The protein protocols handbook:
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| Format: | Book |
| Language: | English |
| Published: |
New York, NY
Humana Press
2009
|
| Edition: | 3. ed. |
| Series: | Springer protocols handbooks
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXXV, 1985 S. Ill., graph. Darst. |
| ISBN: | 160327474x 9781603274746 |
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| 245 | 1 | 0 | |a The protein protocols handbook |c ed. by John M. Walker |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a New York, NY |b Humana Press |c 2009 | |
| 300 | |a XXXV, 1985 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Springer protocols handbooks | |
| 650 | 4 | |a Chemistry Techniques, Analytical |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Peptides |x analysis |v Laboratory Manuals | |
| 650 | 4 | |a Protein Conformation |v Laboratory Manuals | |
| 650 | 4 | |a Proteins |x Analysis |v Laboratory manuals | |
| 650 | 4 | |a Proteins |x analysis |v Laboratory Manuals | |
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| _version_ | 1804138017913307136 |
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| adam_text | Titel: The protein protocols handbook
Autor: Walker, John M
Jahr: 2009
Contents
Preface..............................................................................................................v
Contributors...................................................................................................xxiii
Part I Quantitation of Proteins
1 Protein Determination by UV Absorption................................................3
Alastair Aitken and Michèle P. Learmonth
2 The Lowry Method for Protein Quantitation............................................7
Jakob H. Waterborg
3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation..............11
John M. Walker
4 The Bradford Method For Protein Quantitation.....................................17
Nicholas J. Kruger
5 Ultrafast Protein Determinations Using Microwave
Enhancement........................................................................................25
Robert E. Akins and Rocky S. Tuan
6 The Nitric Acid Method for Protein Estimation
in Biological Samples............................................................................35
Scott A. Boerner, Crescent R. Isham, Yean Kit Lee,
Jennifer D. Tibodeau, Scott H. Kaufmann, and Keith C. Bible
7 Quantitation of Tryptophan in Proteins..................................................47
Alastair Aitken and Michèle P. Learmonth
8 Kinetic Silver Staining of Proteins.........................................................51
Douglas D. Root and Kuan Wang
9 Quantitation of Cellular Proteins by Flow Cytometry............................55
Thomas D. Friedrich, F. Andrew Ray, Ralph L. Smith,
and John M. Lehman
10 Quantitation of Cellular Proteins by Laser
Scanning Cytometry.............................................................................63
Thomas D. Friedrich, Ralph L. Smith, and John M. Lehman
VII
viii Contents
Part II Electrophoresis of Proteins and Peptides
and Dectection in Gels
11 Protein Solubility in Two-Dimensional Electrophoresis:
Basic Principles and Issues..................................................................73
Thierry Rabilloud
12 Mouse and Human Tissue Sample Preparation
for 2-D Electrophoresis.........................................................................85
Claus Zabel and Joachim Klose
13 Plant Protein Sample Preparation for 2-DE........................................109
Sebastien Christian Carpentier, Rony Swennen,
and Bart Panis
14 Preparation of Bacterial Samples for 2-D PAGE.................................121
Brian B, Vandahl, Gunna Christiansen, and Svend Birkelund
15 Preparation of Bodily Fluids for 2-D PAGE..........................................131
Anthony G. Sullivan, Henry Brzeski, Jeban Ganesalingam,
and Manuel Mayr
16 Immunoaffinity Depletion of High Abundance
Plasma and Serum Proteins...............................................................139
Lynn A. Echan and David W. Speicher
17 Preparation of Yeast Samples for 2-D PAGE......................................155
Joakim Norbeck
18 Membrane Protein Preparation Using Aqueous Polymer
Two-Phase Systems...........................................................................159
Jens Schindler and Hans Gerd Nothwang
19 Subcellular Fractionation of Small Sample Amounts..........................165
Hans Gerd Nothwang, Isabelle Guillemin,
and Jens Schindler
20 Nondenaturing Polyacrylamide Gel
Electrophoresis of Proteins.................................................................171
John M. Walker
21 SDS Polyacrylamide Gel Electrophoresis of Proteins.........................177
John M. Walker
22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins..........187
John M. Walker
23 SDS-Polyacrylamide Gel Electrophoresis of Peptides........................193
Ralph C. Judd
24 Separation of Proteins by Blue Native
Electrophoresis (BN-PAGE)................................................................201
Elke A. Dian, Joachim Rassow, and Christian Motz
Contents ix
25 Separation of Proteins by Gel Electrophoresis
in the Tris-Taurine-HCI System............................................................211
Sylvie Luche, Mireille Chevallet, Cécile Lelong,
and Thierry Rabilloud
26 Cetyltrimethylammonium Bromide Discontinuous
Gel Electrophoresis of Proteins: M-Based Separation
of Proteins with Retained Native Activity............................................221
Robert E. Akins and Rocky S. Tuan
27 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis
of Basic Proteins.................................................................................239
Jakob H. Waterborg
28 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis
of Histones..........................................................................................251
Jakob H. Waterborg
29 Isoelectric Focusing of Proteins in Ultra-Thin
Polyacrylamide Gels....................:......................................................263
John M. Walker
30 Serial Immobilized pH Gradient Isoelectric
Focusing over pH 4-9.........................................................................269
Slobodan Poznanovic, Wojciech Wozny,
Helmut Zengerling, Gerhard P. Schwall,
and Michael A. Cahill
31 Radiolabeling of Eukaryotic Cells and Subsequent
Preparation for 2-Dimensional Electrophoresis...................................279
Nick Bizlos
32 Two-Dimensional Polyacrylamide Gel Electrophoresis
of Proteins Using Carrier Ampholyte pH Gradients
in the First Dimension.........................................................................285
Patricia Gravel
33 Vertical Agarose Electrophoresis and Electroblotting
of High-Molecular-Weight Proteins.....................................................293
Marion L. Greaser and Chad M. Warren
34 Two-Dimensional PAGE of High-Molecular-Mass Proteins.................299
Masamichi Oh-lshi and Tadakazu Maeda
35 Casting Immobilized pH Gradients.....................................................305
Elisabetta Gianazza
36 A Modified Nonequilibrium pH Gradient Electrophoresis
(NEPHGE) Technique for Protein Separation.....................................323
James A. Carroll
x Contents
37 Microchip Capillary Electrophoresis:
Application to Peptide Analysis...........................................................329
Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte
38 Protein Separations in Microfluidic Chips...........................................361
Andrea W. Chow and Bahram Fathollahi
39 Difference Gel Electrophoresis (DIGE)...............................................379
David B. Friedman and Kathryn S. Lilley
40 Comparing 2D Electrophoretic Gels Across
Internet Databases: An Open Source Application..............................409
Peter F. Lemkin, Gregory C. Thornwall, and Jai A. Evans
41 Quantification of Radiolabeled Proteins
in Polyacrylamide Gels.......................................................................443
Wayne R. Springer
42 Differential ProteoTope Radioactive Quantification
of Protein Abundance Ratios..............................................................449
Wojciech Wozny, Gerhard P. Schwall, Chaturvedula S. Sastri,
Slobodan Poznanovic,Werner Stegmann, Christian Hunzinger*,
Karlfried Groebe, and Michael A. Cahill
43 Quantification of Proteins on Polyacrylamide Gels.............................479
Bryan John Smith
44 Using SDS-PAGE and Scanning Laser Densitometry
to Measure Yield and Degradation of Proteins....................................487
Aaron P. Miles and Allan Saul
45 Rapid and Sensitive Staining of Unfixed Proteins
in Polyacrylamide Gels with Nile Red.................................................497
Joan-Ramon Daban, Salvador Bartolomé,
Antonio Bermúdez, and F. Javier Alba
46 Zn2+-Reverse Staining Technique........................................................505
Carlos Fernandez-Patron
47 Protein Staining with Calconcarboxylic
Acid in Polyacrylamide Gels................................................................515
Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin,
and Jung-Kap Choi
48 Detection of Proteins in Polyacrylamide Gels
by Silver Staining................................................................................527
Michael J. Dunn
49 Background-Free Protein Detection in Polyacrylamide
Gels and on Electroblots Using Transition Metal
Chelate Stains.....................................................................................529
Wayne F. Patton
Contents xi
50 Detection of Proteins in Polyacrylamide Gels
by Fluorescent Staining......................................................................547
Michael J. Dunn
51 Detection of Glycoproteins in Gels and Blots......................................555
Nicolle H. Packer, Malcolm S. Ball, Peter L. Devine,
and Wayne F. Patton
52 Staining of Glycoproteins/Proteoglycans on SDS-Gels......................569
Holger J. Moller and Jörgen H. Poulsen
53 Detection of Proteins and Sialoglycoproteins
in Polyacrylamide Gels Using Eosin Y Stain.......................................575
Fan Lin and Gary E. Wise
54 A Modified Pro-Q Diamond Staining Protocol
for Phosphoprotein Detection in Polyacrylamide Gels........................579
Ganesh Kumar Agrawal and Jay J. Thelen
55 Electroelution of Proteins from Polyacrylamide Gels..........................587
Paul Jenö and Martin Horst
56 Autoradiography and Fluorography of Acrylamide Gels......................595
Antonella Circolo and Sunita Gulati
57 Proteolytic Activity Detection by Two-Dimensional
Zymography........................................................................................605
Jeff Wilkesman
Part III Blotting and Detection Methods
58 Protein Blotting by Electroblotting.......................................................617
Mark Page and Robin Thorpe
59 Protein Blotting by the Semidry Method..............................................621
Patricia Gravel
60 Protein Blotting by the Capillary Method.............................................631
John M. Walker
61 Western Blotting of Basic Proteins Electrophoretically
Resolved on Acid-Urea-Triton-Polyacrylamide Gels...........................635
Geneviève P. Delcuve and James R. Davie
62 Immunoblotting of 2-DE Separated Proteins.......................................641
Barbara Magi and Laura Bianchi
63 High-Efficiency Blotting of High-Molecular Weight Proteins................663
Marion L. Greaser and Darl R. Swartz
64 Alkaline Phosphatase Labeling of IgG Antibody.................................673
G. Brian Wisdom
xii Contents
65 ß-Galactosidase Labeling of IgG Antibody..........................................677
G. Brian Wisdom
66 Horseradish Peroxidase Labeling of IgG Antibody.............................681
G. Brian Wisdom
67 Digoxigenin Labeling of IgG Antibody.................................................685
G. Brian Wisdom
68 Conjugation of Fluorochromes to Antibodies......................................687
Su-Yau Mao
69 Coupling of Antibodies with Biotin.......................................................693
Rosaria P. Haugland and Wendy W. You
70 Preparation of Avidin Conjugates........................................................705
Rosaría P. Haugland and Mahesh K. Bhalgat
71 MDPF Staining of Proteins on Western Blots.....................................717
F. Javier Alba and Joan-Ramon Daban
72. Copper Iodide Staining of Proteins and Its
Silver Enhancement............................................................................723
Douglas D. Root and Kuan Wang
73 Detection of Proteins on Blots Using Direct Blue 71...........................729
Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin, and Jung-Kap Choi
74 Detection of Proteins on Western Blots Using
Colorimetrie and Radiometric Visualization
of Secondary Ligands.........................................................................737
Nicholas J. Kruger
75 Identification of Glycoproteins on Nitrocellulose
Membranes Using Lectin Blotting.......................................................755
Patricia Gravel
76 A Sensitive Method to Quantitatively Detect Total Protein
on Membranes after Electrophoretic Transfer Using
Avidin-or Streptavidin-Biotin...............................................................771
William J. LaRochelle
77 Detection and Quantification of Proteins on Immunoblots
using Enhanced Chemiluminescence.................................................779
Jennifer J. Young
78 Reutilization of Western Blots After Chemiluminescent
or Autoradiographic Detection.............................................................789
Scott H. Kaufmann
79 The Use of Quantum Dot Luminescent Probes
for Western Blot Analysis....................................................................807
Savvas C. Makrides, Christina Gasbarro, and Job M. Bello
Contents xiii
80 The Use of Infrared Fluorescent Dyes
in Quantitative Immunoblotting............................................................879
Ching-Hui Yang, Christopher Kasbek, and Harold A. Fisk
81 The Use of Infrared Fluorescent Dyes
in Immunofluorescence Microscopy....................................................827
Christopher Kasbek, Ching-Hui Yang, and Harold A. Fisk
Part IV Chemical Modification of Proteins and Peptide
Production and Purification
82 Carboxymethylation of Cysteine
Using lodoacetamide/lodoacetic Acid.................................................837
Alastair Aitken and Michèle Learmonth
83 Performic Acid Oxidation.....................................................................841
Alastair Aitken and Michèle Learmonth
84 Succinylation of Proteins.....................................................................845
Alastair Aitken and Michèle Learmonth
85 Pyridylethylation of Cysteine Residues...............................................847
Malcolm Ward
86 Side Chain Selective Chemical Modifications of Proteins...................851
