Wnt signaling: 1 Pathway methods and mammalian models
Gespeichert in:
Format: | Buch |
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Sprache: | English |
Veröffentlicht: |
New York, NY
Humana Press , Springer
2008
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Schriftenreihe: | Methods in molecular biology
468 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XV, 334 S. Ill., graph. Darst. |
ISBN: | 9781588299123 |
Internformat
MARC
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245 | 1 | 0 | |a Wnt signaling |n 1 |p Pathway methods and mammalian models |c ed. by Elizabeth Vincan |
264 | 1 | |a New York, NY |b Humana Press , Springer |c 2008 | |
300 | |a XV, 334 S. |b Ill., graph. Darst. | ||
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337 | |b n |2 rdamedia | ||
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490 | 1 | |a Methods in molecular biology |v 468 | |
490 | 0 | |a Methods in molecular biology |v ... | |
650 | 4 | |a Cellular signal transduction | |
650 | 4 | |a Signal Transduction | |
650 | 4 | |a Wnt Proteins | |
650 | 4 | |a Wnt Proteins / analysis / methods | |
650 | 4 | |a Wnt genes | |
650 | 4 | |a Wnt proteins | |
700 | 1 | |a Vincan, Elizabeth |e Sonstige |4 oth | |
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Datensatz im Suchindex
_version_ | 1804138529883684864 |
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adam_text | Titel: Bd. 1. Wnt signaling. Pathway methods and mammalian models
Autor:
Jahr: 2008
Contents
Preface v
Contents vii
Contributors ix
Part I. Wnt Signaling Pathway Methods
Section A. Canonical Wnt/FZD Signaling
1. The Canonical Wnt/(3-Catenin Signalling Pathway. 3
Nick Barker
2. Isolation and Application of Bioactive Wnt Proteins 17
KarlH. Willert
3. Purification and Wnt-Inhibitory Activities of Secreted
Frizzled-Related Proteins 31
Vladimir Wolf, Toshimi Endo, and Jeffrey S. Rubin
4. Measuring GSK3 Expression and Activity in Cells 45
Adam R. Cole and Calum Sutherland
5. Inhibition of Glycogen Synthase Kinase-3 67
Andrei V. Ougolkov and Daniel D. Billadeau
6. Detection of Cytoplasmic and Nuclear Localization
of Adenomatous Polyposis Coli (APC) Protein in Cells 77
Mariana Brocardo and Beric R. Henderson
7. Detection of (3-Catenin Localization by Immunohistochemistry 91
Nick Barker and Maaike van den Born
8: Assaying |3-Catenin/TCF Transcription with P-Catenin/TCF
Transcription-Based Reporter Constructs 99
Travis L. Biechele and Randall T. Moon
9. Native Promoter Reporters Validate Transcriptional Targets Ill
Otto Schmalhofer, Simone Spaderna, and Thomas Brabletz
Section B. Non-canonical Signaling
10. (3-Catenin-Independent Wnt Pathways: Signals,
Core Proteins, and Effectors 131
Richard G. James, William H. Conrad, and Randall T. Moon
11. Image Analysis of Calcium Release Dynamics 145
Christina M. Freisinjjer, Douglas W. Houston, and Diane C. Slusarski
12. Detecting PKC Phosphorylation as Part of the Wnt/Calcium
Pathway in Cutaneous Melanoma 157
Samudra K. Dissanayake and Ashani T. Weeramtna
13. Measuring CamKII Activity in Xenopus Embryos
as a Read-out for Non-canonical Wnt Signaling 173
Michael Kiihl and Petra Pandur
vii
viii Contents
14. Analysis of Wnt7a-Stimulated JNK Activity and cjun
Phosphorylation in Non-Small Cell Lung Cancer Cells 187
Lynn E. Heasley and Robert A. Winn
15. ROCK Enzymatic Assay 197
John D. Domn and Marc D. Jacobs
16. Detection of Planar Polarity Proteins in Mammalian Cochlea 207
Mireille Montcouquiol, Jennifer M. Jones, and Nathalie Sans
Part II. Mammalian Model Systems for Wnt/FZD Function
17. Proteomic Analyses of Protein Complexes in the Wnt Pathway 223
Stepbane Angers
