Interaction of Borrelia burgdorferi with human synovial cells: insights into the pathogenesis of Lyme arthritis
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2004
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 107, [61] Bl. Ill., graph. Darst. 30 cm |
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MARC
| LEADER | 00000nam a2200000 c 4500 | ||
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| 001 | BV035571144 | ||
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| 007 | t| | ||
| 008 | 090618s2004 gw ad|| m||| 00||| eng d | ||
| 015 | |a 05,H06,2642 |2 dnb | ||
| 016 | 7 | |a 974496650 |2 DE-101 | |
| 035 | |a (OCoLC)76747032 | ||
| 035 | |a (DE-599)BVBBV035571144 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-12 | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Singh, Sunit Kumar |d 1971- |e Verfasser |0 (DE-588)129926337 |4 aut | |
| 245 | 1 | 0 | |a Interaction of Borrelia burgdorferi with human synovial cells |b insights into the pathogenesis of Lyme arthritis |c vorgelegt von Sunit Kumar Singh |
| 264 | 1 | |c 2004 | |
| 300 | |a 107, [61] Bl. |b Ill., graph. Darst. |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Würzburg, Univ., Diss., 2005 | ||
| 650 | 0 | 7 | |a Lyme-Arthritis |0 (DE-588)4359625-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Borrelia burgdorferi |0 (DE-588)4290954-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Synovialmembran |0 (DE-588)4134251-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Borrelia burgdorferi |0 (DE-588)4290954-5 |D s |
| 689 | 0 | 1 | |a Lyme-Arthritis |0 (DE-588)4359625-3 |D s |
| 689 | 0 | 2 | |a Synovialmembran |0 (DE-588)4134251-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017626725&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-017626725 | |
Record in the Search Index
| _version_ | 1816445526689710080 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
1
SUMMARY
(ZUSAMMENFASSUNG)
1
1.1
ENGLISH
1
1.2
DEUTSCH
6
2
GENERAL
INTRODUCTION
11
2.1
BIOLOGY
OF
BORRELIA
BURGDORFERI
13
2.1.1
OUTER
SURFACE
PROTEINS
OF
B.
BURGDORFERI
15
2.1.2
DECORIN
BINDING
PROTEINS
OF
B.
BURGDORFERI
17
2.1.3
FLAGELLIN
PROTEINS
OFB.
BURGDORFERI
19
3
CLINICAL
FEATURES
OF
LYME
DISEASE
20
3.1
STAGE
I:
EARLY
INFECTION
20
3.2
STAGE
II:
EARLY
DISSEMINATION
21
3.3
STAGE
III:
CHRONIC
DISEASE
22
3.4
DIAGNOSIS
23
3.4.1
MICROBIOLOGICAL
EXAMINATION
METHOD
23
3.4.2
SEROLOGICAL
METHOD
24
3.4.3
DETECTION
BY
POLYMERASE
CHAIN
REACTION
25
4
IMMUNOPATHOGENESIS,
PERSISTENCE
AND
IMMUNE
EVASION
25
4.1
STRATEGIES
OF
ANTIGENIC
VARIATION
AND
IMMUNE
ESCAPE
25
4.1.1
GENETIC
RECOMBINATION
AT
VIS
GENE
LOCUS
26
4.1.2
LATERAL
GENE
TRANSFER
27
4.1.3
LIPOPROTEIN
POLYMORPHISM
28
4.1.4
ROLE
OF
COMPLEMENT
REGULATOR
ACQUIRING
SURFACE
PROTEINS
IN
ESCAPING
THE
HOST
IMMUNE
SYSTEM
29
4.1.5
ROLE
OF
TISSUE
LOCALIZATION
OF
B.
