Biopharmaceuticals in plants: toward the next century of medicine
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| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
Boca Raton, Fla. [u.a.]
CRC Press
2010
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Ill., graph. Darst. |
| Physical Description: | XVII, 206 S. |
| ISBN: | 9781439804742 1439804745 |
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| 100 | 1 | |a Hefferon, Kathleen L. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Biopharmaceuticals in plants |b toward the next century of medicine |c Kathleen Laura Hefferon |
| 264 | 1 | |a Boca Raton, Fla. [u.a.] |b CRC Press |c 2010 | |
| 300 | |a XVII, 206 S. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Ill., graph. Darst. | ||
| 650 | 4 | |a Biological products | |
| 650 | 4 | |a Transgenic plants | |
| 650 | 4 | |a Plant genetic regulation | |
| 650 | 4 | |a Pharmaceutical technology | |
| 650 | 4 | |a Plant extracts | |
| 650 | 4 | |a Biological products | |
| 650 | 4 | |a Pharmaceutical technology | |
| 650 | 4 | |a Plant extracts | |
| 650 | 4 | |a Plant genetic regulation | |
| 650 | 4 | |a Transgenic plants | |
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Record in the Search Index
| _version_ | 1804140793189892096 |
|---|---|
| adam_text | Titel: Biopharmaceuticals in plants
Autor: Hefferon, Kathleen L.
Jahr: 2010
Contents
Preface xiii
Acknowledgments xv
The Author xvii
Chapter 1 History of Plants in Production of Biopharmaceuticals 1
1.1 Introduction 1
1.2 History of Vaccine Development 2
1.3 History of Vaccine Proteins Produced in Plants 3
1.4 Transformation of Plant Tissue 5
1.5 Agrobacterium tumefaciens-Mediated Plant
Transformation 6
1.5.1 History and Concept of Agrobacterium
tumefaciens for Plant Transformation 6
1.5.2 Molecular Basis of Agrobacterium-
Mediated Transformation 7
1.5.3 Vir Proteins Used in Agrobacterium-
Mediated Transformation 9
1.5.3.1 VirA and VirG Proteins 9
1.5.3.2 VirD4 and VirB Proteins 9
1.5.3.3 VirD2 and VirE2 Proteins 10
1.5.3.4 VirF Protein 11
1.5.4 Other Genes That Play a Role in
Agrobacterium-Mediated Transformation.... 12
1.5.5 Integration of T-DNA into the Host
Genome 13
1.5.6 Host Range and Susceptibility of Plants
to Agrobacterium Transformation 13
1.6 Other Transformation Techniques 14
1.6.1 Biolistic Delivery 14
1.6.2 Polyethylene Glycol (PEG) and Other
Transformation Methods 15
1.7 Problems Associated with Transformation
Techniques 15
1.8 Transformation of Cereals 16
1.9 Hairy Root Transformation 17
1.10 Selectable Marker Genes 17
1.11 Regulation of Transgene Expression 18
v
vi Contents
1.11.1 Promoter and Terminator Elements 18
1.11.2 Constitutive Promoters 18
1.11.2.1 35S CaMV Promoter 18
1.11.2.2 Ubil Promoter 19
1.11.3 Plant Viral Leader Sequences 19
1.11.4 Chemical-Inducible and Developmental
Promoters for Temporal or Spatial Gene
Expression 19
1.11.5 Construction of Synthetic Genes 21
1.11.6 Targeting of Protein to Plant Cell
Organelles 21
1.11.7 Transgenic Plants and Gene Silencing 21
1.12 Conclusions 23
References 23
Chapter 2 Transgenic Plants Expressing Vaccine and Therapeutic
Proteins 27
2.1 Introduction 27
2.2 Transgenic Plants Expressing Vaccines against
