Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Giessen
Laufersweiler
2008
|
| Edition: | 1. Aufl. |
| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-90366 Inhaltsverzeichnis |
| Item Description: | Inhalt u.a.: Prävalenz durch Zecken in München |
| Physical Description: | 147 S. graph. Darst., Kt. |
| ISBN: | 383595332X |
Staff View
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| 245 | 1 | 0 | |a Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany |c von Cornelia Hildegard Anita Silaghi |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Giessen |b Laufersweiler |c 2008 | |
| 300 | |a 147 S. |b graph. Darst., Kt. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Inhalt u.a.: Prävalenz durch Zecken in München | ||
| 502 | |a Zugl.: München, Univ., Diss., 2008 | ||
| 650 | 7 | |a Ixodes Ricinus |2 cabt | |
| 650 | 7 | |a Anaplasma Phagocytophilum |2 cabt | |
| 650 | 7 | |a Rickettsia Helvetica |2 cabt | |
| 650 | 7 | |a Rickettsia Monacensis |2 cabt | |
| 650 | 7 | |a Tickborne Diseases |2 cabt | |
| 650 | 7 | |a Spotted Fever |2 cabt | |
| 650 | 7 | |a Polymerase Chain Reaction |2 cabt | |
| 650 | 7 | |a DNA Sequencing |2 cabt | |
| 650 | 7 | |a Germany |2 cabt | |
| 651 | 4 | |a Deutschland | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 912 | |a ebook | ||
| 940 | 1 | |n by | |
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Record in the Search Index
| _version_ | 1804138005187788800 |
|---|---|
| adam_text | AUS DEM INSTITUT FUR VERGLEICHENDE TROPENMEDIZIN UND PARASITOLOGIE DER
TIERARZTLICHEN FAKULTAT DER LUDWIG MAXIMILIANS-UNIVERSITAT MUNCHEN
VORSTAND: UNIV.-PROF. DR. KURT PFISTER ARBEIT ANGEFERTIGT UNTER LEITUNG
VON DR. JEREMIE GILLES PREVALENCE AND GENETIC ANALYSIS OI ANAPLASMA
PHAGOCYTOPHILUM AND SPOTTED FEVER GROUP RICKETTSIAE IN THE TICK IXODES
RICINUS IN URBAN AND PERIURBAN SITES IN SOUTHERN GERMANY INAUGURAL
-DISSERTATION ZUR ERLANGUNG DER TIERMEDIZINISCHEN DOKTORWURDE (DR. MED
VET.) DER TIERARZTLICHEN FAKULTAT DER LUDWIG-MAXIMILIANS-UNIVERSITAT
MUNCHEN VON CORNELIA HILDEGARD ANITA SILAGHI GEB. SEMMLER AUS MUNCHEN
MUNCHEN 2008 TABLE OF CONTENTS TABLE OF CONTENTS 1. INTRODUCTION 8 2.
LITERATURE REVIEW 10 2.1. IXODES RICINUS 10 2.1.1. TAXONOMY 10 2.1.2.
DISTRIBUTION AND HABITAT 10 2.1.3. LIFE CYCLE 11 2.1.4. SEASONAL
ACTIVITY 11 2.1.5. DIAPAUSE 12 2.1.6. HOST QUESTING 12 2.1.7. FEEDING
BEHAVIOR 12 2.1.8. IXODES RICINUS AS A VECTOR 13 2.2. THE ORDER
RICKETTSIALES 1 4 2.3. ANAPLASMA PHAGOCYTOPHILUM 14 2.3.1. SYSTEMATICS
AND MORPHOLOGY 14 2.3.2. GENETIC DIVERSITY 15 2.3.3. VECTORS AND
TRANSMISSION 16 2.3.4. HOSTS AND RESERVOIRS 17 2.3.5. GEOGRAPHICAL
DISTRIBUTION OF ANAPLASMA PHAGOCYTOPHILUM 19 2.3.5.1. QUESTING TICKS IN
EUROPE 19 2.3.5.2. QUESTING TICKS IN NORTH AMERICA AND ASIA 21 2.3.6
SEROPREVALENCE 21 2.3.6.1. SEROPREVALENCE IN HUMANS 21 2.3.6.2.
