Modern biotechnology: connecting innovations in microbiology and biochemistry to engineering fundamentals
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Hoboken, NJ
Wiley
2009
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | Includes index |
| Beschreibung: | XXV, 433 S. Ill., graph. Darst. |
| ISBN: | 9780470114858 |
Internformat
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| 100 | 1 | |a Mosier, Nathan S. |d 1974- |e Verfasser |0 (DE-588)139634916 |4 aut | |
| 245 | 1 | 0 | |a Modern biotechnology |b connecting innovations in microbiology and biochemistry to engineering fundamentals |c Nathan S. Mosier ; Michael R. Ladisch |
| 264 | 1 | |a Hoboken, NJ |b Wiley |c 2009 | |
| 300 | |a XXV, 433 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Includes index | ||
| 650 | 4 | |a Bioréacteurs | |
| 650 | 4 | |a Biotechnologie | |
| 650 | 4 | |a Biotechnologie pharmaceutique | |
| 650 | 4 | |a Enzymes | |
| 650 | 4 | |a Fermentation | |
| 650 | 4 | |a Génie biochimique | |
| 650 | 4 | |a Génie génétique | |
| 650 | 4 | |a Métabolisme | |
| 650 | 4 | |a Biotechnology | |
| 650 | 0 | 7 | |a Biotechnologie |0 (DE-588)4069491-4 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Biotechnologie |0 (DE-588)4069491-4 |D s |
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Datensatz im Suchindex
| _version_ | 1804138868976386048 |
|---|---|
| adam_text | CONTENTS
Preface
xv
Acknowledgments
xvii
List of Illustrations
xix
1
Biotechnology
1
Introduction
1
The Directed Manipulation of Genes Distinguishes the New
Biotechnology from Prior Biotechnology
2
Growth of the New Biotechnology Industry Depends on
Venture Capital
3
Submerged Fermentations Are the Industry s
Bioprocessing
Cornerstone
10
Oil Prices Affect Parts of the Fermentation Industry
10
Growth of the Antibiotic/Pharmaceutical Industry
11
The Existence of Antibiotics Was Recognized in
1877 11
Penicillin Was the First Antibiotic Suitable for Human
Systemic Use
12
Genesis of the Antibiotic Industry
12
Other Antibiotics Were Quickly Discovered after the
Introduction of Penicillin
13
Discovery and Scaleup Are Synergistic in the Development of
Pharmaceutical Products
15
Success of the Pharmaceutical Industry in Research, Development,
and Engineering Contributed to Rapid Growth but Also
Resulted in Challenges
15
Growth of the
Amino Acid/Acidulant
Fermentation Industry
16
Production of
Monosodium Glutamate
(MSG) via Fermentation
17
The Impact of Glutamic Acid Bacteria on
Monosodium
Glutamate
Cost Was Dramatic
17
Auxotrophic and Regulatory Mutants Enabled Production of
Other
Amino
Acids
17
Prices and Volumes Are Inversely Related
19
Biochemical Engineers Have a Key Function in All Aspects of
the Development Process for Microbial Fermentation
21
VÍ
CONTENTS
References
22
Homework Problems
24
2
New Biotechnology
27
Introduction
27
Growth of the Biopharmaceutical Industry
28
The Biopharmaceutical Industry Is in the Early Part of Its
Life Cycle
31
Discovery of Type II Restriction Endonucleases Opened a New
Era in Biotechnology
33
The Polymerase Chain Reaction (PCR) Is an Enzyme-Mediated,
In Vitro Amplification of
DNA 33
Impacts of the New Biotechnology on Biopharmaceuticals, Genomics,
Plant Biotechnology, and Bioproducts
34
Biotechnology Developments Have Accelerated Biological
Research
35
Drug Discovery Has Benefited from Biotechnology Research
Tools
36
The Fusing of Mouse Spleen Cells with
Τ
Cells Facilitated
Production of Antibodies
36
Regulatory Issues Add to the Time Required to Bring a New
Product to Market
36
New Biotechnology Methods Enable Rapid Identification of
Genes and Their Protein Products
39
Genomics Is the Scientific Discipline of Mapping, Sequencing, and
Analyzing Genomes
39
Products from the New Plant Biotechnology Are Changing the
Structure of Large Companies that Sell Agricultural Chemicals
42
Bioproducts from Genetically Engineered Microorganisms
