Hydrophobins in wood biology and biotechnology:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. und engl. Sprache |
| Beschreibung: | XIV, 236 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | Titel: Hydrophobins in wood biology and biotechnology
Autor: Peddireddi, Sudhakar
Jahr: 2008
Table of contents
Table of contents
Acknowledgements i
Zusammenfassung iii
Summary vi
Table of contents ix
1. General Introduction 1
1.1 General background and aim of this thesis 1
1.2 The fungus Schizophyllum commune 2
13 Schizophyllum commune growth on wood 3
1.4 Application of hydrophobins with wood 4
1.5 References 4
2. Growth of Schizophyllum commune in natural environments and
interaction with other fungi 9
2.1 Abstract 9
2.2 Introduction 10
2.3 Materials and Methods 13
2.3.1 Juglans tree 13
2-1.2 Isolation of fungal strains from wood 13
233 Fungal strains 13
2J.4 Growth conditions 14
2.3.5 Juglans decay test 14
23.6 Interaction studies 15
2.3.7 Statistical analysis 15
2.4 Results 16
2.4.1 £ commune as a pathogen on a Jmgbms ailanlifolia tree 16
2.4.1.1 Fungal isolations 18
2.4.2 Wood decay test 33
2.4 J Fungal interactions 41
2.5 Discussion 58
2.6 References 53
Table of contents
3. Wood decay by Schizophyttum commune 57
3.1 Abstract 57
3.2 Introduction 58
33 Materials and methods 60
3 J.I Fungal strains 60
3.3.2 Growth conditions 60
333 Wood decay tests 61
333.1 Wood block test 61
333.2 Saw dust decay test 61
33.4 Bending strength test of wood 62
3.4 Results 62
3.4.1 Wood block decay tests 62
3.4.2 Decay of saw dusts from different tree species by
£ commune strains 72
3.5 Discussion 85
3.6 References 87
4. FTIR spectroscopy of a S. commune moookaryotic wild type and a
coisogenic Sc3 hydrophobia mutant 91
4.1 Abstract 91
4.2 Introduction 92
4.2.1 FTIR Spectroscopy 92
4.2.2 IR Spectral regions for microorganisms 92
4.23 FTIR studies on bacteria, yeast and ascomycetes 93
4.2.4 FTIR studies on basidromycetes 94
43 Materials and methods 95
43.1 Fungal strains 9S
43.2 Growth conditions 95
4.33 Culture harvest and mycelia grinding 95
43.4 ATR - FTIR Spectroscopy 96
43.5 Data analysis 96
43.6 Statistical analysis 96
4.4 Results 99
Table of contents
4.5 Discussion 113
4.6 References 113
4.7 Appendix to chapter 4 118
S. FTIR microscopy of S. commune in wood 128
5.1 Abstract 128
5.2 Introduction 129
5.3 Material and methods 129
5.3.1 Fungal strains 129
5.3.2 Growth conditions 130
533 Wood block test 130
5.3.4 Light Microscopy 131
53.5 Sample preparation for FTIR microscopy 131
53.6 FTIR microscopy 131
53.7 Spectral data analysis 132
53.8 Statistical analysis 132
5.4 Results 132
5.4.1 Wood decay test and light microscopy 132
5.4.2 Discrimination of fungi and wood by FTIR analysis 135
5.43 Discrimination of fungal strains 140
5.4.4 Distribution of commune mycelium within beech wood 143
5.4.5 Quantification of protein and polysacchwide contents
in the mycelium 144
5.5 Discussion 148
5.6 References 150
6. Hydrophobim in wood technology 153
6.1 Abstract 153
6.2 Introduction 154
63 Material and methods 155
63.1 Fungal strain 155
63.2 Growth conditions 156
633 Culture harvest 156
Table of contents
6J.4 Purification of SC3 bydropbobin 156
6.3.5 Analytical procedures 157
6.3.6 Coating SC3 hydrophobin on Teflon, form-war film and glass 157
63.7 Coating beech wood with SC3 hydrophobin 158
6.3.8 Water absorption measurements 158
63.9 FTIR-spectroscopy 158
6.3.9.1 Purified hydrophobin 158
6.3.9.2 Control Teflon and SC3 treated Teflon 159
6.3.9.3 Control beech wood and SC3 treated beech wood 159
63.9.4 FTIR-spectral analysis 160
6.3.9.5 Wood vessel measurement 160
63.9.6 Statistical analysis 161
6.4 Results 161
6.4.1 Purified hydrophobin 161
6.4.2 SC3 coatings on hydrophobic and hydropbilic materials 163
6.43. Hydrophobin activity on beech wood surface 165
6.4.4. FTIR-spectroscopy 168
6.5 Discussion 169
6.6 References 172
6.7 Appendix to chapter 6 177
7. Class I hydrophobias from C cinerea and P. ostreatus in wood technology 180
7.1 Abstract 180
7.2 Introduction 181
73 Materials and methods 181
