Fundamentals of microfluidics and lab on a chip for biological analysis and discovery:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boca Raton [u.a.]
CRC Press
2010
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 398 S. Ill., graph. Darst. |
| ISBN: | 9781439818558 143981855X |
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MARC
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| 245 | 1 | 0 | |a Fundamentals of microfluidics and lab on a chip for biological analysis and discovery |c Paul C. H. Li |
| 264 | 1 | |a Boca Raton [u.a.] |b CRC Press |c 2010 | |
| 300 | |a XV, 398 S. |b Ill., graph. Darst. | ||
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Record in the Search Index
| _version_ | 1804140815084158976 |
|---|---|
| adam_text | Contents
Preface
.......................:.......................................................................................................................xi
Acknowledgments
..........................................................................................................................xiii
About the Author
.............................................................................................................................xv
Chapter
1
Introduction
..................................................................................................................1
Chapter
2
Micromachining Methods
............................................................................................3
2.1
Micromachining of Silicon
................................................................................3
2.1.1
Etching of Si
Microchannels................................................................3
2.1.2
Bonding of Si Chips
.............................................................................5
2.2
Micromachining of Glass
..................................................................................5
2.2.1
Etching of Glass
Microchannels..........................................................7
2.2.2
Drilling of Glass for Access-Hole Formation
....................................11
2.2.3
Glass Bonding
....................................................................................13
2.3
Micromachining of Fused Quartz (or Fused Silica)
........................................15
2.3.1
Fused Quartz Channel Etching and Hole Drilling
.............................15
2.3.2
Bonding of Fused Quartz Chips
.........................................................15
2.4
Micromachining of Polymeric Chips
..............................................................16
2.4.1
Casting
................................................................................................17
2.4.2
Injection Molding
...............................................................................24
2.4.3
Ablation
..............................................................................................24
2.4.4
Wire Imprinting
.................................................................................27
2.4.5
Compression Molding
........................................................................28
2.4.6
Photopolymerization
..........................................................................29
2.4.7
Other Plastic Micromachining Processes
..........................................31
2.4.8
Problems Encountered in Polymeric Chips
........................................33
2.4.8.1
Optical Properties
...............................................................33
2.4.8.2
Electrical Properties
...........................................................33
2.4.8.3
Thermal Properties
.............................................................33
2.4.8.4
Mechanical Properties
........................................................34
2.4.8.5
Surface Properties
...............................................................35
2.4.8.6
Solvent-Resistant Properties
...............................................37
2.5
Metal Patterning
..............................................................................................37
2.6
World-to-Chip Interface
..................................................................................40
2.7
ProblemSets
....................................................................................................43
Chapter
3
Microfluidic Flow
.......................................................................................................47
3.1
Liquid Pumping Methods
................................................................................47
3.1.1
Electroosmotie Flow (EOF)
...............................................................47
3.1.2
Pressure-Driven Flow
.........................................................................47
3.1.3
Centrifugal Pumping
..........................................................................50
3.1.4
Alternative Pumping Principles
.........................................................50
3.1.4.1
EOF-Induced Flow
.............................................................50
VII
VIII
Contents
3.1.4.2
Electrochemical
Bubble Generation
...................................51
3.1.4.3
Thermally Induced Pumping
..............................................54
3.1.4.4
Surface Energy
...................................................................54
3.1.4.5
Pneumatic Control
..............................................................54
3.1.4.6
Magnetohydrodynamic
(MHD)
Pumping
..........................54
3.1.4.7
Evaporation
.........................................................................56
3.1.4.8
Miscellaneous Pumping Methods
......................................56
3.1.5
Microfluidic Flow Modeling Study
....................................................57
3.2
Microfluidic Flow Control
...............................................................................59
3.2.1
Surface Modifications for Flow Control
............................................59
3.2.2
Laminar Flow for Liquid Extraction and
Microfabrication
...............61
3.2.3
Generation of Concentration and Temperature Gradients
.................63
3.2.4
Flow Switching
...................................................................................66
3.2.5
Fluid Mixing
......................................................................................75
3.2.5.1
Diffusive Mixing
................................................................75
3.2.5.2
Chaotic Advection
..............................................................77
3.2.5.3
Oscillating Flow
.................................................................80
3.2.5.4
Acoustic Mixing
.................................................................81
3.2.5.5
Other Mixing Methods
.......................................................83
3.2.6
Liquid Dispensing
..............................................................................84
3.3
ProblemSets
....................................................................................................86
Chapter
4
Sample Introduction
