Plant cell culture: essential methods
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley-Blackwell
2010
|
| Ausgabe: | 1. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 341 S. Ill., graph. Darst. |
| ISBN: | 9780470686485 |
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| 245 | 1 | 0 | |a Plant cell culture |b essential methods |c Michael R. Davey and Paul Anthony, [ed.] |
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| 264 | 1 | |a Chichester [u.a.] |b Wiley-Blackwell |c 2010 | |
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Datensatz im Suchindex
| _version_ | 1804140832037535744 |
|---|---|
| adam_text | Contents
Preface
xi
Contributors
xiii
1
Plant
Micropropagation
1
Ivan Iliev,
Alena Gajdošová, Gabríela Libiaková,
Shrì
Mohan
Jain
1.1
Introduction
1
1.2
Methods and approaches
2
1.2.1
Expiants
and their surface disinfection
2
1.2.2
Culture media and their preparation
4
1.2.3
Stages of
micropropagation
6
1.2.4
Techniques of
micropropagation
7
1.3
Troubleshooting
19
References
20
2
Thin Cell Layers: The Technique
25
Jaime
A. Teixeira
da Silva
and Michio Tanaka
2.1
Introduction
25
2.2
Methods and approaches
26
2.2.1
TCL
26
2.2.2
Choice of material: Cymbidium hybrid
26
2.3
Troubleshooting
35
2.3.1
General comments
35
References
36
3
Plant Regeneration
-
Somatic
Embryogenesis 39
Kim E. Nolan, Ray J. Rose
3.1
Introduction
39
3.2
Methods and approaches
40
3.2.1
Selection of the
cultivar
and type of
expiant
40
3.2.2
Culture media
41
3.2.3
Preparation of culture media
44
3.2.4
Sterilization of tissues and sterile technique
48
3.2.5
Culture and growth of tissue
51
vi
CONTENTS
3.2.6
Culture
and induction of somatic embryos
52
3.2.7
Embryo development
52
3.2.8
Transfer to soil
-
the final stage of regeneration
56
3.3
Troubleshooting
57
References
57
4
Haploid Plants
61
Sant
S.
Bhojwani and Prem
К.
Dantu
4.1
Introduction
61
4.2
Methods and approaches
62
4.2.1
Androgenesis
62
4.2.2
Diploidization
67
4.3
Troubleshooting
74
References
75
5
Embryo Rescue
79
Traud
Winkelmann, Antje Doil,
Sandra
Reinhardt
and
Aloma
Ewald
5.1
Introduction
79
5.2
Methods and approaches
80
5.2.1
Identification of the time and type of barrier in hybridization
80
5.2.2
Isolation of plant material after fertilization
81
5.2.3
Culture conditions and media
82
5.2.4
Confirmation of
hybridity
and ploidy
83
5.2.5
Conditions for regeneration of embryos to plants
86
5.3
Troubleshooting
93
References
93
6
In vitro Flowering and Seed Set: Acceleration of Generation
Cycles
97
Sergio J. Ochatt and Rajbir
5.
Sangwan
6.1
Introduction
97
6.2
Methods and approaches
98
6.2.1
Protein legumes
[7] 98
6.2.2
Arabidopsis thaliana
[13] 105
6.3
Troubleshooting
108
References
109
7
Induced Mutagenesis in Plants Using Physical and Chemical
Agents 111
Chikelu Mba, Rownak Afza, Souleymane
Bado
and Shri Mohan Jain
7.1
Introduction 111
7.2
Methods and approaches
112
7.2.1
Determination of the optimal doses of
mutagens
for inducing
mutations
112
7.3
Troubleshooting
126
7.3.1
Factors influencing the outcome of mutagenesis using chemical
mutagens
126
CONTENTS
vii
7.3.2
Factors influencing the outcome of mutagenesis using physical
mutagens
128
7.3.3
Facts about induced mutations
129
References
129
8
Cryopreservation of Plant Germplasm
131
E.R. Joachim Keller and
Angelika
Sen
и
la
8.1
Introduction
131
8.2
Methods and approaches
132
8.2.1
Main principles
132
8.2.2
Slow (two-step) freezing
134
8.2.3
Vitrification
134
8.2.4
Encapsulation-dehydration
135
8.2.5
DMSO droplet freezing
135
8.2.6
Combined methods
136
8.2.7
Freezing of cold-hardened buds
136
8.2.8
Freezing of orthodox seeds
136
8.2.9
Freezing of pollen and spores
137
8.3
Troubleshooting
149
References
150
9
Plant Protoplasts: Isolation, Culture and Plant Regeneration
153
Michael R. Davey, Paul Anthony, Deval
Patel
and
3.