Dan S. Tawfik
87 Nitration of Tyrosines...........................................................................855
Dan S. Tawfik
88 Ethoxyformylation of Histidine.............................................................859
Dan S. Tawfik
89 Modification of Arginine Side Chains
with p-Hydroxyphenylglyoxal...............................................................863
Dan S. Tawfik
90 Amidation of Carboxyl Groups............................................................865
Dan S. Tawfik
91 Amidination of Lysine Side Chains.....................................................867
Dan S. Tawfik
92 Modification of Tryptophan with 2-Hydroxy-
5-Nitrobenzylbromide..........................................................................871
Dan S. Tawfik
93 Modification of Sulfhydryl Groups with DTNB.....................................873
Dan S. Tawfik
94 Chemical Cleavage of Proteins at Methionyl-X
Peptide Bonds.....................................................................................875
Bryan John Smith
xiv Contents
95 Chemical Cleavage of Proteins at Tryptophanyl-X
Peptide Bonds.....................................................................................883
Bryan John Smith
96 Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds.............891
Bryan John Smith
97 Chemical Cleavage of Proteins at Cysteinyl-X
Peptide Bonds.....................................................................................895
Bryan John Smith
98 Chemical Cleavage of Proteins at Asparaginyl-Glycyl
Peptide Bonds.....................................................................................899
Bryan John Smith
99 Enzymatic Digestion of Proteins in Solution
and in SDS Polyacrylamide Gels........................................................905
Kathryn L. Stone, Eroi E. Gulcicek, and Kenneth R. Williams
100 On-PVDF Protein Digestions for N-terminal Sequencing
and Peptide Mass Fingerprinting........................................................919
Victoria Pham, William Henzel, and Jennie R. Lili
101 Enzymatic Digestion of Membrane-Bound Proteins
for Peptide Mapping and Internal Sequence Analysis........................927
Joseph Fernandez and Sheenah M. Mische
102 Reverse-Phase HPLC Separation of Enzymatic
Digests of Proteins..............................................................................941
Kathryn L. Stone and Kenneth R. Williams
103 Peptide Mapping by Two-Dimensional Thin-Layer
Electrophoresis-Thin-Layer Chromatography.....................................951
Ralph C. Judd
104 Peptide Mapping by Sodium Dodecyl
Sulfate-Polyacrylamide Gel Electrophoresis.......................................963
Ralph C. Judd
105 Peptide Mapping for Protein Characterization....................................969
Peter Hojrup
106 Production of Protein Hydrolysates Using Enzymes...........................989
John M. Walker and Patricia J. Sweeney
107 Amino Acid Analysis by Precolumn Derivatization
with 1-Fluoro-2,4-Dinitrophenyl-5-i-Alanine Amide
(Marfey s Reagent)..............................................................................995
Sunil Kochhar and Philipp Christen
108 Amino Acid Analysis of Protein Hydrolysates Using Anion
Exchange Chromatography and IPAD Detection..............................1001
Petr Jandik, Jun Cheng, and Nebojsa Avdalovic
Contents xv
109 Validation of Amino Acid Analysis Methods......................................1015
Andrew J. Reason
110 Molecular Weight Estimation for Native Proteins
Using High-Performance Size-Exclusion
Chromatography...............................................................................1029
G. Brent Irvine
111 Detection of Disulfide-Linked Peptides by HPLC..............................1039
Alastair Aitken and Michèle Learmonth
112 Detection of Disulfide-Linked Peptides by Mass
Spectrometry....................................................................................1043
Alastair Aitken and Michèle Learmonth
113 Diagonal Electrophoresis for Detecting Disulfide Bridges.................1047
Alastair Aitken and Michèle Learmonth
114 Estimation of Disulfide Bonds Using Ellman s Reagent....................1053
Alastair Aitken and Michèle Learmonth
115 Quantitation of Cysteine Residues and Disulfide
Bonds by Electrophoresis.................................................................1057
Alastair Aitken and Michèle Learmonth
116 N-Terminal Sequencing of N-Terminally
Modified Proteins..............................................................................1063
Roza Maria Kamp and Hisashi Hirano
117 Deblocking of Proteins Containing N-Terminal
Pyroglutamic Acid.............................................................................1075
Jacek Mozdzanowski
118 Detection and Characterization of Protein
Mutations by Mass Spectrometry......................................................1081
Yoshinao Wada
119 Peptide Sequencing by Nanoelectrospray
Tandem Mass Spectrometry.............................................................1095
Ole Norregaard Jensen and Matthias Wilm
120 Protein Identification by Peptide Mass Fingerprinting
using MALDI-TOF Mass Spectrometry.............................................1117
Judith Webster and David Oxley
121 Protein Ladder Sequencing..............................................................1131
Rong Wang and Brian T. Chait
122 Sequence Analysis with WinGene/WinPep.......................................1141
Lars Hennig
123 HPLC and Mass Spectrometry of Integral
Membrane Proteins...........................................................................1149
Julian P. Whitelegge
xvi Contents
124 Enrichment of Serum Peptides and Analysis
by MALDI-TOF Mass Spectrometry..................................................1167
YanmingAn, Habtom W. Ressom, and Radoslav Goldman
125 Computational Methods for Analysis of MALDI-TOF
Spectra to Discover Peptide Serum Biomarkers...............................1175
Habtom W. Ressom, Rency S. Varghese,
and Radoslav Goldman
Part V Posttranslational Modifications
126 Simple Tools for Complex A/-Glycan Analysis....................................1187
Anne-Catherine Fitchette, Meriem Benchabane,
Thomas Paccalet, Loïc Faye, and Véronique Gomord
127 A Lectin-Binding Assay for the Rapid Characterization
of the Glycosylation of Purified Glycoproteins..................................1205
Mohammad T. Goodarzi, Angeliki Fotinopoulou,
and Graham A. Turner
128 Chemical Methods of Analysis of Glycoproteins...............................1215
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
129 Monosaccharide Analysis by HPAEC...............................................1219
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
130 Monosaccharide Analysis by Gas Chromatography (GC)................1223
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
131 Determination of Monosaccharide Linkage and Substitution
Patterns by GC-MS Methylation Analysis.........................................1227
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
132 Sialic Acid Analysis by HPAEC-PAD.................................................1231
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
133 Chemical Release of O-Linked Oligosaccharide Chains..................1233
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
134 O-Linked Oligosaccharide Profiling by HPLC...................................1235
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
135 O-Linked Oligosaccharide Profiling by HPAEC-PAD.........................1237
