Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells:
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| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
2003
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Jena, Univ., Diss., 2003 (Nicht für den Austausch) |
| Physical Description: | 90 Bl. graph. Darst. : 30 cm |
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| 245 | 1 | 0 | |a Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells |c von Yuantao Liu |
| 246 | 1 | 3 | |a Involvement of PI3Kgamma in the regulation of erythroid differentiation of K562 cells |
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Record in the Search Index
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CONTENTS
1. INTRODUCTION 1
1.1 BACKGROUND-1
1.2 PHOSPHOINOSITIDE 3-KINASES-1
1.2.1 CLASS I P13KS
-J
1.2.2 CLASS U PI3KS
-9
1.2.3 CLASS ILL PI3KS
-10
L .3
SIGNALLING PROTEINS DOWNSTREAM OF PI3KS-10
1.4 P13KS AND CELL DIFFERENTIATION-14
1.4.1 INVOLVEMENT OF PI3KS IN HEMATOPOIESIS
-14
1.4.2 INVOLVEMENT OF PI3KS IN DIFFERENTIATION OF NON-HEMATOPOIETIC CELLS
-18
1.5 AIM OF THE WORK
-20
2. MATERTIALS AND METHODS_21
2.1 MATERIALS-21
2.1.1 CELLS
-21
2.1.2 BACTERIAL STRAINS (E. COLI)
-21
2.1.3 PLASMIDS
-21
2.1.4 PRIMERS FOR RT-PCR
-22
2.1.5 ANTIBODIES
-22
2.1.6 MEDIUM AND SUBSTANCES FOR CELL CULTURE
-23
2.1.7 MATERIALS FOR SILVER STAINING
-24
2.1.8 OTHER REAGENTS AND MATERIALS
-24
2.1.9 BUFFERS AND SOLUTIONS
-25
2.2 METHODS----27
2.2.1 PLASMID PREPARATION
-27
2.2.2 ISOLATION OF CD34* PROGENITORS FROM PERIPHERAL BLOOD
-28
2.2.3 CULTURE OF CD34* CELLS AND INDUCTION OF DIFFERENTIATION
-28
2.2.4 FLOW CYTOMETRY ANALYSIS
-28
2.2.5 K562 CELL CULTURE AND INDUCTION OF DIFFERENTIATION
-29
2.2.6 ASSESSMENT OF ERYTHROID DIFFERENTIATION OF K562 CELLS
-29
2.2.7 STABLE TRANSFECTION OF K562 CELLS
-30
2.2.8 WESTERN BLOT
-30
2.2.9 PURIFICATION OF POLYCLONAL ANTI-PI3KYANTIBODY
-31
2.2.10 COUPLING ANTI-PI3KYMONOCLONAL ANTIBODY TO PROTEIN G- SEPHAROSE
-32
2.2.11 IMMU N OPRECIPITA TION
-33
2.2.12 PI3KYPROTEIN KINASE ACTIVITY ASSAY
-33
2.2.13 SILVER STAINING
-33
2.2.14 RNA ISOLATION AND RT-PCR
-34
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/969062311
3. RESULTS
36
3. L
INVOLVEMENT OF PL3KY IN THE ERYTHROID DIFFERENTIATION OF HEMATOPOIETIC
STEM CELLS-36
3.2 INVOLVEMENT OF PI3KY IN THE ERYTHROID DIFFERENTIATION OF K562
CELLS-38
3.3
INFLUENCE OF SUPPRESSING ENDOGENOUS PI3KY EXPRESSION ON THE ERYTHROID
DIFFERENTIATION OF
K562 CELLS-42
3.4 INFLUENCE OF OVEREXPRESSION OF PI3KY ON THE ERYTHROID
DIFFERENTIATION OF K562 CELLS- 45
3.5 RELATIONSHIP OF
PL3KY KINASE ACTIVITIES TO THE ERYTHROID DIFFERENTIATION OF K562
CELLS-46
3.6 EXPRESSION OF P1 01 IN K562 CELLS-49
3.7 INFLUENCE OF PI3KY ON MAPK ACTIVITY IN K562 CELLS-50
3.8
DOWNSTREAM TARGETS FOR PI3KY PROTEIN KINASE ACTIVITY-54
4 DISCUSSION_58
4.1 PI3KY
IS A NEGATIVE REGULATOR OF THE ERYTHROID DIFFERENTIATION OF K562
CELLS-58
4.2
PI3KY NEGATIVELY REGULATES THE ERYTHROLTI DIFFERENTIATION PRESUMABLY VIA
ITS PROTEIN
KINASE ACTIVITY
60
4.3
THE EFFECT OF PI3KY ON THE ERYTHROID DIFFERENTIATION IS INDEPENDENT OF
PLOL-62
4.4 THE EFFECT OF
PI3KY ON THE ERYTHROID DIFFERENTIATION IS NOT MEDIATED BY MAPK-62
4.5 ATTEMPTS TO IDENTIFY THE DOWNSTREAM TARGETS FOR
PI3KY PROTEIN KINASE ACTIVITY-63
4.6 CONCLUSION-65
4.7
OUTLOOK-65
LITERATURE _67
SUMMARY_ 81
ZUSAMMENFASSUNG _ 83
APPENDIX_ 85
ABBREVIATIONS
Y
-85
ACKNOWLEDGEMENTS-87
LEBENSLAUF-88
SELBSTANDIGKEITSERKLARUNG-89
ERKLARUNG ZUR BEWERBUNG-90 |
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| index_date | 2024-07-02T22:52:39Z |
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| language | English |
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| spelling | Liu, Yuantao 1965- Verfasser (DE-588)124932843 aut Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells von Yuantao Liu Involvement of PI3Kgamma in the regulation of erythroid differentiation of K562 cells 2003 90 Bl. graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2003 (Nicht für den Austausch) Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf Regulation (DE-588)4049075-0 gnd rswk-swf Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 gnd rswk-swf Erythrozyt (DE-588)4070945-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Erythrozyt (DE-588)4070945-0 s Zelldifferenzierung (DE-588)4067536-1 s Regulation (DE-588)4049075-0 s Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016973409&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Liu, Yuantao 1965- Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells Zelldifferenzierung (DE-588)4067536-1 gnd Regulation (DE-588)4049075-0 gnd Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 gnd Erythrozyt (DE-588)4070945-0 gnd |
| subject_GND | (DE-588)4067536-1 (DE-588)4049075-0 (DE-588)4451113-9 (DE-588)4070945-0 (DE-588)4113937-9 |
| title | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells |
| title_alt | Involvement of PI3Kgamma in the regulation of erythroid differentiation of K562 cells |
| title_auth | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells |
| title_exact_search | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells |
| title_exact_search_txtP | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells |
| title_full | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells von Yuantao Liu |
| title_fullStr | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells von Yuantao Liu |
| title_full_unstemmed | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells von Yuantao Liu |
| title_short | Involvement of PI3Kg in the regulation of erythroid differentiation of K562 cells |
| title_sort | involvement of pi3kg in the regulation of erythroid differentiation of k562 cells |
| topic | Zelldifferenzierung (DE-588)4067536-1 gnd Regulation (DE-588)4049075-0 gnd Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 gnd Erythrozyt (DE-588)4070945-0 gnd |
| topic_facet | Zelldifferenzierung Regulation Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase Erythrozyt Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016973409&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT liuyuantao involvementofpi3kgintheregulationoferythroiddifferentiationofk562cells AT liuyuantao involvementofpi3kgammaintheregulationoferythroiddifferentiationofk562cells |