High throughput protein expression and purification: methods and protocols
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In 'High Throughput Protein Expression and Purification', leading scientists detail the most successful protocols currently in use, includin...
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, N.J.
Humana
2009
|
| Series: | Methods in molecular biology
498 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Summary: | Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In 'High Throughput Protein Expression and Purification', leading scientists detail the most successful protocols currently in use, including high throughput cloning schemes. |
| Physical Description: | XII, 322 S. Ill., graph. Darst. 26cm |
| ISBN: | 9781588298799 |
Staff View
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| adam_text | Contents
Preface
.............................................................
ρ
Contributors
......................................................... ix
1
High-Throughput Protein Production (HTPP): A Review
of Enabling Technologies to Expedite Protein Production
............... 1
Jim Koehn and Ian Hunt
2
Designing Experiments for High-Throughput Protein Expression
......... 19
Stephen P. Chambers and
Susanne
E. Swalley
3
Gateway Cloning for Protein Expression
............................ 31
Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath
4
Flexi Vector Cloning
........................................... 55
Paul G. Blommel, Peter A. Martin,
Kory
D.
Seder, Russell L.
Wróbel,
and Brian G. Fox
5
The Precise Engineering of Expression Vectors Using
High-Throughput In-Fusion™ PCR Cloning
......................... 75
Nick S. Berrow, David Alder ton, and Raymond J. Owens
6
The Polymerase Incomplete Primer Extension (PIPE) Method
Applied to High-Throughput Cloning and Site-Directed Mutagenesis
...... 91
Heath
E. Kloek
and Scott A. Lesley
7
A Family of
LIC
Vectors for High-Throughput Cloning
and Purification of Proteins
...................................... 105
William H. Eschenfeldt, Lucy
Stols,
Cynthia Sanville
Miliard,
Andrzej Joachimiak,
and Mark I. Donnelly
8
System
48
High-Throughput Cloning and Protein
Expression Analysis
............................................ 117
James M. Abdullah,
Andrzej
Joachimiak, and Frank R.
Collari
9
Automated 96-Well Purification of Hexahistidine-Tagged
Recombinant
Proteins on MagneHis Ni2+-Particles
.................... 129
Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery
10
E. coli
and Insect Cell Expression, Automated Purification
and Quantitative Analysis
........................................ 143
Stephen P. Chambers, John R. Fulghum, Douglas A. Austen, Fan
Lu,
and
Susanne
E. Swalley
11
Hexahistidine-Tagged Maltose-Binding Protein as a Fusion
Partner for the Production of Soluble
Recombinant
Proteins in Escherichia
coli
....................................... 157
Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh
12
PHB-Intein-Mediated Protein Purification Strategy
.................... 173
Alison R. Gillies,
Mahmoud
Reza Banki,
and David
W.
Wood
VII
viii Contents
13
High-Throughput Biotinylation of Proteins
.......................... 185
Brian K. Kay, Sang Thai, and Veronica V.
Volpina
14
High-Throughput Insect Cell Protein Expression Applications
........... 199
Mirjam Buchs,
Ernie Kim,
Tann
Pouliquen, Michael Sachs,
Sabine Geisse,
Marion Mahnke, and Ian Hunt
15
High-Throughput Protein Expression Using Cell-Free System
............ 229
Kalavathy Sitaraman and Deb K. Chatter
jee
16
The Production of Glycoproteins by Transient Expression
in Mammalian Cells
............................................ 245
Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens
17
High-Throughput Expression and Detergent Screening
of Integral Membrane Proteins
................................... 265
Said Eshaghi
18
Cell-Free Expression for Nanolipoprotein Particles: Building
a High-Throughput Membrane Protein Solubility Platform
.............. 273
Jenny
A. Cappuccio,
Angela K.
Hinz,
Edward A.
Kuhn,
Julia Fletcher,
Erin S. Arroyo, Paul T. Henderson, Craig D. Blanchette, Vicki L. Walsworth,
Michele
H.