...................................................................................................87
4.1
Electrokinetic Injection
...................................................................................87
4.1.1
Pinched Injection
................................................................................88
4.1.2
Gated Injection
...................................................................................93
4.2
Hydrodynamic Injection
..................................................................................99
4.3
Other Sample Injection Methods
...................................................................102
4.4
ProblemSets
..................................................................................................103
Chapteră
Sample Preconcentration
..........................................................................................105
5.1
Sample Stacking
............................................................................................105
5.2
Extraction
......................................................................................................106
5.3
Porous Membrane
..........................................................................................110
5.4
Other Preconcentration Methods
...................................................................113
5.5
ProblemSets
..................................................................................................119
Chapter
6
Separation
........................................... ......................................................................121
6.1
Gas Chromatography (GC)
............................................................................121
6.2
Capillary Electrophoresis
(CE)
.....................................................................121
6.2.1
Free-Solution Capillary Electrophoresis (FSCE)
.............................121
6.2.2
Capillary Gel Electrophoresis (CGE)
...............................................129
6.2.3
Micellar Electrokinetic Capillary Chromatography (MECC)
.........133
6.2.4
Isotachophoresis (ITP)
.....................................................................135
6.2.5
Capillary Electrochromatography
(CEC)
........................................137
6.2.6
Synchronized Cyclic Capillary Electrophoresis (SCCE)
.................142
6.2.7
Free-Flow Electrophoresis (FFE)
.......... ...........................................145
6.2.8
Derivatizations for
CE
for Separations
............................................147
Contents ix
6.2.8.1 Precolumn Derivatization.................................................147
6.2.8.2 Postcolumn Derivatization................................................148
6.3 Chromatographie Separations.......................................................................148
6.4 Coupled Separations......................................................................................150
6.4.1
Fraction Collection...........................................................................
150
6.4.2 Two-Dimensional Separations.........................................................153
6.5 ProblemSets..................................................................................................158
Chapter
7
Detection Methods
...................................................................................................159
7.1
Optical Detection Methods
............................................................................159
7.1.1
Fluorescence Detection
....................................................................159
7.1.1.1
Single-Channel Fluorescence Detection
..........................159
7.1.1.2
Scanning Detector
............................................................161
7.1.1.3
Background Reduction
.....................................................162
7.1.1.4
Photobleaching Effect
.......................................................163
7.1.1.5
Integrated Fluorescent Detector
.......................................163
7.1.2
Indirect Fluorescent Detection
.........................................................165
7.1.3
Multiple-Point Fluorescent Detection
..............................................165
7.1.4
Absorbance Detection
......................................................................168
7.1.5
Plasma Emission Detection
..............................................................171
7.1.6
Chemiluminescence (CL) Detector
..................................................173
7.1.7
Refractive Index (RI)
.......................................................................176
7.1.8
Thermal Lens Microscope (TLM)
...................................................177
7.1.9
Raman Scattering
.............................................................................177
7.1.10
Surface-Plasmon Resonance
............................................................178
7.1.11
Infrared Detection
............................................................................178
7.2
Electrochemical (EC) Detection
...................................................................178
7.2.1
Amperometric Detection
..................................................................178
7.2.2
Voltammetric Detection
...................................................................184
7.2.3
Potentiometric Detection
..................................................................185
7.2.4
Conductivity Detection
.....................................................................187
7.3
Mass Spectrometry (MS)
..............................................................................189
7.3.1
Electrospray Ionization (ESI)
...........................................................189
7.3.2
Matrix-Assisted Laser Desorption Ionization (MALDI)
.................194
7.4
Other Detection Methods
..............................................................................199
7.4.1
Thermal Detection
................................................................ ...........199
7.4.2
Acoustic Wave Detection
.................................................................199
7.4.3
Nuclear Magnetic Resonance (NMR)
..............................................200
7.5
ProblemSets
..................................................................................................209
Chapter
8
Applications to Cellular/Particle Analysis
...............................................................213
8.1
Retention of Cells and Particles
....................................................................213
8.1.1
Slit-Type Filters
................................................................................213
8.1.2
Weir-Type Filters
..............................................................................215
8.1.3
Cell Adhesion
...................................................................................222
8.1.4
Polymer Entrapment
.........................................................................226
8.1.5
Three-Dimensional Flow Control
....................................................227
8.1.6
Optical Trapping of Cells
