Basic methods in protein purification and analysis: a laboratory manual
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Cold Spring Harbor, N.Y
Cold Spring Harbor Laboratory Press
2009
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VII, 436 S. Ill., graph. Darst. |
| ISBN: | 9780879698683 9780879698676 |
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| 245 | 1 | 0 | |a Basic methods in protein purification and analysis |b a laboratory manual |c ed. by Richard J. Simpson ... |
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Datensatz im Suchindex
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| adam_text | Titel: Basic methods in protein purification and analysis
Autor: Simpson, Richard J.
Jahr: 2009
Contents
A Note from the Publisher, vii
1. Introduction to Protein Purification Strategies, 1
R.J. Simpson, T. Pawson, G. Gish, P. Adams, and E.A. Golemis
2 Preparation of Cellular and Subcellular Extracts, 39
RJ. Simpson
SECTION 1: PURIFICATION USING GEL ELECTROPHORESIS
3. One-dimensional Polyacrylamide Gel Electrophoresis, 79
R.J. Simpson
4. Preparative Two-dimensional Gel Electrophoresis with Immobilized
pH Gradients, 113
W.R. Stochaj, T. Berkelman, and N. Laird
SECTION 2: PURIFICATION USING COLUMN CHROMATOGRAPHY
5. Ion-exchange Chromatography, 135
B. Westerlund
6. Size-exclusion Chromatography, 153
H. Hedlund
7. Reversed-phase High-performance Liquid Chromatography, 171
C.D. Can and R.L. Moritz
8. Affinity and Immunoaffinity Chromatography, 191
A.J. Courey, S.-H. Lin, and R.L. Moritz
9. Metal Chelate Affinity Chromatography, 209
I. Haneskog
10. Multidimensional Chromatography of Intact Proteins, 231
A. Apffel
SECTION 3: CHARACTERIZING PROTEIN COMPLEXES
11. Identification of Protein-Protein Interactions by Conventional Column
Chromatography, 245
V: Sudakin
vi / Contents
12. Identification of Associated Proteins by Coimmunoprecipitation, 265
P.D. Adams, R. Zhang, M.V. Poustovoitov, S.H. Seeholzer,
andM. Ohh
13. Chromatin Immunoprecipitation of Protein Complexes, 279
A. Breiling and V. Orlando
14. Identification of Protein-Protein Interactions with Glutathione-S-Transferase
Fusion Proteins, 293
M.B. Einarson, E.N. Pugacheva, and J.R. Orlinick
15. Direct Visualization of Protein Interactions in Living Cells Using Bimolecular
Fluorescence Complementation Analysis, 315
C.-D. Hu and T.K. Kerppola
16. In Silico Tools: Analysis for Creating Focused Interaction Networks, 327
R.E. Nasto, I.G. Serebriiskii, M.R Ochs, andE.A. Golemis
APPENDICES
1. Measuring the Concentration of Proteins, 347
R.J. Simpson
2. Concentrating Solutions of Proteins, 369
R.J. Simpson
3. Stabilization of Proteins for Storage, 385
R.J. Simpson
4. Immunoblotting and Staining of Membrane-bound Proteins, 401
R.J. Simpson
5. General Cautions, 417
Index, 425
|
| adam_txt |
Titel: Basic methods in protein purification and analysis
Autor: Simpson, Richard J.