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
Contents xvii
136 Release of A/-Linked Oligosaccharide Chains
by Hydrazinolysis..............................................................................1239
Tsuguo Mizuochi and Elizabeth F. Hounsell
137 Enzymatic Release of O-and A/-Linked
Oligosaccharide Chains....................................................................1243
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
138 A/-Linked Oligosaccharide Profiling by H PLC
on Porous Graphitized Carbon (PGC)..............................................1247
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
139 A/-Linked Oligosaccharide Profiling by HPAEC-PAD.........................1249
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
140 HPAEC-PAD Analysis of Monosaccharides Released
by Exoglycosidase Digestion Using the CarboPac
MA1 Column.....................................................................................1253
Michael Weitzhandler, Jeffrey Rohrer, James R. Thayer,
and Nebojsa Avdalovic
141 Microassay Analyses of Protein Glycosylation.................................1261
Nicky K. C. Wong, Nnennaya Kanu, Natasha Thandrayen,
Geert Jan Rademaker, Christopher I. Baldwin,
David V. Renouf, and Elizabeth F. Hounsell
142 Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled
Carbohydrates from Glycoproteins...................................................1273
Brian K. Brandley, John C. Klock, and Christopher M. Starr
143 HPLC Analysis of Fluorescently Labeled Glycans............................1289
Tony Merry and Sviatlana Astrautsova
144 Glycoprofiling Purified Glycoproteins
Using Surface Plasmon Resonance.................................................1313
Angeliki Fotinopoulou and Graham A. Turner
145 Sequencing Heparan Sulfate Saccharides.......................................1321
Jeremy E. Turnbull
146 Analysis of Glycoprotein Heterogeneity by Capillary
Electrophoresis and Mass Spectrometry..........................................1335
Andrew D. Hooker and David C. James
147 Affinity Chromatography of Oligosaccharides
and Glycopeptides with Immobilized Lectins....................................1347
Kazuo Yamamoto
148 In-Gel Enzymatic Release of W-Glycans...........................................1363
David J. Harvey
xviii Contents
149 Analysis of /V-ünked Glycans by Mass Spectrometry......................1371
David J. Harvey
150 MS Analysis of Protein Glycosylation...............................................1387
NobuakiTakemori, Naoka Komori,
and Hiroyuki Matsumoto
151 Mapping protein A/-Glycosylation by COFRADIC.............................1395
Bart Ghesquière, Joël Vandekerckhove, and Kris Gevaert
152 Mass Spectrometric Analysis of O-Linked
Glycans Released Directly from Glycoproteins
in Gels Using ß-Elimination...............................................................1403
Adrian M. Taylor and Jane Thomas-Oates
153 Glycopeptide Analysis Using LC/MS and LC/MSn:
Site-Specific Glycosylation Analysis of a Glycoprotein.....................7479
Satsuki Itoh, Daisuke Takakura, Nana Kawasaki,
and Teruhide Yamaguchi
154 Identification of Vitamin K-Dependent Proteins
Using a Gla-Specific Monoclonal Antibody.......................................1431
Karin Hansson
155 The Identification of Protein S-Nitrosocysteine.................................1451
Todd M. Greco, Sheryl L. Stamer, Daniel C. Liebler,
and Harry Ischiropoulos
156 Detection of Nitrotyrosine-Containing Proteins.................................1467
Xianquan Zhan and Dominic M. Desiderio
157 Mass Spectrometric Determination of Protein
Ubiquitination....................................................................................1491
Carol E. Parker, Maria Warren Hines, Viorel Mocanu,
Susanna F. Greer, and Christoph H. Borchers
158 Detection of Sumoylated Proteins.....................................................1519
Ok-Kyong Park-Sarge and Kevin D. Sarge
159 Efficient Enrichment of Intact Phosphorylated Proteins
by Modified Immobilized Metal-Affinity
Chromatography...............................................................................1531
Anna Dubrovska
160 Analyzing Protein Phosphorylation...................................................1547
John Colyer
161 Mass Spectrometric Analysis of Protein Phosphorylation................1555
Stefan Gander, Alessio Cremonesi, Johana Chicher,
Suzette Moes, and Paul Jenö
Contents xix
162 Protein Microarrays for Phosphorylation Studies..............................1567
Birgit Kersten and Tanja Feilner
163 Two-Dimensional Phosphopeptide Mapping.....................................1579
Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky,
and Steven F. Dowdy
164 Identification of Proteins Modified by Protein
(D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase......................1589
Darin J. Weber and Philip N. McFadden
165 Analysis of Tyrosine-O-Sulfation.......................................................1601
Jens R. Bundgaard, Jette W. Sen, Anders H. Johnsen,
and Jens F. Reh feld
166 Analysis of Protein Palmitoylation
by Metabolic Radiolabeling Methods................................................1623
Katherine H. Pedone, Leah S. Bernstein, Maurine E. Under,
and John R. Hepler
167 Incorporation of Radiolabeled Prenyl Alcohols
and Their Analogs into Mammalian Cell Proteins:
A Useful Tool for Studying Proteins Prenylation................................1637
Alberto Corsini, Christopher C. Farnsworth,
Paul McGeady, Michael H. Gelb, and John A. Glomset
168 The Metabolic Labeling and Analysis
of Isoprenylated Proteins..................................................................1657
Douglas A. Andres, Dean C. Crick, H. Peter Spielmann,
and Charles J. Waechter
Part VI Antibody Techniques
169 Antibody Production..........................................................................1679
Robert Burns
170 Production of Antibodies Using Proteins
in Gel Bands.....................................................................................1687
Sally Ann Amero, Tharappel C. James,
and Sarah C. R. Elgin
171 Raising Highly Specific Polyclonal Antibodies Using
Biocompatible Support-Bound Antigens...........................................1693
Monique Diano and André Le Bivio
172 Production of Antisera Using Peptide Conjugates............................7705
Thomas E. Adrian
173 Small-Molecule-Protein Conjugation Procedures............................1715
Stephen Thompson
xx Contents
174 The Chloramine T Method for Radiolabeling Protein........................1727
Graham S. Bailey
175 The Lactoperoxidase Method
for Radiolabeling Protein...................................................................1731
Graham S. Bailey
176 The Bolton and Hunter Method
for Radiolabeling Protein...................................................................1733
Graham S. Bailey
177 Preparation of 125l-Labeled Peptides and Proteins
with High Specific Activity Using IODO-GEN....................................1735
J. Michael Conlon
178 Purification and Assessment of Quality
of Radioiodinated Protein..................................................................1743
Graham S. Bailey
179 Purification of IgG by Precipitation with Sodium
Sulfate or Ammonium Sulfate...........................................................1749
Mark Page and Robin Thorpe
180 Purification of IgG Using Caprylic Acid.............................................1753
Mark Page and Robin Thorpe