Brian Power
9.1
Introduction
153
9.2
Methods and approaches
154
9.2.1
Protoplast isolation
154
9.2.2
Protoplast culture
156
9.3
Troubleshooting
170
References
171
10
Protoplast Fusion Technology
-
Somatic Hybridization and
Cybridization
175
Jude
W.
Grosser,
Milica Ćalović
and Eliezer
S. Louzada
10.1
Introduction
175
10.2
General applications of somatic hybridization
176
10.3
Methods and approaches
179
10.4
Troubleshooting
195
References
196
11
Genetic Transformation
-
Agrobacterium
199
Ian S. Curtis
11.1
Introduction
199
11.2
Methods and approaches
200
11.2.1
Agrobacterium as a natural genetic engineer
200
11.2.2
Vector systems for transformation
201
11.2.3
Inoculation procedures
202
viii
CONTENTS
11.3
Troubleshooting
213
References
214
12
Genetic Transformation
-
Biolistics
217
Fredy
Altpeter
and Sukhpreet Sandhu
12.1
Introduction
217
12.2
Methods and approaches
218
12.2.1
Biolistic technology
218
12.2.2
Optimization of gene delivery parameters
219
12.2.3
Target tissues
220
12.2.4
Reporter gene assays
230
12.2.5
Selection and plant regeneration
231
12.3
Troubleshooting
237
References
237
13
Plastici
Transformation
241
Bridget V. Hogg, Cilia L.C. Lelivelt, Aisling Dunne,
Кіт
-Hong
Nguyen
and Jacqueline M. Nugent
13.1
Introduction
241
13.2
Methods and approaches
243
13.2.1
Principles of plastid transformation
243
13.2.2
Biolistic-mediated plastid transformation
244
13.2.3
PEG-mediated plastid transformation
250
13.2.4
Identification and characterization of transplastomic plants
254
13.3
Troubleshooting
257
13.3.1
Biolistic-mediated transformation
257
13.3.2
PEG-mediated transformation
258
References
258
14
Molecular Characterization of Genetically Manipulated Plants
261
Cristiano
Lacorte, Giovanni Vianna, Francisco J.L
Aragão
and
Ebbio
Ĺ
Rech
14.1
Introduction
261
14.2
Methods and approaches
262
14.2.1
Plant
DNA
extraction
263
14.2.2
Polymerase chain reaction
266
14.2.3
Southern blot technique
268
14.2.4
Analysis of the integration site: inverse PCR (iPCR) and thermal
asymmetric interlaced PCR (Tail-PCR)
272
14.3
Troubleshooting
278
References
279
15
Bioreactors
281
Spiridon Kintzios
15.1
Introduction
281
15.2
Methods and approaches
283
CONTENTS ix
15.2.1 Medium
scale disposable or semidisposable airlift reactors
283
15.2.2
The RITA temporary immersion reactor
284
15.2.3
The LifeReactor
286
15.2.4
Immobilized cell bioreactors
289
15.2.5
Mini-bioreactors
289
15.3
Troubleshooting
292
References
294
16
Secondary Products
297
Kexuan Tang, Lei Zhang, Junfeng Chen, Ying Xiao, Wansheng Chen
and Xiaofen Sun
16.1
Introduction
297
16.2
Methods and approaches
298
16.2.1
Plant cell cultures
298
16.2.2
Scale-up and regulation of secondary metabolite production
303
16.2.3
Detection of secondary products
310
16.3
Troubleshooting
313
References 31A
17
Plant Cell Culture
-
Present and Future
317
Jim M. Dunwell
17.1
Introduction
317
17.2
Micropropagation
317
17.3 Embryogenesis 318
17.3.1
Background
318
17.3.2
Commercial exploitation of somatic embryos
318
17.3.3
Molecular aspects of somatic embryogenesis
318
17.3.4
Microspore
derived embryos
319
