The case for extending storage and secretion functions of human mast cell granules to include synthesis: with 11 tables
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| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Jena
Urban & Fischer
2002
|
| Edition: | [Bindeeinh.] |
| Series: | Progress in histochemistry and cytochemistry
37,3 |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | S. 234 - 318 Ill., graph. Darst. |
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| 245 | 1 | 0 | |a The case for extending storage and secretion functions of human mast cell granules to include synthesis |b with 11 tables |c Ann M. Dvorak ; Ellen S. Morgan |
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| 490 | 1 | |a Progress in histochemistry and cytochemistry |v 37,3 | |
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Record in the Search Index
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|---|---|
| adam_text | Contents
1 Introduction 237
2 Routine ultrastructural preparations of HMCs reveal granule-associated
ribosomes 238
3 Regressive ethylene diamine tetraacetic acid (EDTA) staining of HMCs
reveals granule-associated ribonuclear proteins (RNPs) 243
4 Ribonuclease-gold reveals heparin in HMC granules 247
4.1 RNase-gold-labeled subcellular sites in HMCs 248
4.2 Experiments to determine the basis of RNase-gold staining of HMC
granules 252
4.3 Effect of general inhibitors, blockers and enzyme digestions on RNase-
gold labeling of HMC granules 252
4.4 Effect of specific blocking and enzyme digestions on RNase-gold labe¬
ling of HMC granules 257
4-5 RNase-gold staining of agar blocks containing heparin, RNA, or hista-
mine 258
*-6 Comment regarding an ultrastructural method that demonstrates heparin
based on the known property of heparin as an inhibitor of nbonuclease . 262
^ Ultrastructural autoradiography detects incorporation of tritiated uridi-
ne into HMC granule RNA 263
D-l [3H]-uridine labels HMC secretory granules 266
3 2 [3H]-uridine subcellular localization in control HMCs 266
3-3 [3H]-uridine granule label changes in degranulating and recovering
HMCs 270
3-4 The effect of isotope pulse time on incorporation of [3H]-uridine in
HMCs 273
3-5 Impact of recovery time on [3H]-uridine incorporation in HMCs reco¬
vering from degranulation 275
¦ ¦ Comment regarding ultrastructural auto-radiographic detection of [3H]-
uridine (indicating RNA) in HMCs 276
6 Ultrastructural immunogold stains detect undine in HMC granule
RNA 277
7 Ultrastructural in situ hybridization (ISH) detects mRNA in HMC
granules 279
¦ Ultrastructural ISH using [3H]-poIyuridine 281
¦- A polyuridine-gold affinity reagent detects mRNA in electron micros-
topic samples 283
Ultrastructural ISH with biotinylated polyuridine 286
¦¦* A BSA-conjugated polyuridine-gold affinity probe detects mRNA in
electron microscopic samples 290
234 • Contents
7.5 Ultrastructural ISH with polyuridine, detected with an antibody to
undine and a post-embedding immunogold procedure 292
8 Immunogold studies of HMCs with specific autoantibody-containing
human sera detect RNA-associated protein antigens of different RNA
species in and associated with secretory granules 294
8.1 Immunogold stains of HMC granules for Ro, La, RNP, Sm, and P anti¬
gens 297
8.2 Comment regarding immunogold studies with human autoantibody-
containing sera which demonstrate RNA-associated protein antigens of
different RNA species in HMC granules 302
9 HMC secretory-storage granules contain RNA machinery important for
protein synthesis - conclusions 303
10 RNA on the move (Wilhelm and Vale, 1993): Site-specific synthesis-
comment 305
References 308
i
|
| any_adam_object | 1 |
| author | Dvorak, Ann M. Morgan, Ellen S. |
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| bvnumber | BV035297235 |
| ctrlnum | (OCoLC)248537817 (DE-599)GBV350452350 |
| edition | [Bindeeinh.] |
| format | Book |
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| id | DE-604.BV035297235 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:30:42Z |
| institution | BVB |
| language | English |
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| physical | S. 234 - 318 Ill., graph. Darst. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Urban & Fischer |
| record_format | marc |
| series | Progress in histochemistry and cytochemistry |
| series2 | Progress in histochemistry and cytochemistry |
| spelling | Dvorak, Ann M. Verfasser (DE-588)123969484 aut The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables Ann M. Dvorak ; Ellen S. Morgan [Bindeeinh.] Jena Urban & Fischer 2002 S. 234 - 318 Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Progress in histochemistry and cytochemistry 37,3 Morgan, Ellen S. Verfasser aut Progress in histochemistry and cytochemistry 37,3 (DE-604)BV000005277 37,3 DE-605 pdf/application http://www.gbv.de/dms/hbz/toc/ht013399871.pdf 2008-11-15 Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017102169&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dvorak, Ann M. Morgan, Ellen S. The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables Progress in histochemistry and cytochemistry |
| title | The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables |
| title_auth | The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables |
| title_exact_search | The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables |
| title_full | The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables Ann M. Dvorak ; Ellen S. Morgan |
| title_fullStr | The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables Ann M. Dvorak ; Ellen S. Morgan |
| title_full_unstemmed | The case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables Ann M. Dvorak ; Ellen S. Morgan |
| title_short | The case for extending storage and secretion functions of human mast cell granules to include synthesis |
| title_sort | the case for extending storage and secretion functions of human mast cell granules to include synthesis with 11 tables |
| title_sub | with 11 tables |
| url | http://www.gbv.de/dms/hbz/toc/ht013399871.pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017102169&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000005277 |
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