181 Purification of IgG Using DEAE-Sepharose
Chromatography...............................................................................1755
Mark Page and Robin Thorpe
182 Purification of IgG Using Ion-Exchange HPLC.................................1757
Carl Dolman, Mark Page, and Robin Thorpe
183 Purification of IgG by Precipitation
with Polyethylene Glycol (PEG)........................................................1759
Mark Page and Robin Thorpe
184 Purification of IgG Using Protein A or Protein G...............................1761
Mark Page and Robin Thorpe
185 Analysis and Purification of IgG Using Size-Exclusion
High Performance Liquid Chromatography (SE-HPLC)....................1765
Carl Dolman and Robin Thorpe
186 Purification of IgG Using Affinity
Chromatography on Antigen-Ligand Columns..................................1769
Mark Page and Robin Thorpe
187 Purification of IgG Using Thiophilic Chromatography.......................1773
Mark Page and Robin Thorpe
188 Analysis of IgG Fractions by Electrophoresis....................................1775
Mark Page and Robin Thorpe
Contents xxi
189 Purification of Immunoglobulin Y (IgY)
from Chicken Eggs............................................................................1779
Christopher R. Bird and Robin Thorpe
190 Affinity Purification of Immunoglobulins
Using Protein A Mimetic (RAM).........................................................1783
Giorgio Fassina, Giovanna Palombo, Antonio Verdoliva,
and Menotti Ruvo
191 Detection of Serological Cross-Reactions
by Western Cross-Blotting................................................................1799
Peter Hammer!, Arnulf Hartl, Johannes Freund,
and Josef Thalhamer
192 Enzymatic Digestion of Monoclonal Antibodies................................1811
Sarah M. Andrew
193 How to Make Bispecific Antibodies...................................................1819
Ruth R. French
194 Antigen Measurement Using ELISA.................................................1827
William Jordan
195 Enhanced Chemiluminescence Immunoassay.................................1835
Richard A. W.Stott
196 Immunoprecipitation and Blotting:
The Visualization of Small Amounts of Antigens
Using Antibodies and Lectins.............................................................1845
Stephen Thompson
197 Determination of Epitopes by Mass Spectrometry............................1859
Christine Hager-Braun and Kenneth B. Tomer
198 Immunogen Preparation and Immunization
Procedures for Rats and Mice..........................................................1873
Mark Page and Robin Thorpe
199 Making Hybridomas..........................................................................1877
Robert Burns
200 Growing Hybridomas........................................................................1887
Gary Entrican, Catherine Jepson, and David Deane
201 Mouse Hybridomas as an Entryway to Monoclonal
Antibody Design and Production.......................................................1901
Eugene Mechetner
202 Potential Pitfalls in Monoclonal Antibody
Development and Applications..........................................................1911
Eugene Mechetner
xx/7 Contents
203 Recombinant Antibody Expression and Purification.........................1929
Achim Knappik and Ralf Brundlers
204 Screening Hybridoma Culture Supernatants
Using Solid-Phase Radiobinding Assay............................................1945
Mark Page and Robin Thorpe
205 Screening Hybridoma Culture Supernatants Using ELISA...............1947
Mark Page and Robin Thorpe
206 Growth and Purification of Murine
Monoclonal Antibodies......................................................................1949
Mark Page and Robin Thorpe
207 Affinity Purification Techniques for Monoclonal
Antibodies.........................................................................................1951
Alexander Schwarz
208 A Rapid Method for Generating Large Numbers
of High-Affinity Monoclonal Antibodies
from a Single Mouse.........................................................................1961
Nguyen Thi Man and Glenn E. Morris
Index............................................................................................................1975
|
| adam_txt |
Titel: The protein protocols handbook
Autor: Walker, John M
Jahr: 2009
Contents
Preface.v
Contributors.xxiii
Part I Quantitation of Proteins
1 Protein Determination by UV Absorption.3
Alastair Aitken and Michèle P. Learmonth
2 The Lowry Method for Protein Quantitation.7
Jakob H. Waterborg
3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation.11
John M. Walker
4 The Bradford Method For Protein Quantitation.17
Nicholas J. Kruger
5 Ultrafast Protein Determinations Using Microwave
Enhancement.25
Robert E. Akins and Rocky S. Tuan
6 The Nitric Acid Method for Protein Estimation
in Biological Samples.35
Scott A. Boerner, Crescent R. Isham, Yean Kit Lee,
Jennifer D. Tibodeau, Scott H. Kaufmann, and Keith C. Bible
7 Quantitation of Tryptophan in Proteins.47
Alastair Aitken and Michèle P. Learmonth
8 Kinetic Silver Staining of Proteins.51
Douglas D. Root and Kuan Wang
9 Quantitation of Cellular Proteins by Flow Cytometry.55
Thomas D. Friedrich, F. Andrew Ray, Ralph L. Smith,
and John M. Lehman
10 Quantitation of Cellular Proteins by Laser
Scanning Cytometry.63
Thomas D. Friedrich, Ralph L. Smith, and John M. Lehman
VII
viii Contents
Part II Electrophoresis of Proteins and Peptides
and Dectection in Gels
11 Protein Solubility in Two-Dimensional Electrophoresis:
Basic Principles and Issues.73
Thierry Rabilloud
12 Mouse and Human Tissue Sample Preparation
for 2-D Electrophoresis.85
Claus Zabel and Joachim Klose
13 Plant Protein Sample Preparation for 2-DE.109
Sebastien Christian Carpentier, Rony Swennen,
and Bart Panis
14 Preparation of Bacterial Samples for 2-D PAGE.121
Brian B, Vandahl, Gunna Christiansen, and Svend Birkelund
15 Preparation of Bodily Fluids for 2-D PAGE.131
Anthony G. Sullivan, Henry Brzeski, Jeban Ganesalingam,
and Manuel Mayr
16 Immunoaffinity Depletion of High Abundance
Plasma and Serum Proteins.139
Lynn A. Echan and David W. Speicher
17 Preparation of Yeast Samples for 2-D PAGE.155
Joakim Norbeck
18 Membrane Protein Preparation Using Aqueous Polymer
Two-Phase Systems.159
Jens Schindler and Hans Gerd Nothwang
19 Subcellular Fractionation of Small Sample Amounts.165
Hans Gerd Nothwang, Isabelle Guillemin,
and Jens Schindler
20 Nondenaturing Polyacrylamide Gel
Electrophoresis of Proteins.171
John M. Walker
21 SDS Polyacrylamide Gel Electrophoresis of Proteins.177
John M. Walker
22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins.187
John M. Walker
23 SDS-Polyacrylamide Gel Electrophoresis of Peptides.193
Ralph C. Judd
24 Separation of Proteins by Blue Native
Electrophoresis (BN-PAGE).201
Elke A. Dian, Joachim Rassow, and Christian Motz
Contents ix
25 Separation of Proteins by Gel Electrophoresis
in the Tris-Taurine-HCI System.211
Sylvie Luche, Mireille Chevallet, Cécile Lelong,
and Thierry Rabilloud
26 Cetyltrimethylammonium Bromide Discontinuous
Gel Electrophoresis of Proteins: M-Based Separation
of Proteins with Retained Native Activity.221
Robert E. Akins and Rocky S. Tuan
27 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis
of Basic Proteins.239
Jakob H. Waterborg
28 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis
of Histones.251
Jakob H. Waterborg
29 Isoelectric Focusing of Proteins in Ultra-Thin
Polyacrylamide Gels.:.263
John M. Walker
30 Serial Immobilized pH Gradient Isoelectric
