Autophagy: C Autophagy in disease and clinical applications
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier
2009
|
| Schriftenreihe: | Methods in enzymology
453 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLIX, 481 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780123749369 |
Internformat
MARC
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|---|---|---|---|
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| 003 | DE-604 | ||
| 005 | 20110803 | ||
| 007 | t | ||
| 008 | 090408s2009 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123749369 |9 978-0-12-374936-9 | ||
| 035 | |a (OCoLC)645662393 | ||
| 035 | |a (DE-599)BVBBV035427843 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-19 |a DE-12 |a DE-91G |a DE-20 |a DE-355 | ||
| 245 | 1 | 0 | |a Autophagy |n C |p Autophagy in disease and clinical applications |c ed. by Daniel J. Klionsky |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier |c 2009 | |
| 300 | |a XLIX, 481 S., [8] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 453 | |
| 490 | 0 | |a Methods in enzymology |v ... | |
| 700 | 1 | |a Klionsky, Daniel J. |e Sonstige |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV035270787 |g 3 |
| 830 | 0 | |a Methods in enzymology |v 453 |w (DE-604)BV000000938 |9 453 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348280&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-017348280 | ||
Datensatz im Suchindex
| _version_ | 1804138867731726336 |
|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xxi
Volumes in Series
xxiii
1.
Initiation of Autophagy by Photodynamic Therapy
1
David
Kessel
and Nancy L. Oleinick
1.
Introduction
2
2.
Photosensitizing Agents
3
3.
Additional Factors Unique to
PDT
5
4.
A Typical
PDT
Protocol
5
5.
Identification and Characterization of Autophagy after
PDT
6
6.
Effects of Autophagy on
PDT
Responses
9
Acknowledgments
15
References
15
2.
Autophagic Cell Death
17
Michael J. Lenardo, Christina K. McPhee, and Li Yu
1.
Introduction
18
2.
Methods to Quantify Cell Death
19
3.
Methods to Measure Autophagy
26
4.
Methods to Establish Autophagy as the Cause of Cell Death
27
5.
Autophagy Genes for RNAi Silencing
29
6.
Transfect RNAi by Electroporation (Amaxa Nucleofection)
29
7.
Conclusion
29
References
30
3.
Autophagic Neuron Death
33
Yasuo Uchiyama, Masato Koike, Masahiro Shibata, and Mitsuho Sasaki
1.
Introduction
34
2.
Experimental Models of Neurodegeneration
35
3.
Measurements of Neuron Death
41
References
48
Contents
4.
Assessing Metabolic Stress and Autophagy
Status in Epithelial Tumors
53
Robin Mathew, Vassiliki Karantza-Wadsworth, and Eileen White
1.
Introduction
55
2.
Mouse Epithelial Cell Models for Studying the Role of
Autophagy in Cancer
57
3.
Protocols for Monitoring Autophagy in ¡BMK Cells and iMMECs In Vitro
61
4.
Protocols for Monitoring Autophagy in Tumors In Vivo
64
5.
Monitoring Chromosomal Instability
Oue
to Autophagy Defects
67
6.
Concluding Remarks and Future Perspectives
78
References
79
5.
Autophagic Clearance of Aggregate-Prone Proteins Associated
with Neurodegeneration
83
Sovan
Särkar,
Brinda
Ravikumar, and David C. Rubinsztein
1.
Introduction
84
2.
Aggregate-Prone Intracytoplasmic Proteins Associated with
Neurodegenerative
Disorders are Autophagy Substrates
86
3.
Assays for the Clearance of Aggregate-Prone Proteins
87
4.
Measurement of Autophagic Flux Using Bafilomycin
Ai
102
5.
Concluding Remarks
107
Acknowledgments
107
References
107
6.
Monitoring Autophagy in Alzheimer s Disease and Related
Neurodegenerative
Diseases 111
Dun-Sheng Yang, Ju-Hyun Lee, and Ralph A. Nixon
1.
Introduction
112
2.
General Approaches to Investigations of Human Neurodegeneration
113
3.
Characterization of Autophagic
Vacuoles, Evaluation
of
Autophagosome and Autolysosome Formation, and
Autolysosomal Clearance
119
4.
Metabolic Analyses of Autophagy in Neuronat and
Nonneuronat Celt Models
132
5.
Isolation and Characterization of Autophagic
Vacuoles
and
Lysosomes from Cell Cultures and Brain Tissue
135
6.
Western Blot Analysis of Autophagy Components and Substrates
136
References
240
Contents
vii
7.
Live-Cell Imaging of Autophagy Induction and
Autophagosome-Lysosome Fusion in Primary Cultured Neurons
145
Mona
Bains and Kim A.
Heidenreich
1.
