Protein blotting and detection: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Dordrecht [u.a.]
Humana Press
2009
|
| Series: | Methods in molecular biology
536 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 588 S. Ill., graph. Darst. |
| ISBN: | 9781934115732 9781597455428 |
Staff View
MARC
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| 245 | 1 | 0 | |a Protein blotting and detection |b methods and protocols |c ed. by Biji T. Kurien ... |
| 264 | 1 | |a Dordrecht [u.a.] |b Humana Press |c 2009 | |
| 300 | |a XIII, 588 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 536 | |
| 490 | 0 | |a Springer protocols | |
| 650 | 4 | |a Blotting, Western |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Proteins |x analysis |v Laboratory Manuals | |
| 650 | 4 | |a Western immunoblotting | |
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| 700 | 1 | |a Kurien, Biji T. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 536 |w (DE-604)BV035362695 |9 536 | |
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface
..................................................................................................................
v
Contributors.
......................................................................................................... xi
1.
Origins of Protein Blotting
........................................................................... 1
Harry Towbin
2.
Western Blotting: Remembrance of Past Things
........................................... 5
W. Neal Burnette
3.
Introduction to Protein Blotting
.................................................................. 9
Biji
T.
Kurien
and
R.
Hal Scofield
4.
Simultaneous Immunoblotting Analysis with Activity Gel
Electrophoresis and 2-D Gel Electrophoresis
................................................. 23
Der-Yen Lee and
Geen-Dong
Chang
5.
Diffusion Blotting for Rapid Production of Multiple Identical
Imprints from Sodium Dodecyl
Sulfate Polyacrylamide
Gel Electrophoresis on a Solid Support
......................................................... 35
Ingrid Olsen
and
Harald
G.
Wiker
6.
Tryptic
Peptide
Purification Using Polyvinylidene Difluoride
Membrane for Matrix-Assisted Laser Desorption Ionization
Time of Flight Mass Spectrometry
................................................................ 39
Biji
T.
Kurien
and
R.
Hal Scofield
7.
Affinity Immunoblotting for Analysis of Antibody
Clonotype Distribution in a Lupus Patient
Developing Anti-Ro
60
Over Time
............................................................... 45
Biji
T.
Kurien
and
R.
Hal Scofield
8.
Non-electrophoretic Bi-directional Transfer of a Single SDS-PAGE
Gel with Multiple Antigens to Obtain
12
Immunoblots
................................ 55
Biji
T.
Kurien
and
R.
Hal Scofield
9.
Slice Blotting
................................................................................................ 67
Graeme Lowe
10.
Localizing Proteins by Tissue Printing
............................................................ 75
Rafael F. Pont-Lezica
11.
Dot-Immunobinding Assay
.......................................................................... 89
5.
Sumi,
A. Mathai, and V.V. Radhakrishnan
12.
Capillary Blotting of Glycosaminoglycans on Nitrocellulose
Membranes After
Agarose Gel
Electrophoresis Separation
............................. 95
Nicola
Volpi
and Francesca Maccari
13.
Quantitative Computerized Western Blotting
............................................... 103
Dalit
Talmi-Frank,
Charles L.
Joffe,
and Gad Baneth
vil
viii Contents
14.
Cationic
Electrophoresis and Eastern Blotting..............................................
115
Engelbert Buxbaum
15.
A Miniaturized Blotting System for Simultaneous
Detection of Different Autoantibodies
.......................................................... 129
Ulrich
Canzler,
Holger
Bartsch, Kai Großmann, Werner Lehmann,
Karsten Conrad,
Biji
T.
Kurien, Yaser Dorrt, R. Hai Scofield,
and Michael Bachmann
16. Transfer and
Multiplex Immunoblotting
of a
Paraffin
Embedded Tissue
....... 139
Јооп-Тоцд
Chung and Stephen
M.
Hewitt
17.
Multistrip Western Blotting to Increase Quantitative Data Output
................ 149
Anatoly Kiyatkin and
Edita Aksamitiene
18.
Western Blotting/Edman Sequencing using PVDF Membrane
..................... 163
Setsuko Komatsu
19.
Blotting from PhastGel to Membranes by Ultrasound
.................................. 173
Joseph
Kost
20. Ultrarapid Electrophoretic
Transfer of High
and Low Molecular Weight Proteins Using Heat
.......................................... 181
Biji
T.
Kurien
and
R.
Hal Scofield
21.