BURGDORFERI
ON
SURVIVAL
AND
IMMUNE
ESCAPE
31
5
INTRODUCTION
TO
SPECIFIC
FIELD
OF
RESEARCH
PRESENTED
33
5.1
CHEMOKINES
35
5.2
ADHESION
MOLECULES
37
5.3
MATRIX
METALLOPROTEINASES
39
5.4
CYCLOOXYGENASES
41
5.5
RECOMBINATION
ACTIVATION
GENES
43
6
MATERIAL
AND
METHODS
USED
IN
CHEMOKINES,
ADHESION
MOLECULES,
MATRIX
METALLOPROTEINASES
AND
CYCLOOXYGENASES
STUDY
46
6.1
CELL
CULTURE
46
6.2
SPIROCHETAL
STRAINS
AND
THEIR
GROWTH
CONDITIONS
46
6.3
COCULTURE
OF
SYNOVIAL
CELLS
WITH
B.
BURGDORFERI
47
6.4
ISOLATION
OF
RNA
AND
SYNTHESIS
OF
FIRST
STRAND
COMPLEMENTARY
CDNA
47
6.5
QUANTIFICATION
OF
CDNA
IN
SAMPLES
48
6.6
POLYMERASE
CHAIN
REACTION
49
6.7
ENZYME
LINKED
IMMUNOSORBANT
ASSAY
(ELISA)
OF
CHEMOKINES
AND
PROSTAGLANDIN
E2
(PGE2)
50
6.8
IMMUNOFLUORESCENCE
STAINING
FOR
SURFACE
EXPRESSION
OF
ICAM-1
51
6.9
STATISTICAL
ANALYSIS
51
7
MATERIAL
AND
METHODS
USED
TO
ASSESS
RAG
EXPRESSION
IN
B
CELLS
EXPOSED
TO
B.
BURGDORFERI
STRAIN
B31
52
7.1
COCULTURE
OF
SYNOVIAL
CELLS
WITH
B.
BURGDORFERI
52
7.2
ADDITION
OF
PBMCS
IN
THE
COCULTURE
SYSTEM
OF
SYNOVIAL
CELLS
ANDS.
BURGDORFERI
52
7.3
IMMUNOFLUORESCENCE
STAINING
53
7.4
SINGLE
CELL
SORTING
AND
PREPARATION
OF
RNA
AND
CDNA
FROM
SORTED
CELLS
53
7.5
PCR
AMPLIFICATION
54
7.6
DETECTION
OF
AMPLIFIED
CDNA
BY
SOUTHERN
BLOT
ANALYSIS
54
7.7
SEQUENCING
OF
GERM-LINE
VK
GENES
55
7.8
STATISTICAL
ANALYSIS
56
8
RESULTS
57
8.1
CHEMOKINE
MRNA
EXPRESSION
IN
HUMAN
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
B.
BURGDORFERI
57
8.2
CHEMOKINE
CONCENTRATION
IN
SUPERNATANT
OF
HUMAN
SYNOVIAL
CELL
CULTURE
AFTER
EXPOSURE
TO
B.
BURGDORFERI
58
8.3
EXPRESSION
OF
ADHESION
MOLECULES
IN
HUMAN
SYNOVIAL
CELLS
AT
MRNA
AND
PROTEIN
LEVELS
AFTER
EXPOSURE
TO
B.
BURGDORFERI
59
8.4
MMP
MRNA
EXPRESSIONS
IN
HUMAN
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
B.
BURGDORFERI
60
8.5
COX
MRNA
EXPRESSION
IN
HUMAN
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
8.
BURGDORFERI
61
8.6
PROSTAGLANDIN
CONCENTRATION
IN
SUPERNATANTS
OF
HUMAN
SYNOVIAL
CELL
CULTURE
AFTER
EXPOSURE
TO
8.
BURGDORFERI
62
8.7
RAG1,
RAG2,
IGG
AND
IGD
MRNA
EXPRESSION
BY
INDIVIDUAL
B
CELLS
ATTACHED
TO
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
8.
BURGDORFERI
62
8.8
VPREB
MRNA
EXPRESSION
BY
INDIVIDUAL
B
CELLS
ATTACHED
TO
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
8.
BURGDORFERI
64
8.9
CD40L
MRNA
EXPRESSION
BY
INDIVIDUAL
B
CELLS
ATTACHED
TO
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
B.
