Antibody phage display: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
New York
Humana Press
2009
|
| Edition: | 2. ed. |
| Series: | Methods in molecular biology
562 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | X, 240 S. Ill., graph. Darst. |
| ISBN: | 9781603273015 |
Staff View
MARC
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| 245 | 1 | 0 | |a Antibody phage display |b methods and protocols |c ed. by Robert Aitken |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a New York |b Humana Press |c 2009 | |
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Record in the Search Index
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|---|---|
| adam_text | Titel: Antibody phage display
Autor: Aitken, Robert
Jahr: 2009
Contents
Preface p
Contributors ix
1 Antibody Phage Display: Overview of a Powerful Technology
that Has Quickly Translated to the Clinic 1
Beatrix Kotlan and Mark C. Glassy
2 Design and Construction of Synthetic Phage-Displayed Fab Libraries 17
Jenny Bostrom and Germaine Fuh
3 Affinity Isolation of Antigen-Specific Circulating B Cells for
Generation of Phage Display-Derived Human Monoclonal Antibodies 37
HenrikJ. Ditzel
4 Construction of Phage Antibody Repertoires
from the Blood of West Nile Virus-Infected Donors 45
Mark Throsby and John de Kruif
5 Anti-P2 GP-I and Anti-prothrombin Antibodies Generated
by Phage Display 61
Javier Cabiedes and Antonio R. Cabral
6 The Generation and Selection of Single-Domain,
V Region Libraries from Nurse Sharks 71
Martin F. Flajnik and Helen Dooley
7 The Isolation of scFvs Against Small Target Molecules 83
Iain Shaw and Marian Kane
8 Display and Selection of scFv Antibodies on HEK-293T Cells 99
Mitchell Ho and Ira Pastan
9 Isolation of scFvs that Inhibit the NS3 Protease of Hepatitis C Virus
by a Combination of Phage Display and a Bacterial Genetic Screen 115
Limor Nahary, Alia Trahtenherts, and Itai Benhar
10 Guided Selection Methods Through Chain Shuffling 133
Bao Guo-qiang and He Xian-Li
11 Sequential Antigen Panning for Selection of Broadly Cross-Reactive
HIV-1-Neutralizing Human Monoclonal Antibodies 143
Mei-Tun Zhang and Dimiter S. Dimitrov
12 Selection of Antibodies Able to Rapidly Enter Mammalian Cells 155
Marie-Alix Poul
13 Identification of the Specificity of Isolated Phage Display
Single-Chain Antibodies Using Yeast Two-Hybrid Screens 165
Nicolaj Rasmussen and HenrikJ. Ditzel
14 Generation of Bispecific and Tandem Diabodies 177
Sergey M. Kipriyanov
vii
viii Contents
15 Intrabody Expression in Eukaryotic Cells 195
Laurence Guglielmi and Pierre Martineau
16 High-Level Periplasmic Expression and Purification of scFvs 205
Sergey M. Kipriyanov
17 Expression of Single-Chain Fv Fragments in E. coli Cytoplasm 215
Laurence Guglielmi and Pierre Martineau
18 High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris. 225
Leonardo M. Damasceno, Frank Lee, Gerd Ritter, Lloyd Old,
and Carl Batt
Index 237
|
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| building | Verbundindex |
| bvnumber | BV035766061 |
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| ctrlnum | (OCoLC)495319437 (DE-599)BSZ306523728 |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| isbn | 9781603273015 |
| language | English |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Antibody phage display methods and protocols ed. by Robert Aitken 2. ed. New York Humana Press 2009 X, 240 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 562 Springer protocols Anticorps monoclonal Anticorps monoclonaux ram Bactériophages Monoklonaler Antikörper (DE-588)4040094-3 gnd rswk-swf Phagen-Display-Bibliothek (DE-588)4512153-9 gnd rswk-swf Bakteriophagen (DE-588)4125508-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Monoklonaler Antikörper (DE-588)4040094-3 s Bakteriophagen (DE-588)4125508-2 s DE-604 Phagen-Display-Bibliothek (DE-588)4512153-9 s 1\p DE-604 Aitken, Robert Sonstige oth Methods in molecular biology 562 (DE-604)BV035362695 562 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018625850&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Antibody phage display methods and protocols Methods in molecular biology Anticorps monoclonal Anticorps monoclonaux ram Bactériophages Monoklonaler Antikörper (DE-588)4040094-3 gnd Phagen-Display-Bibliothek (DE-588)4512153-9 gnd Bakteriophagen (DE-588)4125508-2 gnd |
| subject_GND | (DE-588)4040094-3 (DE-588)4512153-9 (DE-588)4125508-2 (DE-588)4143413-4 |
| title | Antibody phage display methods and protocols |
| title_auth | Antibody phage display methods and protocols |
| title_exact_search | Antibody phage display methods and protocols |
| title_full | Antibody phage display methods and protocols ed. by Robert Aitken |
| title_fullStr | Antibody phage display methods and protocols ed. by Robert Aitken |
| title_full_unstemmed | Antibody phage display methods and protocols ed. by Robert Aitken |
| title_short | Antibody phage display |
| title_sort | antibody phage display methods and protocols |
| title_sub | methods and protocols |
| topic | Anticorps monoclonal Anticorps monoclonaux ram Bactériophages Monoklonaler Antikörper (DE-588)4040094-3 gnd Phagen-Display-Bibliothek (DE-588)4512153-9 gnd Bakteriophagen (DE-588)4125508-2 gnd |
| topic_facet | Anticorps monoclonal Anticorps monoclonaux Bactériophages Monoklonaler Antikörper Phagen-Display-Bibliothek Bakteriophagen Aufsatzsammlung |
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