SEROPREVALENCE IN ANIMALS 22 2.3.7. DIAGNOSTIC AND PHYLOGENETIC TOOLS 23
2.3.8. GRANULOCYTIC ANAPLASMOSIS 24 2.4. SPOTTED FEVER GROUP RICKETTSIAE
25 2.4.1. SYSTEMATICS AND MORPHOLOGY 25 2.4.2. VECTOR, TRANSMISSION AND
RESERVOIR 26 2.4.3. GEOGRAPHICAL DISTRIBUTION AND PREVALENCE IN IXODES
RICINUS AND OTHER IXODID TICKS 27 2.4.4. SEROPREVALENCE 29 2.4.5.
DIAGNOSTIC AND PHYLOGENETIC TOOLS 29 2.4.6. TICK-BORNE RICKETTSIOSES IN
EUROPE 30 TABLE OF CONTENTS 2.5. POLYMERASE CHAIN REACTION 31 2.5.1.
PRINCIPLE OF POLYMERASE CHAIN REACTION 31 2.5.2. NESTED PCR 32 2.5.3.
REAL-TIME PCR 32 3. MATERIALS AND METHODS 34 3.1. TICK COLLECTION 34
3.1.1. SAMPLING METHOD-. 34 3.1.2. STUDY AREA 34 3.1.2.1. CITY PARKS 34
3.1.2.2. RIPARIAN FORESTS 36 3.1.2.3. SITES OUTSIDE OF MUNICH 38 3.2.
IDENTIFICATION OF TICKS AND DNA-EXTRACTION 39 3.3. QUALITY CONTROL OF
EXTRACTION AND QUANTIZATION OF DNA 39 3.4. POLYMERASE CHAIN REACTION FOR
DETECTION OF ANAPLASMA PHAGOCYTOPHILUM DNA 39 3.4.1. REAL-TIME PCR FOR
DETECTION OF THE MSP2 GENE OF ANAPLASMA PHAGOCYTOPHILUM 39 3.4.2. NESTED
PCR FOR DETECTION OF THE 16S RRNA GENE OF ANAPLASMA PHAGOCYTOPHILUM 41
3.5. POLYMERASE CHAIN REACTION FOR DETECTION OF RICKETTSIA SPP. DNA 43
3.5.1. PCR FOR THE DETECTION OF THE GLTA AND OMPA GENES 43 3.5.2. PCR
FOR THE DETECTION OF THE I6S RRNA AND OMPB GENES OF RICKETTSIA SPP 44
3.6. AGAROSE GEL ELECTROPHORESIS 45 3.7. DNA PURIFICATION 45 3.8.
SEQUENCING AND SEQUENCE ANALYSIS 46 3.9. STATISTICAL ANALYSIS 46 4.
RESULTS 47 4.1. TICK COLLECTION 47 4.2. NANODROP 48 4.3. REAL-TIME PCR
49 TABLE OF CONTENTS 4.4. PUBLICATIONS 50 4.4.1. PUBLICATION 1 51 4.4.2.
PUBLICATION 2 60 5. DISCUSSION 83 6. CONCLUSION 89 7. SUMMARY 90 8.
ZUSAMMENFASSUNG 91 9. REFERENCES 93 10. ABBREVIATIONS 122 11. FIGURES
124 12. TABLES 125 13. ANNEX 127 13.1. MATERIALS 127 13.1.1. KITS,
OLIGONUCLEOTIDS AND CHEMICALS 127 13.1.2. ENZYMES 128 13.1.3. BUFFER AND
SOLUTION FOR THE AGAROSE GEL ELECTROPHORESIS 128 13.2. SEQUENCING DATA
129 13.2.1. GLTA SEQUENCE COMPARISON OF RICKETTSIA HELVETICA 129 13.2.2.
