Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR:
Saved in:
| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2004
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | III, 99 Bl. Ill., graph. Darst. 30 cm |
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MARC
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| 100 | 1 | |a Afonin, Sergiy E. |e Verfasser |0 (DE-588)139782028 |4 aut | |
| 245 | 1 | 0 | |a Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR |c von Sergiy Eduardovych Afonin |
| 264 | 1 | |c 2004 | |
| 300 | |a III, 99 Bl. |b Ill., graph. Darst. |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Jena, Univ., Diss., 2004 | ||
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| 650 | 0 | 7 | |a Festkörper-NMR-Spektroskopie |0 (DE-588)4713583-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Strukturaufklärung |0 (DE-588)4183788-5 |2 gnd |9 rswk-swf |
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CONTENTS
PAGE
ZUSAMMENFASSUNG
I
SUMMARY
II
ABBREVIATIONS
AND
SYMBOLS
III
PART
1:
INTRODUCTION
AND
BACKGROUND
1
1.1.
BIOLOGICAL
MEMBRANES
1
1.1.1.
LIPID
COMPONENT
OF
BIOLOGICAL
MEMBRANES
3
1.1.2.
PROTEINS
IN
LIPID
MEMBRANES
4
1.2.
BIOLOGICALLY
RELEVANT
PROTEIN-LIPID
INTERACTIONS
5
1.2.1.
MEMBRANE-ACTIVE
PEPTIDES
AND
PROTEINS
5
1.2.1.1.
FUSOGENIC
PEPTIDES
AND
MEMBRANE
FUSION
6
1.2.1.2.
ANTIMICROBIAL
PEPTIDES
AND
THEIR
MEMBRANOTROPIC
ACTION
8
1.3.
METHODOLOGY
OF
MEMBRANE
PROTEIN
STRUCTURAL
STUDIES
9
1.3.1.
MODEL
MEMBRANE
SYSTEMS
9
1.3.2.
PRODUCTION
OF
MEMBRANE
PROTEINS
AND
PEPTIDES
10
1.3.2.1.
BIOSYNTHETIC
APPROACHES
10
1.3.2.2.
CHEMICAL
PEPTIDE
SYNTHESIS
11
1.3.3.
ANALYSIS
OF
MEMBRANE
PROTEIN
STRUCTURE
IN
LIPID
BILAYERS
12
1.4.
NMR
SPECTROSCOPY
14
1.4.1.
INTRODUCTION
TO
THE
NMR
14
1.4.2.
NMR
IN
SOLIDS
VS.
NMR
IN
SOLUTION
14
1.4.3.
SOLID
STATE
NMR
METHODS
IN
STRUCTURE
ANALYSIS
OF
MEMBRANE
PROTEINS
16
1.4.4.
STRUCTURAL
PARAMETERS
FROM
STATIC
NMR
EXPERIMENTS
17
1.4.5.
CHOICE
OF
NMR
ACTIVE
ISOTOPES
21
CITED
LITERATURE
22
PART
2:
EXPEIMENTAL
27
2.1.
MATERIALS
27
2.2.
FLUORINE-LABELLED
PEPTIDES
28
2.2.1.
PEPTIDE
SYNTHESIS
28
2.2.2.
HPLC
PURIFICATION
29
2.2.3.
MASS
SPECTROMETRY
29
2.2.4.
ASSIGNMENT
OF
L
AND
D-4FPHG
EPIMERS
29
2.3.
FUNCTIONAL
CHARACTERIZATION
OF
PEPTIDES
30
2.3.1.
FLUORESCENCE
ASSAY
FOR
FUSOGENIC
ACTIVITY
30
2.3.2.
ANTIMICROBIAL
INHIBITION
ASSAYS
30
2.3.3.
ELECTRON
MICROSCOPY
TO
DETECT
AGGREGATION
31
2.4
NMR
EXPERIMENTS
31
2.4.1.