73.1 Fungal strains 181
73.2 Growth conditions 182
733 Culture harvest 182
73.4 Purification of hydrophobin from C. cinerea and
P. mtnatms aerial hyphae 182
73.5 Analytical procedures 183
73.6 Coatiag beech wood with hydropbobins of Coprinopsi
and PleurotHS 183
Table of contents
7.3.7 Water absorption measurements 184
7.3.8 FTIR-spectroscopy 184
7J.8.1 Purified hydrophobin from C cinerea and P. ostreatm 184
7 J.8.2 Control beech wood, C. cinerea and
P. ostreatm hydrophobins treated beech wood 185
7J.8.3 FTIR-spectral analysis 185
7J.8.4 Statistical analysis 185
7.4 Results 185
7.4.1 Purified hydrophobins from vegetative mycelium of
Coprinopsis and Plturotus 185
7.4.2 Hvdrophobin activity on beech wood surface 189
7.4.3 FTIR-spectroscopy 194
7.5 Discussion 195
7.6 References 197
7.7 Appendix 200
8. General discussion: Hydrophobins in wood biology and technology 204
8.1 Introduction 204
8.2 Why to use commune for this project 204
8J S. commune in natural environments, interaction with other
fungi and possible role of hydrophobins in combat interactions 205
8.4 Wood colonization and decay ability of £ commune and
effect of hydrophobias in protein secretion 206
8.5 Application of hydrophobins in wood technology 209
8.6 Some general conclusions from this thesis work regarding the
function of hydrophobias 210
8.7 Future Outlook 211
8.8 References 213
8.9 Appendii to the General discussion:
8.9.1 Effects of deleting the SC3 hydrophobia gene and/or the protein SCI 5
gene from the geaoow of cwmuiiw on growth of the fungus 222
8.9.2 Introduction 222
8.19.1 Methods ami materials 222
Table of contents
8.10.1.1 Fungal strains 222
8.10.1.2 Growth conditions 223
8.10.13 Culture harvest 223
8.10.1.4 Krgosterol measurements 223
8.10.1.4.1 Sample preparation for ergosterol 223
8.10.1.4.2 Quantitative analysis of ergosterol by GC-MS 224
8.10.1.5 2 D Electrophoresis 225
8.10.1.5.1 Precipitation of protein samples from culture
supernatants with TCA 225
8.10.1.5.2. First dimension of 2D-PAGE 226
8.10.2 Results 226
8.10.2.1 Biomass production 226
8.10.2.2 Protein gel electrophoresis 230
8.10 J Further conclusions 230
Curriculum Vitae 232
|
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| spelling | Peddireddi, Sudhakar 1976- Verfasser (DE-588)13875635X aut Hydrophobins in wood biology and biotechnology Sudhakar Peddireddi 2008 XIV, 236 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Göttingen, Univ., Diss., 2009 Hydrophobine (DE-588)4647604-0 gnd rswk-swf Spaltblättling (DE-588)4221680-1 gnd rswk-swf Mikrobieller Abbau (DE-588)4039197-8 gnd rswk-swf Holz (DE-588)4025668-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Spaltblättling (DE-588)4221680-1 s Hydrophobine (DE-588)4647604-0 s Holz (DE-588)4025668-6 s Mikrobieller Abbau (DE-588)4039197-8 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017708273&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Peddireddi, Sudhakar 1976- Hydrophobins in wood biology and biotechnology Hydrophobine (DE-588)4647604-0 gnd Spaltblättling (DE-588)4221680-1 gnd Mikrobieller Abbau (DE-588)4039197-8 gnd Holz (DE-588)4025668-6 gnd |
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| title | Hydrophobins in wood biology and biotechnology |
| title_auth | Hydrophobins in wood biology and biotechnology |
| title_exact_search | Hydrophobins in wood biology and biotechnology |
| title_full | Hydrophobins in wood biology and biotechnology Sudhakar Peddireddi |
| title_fullStr | Hydrophobins in wood biology and biotechnology Sudhakar Peddireddi |
| title_full_unstemmed | Hydrophobins in wood biology and biotechnology Sudhakar Peddireddi |
| title_short | Hydrophobins in wood biology and biotechnology |
| title_sort | hydrophobins in wood biology and biotechnology |
| topic | Hydrophobine (DE-588)4647604-0 gnd Spaltblättling (DE-588)4221680-1 gnd Mikrobieller Abbau (DE-588)4039197-8 gnd Holz (DE-588)4025668-6 gnd |
| topic_facet | Hydrophobine Spaltblättling Mikrobieller Abbau Holz Hochschulschrift |
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