Will Become Economically Important to the
Fermentation Industry
43
References
45
Homework Problems
47
3
Bioproducts and Biofuels
49
Introduction
49
Biocatalysis and the Growth of Industrial Enzymes
49
Glucose Isomerase Catalyzed the Birth of a New Process for
Sugar Production from Corn
51
Identification of a Thermally Stable Glucose Isomerase and an
Inexpensive Inducer Was Needed for an Industrial Process
53
The Demand for High-Fructose Corn Syrup (HFCS) Resulted
in Large-Scale Use of Immobilized Enzymes and Liquid
Chromatography
53
CONTENTS
VU
Rapid
Growth of HFCS Market Share Was Enabled by Large-
Scale Liquid Chromatography and Propelled by Record-High
Sugar Prices
55
Biocatalysts Are Used in Fine-Chemical Manufacture
56
Growth of Renewable Resources as a Source of Specialty Products
and Industrial Chemicals
58
A Wide Range of Technologies Are Needed to Reduce Costs
for Converting Cellulosic Substrates to Value-Added
Bioproducts and Biofuels
59
Renewable Resources Are a Source of Natural Plant Chemicals
63
Bioseparations Are
Important to the Extraction, Recovery, and
Purification of Plant-Derived Products
64
Bioprocess
Engineering and Economics
65
Bioseparations
and
Bioprocess
Engineering
66
References
67
Homework Problems
71
4
Microbial Fermentations
73
Introduction
73
Fermentation Methods
75
Fermentations Are Carried Out in Flasks, Glass Vessels,
and Specially Designed Stainless-Steel Tanks
75
Microbial Culture Composition and Classification
78
Microbial Cells: Prokaryotes versus Eukaryotes
78
Classification of Microorganisms Are Based on Kingdoms
81
Prokaryotes Are Important Industrial Microorganisms
81
Eukaryotes Are Used Industrially to Produce
Ethanol,
Antibiotics, and Biotherapeutic Proteins
82
Wild-Type Organisms and Growth Requirements in
Microbial Culture
83
Wild-Type Organisms Find Broad Industrial Use
83
Microbial Culture Requires that Energy and All Components
Needed for Cell Growth Be Provided
86
Media Components and Their Functions (Complex and
Defined Media)
86
Carbon Sources Provide Energy, and Sometimes
Provide Oxygen
86
Complex Media Have a Known Basic Composition but a
Chemical Composition that Is Not Completely Defined
89
Industrial Fermentation Broths May Have a High Initial Carbon
(Sugar) Content
(Ethanol
Fermentation Example)
91
The Accumulation of Fermentation Products Is Proportional to
Cell Mass in the Bioreactor
92
VIU
CONTENTS
A Microbial Fermentation Is Characterized by Distinct Phases
of Growth
93
Expressions for Cell Growth Rate Are Based on Doubling
Time
94
Products of Microbial Culture Are Classified According to
Their Energy Metabolism (Types I, II, and III Fermentations)
96
Product Yields Are Calculated from the Stoichiometry of
Biological Reactions (Yield Coefficients)
102
The Embden-Meyerhof Glycolysis and Citric Acid Cycles Are
Regulated by the Relative Balance of ATP, ADP, and AMP
in the Cell
104
References
105
Homework Problems
108
5
Modeling and Simulation 111
Introduction 111
The Runge-Kutta Method
112
Simpson s Rule
112
Fourth-Order Runge-Kutta Method
113
Ordinary Differential Equations (ODEs)
115
Runge-Kutta Technique Requires that Higher-Order Equations
Be Reduced to First-Order ODEs to Obtain Their Solution
115
Systems of First-Order ODEs Are Represented in Vector Form
116
Kinetics of Cell Growth
117
Ks Represents Substrate Concentration at Which the Specific
Growth Rate Is Half Its Maximum
120
Simulation of a Batch
Ethanol
Fermentation
122
Ethanol
Case Study
123
Luedeking-Piret Model
127
Continuous Stirred-Tank Bioreactor
128
Batch Fermentor versus Chemostat
132
References
133
Homework Problems
135
6
Aerobic Bioreactors
141
Introduction
141
Fermentation Process
144
Fermentation of Xylose to 2,3-Butanediol by Klebsiella
oxytoca Is Aerated but Oxygen-Limited
144
Oxygen Transfer from Air Bubble to Liquid Is Controlled by
Liquid-Side Mass Transfer
153
CONTENTS
ІХ
Chapter