Strip Immunoblotting of Multiple Antigenic Peptides
to Nitrocellulose Membrane
......................................................................... 191
Biji
T.
Kurien
22.
Affinity Purification of Autoantibodies from an
Antigen Strip Excised from a Nitrocellulose Protein Blot
.............................. 201
Biji
T.
Kurien
23.
Double-Blotting: A Solution to the Problem of Nonspecific
Binding of Secondary Antibodies in Immunoblotting Procedures
................. 213
Françoise
Lasne
24.
Efficient Electroblotting of Very Large Proteins
Using a Vertical
Agarose
Electrophoresis System
........................................... 221
Marion L. Greaser and Chad M. Warren
25.
Semi-Dry Protein Transfer and Immunodetection of
P-Selectin Using an Antibody to its
C-terminal
Tag
...................................... 229
Padmaja Mehta
26.
The Multiple Antigen Blot Assay: A Simple, Versatile
and Multipurpose Immunoenzymatic Technique
.......................................... 237
Oscar
Noya
Sandra
Losada,
Marilyan
Toledo,
and
Belkisyolé Alarcón de
Noya
27.
Blotting from Immobilized
pH
Gradient Gels:
Application to Total Cell Lysates
.................................................................. 253
Harry Towbin
28.
Immunoprecipitation-Western Blot for Proteins of Low Abundance
............. 259
Edward P. Trieu, Joanne K. Gross, and Ira
N.
Targojf
Contents xi
29.
Native
Electrophoresis and Western
Blot Analysis:
Method and Applications
............................................................................. 277
Ioannis
N.
Manoussopoulos and
Mina
Tsagris
30.
Grid-Immunoblotting
.................................................................................. 289
Olga
Yeliosofand R. Hal Scofield
31.
Sequential Use of Immunoblots for Characterization
of Autoantibody Specificities
........................................................................ 293
Holger
Bartsch
and Michael
Bachmann
32.
Renaturation of
Recombinant
Ro
60 Autoantigen
by Calcium Ions on PVDF Membrane
.......................................................... 299
Biji
T.
Kurien
and Michael
Bachmann
33.
Intermittent Microwave Irradiation Facilitates
Antigen-Antibody Reaction in Western Blot Analysis
.................................... 307
Yu-Ting Liu and Shinya Toyokuni
34.
Detection of Protein-Protein Interactions by Far-Western Blotting
............... 313
Kazuya Machida and Bruce
J
.
Mayer
35.
On-Membrane Tryptic Digestion of Proteins for
Mass Spectrometry Analysis
.......................................................................... 331
Jose L. Luque-Garcia and Thomas A.
Neubert
36.
Western Blot Analysis of Adhesive Interactions under
Fluid Shear Conditions: The Blot Rolling Assay
............................................ 343
Robert
Sackstein
and Robert Fuhlbrigge
37.
ELISPOT Assay on Membrane
Microplates
.................................................. 355
Alexander E. Kalyuzhny
38.
A Brief Review of Other Notable Protein Blotting Methods
......................... 367
Biji
T.
Kurien
and
R.
Hal Scofield
39.
Phosphoprotein Detection on Protein Electroblot
Using a Phosphate-Specific Fluorophore
....................................................... 385
Lee Broderick
Bockus and R. Hal Scofield
40.
Rapid, Antibody-Free Detection of
Recombinant
Proteins on Blots Using Enzyme Fragment Complementation
...................... 395
Neil W. Charter, Joe
Horečka,
Chin-Yee
Loh,
Albert Doan,
Tom Wehrman, and Keith R. Olson
41.
Fluorescent Labeling of Proteins and Its Application
to SDS-PAGE and Western Blotting
............................................................. 407
F. Javier Alba, Salvador
Bartolomé,
Antonio
Bermúdez,
and Joan-Ramon
Daban
42.
Whole and Strip Nitrocellulose Membrane as well as
New Line Immunoblotting of Antigen Using the
Chemiluminescence Technique
..................................................................... 417
Yaser Dorri, AH W. Khalili, and R. Hal Scofield
43.
Protein Stains to Detect Antigen on Membranes
.......................................... 433
Anil D souza and R. Hal Scofield
χ
Contents
44.
Use of Nonradioactive Detection Method
for North- and Southwestern Blot
................................................................ 441
Claudia Franke,
Daniel
Gräfe, Holger Bartsch,
and Michael
Bachmann
45.