.................................................................229
8.1.7
Dielectrophoresis
(DEP)
...................................................................231
x
Contents
8.2
Studies of Cells in a Flow
..............................................................................236
8.3
Other Cell Operations
....................................................................................243
8.3.1
Cell Culture
......................................................................................243
8.3.2
Electroporation
.................................................................................246
8.3.3
Chip Cleaning and Sterilization
.......................................................246
8.3.4
Prevention of Cell Adhesion
.............................................................246
8.4
Problem Sets
..................................................................................................247
Chapter
9
Applications to Nucleic Acids Analysis
...................................................................249
9.1
Nucleic Acids Extraction and Purification
....................................................249
9.2
Nucleic Acids Amplification
.........................................................................249
9.2.1 DNA
Amplification
..........................................................................249
9.2.1.1
Polymerase Chain Reaction (PCR)
...................................249
9.2.1.2
Surface Passivation of PCR Chambers
.............................251
9.2.1.3
Integrated
DNA
Analysis Microsystems
..........................256
9.2.1.4
Real-Time
PCR
.................................................................259
9.2.1.5
Flow-Through PCR
...........................................................260
9.2.1.6
Other
DNA
Amplification Techniques
.............................263
9.2.2
RNA
Amplification
..........................................................................264
9.3 DNA
Hybridization
.......................................................................................265
9.3.1
MicroChannel
DNA
Hybridization
...................................................265
9.3.2
Microarray
DNA
Hybridization
.......................................................268
9.4
Other Nucleic Acid Applications
...................................................................269
9.4.1 DNA
Sequencing
..............................................................................269
9.4.2
Genetic Analysis
..............................................................................272
9.4.3
Separation of Large
DNA
Molecules
...............................................275
9.5
ProblemSets
..................................................................................................284
Chapter
10
Applications to Protein Analysis
..............................................................................285
10.1
Immunoassay
.................................................................................................285
10.1.1
Homogeneous Immunoassay
............................................................285
10.1.2
Heterogeneous Immunoassay
.......................................................-....290
10.2
Protein Separation
.........................................................................................295
10.3
Enzymatic Assays
..........................................................................................297
10.3.1
Assay of the Enzymes
......................................................................297
10.3.2
Assay of Analytes after Enzymatic Reactions
.................................302
10.4
ProblemSets
..................................................................................................306
References
.....................................................................................................................................307
Glossary
........................................................................................................................................357
Appendix: Analytical Applications of Microfluidic Technology
.............................................365
Index
..............................................................................................................................................385
|
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| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610.28 |
| dewey-search | 610.28 |
| dewey-sort | 3610.28 |
| dewey-tens | 610 - Medicine and health |
| discipline | Physik Biologie Elektrotechnik / Elektronik / Nachrichtentechnik Medizin |
| format | Book |
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| id | DE-604.BV035845065 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:05:58Z |
| institution | BVB |
| isbn | 9781439818558 143981855X |
| language | English |
| lccn | 2009035035 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018703281 |
| oclc_num | 320801274 |
| open_access_boolean | |
| owner | DE-29T DE-355 DE-BY-UBR DE-703 |
| owner_facet | DE-29T DE-355 DE-BY-UBR DE-703 |
| physical | XV, 398 S. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | CRC Press |
| record_format | marc |
| spelling | Li, Paul C. H. 1962- Verfasser (DE-588)1048245055 aut Fundamentals of microfluidics and lab on a chip for biological analysis and discovery Paul C. H. Li Boca Raton [u.a.] CRC Press 2010 XV, 398 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biochips Microfluidics Biochip (DE-588)4270225-2 gnd rswk-swf Mikrofluidik (DE-588)4803438-1 gnd rswk-swf Biochip (DE-588)4270225-2 s Mikrofluidik (DE-588)4803438-1 s DE-604 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018703281&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Li, Paul C. H. 1962- Fundamentals of microfluidics and lab on a chip for biological analysis and discovery Biochips Microfluidics Biochip (DE-588)4270225-2 gnd Mikrofluidik (DE-588)4803438-1 gnd |
| subject_GND | (DE-588)4270225-2 (DE-588)4803438-1 |
| title | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery |
| title_auth | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery |
| title_exact_search | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery |
| title_full | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery Paul C. H. Li |
| title_fullStr | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery Paul C. H. Li |
| title_full_unstemmed | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery Paul C. H. Li |
| title_short | Fundamentals of microfluidics and lab on a chip for biological analysis and discovery |
| title_sort | fundamentals of microfluidics and lab on a chip for biological analysis and discovery |
| topic | Biochips Microfluidics Biochip (DE-588)4270225-2 gnd Mikrofluidik (DE-588)4803438-1 gnd |
| topic_facet | Biochips Microfluidics Biochip Mikrofluidik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018703281&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lipaulch fundamentalsofmicrofluidicsandlabonachipforbiologicalanalysisanddiscovery |