GLTA SEQUENCE COMPARISON OF THE RICKETTSIA MONACENSIS CLUSTER 130
13.2.3. OMPA SEQUENCE COMPARISON OF THE RICKETTSIA MONACENSIS CLUSTER
132 13.2.4. OMPB SEQUENCE COMPARISON OF THE RICKETTSIA MONACENSIS
CLUSTER 135 13.3. STATISTIC FIGURES AND TABLES 139 14. ACKNOWLEDGMENTS
146
|
| adam_txt |
AUS DEM INSTITUT FUR VERGLEICHENDE TROPENMEDIZIN UND PARASITOLOGIE DER
TIERARZTLICHEN FAKULTAT DER LUDWIG MAXIMILIANS-UNIVERSITAT MUNCHEN
VORSTAND: UNIV.-PROF. DR. KURT PFISTER ARBEIT ANGEFERTIGT UNTER LEITUNG
VON DR. JEREMIE GILLES PREVALENCE AND GENETIC ANALYSIS OI ANAPLASMA
PHAGOCYTOPHILUM AND SPOTTED FEVER GROUP RICKETTSIAE IN THE TICK IXODES
RICINUS IN URBAN AND PERIURBAN SITES IN SOUTHERN GERMANY INAUGURAL
-DISSERTATION ZUR ERLANGUNG DER TIERMEDIZINISCHEN DOKTORWURDE (DR. MED
VET.) DER TIERARZTLICHEN FAKULTAT DER LUDWIG-MAXIMILIANS-UNIVERSITAT
MUNCHEN VON CORNELIA HILDEGARD ANITA SILAGHI GEB. SEMMLER AUS MUNCHEN
MUNCHEN 2008 TABLE OF CONTENTS TABLE OF CONTENTS 1. INTRODUCTION 8 2.
LITERATURE REVIEW 10 2.1. IXODES RICINUS 10 2.1.1. TAXONOMY 10 2.1.2.
DISTRIBUTION AND HABITAT 10 2.1.3. LIFE CYCLE 11 2.1.4. SEASONAL
ACTIVITY 11 2.1.5. DIAPAUSE 12 2.1.6. HOST QUESTING 12 2.1.7. FEEDING
BEHAVIOR 12 2.1.8. IXODES RICINUS AS A VECTOR 13 2.2. THE ORDER
RICKETTSIALES 1 4 2.3. ANAPLASMA PHAGOCYTOPHILUM 14 2.3.1. SYSTEMATICS
AND MORPHOLOGY 14 2.3.2. GENETIC DIVERSITY 15 2.3.3. VECTORS AND
TRANSMISSION 16 2.3.4. HOSTS AND RESERVOIRS 17 2.3.5. GEOGRAPHICAL
DISTRIBUTION OF ANAPLASMA PHAGOCYTOPHILUM 19 2.3.5.1. QUESTING TICKS IN
EUROPE 19 2.3.5.2. QUESTING TICKS IN NORTH AMERICA AND ASIA 21 2.3.6
SEROPREVALENCE 21 2.3.6.1. SEROPREVALENCE IN HUMANS 21 2.3.6.2.
SEROPREVALENCE IN ANIMALS 22 2.3.7. DIAGNOSTIC AND PHYLOGENETIC TOOLS 23
2.3.8. GRANULOCYTIC ANAPLASMOSIS 24 2.4. SPOTTED FEVER GROUP RICKETTSIAE
25 2.4.1. SYSTEMATICS AND MORPHOLOGY 25 2.4.2. VECTOR, TRANSMISSION AND
RESERVOIR 26 2.4.3. GEOGRAPHICAL DISTRIBUTION AND PREVALENCE IN IXODES
RICINUS AND OTHER IXODID TICKS 27 2.4.4. SEROPREVALENCE 29 2.4.5.
DIAGNOSTIC AND PHYLOGENETIC TOOLS 29 2.4.6. TICK-BORNE RICKETTSIOSES IN
EUROPE 30 TABLE OF CONTENTS 2.5. POLYMERASE CHAIN REACTION 31 2.5.1.
PRINCIPLE OF POLYMERASE CHAIN REACTION 31 2.5.2. NESTED PCR 32 2.5.3.