Corzett, Richard J. Law, Joseph B. Pesavento, Brent W.
Segelke,
Todd A. Sulchek, Brett
A. Chromy,
Federico
Katzen, Todd
Peterson,
Graham Bench,
Wiesław
Kudlicki, Paul
D
.
Hoeprichjr,
and Matthew A. Coleman
19
Expression and Purification of Soluble His6-Tagged TEV Protease
......... 297
Joseph
E. Tropea,
Scott Cherry, and David S. Waugh
20
High-Throughput Protein Concentration and Buffer Exchange:
Comparison of
Ultrafiltration
and Ammonium
Sulfate
Precipitation
........ 309
Priscilla A. Moore and Vladimir Kery
Index
........................................................... 315
|
| adam_txt |
Contents
Preface
.
ρ
Contributors
. ix
1
High-Throughput Protein Production (HTPP): A Review
of Enabling Technologies to Expedite Protein Production
. 1
Jim Koehn and Ian Hunt
2
Designing Experiments for High-Throughput Protein Expression
. 19
Stephen P. Chambers and
Susanne
E. Swalley
3
Gateway Cloning for Protein Expression
. 31
Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath
4
Flexi Vector Cloning
. 55
Paul G. Blommel, Peter A. Martin,
Kory
D.
Seder, Russell L.
Wróbel,
and Brian G. Fox
5
The Precise Engineering of Expression Vectors Using
High-Throughput In-Fusion™ PCR Cloning
. 75
Nick S. Berrow, David Alder ton, and Raymond J. Owens
6
The Polymerase Incomplete Primer Extension (PIPE) Method
Applied to High-Throughput Cloning and Site-Directed Mutagenesis
. 91
Heath
E. Kloek
and Scott A. Lesley
7
A Family of
LIC
Vectors for High-Throughput Cloning
and Purification of Proteins
. 105
William H. Eschenfeldt, Lucy
Stols,
Cynthia Sanville
Miliard,
Andrzej Joachimiak,
and Mark I. Donnelly
8
"System
48"
High-Throughput Cloning and Protein
Expression Analysis
. 117
James M. Abdullah,
Andrzej
Joachimiak, and Frank R.
Collari
9
Automated 96-Well Purification of Hexahistidine-Tagged
Recombinant
Proteins on MagneHis Ni2+-Particles
. 129
Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery
10
E. coli
and Insect Cell Expression, Automated Purification
and Quantitative Analysis
. 143
Stephen P. Chambers, John R. Fulghum, Douglas A. Austen, Fan
Lu,
and
Susanne
E. Swalley
11
Hexahistidine-Tagged Maltose-Binding Protein as a Fusion
Partner for the Production of Soluble
Recombinant
Proteins in Escherichia
coli
. 157
Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh
12
PHB-Intein-Mediated Protein Purification Strategy
. 173
Alison R. Gillies,
Mahmoud
Reza Banki,
and David
W.
Wood
VII
viii Contents
13
High-Throughput Biotinylation of Proteins
. 185
Brian K. Kay, Sang Thai, and Veronica V.
Volpina
14
High-Throughput Insect Cell Protein Expression Applications
. 199
Mirjam Buchs,
Ernie Kim,
Tann
Pouliquen, Michael Sachs,
Sabine Geisse,
Marion Mahnke, and Ian Hunt
15
High-Throughput Protein Expression Using Cell-Free System
. 229
Kalavathy Sitaraman and Deb K. Chatter
jee
16
The Production of Glycoproteins by Transient Expression
in Mammalian Cells
. 245
Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens
17
High-Throughput Expression and Detergent Screening
of Integral Membrane Proteins
. 265
Said Eshaghi
18
Cell-Free Expression for Nanolipoprotein Particles: Building
a High-Throughput Membrane Protein Solubility Platform
. 273
Jenny
A. Cappuccio,
Angela K.
