Modern biophysical chemistry: detection and analysis of biomolecules
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Weinheim
WILEY-VCH
2009
|
| Subjects: | |
| Online Access: | Inhaltstext Inhaltsverzeichnis |
| Physical Description: | IX, 301 S. Ill., graph. Darst. 240 mm x 170 mm |
| ISBN: | 9783527323609 |
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MARC
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| 100 | 1 | |a Walla, Peter Jomo |d 1970- |e Verfasser |0 (DE-588)120817691 |4 aut | |
| 245 | 1 | 0 | |a Modern biophysical chemistry |b detection and analysis of biomolecules |c Peter Jomo Walla |
| 264 | 1 | |a Weinheim |b WILEY-VCH |c 2009 | |
| 300 | |a IX, 301 S. |b Ill., graph. Darst. |c 240 mm x 170 mm | ||
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| 650 | 4 | |a Biophysikalische Chemie | |
| 650 | 4 | |a Biomolecules |x Analysis | |
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Record in the Search Index
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| adam_text |
Contents
Introduction
1
Part One Basic
Methods in Biophysical Chemistry
13
Ί
Basic Optical Principles
25
1.1
Introduction
ÍS
1.2
What Does the Electronic Structure of Molecules Look like?
Orbitals,
Wave Functions and Bonding Interactions
16
1.3
How Does light Interact with Molecules? Transition Densities
and the Transition
Dipole
Moment
22
1.4
Absorption Spectra of Molecules in Liquid Environments.
Vibrational Excitation and the Franck-Condon Principle
26
1.5
What Happens After Molecules have Absorbed light? Fluorescence,
Non-Radiative Transitions and the Triplet State
29
1.6
Quantitative Description of all Processes: Quantum Efficiencies,
Kinetics of Excited State Populations and the
Jabłoński
Diagram
34
Bibliography
40
2
Optical Properties of Biomolecules
41
2.1
Introduction
41
2.2
Experimental Determination of Absorption and Fluorescence
Spectra
41
2.3
Optical Properties of Proteins and
DNA 45
2.3.1
Intrinsic Absorption and Fluorescence of
Amino
Acids, Peptides
and Proteins
45
2.3.2
Intrinsic Absorption of Nudeotides,
DNA
and
RNA
47
2.4
Optical Properties of Important Cofactors
49
2.4.1
Haem
49
2.4.2
Nicotmamide
Adenine Dinudeotides 52
2.4.3
Flavins
53
2.4.4
Chlorophylls
54
VIII Contents
8.3.1
Autocorrélation
Analysis and Observable Key Parameters
194
8.3.2
Autocorrelation Analysis, Mathematical Background
199
8.3.3
Quantitative Determination of Important Parameters from
Autocorrelation Curves
201
8.3.3.1
Relationship Between Volume and Molecular Mass of a Protein
and its Diffusion Time
201
8.3.3.2
Two-Dimensional Diffusion and Active Transport
202
8.3.3.3
Mixtures of Fluorescing Particles
203
8.3.4
Further Correlation Effects
203
8.3.5
Cross-Correlation Analysis
205
8.4
Optical Tweezers
209
8.4.1
Theoretical Background
210
8.4.2
Application Examples
216
8.4.2.1
Unfolding of
RNA
and
DNA
Hairpins
216
8.4.2.2
RNA Polymerase
217
8.4.2.3 DNA
Polymerase
219
8.5
Atomic Force Microscopy of Biomolecules
220
8.5.1
Principle of an AFM
220
8.5.2
Application Examples
221
8.5.2.1
Unfolding of
DNA
Hairpins
221
8.5.2.2
Receptor-ligand Binding Forces
223
8.5.2.3
Protein Unfolding
223
8.6
Patch Clamping
224
8.6.1
Ion Channels
224
8.6.2
Patch Clamp Configurations
226
Bibliography
232
9
Ultrafast- and Nonlinear Spectroscopy
237
9.1
Introduction
237
9.2
Nonlinear Microscopy and Spectroscopy
238
9.2.1
Multi-Photon Excitation
238
9.2.2
Advantages and Disadvantages of Two-Photon Excitation in
Fluorescence Microscopy
239
9.2.3
How are Nonlinear Optical Signals Observed From Biological
Samples?
242
9.2.4
Further Distinct Properties and Advantages of Two-Photon
Excitation
243
9.2.5
Wavemixing and Other Nonlinear Optical Techniques
245
9.3
Ultrafast Spectroscopy
249
9.3.1
Pump-Probe Spectroscopy
249
9.3.2
Application Example: Ultrafast light-Harvesting and Energy
Conversion in Photosynthesis
253
9.3.2.1
Chlorophyll
b
-►
Chlorophyll a
-»
Reaction Centre Energy Flow
255
9.3.2.2
Carotenoid
-»
Chlorophyll Energy Flow
257
Bibliography
263
Contents
IX
10 Special
Techniques
265
10.1
Introduction
265
10.2 Fluorescing
Nanopartícles
265
10.3
Surface Plasmon
Resonance Detection
270
10.4 DNA
Sequencing
272
10.5 DNA Microarrays 273
10.6
Flow Cytometry
276
10.7
Fluorescence In Situ Hybridization
277
10.8 Microspheres and Nanospheres 278
Bibliography
280
Π
Assay
Development, Readers
and High-Throughput Screening
281
11.1
Introduction
281
11.2
Assay Development and Assay Quality
281
11.3
Microtitre
Plates and Fluorescence Readers
284
11.4
Application Example: Drug Discovery and High-Throughput
Screening
289
Bibliography
294
Index
297 |
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| discipline | Chemie / Pharmazie Biologie Chemie |
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| institution | BVB |
| isbn | 9783527323609 |
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| physical | IX, 301 S. Ill., graph. Darst. 240 mm x 170 mm |
| publishDate | 2009 |
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| spelling | Walla, Peter Jomo 1970- Verfasser (DE-588)120817691 aut Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla Weinheim WILEY-VCH 2009 IX, 301 S. Ill., graph. Darst. 240 mm x 170 mm txt rdacontent n rdamedia nc rdacarrier Biophysikalische Chemie Biomolecules Analysis Physical biochemistry Biophysikalische Chemie (DE-588)4291844-3 gnd rswk-swf Biophysikalische Chemie (DE-588)4291844-3 s DE-604 text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3152692&prov=M&dok_var=1&dok_ext=htm Inhaltstext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017077035&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Walla, Peter Jomo 1970- Modern biophysical chemistry detection and analysis of biomolecules Biophysikalische Chemie Biomolecules Analysis Physical biochemistry Biophysikalische Chemie (DE-588)4291844-3 gnd |
| subject_GND | (DE-588)4291844-3 |
| title | Modern biophysical chemistry detection and analysis of biomolecules |
| title_auth | Modern biophysical chemistry detection and analysis of biomolecules |
| title_exact_search | Modern biophysical chemistry detection and analysis of biomolecules |
| title_full | Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla |
| title_fullStr | Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla |
| title_full_unstemmed | Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla |
| title_short | Modern biophysical chemistry |
| title_sort | modern biophysical chemistry detection and analysis of biomolecules |
| title_sub | detection and analysis of biomolecules |
| topic | Biophysikalische Chemie Biomolecules Analysis Physical biochemistry Biophysikalische Chemie (DE-588)4291844-3 gnd |
| topic_facet | Biophysikalische Chemie Biomolecules Analysis Physical biochemistry |
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