................................ 424
25
Forensic Medicine
........................... 425
25.1
Prologue
............................. 425
25.2
Concept
............................. 426
25.3
Forensic Medicine and
DNA
Fingerprinting
.......... 428
25.4
Applications of
DNA Methylation
Markers in Forensic Medicine
431
25.5
Forensic Medicine and Anthropometry
............. 431
25.6
Clinical Forensic Medicine
................... 432
25.7
Conclusion
........................... 433
References
................................ 433
26
Pharmacogenomics
........................... 435
26.1
Prologue
............................. 435
26.2
Concept
............................. 436
26.3
Predicting Drug Response on Gene Variation
......... 437
26.4
Drug Development and Testing Benefit
from Pharmacogenomics
.................... 437
26.5
Applications and Benefits of Pharmacogenomics
........ 438
26.5.1
Better, Safer Drugs the First Time
........... 438
26.5.2
More Accurate Methods of Determining
Appropriate Drug Dosages
.............. 438
26.6
Benefits of Pharmacogenomic Testing
............. 439
26.6.1
Patient s Ability to Metabolize Drugs
......... 439
26.6.2
Age-Related Genetic Variations
............ 440
26.7
Recent Reports of Pharmacogenomics Use
........... 441
26.8
Interpreting Pharmacogenomic Tests
.............. 442
26.9
Barriers to Pharmacogenomics Progress
............ 443
Contents xix
26.10
The Promise of Pharmacogenomics
............... 444
26.11
Impact of Drug Discovery, Development,
and Marketing Affected by Pharmacogenomics
........ 446
26.12
Pharmacogenomics Initiatives
................. 448
26.13
Pharmacogenomics and the Pharmaceutical Industry
...... 448
26.14
Applied Pharmacogenetics
................... 449
26.15
Ethical Concerns
........................ 450
26.16
Molecular Diagnostic Methods for Optimizing Drug Therapy
. 451
26.17
Challenges for the Future
.................... 452
References
................................ 453
27
Gene Technology in Forensic Sciences
................ 455
27.1
Prologue
............................. 455
27.2
Concept
............................. 456
27.3 DNA
Typing and Genetic Markers
............... 456
27.4 DNA
Based Methods for Identification of Individuals
..... 459
27.4.1
Non-PCR Based Methods
............... 459
27.4.2
PCR Based Methods
.................. 460
27.5
Ethical, Legal, and Social Concerns About
DNA Databanking 463
27.6
Future of
DNA
Typing Systems
................. 463
References
................................ 464
28
Food Diagnostics
............................ 467
28.1
Prologue
............................. 467
28.2
Concept
............................. 468
28.3
Tools of Molecular Diagnostics to Assess Food Quality
.... 469
28.4
Food Allergy
.......................... 470
28.5
Food-Borne Disease
....................... 470
28.6
Commercial Rapid Food-Safety Diagnostics
.......... 471
28.7
Methods for Analysis of Food
................. 472
28.7.1
Rapid Methods
..................... 473
28.8
Future Technologies
....................... 476
28.8.1
Microarrays
...................... 476
28.8.2
Sensor Technology
................... 477
28.8.3
Flow Cytometry
.................... 478
28.8.4
Bacteriophage-Based Techniques
........... 478
28.8.5
Riboprinting and Pulse-Field Gel Electrophoresis
. . 479
28.8.6
Applications and Limitations of Rapid Methods
. . . 479
28.9
Business Outlook of Food Diagnostics
............. 479
28.10
Conciusion
........................... 480
References
................................ 481
29
Rapid Diagnostic Methods for
Biowarfare.............. 483
29.1
Prologue
............................. 483
29.2
Concept
............................. 484
29.3
Characteristics of Biological Weapons
............. 485
xx
Contents
29.3.1
Anthrax
........................ 487
29.3.2
Plague
......................... 487
29.3.3
Tularemia .......................