Hinz,
Edward A.
Kuhn,
Julia Fletcher,
Erin S. Arroyo, Paul T. Henderson, Craig D. Blanchette, Vicki L. Walsworth,
Michele
H.
Corzett, Richard J. Law, Joseph B. Pesavento, Brent W.
Segelke,
Todd A. Sulchek, Brett
A. Chromy,
Federico
Katzen, Todd
Peterson,
Graham Bench,
Wiesław
Kudlicki, Paul
D
.
Hoeprichjr,
and Matthew A. Coleman
19
Expression and Purification of Soluble His6-Tagged TEV Protease
. 297
Joseph
E. Tropea,
Scott Cherry, and David S. Waugh
20
High-Throughput Protein Concentration and Buffer Exchange:
Comparison of
Ultrafiltration
and Ammonium
Sulfate
Precipitation
. 309
Priscilla A. Moore and Vladimir Kery
Index
. 315 |
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| id | DE-604.BV035123320 |
| illustrated | Illustrated |
| index_date | 2024-07-02T22:21:56Z |
| indexdate | 2024-07-09T21:22:51Z |
| institution | BVB |
| isbn | 9781588298799 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016790949 |
| oclc_num | 241042860 |
| open_access_boolean | |
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| physical | XII, 322 S. Ill., graph. Darst. 26cm |
| publishDate | 2009 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | High throughput protein expression and purification methods and protocols ed. by Sharon A. Doyle ... Totowa, N.J. Humana 2009 XII, 322 S. Ill., graph. Darst. 26cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 498 Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In 'High Throughput Protein Expression and Purification', leading scientists detail the most successful protocols currently in use, including high throughput cloning schemes. Protéines - Séparation ram Proteins / Separation / Methodology Proteine (DE-588)4076388-2 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Reinigungsverfahren (DE-588)4391913-3 gnd rswk-swf Trennverfahren (DE-588)4078395-9 gnd rswk-swf Proteine (DE-588)4076388-2 s Genexpression (DE-588)4020136-3 s DE-604 Trennverfahren (DE-588)4078395-9 s Reinigungsverfahren (DE-588)4391913-3 s Doyle, Sharon A. Sonstige oth Methods in molecular biology 498 (DE-604)BV035362695 498 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016790949&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | High throughput protein expression and purification methods and protocols Methods in molecular biology Protéines - Séparation ram Proteins / Separation / Methodology Proteine (DE-588)4076388-2 gnd Genexpression (DE-588)4020136-3 gnd Reinigungsverfahren (DE-588)4391913-3 gnd Trennverfahren (DE-588)4078395-9 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4020136-3 (DE-588)4391913-3 (DE-588)4078395-9 |
| title | High throughput protein expression and purification methods and protocols |
| title_auth | High throughput protein expression and purification methods and protocols |
| title_exact_search | High throughput protein expression and purification methods and protocols |
| title_exact_search_txtP | High throughput protein expression and purification methods and protocols |
| title_full | High throughput protein expression and purification methods and protocols ed. by Sharon A. Doyle ... |
| title_fullStr | High throughput protein expression and purification methods and protocols ed. by Sharon A. Doyle ... |
| title_full_unstemmed | High throughput protein expression and purification methods and protocols ed. by Sharon A. Doyle ... |
| title_short | High throughput protein expression and purification |
| title_sort | high throughput protein expression and purification methods and protocols |
| title_sub | methods and protocols |
| topic | Protéines - Séparation ram Proteins / Separation / Methodology Proteine (DE-588)4076388-2 gnd Genexpression (DE-588)4020136-3 gnd Reinigungsverfahren (DE-588)4391913-3 gnd Trennverfahren (DE-588)4078395-9 gnd |
| topic_facet | Protéines - Séparation Proteins / Separation / Methodology Proteine Genexpression Reinigungsverfahren Trennverfahren |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016790949&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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