Introduction
146
2.
Cultured Cerebellar Purkinje Neurons as a Model to Study
Neuronal
Autophagy
148
3.
Characterization of Autophagic
Vacuole
Size and Number in
Purkinje Neurons
151
4.
Using Colocalization of Fluorescent Tags to Measure
Autophagosome-Lysosome Fusion
154
5.
Concluding Remarks
156
Acknowledgments
157
References
157
8.
Using Genetic Mouse Models to Study
the Biology and Pathology of Autophagy in the
Central Nervous System
159
Zhenyu Yue, Gay R.
Holstein,
Brian T. Chait, and Qing
jun Wang
1.
Introduction
160
2.
Methods
164
3.
Analysis of GFP-LCS Expression and Subcellular
Localization in the CNS
167
4.
Analysis of
рбг
/SQSTMl
and Ubiquitinated Protein
Inclusions in the CNS
171
5.
Transmission Electron Microscopy
(ГЕМ)
Analysis of
Autophagosomes
173
6.
Conclusion
177
Acknowledgments
178
References
178
9.
Biochemical and Morphological Detection
of Inclusion Bodies in Autophagy-Deficient Mice
181
Satoshi Waguri and Masaaki Komatsu
1.
Introduction
182
2.
Detection of Ubiquitinated Proteins and p62 in
Autophagy-Deficient Mice by Western Blot Analysis
183
3.
Detection of Ubiquitinated Proteins and p62 in Cultured
Hepatocytes Derived from Autophagy-Deficient Mice
187
v¡¡¡
Contents
4.
Detection of Ubiquitin- and
рбг-Роѕ^ме
Inclusions at the
Light Microscopy Level
188
5.
Detection of Ubiquitin- and p62-Positive Inclusions at the
Electron Microscopy Level
192
6.
Conclusion
194
Acknowledgments
195
References
195
10.
Analyzing Autophagy in Clinical Tissues of Lung and
Vascular Diseases
197
Hong Pyo Kim, Zhi-Hua Chen, Augustine M. K. Choi, and Stefan W.
Ryter
1.
Introduction
198
2.
Methods for Preparation of Lung and Vascular Cells
199
3.
Analysis of Autophagy
205
4.
Chromatin Immunoprecipitation
211
5.
Conclusions
214
References
215
11.
Autophagy in Neurite Injury and Neurodegeneration:
In Vitro and In Vivo Models
217
Charleen
T. Chu,
Edward
D.
Plowey, Ruben
K. Dagda,
Robert W. Hickey,
Salvatore
J.
Cherra
III, and Robert
S. B.
Clark
1.
Introduction
218
2.
Studying
Neuronal
Autophagy In Vitro
220
3.
Studying Brain Autophagy In Vivo
238
4.
Future Perspectives and Challenges
244
Acknowledgments
244
References
244
12.
Monitoring the Autophagy Pathway in Cancer
251
Frank
С
Dorsey, Meredith A. Steeves, Stephanie M. Prater,
Thomas
Schröter,
and John L. Cleveland
1.
Introduction
252
2.
LC3: A Phenotypic and Functional Marker of Autophagy
254
3.
Assessing the Role of Autophagy
in
E^-Myc-Driven Lymphoma 264
4.
Concluding Remarks and Future Perspectives
269
Acknowledgments
269
References
269
Contents
¡χ
13.
Autophagy Pathways in Glioblastoma
273
Hong Jiang, Erin J. White, Charles Conrad,
Candelaria Gómez-Manzano,
and Juan Fueyo
1.
Introduction: Autophagy and Gliomas
274
2.
Prioritization of Methods to Characterize Autophagy in Gliomas
275
3.
In Vitro Cellular Markers
276
4.
In Vitro Biochemical Markers
279
5.
Electron Microscopy to Monitor the Autophagic
Vacuoles
281
6.
In Vivo Analysis of Biochemical Markers
281
7.
Autophagy Indicators as Surrogate Markers of
Treatment Effect in Clinical Trials
284
8.
Future Directions
284
References
285
14.
Autophagy in Lung Cancer
287
Jerry J. Jaboin, Misun Hwang, and Bo
Lu
1.
Introduction
288
2.
Methods
291
3.
Conclusion
301
References
301
15.
Signal-Dependent Control of Autophagy-Related
Gene Expression
305
Fulvio Chiacchiera
and
Cristiano
Simone
1.
Introduction
306
2.
Overview: Signal Transduction and Chromatin-Associated Kinases
307
3.
The p38 Pathway in Colorectal Cancer Cells
308
4.
Methods to Test Kinase Activity
309
5.
Profiling Gene Expression Pattern
311
6.
Transcriptional Control of ATG Genes
312
7.