6 Appendix: Excel
Program for
Integration
of
Simultaneous
Differential
Equations
159
References
161
Homework Problems
162
7
Enzymes
165
Introduction
165
Enzymes and Systems Biology
165
Industrial Enzymes
166
Enzymes: In Vivo and In Vitro
167
Fundamental Properties of Enzymes
169
Classification of Enzymes
170
Sales and Applications of Immobilized Enzymes
172
Assaying Enzymatic Activity
173
Enzyme Assays
181
Batch Reactions
187
Thermal Enzyme Deactivation
187
References
192
Homework Problems
195
8
Enzyme Kinetics
199
Introduction
199
Initial Rate versus Integrated Rate Equations
200
Obtaining Constants from Initial Rate Data Is an Iterative
Process
204
Batch Enzyme Reactions: Irreversible Product Formation
(No Inhibition)
207
Rapid Equilibrium Approach Enables Rapid Formulation of
an Enzyme Kinetic Equation
207
The Pseudo-Steady-State Method Requires More Effort to Obtain
the Hart Equation but Is Necessary for Reversible Reactions
209
Irreversible Product Formation in the Presence of Inhibitors
and Activators
210
Inhibition
212
Competitive Inhibition
213
Uncompetitive Inhibition
214
(Classical)
Noncompetitive
Inhibition
216
Substrate Inhibition
217
CONTENTS
Example of Reversible Reactions
220
Coenzymes and Cofactors Interact in a Reversible Manner
223
King-Altman Method
225
Immobilized Enzyme
234
Online Databases of Enzyme Kinetic Constants
236
References
237
Homework Problems
238
9
Metabolism
243
Introduction
243
Aerobic and Anaerobic Metabolism
245
Glycolysis Is the Oxidation of Glucose in the Absence of Oxygen
245
Oxidation Is Catalyzed by
Oxidases
in the Presence of O2,
and by Dehydrogenases in the Absence of O2
246
A Membrane Bioreactor Couples Reduction and Oxidation
Reactions (R-Mandelic Acid Example)
247
Three Stages of Catabolism Generate Energy, Intermediate
Molecules, and Waste Products
248
The Glycolysis Pathway Utilizes Glucose in Both Presence
(Aerobic) and Absence (Anaerobic) of O2 to Produce Pyruvate
249
Glycolysis Is Initiated by Transfer of a High-Energy Phosphate
Group to Glucose
250
Products of Anaerobic Metabolism Are Secreted or Processed
by Cells to Allow Continuous Metabolism of Glucose by
Glycolysis
253
Other Metabolic Pathways Utilize Glucose Under Anaerobic
Conditions (Pentose Phosphate, Entner-Doudoroff, and
Hexose
Monophosphate
Shunt Pathways)
255
Knowledge of Anaerobic Metabolism Enables Calculation of
Theoretical Yields of Products Derived from Glucose
257
Economics Favor the Glycolytic Pathway for Obtaining
Oxygenated Chemicals from Renewable Resources
258
Citric Acid Cycle and Aerobic Metabolism
259
Respiration Is the Aerobic Oxidation of Glucose and Other
Carbon-Based Food Sources (Citric Acid Cycle)
260
The Availability of Oxygen, under Aerobic Conditions,
Enables Microorganisms to Utilize Pyruvate via the Citric
Acid Cycle
260
The Citric Acid Cycle Generates Precursors for Biosynthesis of
Amino
Acids and Commercially Important Fermentation
Products
264
Glucose Is Transformed to Commercially Valuable Products via
Fermentation Processes: A Summary
264
Essential
Amino
Acids Not Synthesized by Microorganisms
Must Be Provided as Nutrients (Auxotrophs)
267
CONTENTS
ХІ
The Utilization of Fats in Animals Occurs by a Non-
Tricarboxylic Acid (TCA) Cycle Mechanism
267
Some Bacteria and Molds Can Grow on Hydrocarbons or
Methanol in
Aerated Fermentations (Single-Cell Protein
Case Study)
269
Extremophiles: Microorganisms that Do Not Require Glucose,
Utilize H2, and Grow at
80-100 °С
and
200
atm Have
Industrial Uses
270
The Terminology for Microbial Culture Is Inexact: Fermentation
Refers to Both Aerobic and Anaerobic Conditions While
Respiration Can Denote Anaerobic Metabolism
271
Metabolism and Biological Energetics
272
References
272
Homework Problems
273
10
Biological Energetics
277
Introduction
277
Redox
Potential and Gibbs Free Energy in Biochemical Reactions
277
Heat: Byproduct of Metabolism
286