Jahr: 2009
Contents
A Note from the Publisher, vii
1. Introduction to Protein Purification Strategies, 1
R.J. Simpson, T. Pawson, G. Gish, P. Adams, and E.A. Golemis
2 Preparation of Cellular and Subcellular Extracts, 39
RJ. Simpson
SECTION 1: PURIFICATION USING GEL ELECTROPHORESIS
3. One-dimensional Polyacrylamide Gel Electrophoresis, 79
R.J. Simpson
4. Preparative Two-dimensional Gel Electrophoresis with Immobilized
pH Gradients, 113
W.R. Stochaj, T. Berkelman, and N. Laird
SECTION 2: PURIFICATION USING COLUMN CHROMATOGRAPHY
5. Ion-exchange Chromatography, 135
B. Westerlund
6. Size-exclusion Chromatography, 153
H. Hedlund
7. Reversed-phase High-performance Liquid Chromatography, 171
C.D. Can and R.L. Moritz
8. Affinity and Immunoaffinity Chromatography, 191
A.J. Courey, S.-H. Lin, and R.L. Moritz
9. Metal Chelate Affinity Chromatography, 209
I. Haneskog
10. Multidimensional Chromatography of Intact Proteins, 231
A. Apffel
SECTION 3: CHARACTERIZING PROTEIN COMPLEXES
11. Identification of Protein-Protein Interactions by Conventional Column
Chromatography, 245
V: Sudakin
vi / Contents
12. Identification of Associated Proteins by Coimmunoprecipitation, 265
P.D. Adams, R. Zhang, M.V. Poustovoitov, S.H. Seeholzer,
andM. Ohh
13. Chromatin Immunoprecipitation of Protein Complexes, 279
A. Breiling and V. Orlando
14. Identification of Protein-Protein Interactions with Glutathione-S-Transferase
Fusion Proteins, 293
M.B. Einarson, E.N. Pugacheva, and J.R. Orlinick
15. Direct Visualization of Protein Interactions in Living Cells Using Bimolecular
Fluorescence Complementation Analysis, 315
C.-D. Hu and T.K. Kerppola
16. In Silico Tools: Analysis for Creating Focused Interaction Networks, 327
R.E. Nasto, I.G. Serebriiskii, M.R Ochs, andE.A. Golemis
APPENDICES
1. Measuring the Concentration of Proteins, 347
R.J. Simpson
2. Concentrating Solutions of Proteins, 369
R.J. Simpson
3. Stabilization of Proteins for Storage, 385
R.J. Simpson
4. Immunoblotting and Staining of Membrane-bound Proteins, 401
R.J. Simpson
5. General Cautions, 417
Index, 425 |
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| isbn | 9780879698683 9780879698676 |
| language | English |
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| spelling | Basic methods in protein purification and analysis a laboratory manual ed. by Richard J. Simpson ... Cold Spring Harbor, N.Y Cold Spring Harbor Laboratory Press 2009 VII, 436 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Proteins / Purification / Laboratory manuals Proteins / Analysis / Laboratory manuals Chromatography methods Laboratory Manuals Electrophoresis methods Laboratory Manuals Immunoprecipitation methods Laboratory Manuals Proteins Analysis Laboratory manuals Proteins Purification Laboratory manuals Proteins analysis Laboratory Manuals Proteins isolation & purification Laboratory Manuals Reinigung (DE-588)4049250-3 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Chemische Analyse (DE-588)4009840-0 gnd rswk-swf Proteine (DE-588)4076388-2 s Reinigung (DE-588)4049250-3 s Chemische Analyse (DE-588)4009840-0 s Methode (DE-588)4038971-6 s DE-604 Simpson, Richard J. Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016969230&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Basic methods in protein purification and analysis a laboratory manual Proteins / Purification / Laboratory manuals Proteins / Analysis / Laboratory manuals Chromatography methods Laboratory Manuals Electrophoresis methods Laboratory Manuals Immunoprecipitation methods Laboratory Manuals Proteins Analysis Laboratory manuals Proteins Purification Laboratory manuals Proteins analysis Laboratory Manuals Proteins isolation & purification Laboratory Manuals Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd Methode (DE-588)4038971-6 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| subject_GND | (DE-588)4049250-3 (DE-588)4076388-2 (DE-588)4038971-6 (DE-588)4009840-0 |
| title | Basic methods in protein purification and analysis a laboratory manual |
| title_auth | Basic methods in protein purification and analysis a laboratory manual |
| title_exact_search | Basic methods in protein purification and analysis a laboratory manual |
| title_exact_search_txtP | Basic methods in protein purification and analysis a laboratory manual |
| title_full | Basic methods in protein purification and analysis a laboratory manual ed. by Richard J. Simpson ... |
| title_fullStr | Basic methods in protein purification and analysis a laboratory manual ed. by Richard J. Simpson ... |
| title_full_unstemmed | Basic methods in protein purification and analysis a laboratory manual ed. by Richard J. Simpson ... |
| title_short | Basic methods in protein purification and analysis |
| title_sort | basic methods in protein purification and analysis a laboratory manual |
| title_sub | a laboratory manual |
| topic | Proteins / Purification / Laboratory manuals Proteins / Analysis / Laboratory manuals Chromatography methods Laboratory Manuals Electrophoresis methods Laboratory Manuals Immunoprecipitation methods Laboratory Manuals Proteins Analysis Laboratory manuals Proteins Purification Laboratory manuals Proteins analysis Laboratory Manuals Proteins isolation & purification Laboratory Manuals Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd Methode (DE-588)4038971-6 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| topic_facet | Proteins / Purification / Laboratory manuals Proteins / Analysis / Laboratory manuals Chromatography methods Laboratory Manuals Electrophoresis methods Laboratory Manuals Immunoprecipitation methods Laboratory Manuals Proteins Analysis Laboratory manuals Proteins Purification Laboratory manuals Proteins analysis Laboratory Manuals Proteins isolation & purification Laboratory Manuals Reinigung Proteine Methode Chemische Analyse |
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