Diarrheal Diseases 28
2.2.1 LT-B and CT-B 28
2.2.2 Norwalk Virus 30
2.2.3 Rotavirus 31
2.2.4 Hepatitis B Virus (HBV) 31
2.2.5 Tuberculosis (TB) 33
2.2.6 Human Immunodeficiency Virus (HIV) 33
2.2.7 Plague Vaccine 33
2.2.8 Measles Virus 34
2.2.9 Papillomavirus 34
2.2.10 Smallpox Virus 35
2.2.11 Influenza Virus 36
2.3 Animal Vaccines Produced in Plants 36
2.3.1 Foot and Mouth Disease Virus 36
2.3.2 Bovine Rotavirus 37
2.3.3 Transmissible Gastroenteritis Virus 37
2.3.4 Infectious Bursal Disease Virus 37
2.3.5 Newcastle Disease Virus 38
2.3.6 Rabbit Hemorrhagic Disease Virus
(RHDV) 38
2.3.7 Canine Parvovirus (CPV) 38
2.4 The Use of Antibodies in Plants as
Immunotherapeutic Agents 39
Contents vii
2.4.1 Monoclonal Antibodies Expressed in
Plants against Rabies Virus 41
2.5 Other Biopharmaceuticals and Therapeutic
Agents Produced in Plants 42
2.5.1 Interleukins 44
2.5.2 Taxadiene 44
2.5.3 Allergens 45
2.5.4 Interferon and Other Antiviral Proteins 45
2.5.5 Birth Control 46
2.6 Enhancement of Plants for Nutritional or
Medicinal Purposes (Nutriceuticals) 46
2.6.1 Golden Rice 46
2.6.2 Other Nutriceuticals Produced in Plants 47
2.6.3 Enhancing the Nutritional Content of
Plants by Alteration of Their Natural
Metabolic Pathways 48
2.7 Conclusions 49
References 49
Chapter 3 Chloroplast Engineering and Production of
Biopharmaceuticals 57
3.1 Introduction 57
3.2 Differences between Nuclear and Plastid
Transformations 59
3.3 Plastid Transformation 59
3.3.1 Basic Concept 59
3.3.2 Plastid Transformation Systems 62
3.3.2.1 Biolistic Delivery of Foreign
DNA 62
3.3.2.2 Transformation by PEG 62
3.3.2.3 Microinjection 62
3.3.2.4 DNA Transfer Mediated by
T-DNA of Agrobacterium
tumefaciens and Other
Approaches 63
3.3.3 Vectors and Selectable Markers Used 64
3.3.4 Advantages of and Stumbling Blocks to
Plastid Transformation 65
3.3.4.1 Advantages 65
3.3.4.2 Limitations to Chloroplast
Engineering 66
3.3.5 Transformation in Other Crops 67
viii Contents
3.4 History of Plastid Transformation and
Biotechnological Applications 68
3.5 Vaccines and Therapeutic Proteins Produced in
Chloroplasts 69
3.5.1 Human Somatotropin (hST) 69
3.5.2 Cholera Toxin (CT) and E. coli Heat-
Labile Toxin (LT) 70
3.5.3 Tetanus Toxin 71
3.5.4 Canine Parvovirus 72
3.5.5 Anthrax Protective Antigen (PA) 73
3.5.6 Hepatitis E ORF2 Protein 73
3.6 Conclusions 74
References 74
Chapter 4 Plant Viral Expression Vectors and Production of
Biopharmaceuticals in Plants 77
4.1 Introduction 77
4.2 Plant RNA Virus Expression Systems 78
4.2.1 Epitope Presentation Systems 78
4.2.1.1 Cowpea Mosaic Virus 78
4.2.1.2 Tobacco Mosaic Virus 80
4.2.1.3 Potato Virus X 80
4.2.2 Polypeptide Expression Systems 81
4.2.2.1 Tobacco Mosaic Virus 81
4.2.2.2 Potato Virus X 81
4.2.2.3 Cowpea Mosaic Virus (CPMV) 82
4.2.2.4 Plum Pox Virus (PPV) 82
4.2.2.5 Cucumber Mosaic Virus (CMV).... 83
4.2.3 Plant DNA Viruses as Expression
Vectors 83
4.2.4 Novel Strategies for the Development of
Viral Vectors 84
4.2.5 Commercial Uses of Virus Expression
Vectors 87
4.2.5.1 Vaccines and Therapeutic
Proteins 87
4.2.5.2 Virus Expression Vectors and
Virus-Induced Gene Silencing 91
4.2.5.3 Virus Expression Vectors and
High-Throughput Gene Function
Discovery 92
4.3 Summary 92