References
292
Homework Problems
293
11
Metabolic Pathways
295
Introduction
295
Living Organisms Control Metabolic Pathways at Strategic and
Operational Levels
296
Auxotrophs Are Nutritionally Deficient Microorganisms that
Enhance Product Yields in Controlled Fermentations (Relief
of Feedback Inhibition and Depression)
296
Both Branched and Unbranched Pathways Cause Feedback
Inhibition and Repression
(Purine Nucleotide
Example)
299
The Accumulation of an End Metabolite in a Branched Pathway
Requires a Strategy Different from that for the Accumulation
of an Intermediate Metabolite
301
Amino
Acids
305
The Formulation of Animal Feed Rations with Exogeneous
Amino
Acids Is a Major Market for
Amino
Acids
306
Microbial Strain Discovery, Mutation, Screening, and
Development Facilitated Introduction of Industrial, Aerated
Fermentations for
Amino
Acid Production by Corynbacterium
glutamicutn
308
Overproduction of
Glutamate
by
С
glutamicum Depends
on an Increase in Bacterial Membrane Permeability
(Biotin-Deficient Mutant)
309
Xli
CONTENTS
A Threonine and Methionine Auxotroph
of C.
glutamicum
Avoids
Concerted
Feedback Inhibition
and Enables Industrial
Lysine
Fermentations
310
Cell (Protoplast)
Fusion
Is a Method for Breeding
Amino
Acid
Producers that Incorporate Superior Characteristics of Each
Parent
(Lysine
Fermentation)
312
Amino
Acid Fermentations Represent Mature Technologies
313
Antibiotics
314
Secondary Metabolites Formed During Idiophase Are Subject
to
Cata
bolite
Repression and Feedback Regulation
(Penicillin and Streptomycin)
314
The Production of Antibiotics Was Viewed as a Mature
Field Until Antibiotic-Resistant Bacteria Began to Appear
317
Bacteria Retain Antibiotic Resistance Even When
Use of the Antibiotic Has Ceased for Thousands
of Generations
318
Antibiotic Resistance Involves Many Genes
(Vancomycin Example)
318
References
320
Homework Problems
323
12
Genetic Engineering:
DNA, RNA,
and Genes
331
Introduction
331
DNA
and
RNA
332
DNA
Is a Double-Stranded Polymer of the Nucleotides:
Thymine,
Adenine, Cytosine,
and Guanine
332
The Information Contained in
DNA
Is Huge
332
Genes Are Nucleotide Sequences that Contain the Information
Required for the Cell to Make Proteins
333
Transcription Is a Process Whereby Specific Regions of the
DNA
(Genes) Serve as a Template to Synthesize Another
Nucleotide, Ribonucleic Acid
(RNA)
333
Chromosomal
DNA
in a Prokaryote (Bacterium) Is
Anchored to the Cell s Membrane While Plasmids Are in
the Cytoplasm
333
Chromosomal
DNA in
a Eukaryote (Yeast, Animal or Plant
Cells) Is Contained in the Nucleus
334
Microorganisms Carry Genes in Plasmids Consisting of Shorter
Lengths of Circular,
Extrachromosomal DNA 334
Restriction Enzymes Enable Directed In Vitro Cleavage of
DNA 337
Different Type II Restriction Enzymes Give Different Patterns
of Cleavage and Different Single-Stranded Terminal
Sequences
339
DNA
Ligase Covalently
Joins the Ends of
DNA
Fragments
341
CONTENTS
ХІІІ
DNA Fragments and Genes
of <150 Nucleotides Can Be
Chemically Synthesized if the Nucleotide Sequence Has Been
Predetermined
342
Protein Sequences Can Be Deduced and Genes Synthesized
on the Basis of Complementary
DNA
Obtained from
Messenger
RNA
343
Genes and Proteins
344
Selectable Markers Are Genes that Facilitate Identification of
Transformed Cells that Contain
Recombinant
DNA 344
A Second Protein Fused to the Protein Product Is Needed to
Protect the Product from Proteolysis
(ß-Gal-Somatostatin
Fusion Protein Example)
346
Recovery of Protein Product from Fusion Protein Requires
Correct Selection of
Amino
Acid that Links the Two Proteins
(Met Linker)
347
Chemical Modification and Enzyme Hydrolysis Recover an
Active Molecule Containing Met Residues from a Fusion
Protein
(ß-Endorphin
Example)
347
Metabolic Engineering Differs from Genetic Engineering by
the Nature of the End Product
348
References
349
Homework Problems
350
13