PREPARATION
OF
MEMBRANE
DISPERSIONS
31
2.4.2.
PREPARATION
OF
ORIENTED
SAMPLES
31
2.4.3.
'
H
HIGH-RESOLUTIONS
NMR
32
2.4.4.
PEPTIDE-LIPID
INTERACTIONS
STUDIED
BY
19
F-NMR
IN
SOLUTION
32
2.4.5.
SOLID
STATE
NMR
MEASUREMENTS
32
2.5.
DETERMINATION
OF
THE
STRUCTURAL
PARAMETERS
33
2.5.1.
MODELING
OF
SECONDARY
STRUCTURE
ELEMENTS
33
2.5.2.
CALCULATION
OF
PEPTIDE
ALIGNMENT
3
5
CITED
LITERATURE
36
PART
3:
4F-PHENYLGLICINE
AS
A
PEPTIDE
LABEL
FOR
19
F-NMR
38
3.1.
THE
NON-NATURAL
AMINO
ACID
4F-PHG
3
8
3.2.
THE
PEPTIDES
LABELLED
WITH
4F-PHG
39
3.3.
INCORPORATION
OF
THE
4F-PHG
INTO
THE
PEPTIDES
39
3.3.1.
RACEMIZATION
OF
4F-PHG
39
3.3.2.
REMOVAL
OF
TRIFLUOROACETIC
ACID
FROM
THE
PEPTIDES
42
3.3.3.
UV
ABSORPTION
OF
THE
AROMATIC
4F-PHG
SIDE
CHAIN
44
3.4.
STRUCTURAL
AND
FUNCTUAL
STUDIES
OF
THE
4F-PHG
LABELLED
PEPTIDES
44
3.4.1.
4F-PHG
EFFECT
ON
HELICAL
STRUCTURE
44
3.4.2.
FUSION
ACTIVITIES
OF
THE
B
18
PEPTIDE
ANALOGUES
CONTAINING
4F-PHG
46
3.4.3.
ANTIMICROBIAL
ACTIVITIES
OF
THE
A-HELICAL
AND
P-SHEET
PEPTIDES
47
3.4.4.
ELECTROSTATIC
INTERACTIONS
OF
PGLA
WITH
LIPID
BILAYERS
50
CITED
LITERATURE
55
PART
4:
MEMBRANE-BOUND
STRUCTURE
OF
THE
FUSOGENIC
PEPTIDE
B18
58
4.1.
THE
FUSOGENIC
PEPTIDE
B
18
58
4.2.
STRUCTURE
DETERMINATION
STRATEGY
AND
USED
ASSUMPTIONS
60
4.3.
19
F-NMR
SPECTRA
OF
THE
MEMBRANE
BOUND
PEPTIDES
61
4.4.
19
F
CSA
TENSOR
ELEMENTS
IN
4F-PHG
62
4.5.
DETERMINATION
OF
PEPTIDE
CONFORMATION
AND
ALIGNMENT
63
4.6.
MOTIONAL
AVERAGING
OF
THE
STRUCTURE
66
4.7.
THE
BOOMERANG-LIKE
INSERTION
-
IS
IT
UBIQUITOUS
FOR
FUSOGENIC
PEPTIDES?
69
CITED
LITERATURE
70
PART
5:
MEMBRANE-BOUND
STRUCTURE
OF
THE
ANTIMICROBIAL
PEPTIDE
GRAMICIDIN
S
71
5.1.
THE
ANTIMICROBIAL
PEPTIDE
GRAMICIDIN
S
71
5.2.
19
F
19
F
DIPOLAR
COUPLING
AS
PARAMETER
FOR
STRUCTURE
VERIFICATION
72
5.3.
ALIGNMENT
OF
MEMBRANE-BOUND
GRAMICIDIN
S
AT
LOW
CONCENTRATION
76
5.4.
RE-ALIGNMENT
OF
GRAMICIDIN
S
AT
HIGH
CONCENTRATION
77
5.4.1.