BURGDORFERI
65
8.10
UTILIZATION
OF
VK
AND
JK
GENES
IN
THE
IG
K
LIGHT
CHAINS
65
9
DISCUSSION
67
9.1
CHEMOKINE
EXPRESSION
IN
SYNOVIAL
CELLS
AFTER
EXPOSURE
TOB.
BURGDORFERI
67
9.2
ADHESION
MOLECULES
EXPRESSION
IN
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
B.
BURGDORFERI
72
9.3
MATRIX
METALLOPROTEINASES
AND
CYCLOOXYGENASES
EXPRESSION
IN
SYNOVIAL
CELLS
AFTER
EXPOSURE
TO
8.
BURGDORFERI
75
9.4
RAG
EXPRESSION
IN
PERIPHERAL
B
CELLS
ATTACHED
TO
SYNOVIAL
CELLS
AND
EXPOSED
TO
B.
BURGDORFERI
80
10
REFERENCES
85
11
ABBREVIATIONS
12
APPENDIX,
TABLES
AND
FIGURES
13
FIGURE
LEGENDS
14
CURRICULUM
VITAE
15
LIST
OF
PUBLICATIONS |
| any_adam_object | 1 |
| author | Singh, Sunit Kumar 1971- |
| author_GND | (DE-588)129926337 |
| author_facet | Singh, Sunit Kumar 1971- |
| author_role | aut |
| author_sort | Singh, Sunit Kumar 1971- |
| author_variant | s k s sk sks |
| building | Verbundindex |
| bvnumber | BV035571144 |
| ctrlnum | (OCoLC)76747032 (DE-599)BVBBV035571144 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV035571144 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:44:05Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017626725 |
| oclc_num | 76747032 |
| open_access_boolean | |
| owner | DE-12 |
| owner_facet | DE-12 |
| physical | 107, [61] Bl. Ill., graph. Darst. 30 cm |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| record_format | marc |
| spelling | Singh, Sunit Kumar 1971- Verfasser (DE-588)129926337 aut Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis vorgelegt von Sunit Kumar Singh 2004 107, [61] Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Würzburg, Univ., Diss., 2005 Lyme-Arthritis (DE-588)4359625-3 gnd rswk-swf Borrelia burgdorferi (DE-588)4290954-5 gnd rswk-swf Synovialmembran (DE-588)4134251-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Borrelia burgdorferi (DE-588)4290954-5 s Lyme-Arthritis (DE-588)4359625-3 s Synovialmembran (DE-588)4134251-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017626725&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Singh, Sunit Kumar 1971- Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis Lyme-Arthritis (DE-588)4359625-3 gnd Borrelia burgdorferi (DE-588)4290954-5 gnd Synovialmembran (DE-588)4134251-3 gnd |
| subject_GND | (DE-588)4359625-3 (DE-588)4290954-5 (DE-588)4134251-3 (DE-588)4113937-9 |
| title | Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis |
| title_auth | Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis |
| title_exact_search | Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis |
| title_full | Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis vorgelegt von Sunit Kumar Singh |
| title_fullStr | Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis vorgelegt von Sunit Kumar Singh |
| title_full_unstemmed | Interaction of Borrelia burgdorferi with human synovial cells insights into the pathogenesis of Lyme arthritis vorgelegt von Sunit Kumar Singh |
| title_short | Interaction of Borrelia burgdorferi with human synovial cells |
| title_sort | interaction of borrelia burgdorferi with human synovial cells insights into the pathogenesis of lyme arthritis |
| title_sub | insights into the pathogenesis of Lyme arthritis |
| topic | Lyme-Arthritis (DE-588)4359625-3 gnd Borrelia burgdorferi (DE-588)4290954-5 gnd Synovialmembran (DE-588)4134251-3 gnd |
| topic_facet | Lyme-Arthritis Borrelia burgdorferi Synovialmembran Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017626725&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT singhsunitkumar interactionofborreliaburgdorferiwithhumansynovialcellsinsightsintothepathogenesisoflymearthritis |