Metabolic Engineering
355
Introduction
355
Building Blocks
359
L-Threonine-Overproducing Strains of
E. coli K-12
359
Genetically Altered Brevibacterium lactoferrin Has Yielded
Improved
Amino
Acid-Producing Strains
360
Metabolic Engineering May Catalyze Development of New
Processes for Manufacture of Oxygenated Chemicals
362
Gene Chips Enable Examination of Glycolytic and Citric
Acid Cycle Pathways in Yeast at a Genomic Level
(Yeast Genome Microarray Case Study)
362
The Fermentation of Pentoses to
Ethanol
Is a Goal of
Metabolic Engineering
(Recombinant
Bacteria and
Yeast Examples)
364
Metabolic Engineering for a l^-Propanediol-Producing
Organism to Obtain Monomer for Polyester Manufacture
370
Redirection of Cellular Metabolism to Overproduce an
Enzyme Catalyst Results in an Industrial Process for
Acrylamide Production (Yamada-Nitto Process)
373
References
377
Homework Problems
379
XIV CONTENTS
14
Genomes and Genomics
385
Introduction
385
Human Genome Project
385
Deriving Commercial Potential from Information Contained
in Genomes
388
The Genome for
E. coli
Consists of
4288
Genes that Code
for Proteins
390
DNA
Sequencing Is Based on Electrophoretic Separations
of Defined
DNA
Fragments
391
Sequence-Tagged Sites (STSs) Determined from
Complementary
DNA (cDNA)
Give Locations of Genes
394
Single-Nucleotide Polymorphisms (SNPs) Are Stable Mutations
Distributed throughout the Genome that Locate Genes More
Efficiently than Do STSs
394
Gene Chip Probe Array
398
Polymerase Chain Reaction (PCR)
401
The Polymerase Chain Reaction Enables
DNA
to Be
Copied In Vitro
402
Thermally Tolerant
DNA
Polymerase from Tbermus
aquaticus Facilitates Automation of PCR
403
Only the
5
-Terminal Primer Sequence Is Needed to Amplify
the
DNA
by PCR
404
The Sensitivity of PCR Can Be a Source of Significant
Experimental Error
405
Applications of PCR Range from Obtaining Fragments of
Human
DNA
for Sequencing to Detecting Genes Associated
with Diseases
405
Conclusions
406
References
407
Homework Problems
409
Index
411
A unique resource for the
% next generation of biotech innovators
Enabling everything from the deciphering of the human genome to envi¬
ronmentally friendly biofuels to lifesaving new Pharmaceuticals, biotech¬
nology has blossomed as an area of discovery and opportunity. Modern
Biotechnology provides a much-needed introduction connecting the latest
innovations in this area to key engineering fundamentals. With an unmatched
í
level of coverage, this unique resource prepares a wide range of readers for the practi-
Ical application of biotechnology in biopharmaceuticals, biofuels, and other bioproducts.
¡Organized into fourteen sections, reflecting a typical semester course, Modern Biotechnology
(¿overs such key topics as:
•
Metabolic engineering
•
Enzymes and enzyme kinetics
•
Biocatalysts and other new bioproducts
•
Cell fusion
•
Genetic engineering,
DNA, RNA,
and genes
•
Genomes and genomics
;
•
Production of biopharmaceuticals
•
Fermentation modeling and process analysis
Taking a practical, applications-based approach, the text presents discussions of important
fundamentals in biology, biochemistry, and engineering with relevant case studies showing
technology applications and manufacturing scale-up. Written for today s wider, more interdis¬
ciplinary readership, Modern Biotechnology offers a solid intellectual foundation for students
and professionals entering the modem biotechnology industry.
NATHAN S.
MOSIER
is an Associate Professor in the Department of Agricultural and
Biological Engineering and the Laboratory of Renewable Resources Engineering at Purdue
University.
Mosier
was also an NSF IGERT PhD Fellow from
2000-2002
at the Innovation
Realization Laboratory in Krannert School of Management, and has authored case studies
based on commercialization experiences for use in
entrepreneurship
and/or technology com¬
mercialization business school curricula.