Focusing over pH 4-9.269
Slobodan Poznanovic, Wojciech Wozny,
Helmut Zengerling, Gerhard P. Schwall,
and Michael A. Cahill
31 Radiolabeling of Eukaryotic Cells and Subsequent
Preparation for 2-Dimensional Electrophoresis.279
Nick Bizlos
32 Two-Dimensional Polyacrylamide Gel Electrophoresis
of Proteins Using Carrier Ampholyte pH Gradients
in the First Dimension.285
Patricia Gravel
33 Vertical Agarose Electrophoresis and Electroblotting
of High-Molecular-Weight Proteins.293
Marion L. Greaser and Chad M. Warren
34 Two-Dimensional PAGE of High-Molecular-Mass Proteins.299
Masamichi Oh-lshi and Tadakazu Maeda
35 Casting Immobilized pH Gradients.305
Elisabetta Gianazza
36 A Modified Nonequilibrium pH Gradient Electrophoresis
(NEPHGE) Technique for Protein Separation.323
James A. Carroll
x Contents
37 Microchip Capillary Electrophoresis:
Application to Peptide Analysis.329
Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte
38 Protein Separations in Microfluidic Chips.361
Andrea W. Chow and Bahram Fathollahi
39 Difference Gel Electrophoresis (DIGE).379
David B. Friedman and Kathryn S. Lilley
40 Comparing 2D Electrophoretic Gels Across
Internet Databases: An Open Source Application.409
Peter F. Lemkin, Gregory C. Thornwall, and Jai A. Evans
41 Quantification of Radiolabeled Proteins
in Polyacrylamide Gels.443
Wayne R. Springer
42 Differential ProteoTope Radioactive Quantification
of Protein Abundance Ratios.449
Wojciech Wozny, Gerhard P. Schwall, Chaturvedula S. Sastri,
Slobodan Poznanovic,Werner Stegmann, Christian Hunzinger*,
Karlfried Groebe, and Michael A. Cahill
43 Quantification of Proteins on Polyacrylamide Gels.479
Bryan John Smith
44 Using SDS-PAGE and Scanning Laser Densitometry
to Measure Yield and Degradation of Proteins.487
Aaron P. Miles and Allan Saul
45 Rapid and Sensitive Staining of Unfixed Proteins
in Polyacrylamide Gels with Nile Red.497
Joan-Ramon Daban, Salvador Bartolomé,
Antonio Bermúdez, and F. Javier Alba
46 Zn2+-Reverse Staining Technique.505
Carlos Fernandez-Patron
47 Protein Staining with Calconcarboxylic
Acid in Polyacrylamide Gels.515
Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin,
and Jung-Kap Choi
48 Detection of Proteins in Polyacrylamide Gels
by Silver Staining.527
Michael J. Dunn
49 Background-Free Protein Detection in Polyacrylamide
Gels and on Electroblots Using Transition Metal
Chelate Stains.529
Wayne F. Patton
Contents xi
50 Detection of Proteins in Polyacrylamide Gels
by Fluorescent Staining.547
Michael J. Dunn
51 Detection of Glycoproteins in Gels and Blots.555
Nicolle H. Packer, Malcolm S. Ball, Peter L. Devine,
and Wayne F. Patton
52 Staining of Glycoproteins/Proteoglycans on SDS-Gels.569
Holger J. Moller and Jörgen H. Poulsen
53 Detection of Proteins and Sialoglycoproteins
in Polyacrylamide Gels Using Eosin Y Stain.575
Fan Lin and Gary E. Wise
54 A Modified Pro-Q Diamond Staining Protocol
for Phosphoprotein Detection in Polyacrylamide Gels.579
Ganesh Kumar Agrawal and Jay J. Thelen
55 Electroelution of Proteins from Polyacrylamide Gels.587
Paul Jenö and Martin Horst
56 Autoradiography and Fluorography of Acrylamide Gels.595
Antonella Circolo and Sunita Gulati
57 Proteolytic Activity Detection by Two-Dimensional
Zymography.605
Jeff Wilkesman
Part III Blotting and Detection Methods
58 Protein Blotting by Electroblotting.617
Mark Page and Robin Thorpe
59 Protein Blotting by the Semidry Method.621
Patricia Gravel
60 Protein Blotting by the Capillary Method.631
John M. Walker
61 Western Blotting of Basic Proteins Electrophoretically
Resolved on Acid-Urea-Triton-Polyacrylamide Gels.635
Geneviève P. Delcuve and James R. Davie
62 Immunoblotting of 2-DE Separated Proteins.641
Barbara Magi and Laura Bianchi
63 High-Efficiency Blotting of High-Molecular Weight Proteins.663
Marion L. Greaser and Darl R. Swartz
64 Alkaline Phosphatase Labeling of IgG Antibody.673
G. Brian Wisdom
xii Contents
65 ß-Galactosidase Labeling of IgG Antibody.677
G. Brian Wisdom
66 Horseradish Peroxidase Labeling of IgG Antibody.681
G. Brian Wisdom
67 Digoxigenin Labeling of IgG Antibody.685
G. Brian Wisdom
68 Conjugation of Fluorochromes to Antibodies.687
Su-Yau Mao
69 Coupling of Antibodies with Biotin.693
Rosaria P. Haugland and Wendy W. You
70 Preparation of Avidin Conjugates.705
Rosaría P. Haugland and Mahesh K. Bhalgat
71 MDPF Staining of Proteins on Western Blots.717
F. Javier Alba and Joan-Ramon Daban
72. Copper Iodide Staining of Proteins and Its
Silver Enhancement.723
Douglas D. Root and Kuan Wang
73 Detection of Proteins on Blots Using Direct Blue 71.729
Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin, and Jung-Kap Choi
74 Detection of Proteins on Western Blots Using
Colorimetrie and Radiometric Visualization
of Secondary Ligands.737
Nicholas J. Kruger
75 Identification of Glycoproteins on Nitrocellulose
Membranes Using Lectin Blotting.755
Patricia Gravel
76 A Sensitive Method to Quantitatively Detect Total Protein
on Membranes after Electrophoretic Transfer Using
Avidin-or Streptavidin-Biotin.771
William J. LaRochelle
77 Detection and Quantification of Proteins on Immunoblots
using Enhanced Chemiluminescence.779
Jennifer J. Young
78 Reutilization of Western Blots After Chemiluminescent
or Autoradiographic Detection.789
Scott H. Kaufmann
79 The Use of Quantum Dot Luminescent Probes
for Western Blot Analysis.807
Savvas C. Makrides, Christina Gasbarro, and Job M. Bello
Contents xiii
80 The Use of Infrared Fluorescent Dyes
in Quantitative Immunoblotting.879
Ching-Hui Yang, Christopher Kasbek, and Harold A. Fisk
81 The Use of Infrared Fluorescent Dyes
in Immunofluorescence Microscopy.827
Christopher Kasbek, Ching-Hui Yang, and Harold A. Fisk
Part IV Chemical Modification of Proteins and Peptide
Production and Purification
82 Carboxymethylation of Cysteine
Using lodoacetamide/lodoacetic Acid.837
Alastair Aitken and Michèle Learmonth
83 Performic Acid Oxidation.841
Alastair Aitken and Michèle Learmonth
84 Succinylation of Proteins.845
Alastair Aitken and Michèle Learmonth
85 Pyridylethylation of Cysteine Residues.847
Malcolm Ward
86 Side Chain Selective Chemical Modifications of Proteins.851
Dan S. Tawfik
87 Nitration of Tyrosines.855
Dan S. Tawfik
88 Ethoxyformylation of Histidine.859
Dan S. Tawfik
89 Modification of Arginine Side Chains
with p-Hydroxyphenylglyoxal.863
Dan S. Tawfik
90 Amidation of Carboxyl Groups.865
Dan S. Tawfik
91 Amidination of Lysine Side Chains.867
Dan S. Tawfik
92 Modification of Tryptophan with 2-Hydroxy-
5-Nitrobenzylbromide.871
Dan S. Tawfik
93 Modification of Sulfhydryl Groups with DTNB.873
Dan S. Tawfik
94 Chemical Cleavage of Proteins at Methionyl-X
Peptide Bonds.875
Bryan John Smith
xiv Contents
95 Chemical Cleavage of Proteins at Tryptophanyl-X
Peptide Bonds.883
Bryan John Smith
96 Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds.891
Bryan John Smith
97 Chemical Cleavage of Proteins at Cysteinyl-X
Peptide Bonds.895
Bryan John Smith
98 Chemical Cleavage of Proteins at Asparaginyl-Glycyl
Peptide Bonds.899
Bryan John Smith
99 Enzymatic Digestion of Proteins in Solution
and in SDS Polyacrylamide Gels.905
Kathryn L. Stone, Eroi E. Gulcicek, and Kenneth R. Williams
100 On-PVDF Protein Digestions for N-terminal Sequencing
and Peptide Mass Fingerprinting.919
Victoria Pham, William Henzel, and Jennie R. Lili
101 Enzymatic Digestion of Membrane-Bound Proteins
for Peptide Mapping and Internal Sequence Analysis.927
Joseph Fernandez and Sheenah M. Mische
102 Reverse-Phase HPLC Separation of Enzymatic