487
29.3.4
Melioidosis
....................... 488
29.3.5
Viral Hemorrhagic Fevers
............... 488
29.3.6
Other Viral Fevers
................... 488
29.3.7
Trichothecene Mycotoxins
............... 488
29.3.8
Aflatoxin
........................ 489
29.4
Biodefense
............................ 489
29.4.1
Viral Agents
-
Poxviridae
............... 490
29.4.2
Botulinai
Toxins
.................... 490
29.4.3
Mycotoxins
...................... 491
29.5
Combating Detection of
Biowarfare
Agents
.......... 491
29.5.1
Prophylatic
&
Therapeutic Approaches
........ 491
29.5.2
Detection Methods
................... 495
29.6
Impact of Biological Weapons
................. 495
29.7
Tools for Self Defence Against Bioweapons
.......... 496
29.8
Genetic Engineering and Biological Warfare
.......... 497
29.9
Impact of Genomics and Genetic Technology
......... 499
29.10
Conclusion
........................... 500
References
................................ 500
30
Segments of Molecular Diagnostics
-
Market Place
......... 503
30.1
Prologue
............................. 504
30.2
Concept
............................. 504
30.3
Market Drivers
......................... 506
30.4
Trends in Infectious Diseases Testing Market
......... 507
30.5
Trends in Cancer Diagnostic Testing World Markets
...... 507
30.6
Trends in Cardiac Marker Diagnostic Testing Markets
..... 508
30.7
Point of Care Diagnostic Testing World Markets
........ 508
30.8
Next Generation Molecular Diagnostics
............ 509
30.9
The Rise of Companion Diagnostics
.............. 510
30.10
Market Considerations and Forecasts
.............. 510
References
................................ 513
Index
..................................... 515
Molecular
diagnostics
has become an important part of the clinical laboratory,
with tests and methods to identify a disease and understand the predisposition
for a disease by analyzing
DNA
or
RNA.
Molecular diagnostics offers a growth
opportunity in utilizing molecular tools to precisely target therapeutics and
the development of biochips, micro fluidics technology and nanotechnology
extends the limits of detection.
Molecular diagnostics can provide gene profile based personalized therapeutic
approaches, and therapy based on this molecular diagnostic information
delivers effective treatment with the least
toxicity, bi
the future, the scope of
molecular diagnostics in molecular medicine could be expanded well beyond
current nucleic acid testing, and it will play an important role in medicine,
public health, pharmaceutics, forensics and drug discovery.
Molecular Diagnostics: Promises and Possibilities covers:
•
The identification of viable technology drivers through a comprehensive look
at platform technologies for molecular diagnostics, including probe-based
nucleic acid assays, microarrays and sequencing
•
The most important molecular diagnostics tests: predictive, screening,
prognostic, monitoring, pharmacogenomic and theranostic from their basic
principles to their applications
•
The discovery of feasible market opportunities by identifying high-growth
applications in different clinical diagnostic areas and by focusing on expanding
markets, such as communicable diseases, cardiology and oncology
•
Global industry development through an in-depth analysis of the major world
markets for molecular diagnostics, including growth forecasts.
This much needed book is written for students,
scientists and professionals working in the field
of molecular diagnostics, as well as pamologists,
medical microbiologists, pharmaceutical scientists,
agricultural scientists and veterinary doctors. No
other book currently available provides such a
complete picture of the developments
m
the field of
molecular diagnostics.
|
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| author | Debnath, Mousumi Prasad, Godavarthi B. K. S. Bisen, Prakash S. |
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| author_role | aut aut aut |
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| dewey-ones | 616 - Diseases |
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| discipline | Biologie Medizin |
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| id | DE-604.BV035873913 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:06:31Z |
| institution | BVB |
| isbn | 9789048132607 9789048132614 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018731646 |
| oclc_num | 618950625 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | XX, 520 S. Ill., graph. Darst. 235 mm x 155 mm |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Springer |
| record_format | marc |
| spelling | Debnath, Mousumi Verfasser aut Molecular diagnostics promises and possibilities Mousumi Debnath ; Godavarthi B. K. S. Prasad ; Prakash S. Bisen Dordrecht [u.a.] Springer 2010 XX, 520 S. Ill., graph. Darst. 235 mm x 155 mm txt rdacontent n rdamedia nc rdacarrier Molecular diagnosis Prasad, Godavarthi B. K. S. Verfasser aut Bisen, Prakash S. Verfasser aut Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018731646&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018731646&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Debnath, Mousumi Prasad, Godavarthi B. K. S. Bisen, Prakash S. Molecular diagnostics promises and possibilities Molecular diagnosis |
| title | Molecular diagnostics promises and possibilities |
| title_auth | Molecular diagnostics promises and possibilities |
| title_exact_search | Molecular diagnostics promises and possibilities |
| title_full | Molecular diagnostics promises and possibilities Mousumi Debnath ; Godavarthi B. K. S. Prasad ; Prakash S. Bisen |
| title_fullStr | Molecular diagnostics promises and possibilities Mousumi Debnath ; Godavarthi B. K. S. Prasad ; Prakash S. Bisen |
| title_full_unstemmed | Molecular diagnostics promises and possibilities Mousumi Debnath ; Godavarthi B. K. S. Prasad ; Prakash S. Bisen |
| title_short | Molecular diagnostics |
| title_sort | molecular diagnostics promises and possibilities |
| title_sub | promises and possibilities |
| topic | Molecular diagnosis |
| topic_facet | Molecular diagnosis |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018731646&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018731646&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
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