MICHAEL R. LADISCH, a member of the National Academy of Engineering, is Distinguished
Professor in the Department of Agricultural and Biological Engineering and the
Weldon
School
of
Biomedical
Engineering, Director of the Laboratory of Renewable Resources Engineering
at Purdue University, and Chief Technology Officer at Mascoma
Corporation, a cellulosici
biofuels company. He is
coeditor
of several books on biotechnology, including Harnessing
Biotechnology for the 21st Century and Protein Purification, and is the author of Wiley s
Bioseparations
Engineering text.
|
| any_adam_object | 1 |
| author | Mosier, Nathan S. 1974- |
| author_GND | (DE-588)139634916 (DE-588)135734053 |
| author_facet | Mosier, Nathan S. 1974- |
| author_role | aut |
| author_sort | Mosier, Nathan S. 1974- |
| author_variant | n s m ns nsm |
| building | Verbundindex |
| bvnumber | BV035428567 |
| callnumber-first | T - Technology |
| callnumber-label | TP248 |
| callnumber-raw | TP248.2 |
| callnumber-search | TP248.2 |
| callnumber-sort | TP 3248.2 |
| callnumber-subject | TP - Chemical Technology |
| classification_rvk | WF 9700 WF 9720 |
| classification_tum | CIT 900f CHE 800f BIO 250f |
| ctrlnum | (OCoLC)255894331 (DE-599)BVBBV035428567 |
| dewey-full | 660.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6 |
| dewey-search | 660.6 |
| dewey-sort | 3660.6 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie Chemie Chemie-Ingenieurwesen Biotechnologie |
| format | Book |
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| id | DE-604.BV035428567 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:35:02Z |
| institution | BVB |
| isbn | 9780470114858 |
| language | English |
| lccn | 2009001779 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017348997 |
| oclc_num | 255894331 |
| open_access_boolean | |
| owner | DE-703 DE-91G DE-BY-TUM DE-526 DE-91S DE-BY-TUM DE-83 DE-19 DE-BY-UBM DE-11 DE-634 DE-188 DE-1102 |
| owner_facet | DE-703 DE-91G DE-BY-TUM DE-526 DE-91S DE-BY-TUM DE-83 DE-19 DE-BY-UBM DE-11 DE-634 DE-188 DE-1102 |
| physical | XXV, 433 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Wiley |
| record_format | marc |
| spelling | Mosier, Nathan S. 1974- Verfasser (DE-588)139634916 aut Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals Nathan S. Mosier ; Michael R. Ladisch Hoboken, NJ Wiley 2009 XXV, 433 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Includes index Bioréacteurs Biotechnologie Biotechnologie pharmaceutique Enzymes Fermentation Génie biochimique Génie génétique Métabolisme Biotechnology Biotechnologie (DE-588)4069491-4 gnd rswk-swf Biotechnologie (DE-588)4069491-4 s DE-604 Ladisch, Michael R. 1950- Sonstige (DE-588)135734053 oth Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348997&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348997&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Mosier, Nathan S. 1974- Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals Bioréacteurs Biotechnologie Biotechnologie pharmaceutique Enzymes Fermentation Génie biochimique Génie génétique Métabolisme Biotechnology Biotechnologie (DE-588)4069491-4 gnd |
| subject_GND | (DE-588)4069491-4 |
| title | Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals |
| title_auth | Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals |
| title_exact_search | Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals |
| title_full | Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals Nathan S. Mosier ; Michael R. Ladisch |
| title_fullStr | Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals Nathan S. Mosier ; Michael R. Ladisch |
| title_full_unstemmed | Modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals Nathan S. Mosier ; Michael R. Ladisch |
| title_short | Modern biotechnology |
| title_sort | modern biotechnology connecting innovations in microbiology and biochemistry to engineering fundamentals |
| title_sub | connecting innovations in microbiology and biochemistry to engineering fundamentals |
| topic | Bioréacteurs Biotechnologie Biotechnologie pharmaceutique Enzymes Fermentation Génie biochimique Génie génétique Métabolisme Biotechnology Biotechnologie (DE-588)4069491-4 gnd |
| topic_facet | Bioréacteurs Biotechnologie Biotechnologie pharmaceutique Enzymes Fermentation Génie biochimique Génie génétique Métabolisme Biotechnology |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348997&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348997&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
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