18. In Situ Hybridization to Evaluate the Expression of Wnt
and Frizzled Genes in Mammalian Tissues 231
Kestutis Planutis, Marina Planutiene, and Randall F. Holcombe
19. Assaying Wnt5A-Mediated Invasion in Melanoma Cells 243
Michael P. O Connett, Amanda D. French, Poloko D. Leotlela,
and Ashani T. Weeraratna
20. Coculture Methodologies for the Study of Wnt Signals 255
Kestutis Planutis, Marina Planutiene, and Randall F. Holcombe
21. Analysis of Wnt/FZD-Mediated Signalling in a Cell Line
Model of Colorectal Cancer Morphogenesis 263
Elizabeth Vincan, Robert H. Whitehead, and Maree C. Faux
22. Analysing Tissue and Gene Function in Intestinal Organ Culture 275
Helen E. Abud, Heather M. Young, and Donald F. Newjjreen
23. Genetics of Wnt Signaling During Early Mammalian Development 287
Terry P. Tamaguchi
24. Tissue-Specific Transgenic, Conditional Knockout and Knock-In
Mice of Genes in the Canonical Wnt Signaling Pathway 307
Koji Aoki and Makoto M. Taketo
Index 333
i
|
any_adam_object | 1 |
building | Verbundindex |
bvnumber | BV035250967 |
ctrlnum | (OCoLC)300161164 (DE-599)BVBBV035250967 |
dewey-full | 599.1 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 599 - Mammalia |
dewey-raw | 599.1 |
dewey-search | 599.1 |
dewey-sort | 3599.1 |
dewey-tens | 590 - Animals |
discipline | Biologie |
format | Book |
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id | DE-604.BV035250967 |
illustrated | Illustrated |
indexdate | 2024-07-09T21:29:39Z |
institution | BVB |
isbn | 9781588299123 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017056612 |
oclc_num | 300161164 |
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owner | DE-12 DE-83 DE-29T DE-29 |
owner_facet | DE-12 DE-83 DE-29T DE-29 |
physical | XV, 334 S. Ill., graph. Darst. |
publishDate | 2008 |
publishDateSearch | 2008 |
publishDateSort | 2008 |
publisher | Humana Press , Springer |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology |
spelling | Wnt signaling 1 Pathway methods and mammalian models ed. by Elizabeth Vincan New York, NY Humana Press , Springer 2008 XV, 334 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 468 Methods in molecular biology ... Cellular signal transduction Signal Transduction Wnt Proteins Wnt Proteins / analysis / methods Wnt genes Wnt proteins Vincan, Elizabeth Sonstige oth (DE-604)BV035250963 1 Erscheint auch als Online-Ausgabe 978-1-59745-249-6 Methods in molecular biology 468 (DE-604)BV035362695 468 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017056612&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Wnt signaling Methods in molecular biology Cellular signal transduction Signal Transduction Wnt Proteins Wnt Proteins / analysis / methods Wnt genes Wnt proteins |
title | Wnt signaling |
title_auth | Wnt signaling |
title_exact_search | Wnt signaling |
title_full | Wnt signaling 1 Pathway methods and mammalian models ed. by Elizabeth Vincan |
title_fullStr | Wnt signaling 1 Pathway methods and mammalian models ed. by Elizabeth Vincan |
title_full_unstemmed | Wnt signaling 1 Pathway methods and mammalian models ed. by Elizabeth Vincan |
title_short | Wnt signaling |
title_sort | wnt signaling pathway methods and mammalian models |
topic | Cellular signal transduction Signal Transduction Wnt Proteins Wnt Proteins / analysis / methods Wnt genes Wnt proteins |
topic_facet | Cellular signal transduction Signal Transduction Wnt Proteins Wnt Proteins / analysis / methods Wnt genes Wnt proteins |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017056612&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035250963 (DE-604)BV035362695 |
work_keys_str_mv | AT vincanelizabeth wntsignaling1 |