17.4
Haploid methodology
319
17.4.1
Haploids and their exploitation
319
17.4.2
Induction of haploid plants
320
17.4.3
Molecular aspects of haploid induction from
microspores
320
17.4.4 Ab
initio zygotic-like embryogenesis from
microspores
321
17.5
Somadonal variation
321
17.6
Transgenic methods
322
17.6.1
Background
322
17.6.2
Regeneration and transformation techniques
322
17.6.3
Chloroplast
transformation
322
17.6.4
Biopharming
323
17.7
Protoplasts and somatic hybridization
323
17.8
Bioreactors
323
17.8.1
Production of plant products
323
17.8.2
Production of Pharmaceuticals
323
17.8.3
Production of food ingredients
324
17.8.4
Production of cosmetics
324
17.8.5
Analytical methodology
324
χ
CONTENTS
17.9 Cryopreservation 324
17.10
Intellectual property and commercialization
324
17.10.1
Background
324
17.10.2
Sources of patent and other relevant information
325
17.11
Conclusion
325
References
325
Index
333
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| spelling | Plant cell culture essential methods Michael R. Davey and Paul Anthony, [ed.] 1. ed. Chichester [u.a.] Wiley-Blackwell 2010 XVI, 341 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Plant cell culture Plant tissue culture Zellkultur (DE-588)4067547-6 gnd rswk-swf Gewebekultur (DE-588)4157245-2 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzenzelle (DE-588)4115551-8 s Zellkultur (DE-588)4067547-6 s DE-604 Pflanzen (DE-588)4045539-7 s Gewebekultur (DE-588)4157245-2 s b DE-604 Davey, Michael R. 1944- Sonstige (DE-588)140962840 oth Anthony, Paul Sonstige oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018713828&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Plant cell culture essential methods Plant cell culture Plant tissue culture Zellkultur (DE-588)4067547-6 gnd Gewebekultur (DE-588)4157245-2 gnd Pflanzenzelle (DE-588)4115551-8 gnd Pflanzen (DE-588)4045539-7 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4157245-2 (DE-588)4115551-8 (DE-588)4045539-7 (DE-588)4143413-4 |
| title | Plant cell culture essential methods |
| title_auth | Plant cell culture essential methods |
| title_exact_search | Plant cell culture essential methods |
| title_full | Plant cell culture essential methods Michael R. Davey and Paul Anthony, [ed.] |
| title_fullStr | Plant cell culture essential methods Michael R. Davey and Paul Anthony, [ed.] |
| title_full_unstemmed | Plant cell culture essential methods Michael R. Davey and Paul Anthony, [ed.] |
| title_short | Plant cell culture |
| title_sort | plant cell culture essential methods |
| title_sub | essential methods |
| topic | Plant cell culture Plant tissue culture Zellkultur (DE-588)4067547-6 gnd Gewebekultur (DE-588)4157245-2 gnd Pflanzenzelle (DE-588)4115551-8 gnd Pflanzen (DE-588)4045539-7 gnd |
| topic_facet | Plant cell culture Plant tissue culture Zellkultur Gewebekultur Pflanzenzelle Pflanzen Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018713828&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT daveymichaelr plantcellcultureessentialmethods AT anthonypaul plantcellcultureessentialmethods |