Immunoblotting Using
Radiolabeled
Reagents for Detection
....................... 451
Holger Bartsch,
Claudia
Franke,
and Michael
Bachmann
46.
Detection of Protein Carbonyls by Means of Biotin
Hydrazide-Streptavidin Affinity Methods
..................................................... 457
Kenneth Hensley
47.
Rainbow Western Blotting
........................................................................ 463
Stan
(Stanislaw) Krajewski
48.
Multiple Antigen Detection Western Blotting
............................................... 473
Stan Krajewski, Xianshu Huang, and
Maryla Krajewska
49.
Detection of Calcium Binding by Ro
60
Multiple
Antigenic Peptides on PVDF Membrane Using Quin-2
................................ 483
Biji
T.
Kurien
and
Michael Bachmann
50.
Detection of La/SS-B by Western Blot Using
Nanogold-Tagged Antibodies and Silver Enhancement
................................. 491
Shannon Maier, Sherry Hubbell, and R. Hal Scofield
51.
Imaging Systems for Westerns: Chemiluminescence
vs. Infrared Detection
................................................................................... 499
Suresh T. Mathews,
Eric
P.
Plaisance,
and Teayoun Kim
52.
A Fluorescent Codetection System for Immunoblotting
and Proteomics Through ECL-Plex and CyDye Labeling
............................. 515
Ciara
A. McManus, Pamela M. Donoghue, and Michael J. Dunn
53.
Visualization of Unstained Protein Bands on PVDF
...................................... 527
Jun
Park, Masaharu Mabuchi, andAjay Sharma
54.
Improved Protein Detection Using Cold Microwave Technology
................. 533
Aaron G. Smith, Jyothi Jayaram, Carol B. Johnson, E. Ann Ellis,
Stanislav
Vitha, Ellen W. Collisson, and Andreas HoIzenburg
55.
TLC
Blot (Far-Eastern Blot) and Its Applications
......................................... 545
Takao Taki, Tania
Valdes
Gonzalez,
Naoko
Goto-Inoue, Takahiro Hayasaka,
and Mitsutoshi
Setou
56.
A Brief Review of Other Notable Protein
Detection Methods on Blots
......................................................................... 557
Biji
T.
Kurien
and
R.
Hal Scofield
57.
Sending Secret Messages on Nitrocellulose Membrane
and the Use of a Molecular Pen for Orientation
in ECL Membrane Assays
............................................................................. 573
Biji
T.
Kurien
Index
.................................................................................................................... 583
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| id | DE-604.BV035280085 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:30:19Z |
| institution | BVB |
| isbn | 9781934115732 9781597455428 |
| language | English |
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| physical | XIII, 588 S. Ill., graph. Darst. |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Protein blotting and detection methods and protocols ed. by Biji T. Kurien ... Dordrecht [u.a.] Humana Press 2009 XIII, 588 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 536 Springer protocols Blotting, Western methods Laboratory Manuals Proteins analysis Laboratory Manuals Western immunoblotting Blotting-Technik (DE-588)4410947-7 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Blotting-Technik (DE-588)4410947-7 s b DE-604 Kurien, Biji T. Sonstige oth Methods in molecular biology 536 (DE-604)BV035362695 536 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017085307&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein blotting and detection methods and protocols Methods in molecular biology Blotting, Western methods Laboratory Manuals Proteins analysis Laboratory Manuals Western immunoblotting Blotting-Technik (DE-588)4410947-7 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4410947-7 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | Protein blotting and detection methods and protocols |
| title_auth | Protein blotting and detection methods and protocols |
| title_exact_search | Protein blotting and detection methods and protocols |
| title_full | Protein blotting and detection methods and protocols ed. by Biji T. Kurien ... |
| title_fullStr | Protein blotting and detection methods and protocols ed. by Biji T. Kurien ... |
| title_full_unstemmed | Protein blotting and detection methods and protocols ed. by Biji T. Kurien ... |
| title_short | Protein blotting and detection |
| title_sort | protein blotting and detection methods and protocols |
| title_sub | methods and protocols |
| topic | Blotting, Western methods Laboratory Manuals Proteins analysis Laboratory Manuals Western immunoblotting Blotting-Technik (DE-588)4410947-7 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Blotting, Western methods Laboratory Manuals Proteins analysis Laboratory Manuals Western immunoblotting Blotting-Technik Proteine Aufsatzsammlung |
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