Contents jx
References 93
Chapter 5 Glycosylation of Therapeutic Proteins in Plants 99
5.1 Introduction 99
5.2 iV-Glycosylation 101
5.3 Differences in N-Glycosylation Patterns between
Plants and Mammals 102
5.4 Plant-Made Pharmaceuticals and Plant Allergens... 102
5.5 Strategies to Humanize Recombinant Proteins in
Plants 103
5.5.1 Retention of Glycoproteins within the ER... 103
5.5.2 Development of Plants that Lack 0(1,2)-
Xylose and Core a(l,3)-Fucose 106
5.5.3 Expression of Human (3(1,4)-
Galactosyltransferase in Plants 107
5.6 O-Glycosylation 108
5.7 Sialic Acid in Plants 109
5.8 Glycosylation of Immunoglobulins Produced in
Plants 109
5.9 Other Therapeutic Proteins Ill
5.10 Conclusions 112
References 112
Chapter 6 Scale-Up of Plant-Derived Pharmaceuticals: Prospects
for Commercial Production and for Global Health 117
6.1 Introduction 117
6.2 Plant-Made Pharmaceuticals on the Market 121
6.3 Scale-Up of Production of Biopharmaceuticals in
Plants 121
6.3.1 Production via Open Fields 121
6.3.2 Plant Species Enlisted for Open Field
Production 122
6.3.2.1 Plant Production Platforms for
Human Health 122
6.3.2.2 Plant Production Platforms for
Animal Health 123
6.4 Application of Virus Expression Vectors for
Large-Scale Field Trials 123
6.5 Production of Biopharmaceuticals in Plant
Suspension Cells 126
x Contents
6.6 Comparison of Cell Culture over Whole Plants
for Biopharmaceutical Production 130
6.7 Other Expression Systems for Large-Scale
Production of Biopharmaceuticals 131
6.7.1 Hairy Root Culture and Rhizosecretion 131
6.7.2 Moss 132
6.7.3 Aquatic Plants 133
6.8 Downstream Processing of Plant-Derived
Biopharmaceuticals 134
6.9 Driving Forces behind Plant-Made
Biopharmaceuticals: The General Market
Environment 137
6.9.1 Commercialization of Plant-Derived
Products and the Corporate Arena 137
6.9.2 Philanthropic Uses for Plant-Derived
Pharmaceuticals 138
6.9.3 Plant-Derived Biopharmaceuticals as a
Defense against Biological Warfare
Agents 140
6.10 Conclusions 141
References 141
Chapter 7 The Immune Response to Plant-Derived
Pharmaceuticals 147
7.1 Introduction 147
7.2 The Immune System in General 148
7.3 T Cells 149
7.4 B Cells 149
7.5 Antibodies and the Immune Response 151
7.6 The Concept of Vaccination and Immune
Imprinting 154
7.7 Organization of the Mucosal Immune System 155
7.8 Secretory IgA and the Mucosal Epithelium 156
7.9 Mucosal Immune Response of the Respiratory
Tract 157
7.10 Oral Tolerance 158
7.10.1 Adjuvants and Other Strategies to
Enhance Mucosal Immunity 158
7.10.2 Adjuvants and the Mucosal Surface 158
7.10.3 CT-B as Oral Adjuvant 160
7.10.4 Saponins 161
Contents xi
7.11 Preclinical and Clinical Trials Involving Plant-
Derived Vaccines 162
7.12 Diarrheal Diseases 162
7.13 Hepatitis B Virus (HBV) 165
7.14 Rabies Virus 166
7.15 Human Papillomavirus (HPV) 166
7.16 Anthrax 167
7.17 Severe Acute Respiratory Syndrome (SARS) 167
7.18 Measles Virus 168
7.19 Influenza Virus 169
7.20 Swine-Transmissible Gastroenteritis Virus 169
7.21 Canine Parvovirus 169
7.22 Oral Tolerance to Antigens 170
7.23 Conclusions 170
References 171
Chapter 8 Risk Analysis and Safety of Plant-Made
Biopharmaceuticals 177
8.1 Introduction 177
8.2 Risk Analysis and Plant-Based