Digests of Proteins.941
Kathryn L. Stone and Kenneth R. Williams
103 Peptide Mapping by Two-Dimensional Thin-Layer
Electrophoresis-Thin-Layer Chromatography.951
Ralph C. Judd
104 Peptide Mapping by Sodium Dodecyl
Sulfate-Polyacrylamide Gel Electrophoresis.963
Ralph C. Judd
105 Peptide Mapping for Protein Characterization.969
Peter Hojrup
106 Production of Protein Hydrolysates Using Enzymes.989
John M. Walker and Patricia J. Sweeney
107 Amino Acid Analysis by Precolumn Derivatization
with 1-Fluoro-2,4-Dinitrophenyl-5-i-Alanine Amide
(Marfey's Reagent).995
Sunil Kochhar and Philipp Christen
108 Amino Acid Analysis of Protein Hydrolysates Using Anion
Exchange Chromatography and IPAD Detection.1001
Petr Jandik, Jun Cheng, and Nebojsa Avdalovic
Contents xv
109 Validation of Amino Acid Analysis Methods.1015
Andrew J. Reason
110 Molecular Weight Estimation for Native Proteins
Using High-Performance Size-Exclusion
Chromatography.1029
G. Brent Irvine
111 Detection of Disulfide-Linked Peptides by HPLC.1039
Alastair Aitken and Michèle Learmonth
112 Detection of Disulfide-Linked Peptides by Mass
Spectrometry.1043
Alastair Aitken and Michèle Learmonth
113 Diagonal Electrophoresis for Detecting Disulfide Bridges.1047
Alastair Aitken and Michèle Learmonth
114 Estimation of Disulfide Bonds Using Ellman's Reagent.1053
Alastair Aitken and Michèle Learmonth
115 Quantitation of Cysteine Residues and Disulfide
Bonds by Electrophoresis.1057
Alastair Aitken and Michèle Learmonth
116 N-Terminal Sequencing of N-Terminally
Modified Proteins.1063
Roza Maria Kamp and Hisashi Hirano
117 Deblocking of Proteins Containing N-Terminal
Pyroglutamic Acid.1075
Jacek Mozdzanowski
118 Detection and Characterization of Protein
Mutations by Mass Spectrometry.1081
Yoshinao Wada
119 Peptide Sequencing by Nanoelectrospray
Tandem Mass Spectrometry.1095
Ole Norregaard Jensen and Matthias Wilm
120 Protein Identification by Peptide Mass Fingerprinting
using MALDI-TOF Mass Spectrometry.1117
Judith Webster and David Oxley
121 Protein Ladder Sequencing.1131
Rong Wang and Brian T. Chait
122 Sequence Analysis with WinGene/WinPep.1141
Lars Hennig
123 HPLC and Mass Spectrometry of Integral
Membrane Proteins.1149
Julian P. Whitelegge
xvi Contents
124 Enrichment of Serum Peptides and Analysis
by MALDI-TOF Mass Spectrometry.1167
YanmingAn, Habtom W. Ressom, and Radoslav Goldman
125 Computational Methods for Analysis of MALDI-TOF
Spectra to Discover Peptide Serum Biomarkers.1175
Habtom W. Ressom, Rency S. Varghese,
and Radoslav Goldman
Part V Posttranslational Modifications
126 Simple Tools for Complex A/-Glycan Analysis.1187
Anne-Catherine Fitchette, Meriem Benchabane,
Thomas Paccalet, Loïc Faye, and Véronique Gomord
127 A Lectin-Binding Assay for the Rapid Characterization
of the Glycosylation of Purified Glycoproteins.1205
Mohammad T. Goodarzi, Angeliki Fotinopoulou,
and Graham A. Turner
128 Chemical Methods of Analysis of Glycoproteins.1215
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
129 Monosaccharide Analysis by HPAEC.1219
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
130 Monosaccharide Analysis by Gas Chromatography (GC).1223
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
131 Determination of Monosaccharide Linkage and Substitution
Patterns by GC-MS Methylation Analysis.1227
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
132 Sialic Acid Analysis by HPAEC-PAD.1231
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
133 Chemical Release of O-Linked Oligosaccharide Chains.1233
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
134 O-Linked Oligosaccharide Profiling by HPLC.1235
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
135 O-Linked Oligosaccharide Profiling by HPAEC-PAD.1237
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
Contents xvii
136 Release of A/-Linked Oligosaccharide Chains
by Hydrazinolysis.1239
Tsuguo Mizuochi and Elizabeth F. Hounsell
137 Enzymatic Release of O-and A/-Linked
Oligosaccharide Chains.1243
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
138 A/-Linked Oligosaccharide Profiling by H PLC
on Porous Graphitized Carbon (PGC).1247
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
139 A/-Linked Oligosaccharide Profiling by HPAEC-PAD.1249
Elizabeth F. Hounsell, Michael J. Davies,
and Kevin D. Smith
140 HPAEC-PAD Analysis of Monosaccharides Released
by Exoglycosidase Digestion Using the CarboPac
MA1 Column.1253
Michael Weitzhandler, Jeffrey Rohrer, James R. Thayer,
and Nebojsa Avdalovic
141 Microassay Analyses of Protein Glycosylation.1261
Nicky K. C. Wong, Nnennaya Kanu, Natasha Thandrayen,
Geert Jan Rademaker, Christopher I. Baldwin,
David V. Renouf, and Elizabeth F. Hounsell
142 Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled
Carbohydrates from Glycoproteins.1273
Brian K. Brandley, John C. Klock, and Christopher M. Starr
143 HPLC Analysis of Fluorescently Labeled Glycans.1289
Tony Merry and Sviatlana Astrautsova
144 Glycoprofiling Purified Glycoproteins
Using Surface Plasmon Resonance.1313
Angeliki Fotinopoulou and Graham A. Turner
145 Sequencing Heparan Sulfate Saccharides.1321
Jeremy E. Turnbull
146 Analysis of Glycoprotein Heterogeneity by Capillary
Electrophoresis and Mass Spectrometry.1335
Andrew D. Hooker and David C. James
147 Affinity Chromatography of Oligosaccharides
and Glycopeptides with Immobilized Lectins.1347
Kazuo Yamamoto
148 In-Gel Enzymatic Release of W-Glycans.1363
David J. Harvey
xviii Contents
149 Analysis of /V-ünked Glycans by Mass Spectrometry.1371
David J. Harvey
150 MS Analysis of Protein Glycosylation.1387
NobuakiTakemori, Naoka Komori,
and Hiroyuki Matsumoto
151 Mapping protein A/-Glycosylation by COFRADIC.1395
Bart Ghesquière, Joël Vandekerckhove, and Kris Gevaert
152 Mass Spectrometric Analysis of O-Linked
Glycans Released Directly from Glycoproteins
in Gels Using ß-Elimination.1403
Adrian M. Taylor and Jane Thomas-Oates
153 Glycopeptide Analysis Using LC/MS and LC/MSn:
Site-Specific Glycosylation Analysis of a Glycoprotein.7479
Satsuki Itoh, Daisuke Takakura, Nana Kawasaki,
and Teruhide Yamaguchi
154 Identification of Vitamin K-Dependent Proteins
Using a Gla-Specific Monoclonal Antibody.1431
Karin Hansson
155 The Identification of Protein S-Nitrosocysteine.1451
Todd M. Greco, Sheryl L. Stamer, Daniel C. Liebler,
and Harry Ischiropoulos
156 Detection of Nitrotyrosine-Containing Proteins.1467
Xianquan Zhan and Dominic M. Desiderio
157 Mass Spectrometric Determination of Protein
Ubiquitination.1491
Carol E. Parker, Maria Warren Hines, Viorel Mocanu,
Susanna F. Greer, and Christoph H. Borchers
158 Detection of Sumoylated Proteins.1519
Ok-Kyong Park-Sarge and Kevin D. Sarge
159 Efficient Enrichment of Intact Phosphorylated Proteins
by Modified Immobilized Metal-Affinity
Chromatography.1531
Anna Dubrovska
160 Analyzing Protein Phosphorylation.1547
John Colyer
161 Mass Spectrometric Analysis of Protein Phosphorylation.1555
Stefan Gander, Alessio Cremonesi, Johana Chicher,
Suzette Moes, and Paul Jenö
Contents xix
162 Protein Microarrays for Phosphorylation Studies.1567
Birgit Kersten and Tanja Feilner
163 Two-Dimensional Phosphopeptide Mapping.1579
Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky,
and Steven F. Dowdy
164 Identification of Proteins Modified by Protein
(D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase.1589
Darin J. Weber and Philip N. McFadden
165 Analysis of Tyrosine-O-Sulfation.1601
Jens R. Bundgaard, Jette W. Sen, Anders H. Johnsen,
and Jens F. Reh feld
166 Analysis of Protein Palmitoylation