INFLUENCE
OF
PHYSICO-CHEMICAL
FACTORS
79
5.4.1.1.
INFLUENCE
OF
TEMPERATURE
79
5.4.1.2.
INFLUENCE
OF
PH
81
5.4.1.
,3.INFLUENCE
OF
HYDRATION
82
5.4.2.
INFLUENCE
OF
MEMBRANE
COMPOSITION
83
5.4.2.1.
INFLUENCE
OF
THE
LIPID
ACYL
CHAIN
LENGTH
84
5.4.2.2.
INFLUENCE
OF
BACTERIAL
LIPIDS
85
5.4.2.3.
INFLUENCE
OF
CHOLESTEROL
86
5.5.
DOES
GRAMICIDIN
S
FORM
AN
OLIGOMERIC
PORE?
88
CITED
LITERATURE
90
PART
6:
OUTLOOK
AND
PERSPECTIVES
93
ACKNOWLEDGEMENTS
IV
APPENDIX
I
V
APPENDIX
II
VI |
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| author | Afonin, Sergiy E. |
| author_GND | (DE-588)139782028 |
| author_facet | Afonin, Sergiy E. |
| author_role | aut |
| author_sort | Afonin, Sergiy E. |
| author_variant | s e a se sea |
| building | Verbundindex |
| bvnumber | BV035166376 |
| ctrlnum | (OCoLC)253555379 (DE-599)BVBBV035166376 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Thesis Book |
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| id | DE-604.BV035166376 |
| illustrated | Illustrated |
| index_date | 2024-07-02T22:52:38Z |
| indexdate | 2024-11-22T17:43:55Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016973402 |
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| physical | III, 99 Bl. Ill., graph. Darst. 30 cm |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| record_format | marc |
| spelling | Afonin, Sergiy E. Verfasser (DE-588)139782028 aut Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR von Sergiy Eduardovych Afonin 2004 III, 99 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2004 Lipide (DE-588)4035873-2 gnd rswk-swf Doppelschicht (DE-588)4150451-3 gnd rswk-swf Festkörper-NMR-Spektroskopie (DE-588)4713583-9 gnd rswk-swf Strukturaufklärung (DE-588)4183788-5 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Lipide (DE-588)4035873-2 s Doppelschicht (DE-588)4150451-3 s Membranproteine (DE-588)4130026-9 s Strukturaufklärung (DE-588)4183788-5 s Festkörper-NMR-Spektroskopie (DE-588)4713583-9 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016973402&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Afonin, Sergiy E. Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR Lipide (DE-588)4035873-2 gnd Doppelschicht (DE-588)4150451-3 gnd Festkörper-NMR-Spektroskopie (DE-588)4713583-9 gnd Strukturaufklärung (DE-588)4183788-5 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4035873-2 (DE-588)4150451-3 (DE-588)4713583-9 (DE-588)4183788-5 (DE-588)4130026-9 (DE-588)4113937-9 |
| title | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR |
| title_auth | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR |
| title_exact_search | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR |
| title_exact_search_txtP | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR |
| title_full | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR von Sergiy Eduardovych Afonin |
| title_fullStr | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR von Sergiy Eduardovych Afonin |
| title_full_unstemmed | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR von Sergiy Eduardovych Afonin |
| title_short | Structural studies on membrane active peptides in lipid bilayers by solid state 19F-NMR |
| title_sort | structural studies on membrane active peptides in lipid bilayers by solid state 19f nmr |
| topic | Lipide (DE-588)4035873-2 gnd Doppelschicht (DE-588)4150451-3 gnd Festkörper-NMR-Spektroskopie (DE-588)4713583-9 gnd Strukturaufklärung (DE-588)4183788-5 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Lipide Doppelschicht Festkörper-NMR-Spektroskopie Strukturaufklärung Membranproteine Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016973402&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT afoninsergiye structuralstudiesonmembraneactivepeptidesinlipidbilayersbysolidstate19fnmr |