Biopharmaceuticals 178
8.3 Regulation of Plant-Made Biopharmaceuticals 179
8.4 Quality Control and the Manufacturing of the
Product 181
8.4.1 Characterization of the Product 183
8.4.2 Protein Instability 183
8.4.3 Genetic Stability 184
8.5 Impact of Plant-Made Biopharmaceuticals on
Human Health 184
8.5.1 Transgenic Crops as a Potential Hazard
to Diet 184
8.5.2 Allergenicity 185
8.6 Impact of Plant-Made Biopharmaceuticals on the
Environment 185
8.6.1 Gene Containment 186
8.6.1.1 Spatial and Temporal Separation... 186
8.6.1.2 Prevention of Outcrossing 187
8.6.1.3 Equipment and Training 187
8.6.2 Nontarget Organisms 188
8.7 Avoiding Transgene Silencing 189
8.7.1 Improvements in the Design of
Transformation Vectors 189
xii Contents
8.7.2 Examination of the Site of Integration
and Base Competition of the Transgene
and Surrounding Regions 189
8.7.3 Method of Transformation and Selection
of Host Plant Species 190
8.7.4 Growth Conditions of Transgenic Plants 190
8.7.5 Copy Number of Transgene 190
8.8 Conclusions 190
References 191
Chapter 9 Epilogue: The Future 195
9.1 The Current State of Plant-Made
Biopharmaceuticals 195
Index 197
|
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| spelling | Hefferon, Kathleen L. Verfasser aut Biopharmaceuticals in plants toward the next century of medicine Kathleen Laura Hefferon Boca Raton, Fla. [u.a.] CRC Press 2010 XVII, 206 S. txt rdacontent n rdamedia nc rdacarrier Ill., graph. Darst. Biological products Transgenic plants Plant genetic regulation Pharmaceutical technology Plant extracts Biopharmazie (DE-588)4006890-0 gnd rswk-swf Biopharmazie (DE-588)4006890-0 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018689404&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hefferon, Kathleen L. Biopharmaceuticals in plants toward the next century of medicine Biological products Transgenic plants Plant genetic regulation Pharmaceutical technology Plant extracts Biopharmazie (DE-588)4006890-0 gnd |
| subject_GND | (DE-588)4006890-0 |
| title | Biopharmaceuticals in plants toward the next century of medicine |
| title_auth | Biopharmaceuticals in plants toward the next century of medicine |
| title_exact_search | Biopharmaceuticals in plants toward the next century of medicine |
| title_full | Biopharmaceuticals in plants toward the next century of medicine Kathleen Laura Hefferon |
| title_fullStr | Biopharmaceuticals in plants toward the next century of medicine Kathleen Laura Hefferon |
| title_full_unstemmed | Biopharmaceuticals in plants toward the next century of medicine Kathleen Laura Hefferon |
| title_short | Biopharmaceuticals in plants |
| title_sort | biopharmaceuticals in plants toward the next century of medicine |
| title_sub | toward the next century of medicine |
| topic | Biological products Transgenic plants Plant genetic regulation Pharmaceutical technology Plant extracts Biopharmazie (DE-588)4006890-0 gnd |
| topic_facet | Biological products Transgenic plants Plant genetic regulation Pharmaceutical technology Plant extracts Biopharmazie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018689404&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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