by Metabolic Radiolabeling Methods.1623
Katherine H. Pedone, Leah S. Bernstein, Maurine E. Under,
and John R. Hepler
167 Incorporation of Radiolabeled Prenyl Alcohols
and Their Analogs into Mammalian Cell Proteins:
A Useful Tool for Studying Proteins Prenylation.1637
Alberto Corsini, Christopher C. Farnsworth,
Paul McGeady, Michael H. Gelb, and John A. Glomset
168 The Metabolic Labeling and Analysis
of Isoprenylated Proteins.1657
Douglas A. Andres, Dean C. Crick, H. Peter Spielmann,
and Charles J. Waechter
Part VI Antibody Techniques
169 Antibody Production.1679
Robert Burns
170 Production of Antibodies Using Proteins
in Gel Bands.1687
Sally Ann Amero, Tharappel C. James,
and Sarah C. R. Elgin
171 Raising Highly Specific Polyclonal Antibodies Using
Biocompatible Support-Bound Antigens.1693
Monique Diano and André Le Bivio
172 Production of Antisera Using Peptide Conjugates.7705
Thomas E. Adrian
173 Small-Molecule-Protein Conjugation Procedures.1715
Stephen Thompson
xx Contents
174 The Chloramine T Method for Radiolabeling Protein.1727
Graham S. Bailey
175 The Lactoperoxidase Method
for Radiolabeling Protein.1731
Graham S. Bailey
176 The Bolton and Hunter Method
for Radiolabeling Protein.1733
Graham S. Bailey
177 Preparation of 125l-Labeled Peptides and Proteins
with High Specific Activity Using IODO-GEN.1735
J. Michael Conlon
178 Purification and Assessment of Quality
of Radioiodinated Protein.1743
Graham S. Bailey
179 Purification of IgG by Precipitation with Sodium
Sulfate or Ammonium Sulfate.1749
Mark Page and Robin Thorpe
180 Purification of IgG Using Caprylic Acid.1753
Mark Page and Robin Thorpe
181 Purification of IgG Using DEAE-Sepharose
Chromatography.1755
Mark Page and Robin Thorpe
182 Purification of IgG Using Ion-Exchange HPLC.1757
Carl Dolman, Mark Page, and Robin Thorpe
183 Purification of IgG by Precipitation
with Polyethylene Glycol (PEG).1759
Mark Page and Robin Thorpe
184 Purification of IgG Using Protein A or Protein G.1761
Mark Page and Robin Thorpe
185 Analysis and Purification of IgG Using Size-Exclusion
High Performance Liquid Chromatography (SE-HPLC).1765
Carl Dolman and Robin Thorpe
186 Purification of IgG Using Affinity
Chromatography on Antigen-Ligand Columns.1769
Mark Page and Robin Thorpe
187 Purification of IgG Using Thiophilic Chromatography.1773
Mark Page and Robin Thorpe
188 Analysis of IgG Fractions by Electrophoresis.1775
Mark Page and Robin Thorpe
Contents xxi
189 Purification of Immunoglobulin Y (IgY)
from Chicken Eggs.1779
Christopher R. Bird and Robin Thorpe
190 Affinity Purification of Immunoglobulins
Using Protein A Mimetic (RAM).1783
Giorgio Fassina, Giovanna Palombo, Antonio Verdoliva,
and Menotti Ruvo
191 Detection of Serological Cross-Reactions
by Western Cross-Blotting.1799
Peter Hammer!, Arnulf Hartl, Johannes Freund,
and Josef Thalhamer
192 Enzymatic Digestion of Monoclonal Antibodies.1811
Sarah M. Andrew
193 How to Make Bispecific Antibodies.1819
Ruth R. French
194 Antigen Measurement Using ELISA.1827
William Jordan
195 Enhanced Chemiluminescence Immunoassay.1835
Richard A. W.Stott
196 Immunoprecipitation and Blotting:
The Visualization of Small Amounts of Antigens
Using Antibodies and Lectins.1845
Stephen Thompson
197 Determination of Epitopes by Mass Spectrometry.1859
Christine Hager-Braun and Kenneth B. Tomer
198 Immunogen Preparation and Immunization
Procedures for Rats and Mice.1873
Mark Page and Robin Thorpe
199 Making Hybridomas.1877
Robert Burns
200 Growing Hybridomas.1887
Gary Entrican, Catherine Jepson, and David Deane
201 Mouse Hybridomas as an Entryway to Monoclonal
Antibody Design and Production.1901
Eugene Mechetner
202 Potential Pitfalls in Monoclonal Antibody
Development and Applications.1911
Eugene Mechetner
xx/7 Contents
203 Recombinant Antibody Expression and Purification.1929
Achim Knappik and Ralf Brundlers
204 Screening Hybridoma Culture Supernatants
Using Solid-Phase Radiobinding Assay.1945
Mark Page and Robin Thorpe
205 Screening Hybridoma Culture Supernatants Using ELISA.1947
Mark Page and Robin Thorpe
206 Growth and Purification of Murine
Monoclonal Antibodies.1949
Mark Page and Robin Thorpe
207 Affinity Purification Techniques for Monoclonal
Antibodies.1951
Alexander Schwarz
208 A Rapid Method for Generating Large Numbers
of High-Affinity Monoclonal Antibodies
from a Single Mouse.1961
Nguyen Thi Man and Glenn E. Morris
Index.1975 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author2 | Walker, John M. 1948- |
| author2_role | edt |
| author2_variant | j m w jm jmw |
| author_GND | (DE-588)137157754 |
| author_facet | Walker, John M. 1948- |
| building | Verbundindex |
| bvnumber | BV035069914 |
| callnumber-first | Q - Science |
| callnumber-label | QP551 |
| callnumber-raw | QP551 |
| callnumber-search | QP551 |
| callnumber-sort | QP 3551 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4170 WC 5150 WD 5100 |
| classification_tum | CHE 007f CHE 820f CHE 006f |
| ctrlnum | (OCoLC)191922694 (DE-599)BSZ284933783 |
| dewey-full | 572.6 |
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| discipline_str_mv | Biologie Chemie |
| edition | 3. ed. |
| format | Book |
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| id | DE-604.BV035069914 |
| illustrated | Illustrated |
| index_date | 2024-07-02T22:03:53Z |
| indexdate | 2024-07-09T21:21:30Z |
| institution | BVB |
| isbn | 160327474x 9781603274746 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016738392 |
| oclc_num | 191922694 |
| open_access_boolean | |
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| owner_facet | DE-703 DE-91G DE-BY-TUM DE-20 DE-19 DE-BY-UBM DE-11 DE-91S DE-BY-TUM |
| physical | XXXV, 1985 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Springer protocols handbooks |
| spelling | The protein protocols handbook ed. by John M. Walker 3. ed. New York, NY Humana Press 2009 XXXV, 1985 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer protocols handbooks Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Analyse (DE-588)4122795-5 gnd rswk-swf Proteine (DE-588)4076388-2 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Analyse (DE-588)4122795-5 s 1\p DE-604 Walker, John M. 1948- (DE-588)137157754 edt Erscheint auch als Online-Ausgabe 978-1-59745-198-7 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016738392&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | The protein protocols handbook Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Proteine (DE-588)4076388-2 gnd Analyse (DE-588)4122795-5 gnd |
| subject_GND | (DE-588)4255721-5 (DE-588)4076388-2 (DE-588)4122795-5 |
| title | The protein protocols handbook |
| title_auth | The protein protocols handbook |
| title_exact_search | The protein protocols handbook |
| title_exact_search_txtP | The protein protocols handbook |
| title_full | The protein protocols handbook ed. by John M. Walker |
| title_fullStr | The protein protocols handbook ed. by John M. Walker |
| title_full_unstemmed | The protein protocols handbook ed. by John M. Walker |
| title_short | The protein protocols handbook |
| title_sort | the protein protocols handbook |
| topic | Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Proteine (DE-588)4076388-2 gnd Analyse (DE-588)4122795-5 gnd |
| topic_facet | Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse Proteine Analyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016738392&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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