Analysis of Transcriptional Multiprotein Complexes
318
8.
Concluding Remarks
322
Acknowledgments
323
References
323
16.
Novel Methods for Measuring Cardiac Autophagy In Vivo
325
Cynthia
N.
Perry, Shiori Kyoi, Nirmala Hariharan, Hiromitsu Takagi,
Junichi Sadoshima, and Roberta A. Gottlieb
1.
Introduction
326
2.
In Vivo Models of Autophagy in the Myocardium
329
x
Contents
3.
Discussion
339
Acknowledgments
341
References
341
17.
Autophagy in Load-Induced Heart Disease
343
Hongxin Zhu, Beverly A. Rothermel, and Joseph A. Hill
1.
Introduction
344
2.
Mouse Models of Load-Induced Heart Disease
345
3.
Analysis of Ventricular Remodeling
346
4.
In Vitro Models of Load-Induced Hypertrophy
349
5.
Techniques to Analyze Cardiomyocyte Autophagy
350
6.
Immunohistochemistry for LAMP-1 or Cathepsin
D
to Monitor
Changes in Lysosomal Abundance
352
7.
Isolation of LC3 Proteins from NRVM in Culture
356
8.
Isolation of LC3 Protein from Heart or Skeletal Muscle Tissue
357
9.
Soluble/Insoluble Fractionation of NRVM
359
10.
Perspective
360
Acknowledgments
360
References
360
18.
Evaluation of Cell Death Markers in Severe
Calcified Aortic Valves
365
Wilhelm Mistiaen
and Michiel Knaapen
1.
Clinical Importance of Degenerative Aortic Valve Disease
366
2.
Pathological Appearances of
AVD
366
3.
Mechanism of Progression of
AVD
367
4.
Autophagy: Major Player in the Progression of Aortic Valve Disease?
368
5.
Methods for Detection of Cell Death Markers in the
Degenerated Aortic Valve
370
6.
Methods for Quantification of Calcified Aortic Valves
374
7.
Discussion
376
8.
Prospects and Concluding Remarks
376
References
377
19.
Monitoring Autophagy in Muscle Diseases
379
May Christine V. Malicdan,
Šatoru
Noguchi, and Ichizo Nishino
1.
Introduction
380
2.
Histological Observation of Skeletal Muscles
381
3.
Measuring Autophagy by Protein Quantification
387
4.
Monitoring Autophagy in Cultured Skeletal Myocytes
388
Contents xi
5. Electron
Microscopy Observation of Skeletal Muscles
392
6.
Immunoelectron Microscopy
394
7.
Conclusion
395
References
395
20.
Analyzing Macroautophagy in Hepatocytes and the Liver
397
Wen-Xing Ding and Xiao-Ming Yin
1.
The Pathophysiological Relevance of Macroautophagy in the Liver
398
2.
Analysis of Autophagy in Isolated Hepatocytes
398
3.
Analysis of Autophagy in the Liver
410
4.
Summary
413
Acknowledgments
414
References
414
21.
Monitoring Autophagy in Lysosomal Storage Disorders
417
Nina
Raben,
Lauren Shea, Victoria Hill, and Paul Plotz
1.
Introduction
418
2.
General Techniques to Monitor Autophagy In LSDs
419
3.
LSDs Analyzed for Autophagic Involvement
428
4.
Conclusion
444
References
445
Author Index
451
Subject Index
471
|
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| id | DE-604.BV035427843 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:35:01Z |
| institution | BVB |
| isbn | 9780123749369 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017348280 |
| oclc_num | 645662393 |
| open_access_boolean | |
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| owner_facet | DE-19 DE-BY-UBM DE-12 DE-91G DE-BY-TUM DE-20 DE-355 DE-BY-UBR |
| physical | XLIX, 481 S., [8] Bl. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Autophagy C Autophagy in disease and clinical applications ed. by Daniel J. Klionsky Amsterdam [u.a.] Elsevier 2009 XLIX, 481 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 453 Methods in enzymology ... Klionsky, Daniel J. Sonstige oth (DE-604)BV035270787 3 Methods in enzymology 453 (DE-604)BV000000938 453 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348280&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Autophagy Methods in enzymology |
| title | Autophagy |
| title_auth | Autophagy |
| title_exact_search | Autophagy |
| title_full | Autophagy C Autophagy in disease and clinical applications ed. by Daniel J. Klionsky |
| title_fullStr | Autophagy C Autophagy in disease and clinical applications ed. by Daniel J. Klionsky |
| title_full_unstemmed | Autophagy C Autophagy in disease and clinical applications ed. by Daniel J. Klionsky |
| title_short | Autophagy |
| title_sort | autophagy autophagy in disease and clinical applications |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017348280&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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