REAL-TIME PCR 32 3. MATERIALS AND METHODS 34 3.1. TICK COLLECTION 34
3.1.1. SAMPLING METHOD-. 34 3.1.2. STUDY AREA 34 3.1.2.1. CITY PARKS 34
3.1.2.2. RIPARIAN FORESTS 36 3.1.2.3. SITES OUTSIDE OF MUNICH 38 3.2.
IDENTIFICATION OF TICKS AND DNA-EXTRACTION 39 3.3. QUALITY CONTROL OF
EXTRACTION AND QUANTIZATION OF DNA 39 3.4. POLYMERASE CHAIN REACTION FOR
DETECTION OF ANAPLASMA PHAGOCYTOPHILUM DNA 39 3.4.1. REAL-TIME PCR FOR
DETECTION OF THE MSP2 GENE OF ANAPLASMA PHAGOCYTOPHILUM 39 3.4.2. NESTED
PCR FOR DETECTION OF THE 16S RRNA GENE OF ANAPLASMA PHAGOCYTOPHILUM 41
3.5. POLYMERASE CHAIN REACTION FOR DETECTION OF RICKETTSIA SPP. DNA 43
3.5.1. PCR FOR THE DETECTION OF THE GLTA AND OMPA GENES 43 3.5.2. PCR
FOR THE DETECTION OF THE I6S RRNA AND OMPB GENES OF RICKETTSIA SPP 44
3.6. AGAROSE GEL ELECTROPHORESIS 45 3.7. DNA PURIFICATION 45 3.8.
SEQUENCING AND SEQUENCE ANALYSIS 46 3.9. STATISTICAL ANALYSIS 46 4.
RESULTS 47 4.1. TICK COLLECTION 47 4.2. NANODROP 48 4.3. REAL-TIME PCR
49 TABLE OF CONTENTS 4.4. PUBLICATIONS 50 4.4.1. PUBLICATION 1 51 4.4.2.
PUBLICATION 2 60 5. DISCUSSION 83 6. CONCLUSION 89 7. SUMMARY 90 8.
ZUSAMMENFASSUNG 91 9. REFERENCES 93 10. ABBREVIATIONS 122 11. FIGURES
124 12. TABLES 125 13. ANNEX 127 13.1. MATERIALS 127 13.1.1. KITS,
OLIGONUCLEOTIDS AND CHEMICALS 127 13.1.2. ENZYMES 128 13.1.3. BUFFER AND
SOLUTION FOR THE AGAROSE GEL ELECTROPHORESIS 128 13.2. SEQUENCING DATA
129 13.2.1. GLTA SEQUENCE COMPARISON OF RICKETTSIA HELVETICA 129 13.2.2.
GLTA SEQUENCE COMPARISON OF THE RICKETTSIA MONACENSIS CLUSTER 130
13.2.3. OMPA SEQUENCE COMPARISON OF THE RICKETTSIA MONACENSIS CLUSTER
132 13.2.4. OMPB SEQUENCE COMPARISON OF THE RICKETTSIA MONACENSIS
CLUSTER 135 13.3. STATISTIC FIGURES AND TABLES 139 14. ACKNOWLEDGMENTS
146 |
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| author | Silaghi, Cornelia Hildegard Anita 1975- |
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| dewey-full | 636.89 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.89 |
| dewey-search | 636.89 |
| dewey-sort | 3636.89 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| discipline_str_mv | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| id | DE-604.BV035060956 |
| illustrated | Illustrated |
| index_date | 2024-07-02T22:00:32Z |
| indexdate | 2024-07-09T21:21:18Z |
| institution | BVB |
| isbn | 383595332X |
| language | English |
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| physical | 147 S. graph. Darst., Kt. |
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| publishDate | 2008 |
| publishDateSearch | 2008 |
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| publisher | Laufersweiler |
| record_format | marc |
| spelling | Silaghi, Cornelia Hildegard Anita 1975- Verfasser (DE-588)137068093 aut Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany von Cornelia Hildegard Anita Silaghi 1. Aufl. Giessen Laufersweiler 2008 147 S. graph. Darst., Kt. txt rdacontent n rdamedia nc rdacarrier Inhalt u.a.: Prävalenz durch Zecken in München Zugl.: München, Univ., Diss., 2008 Ixodes Ricinus cabt Anaplasma Phagocytophilum cabt Rickettsia Helvetica cabt Rickettsia Monacensis cabt Tickborne Diseases cabt Spotted Fever cabt Polymerase Chain Reaction cabt DNA Sequencing cabt Germany cabt Deutschland (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-90366 http://edoc.ub.uni-muenchen.de/9036/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-90366 Resolving-System HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016729465&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Silaghi, Cornelia Hildegard Anita 1975- Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany Ixodes Ricinus cabt Anaplasma Phagocytophilum cabt Rickettsia Helvetica cabt Rickettsia Monacensis cabt Tickborne Diseases cabt Spotted Fever cabt Polymerase Chain Reaction cabt DNA Sequencing cabt Germany cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany |
| title_auth | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany |
| title_exact_search | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany |
| title_exact_search_txtP | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany |
| title_full | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany von Cornelia Hildegard Anita Silaghi |
| title_fullStr | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany von Cornelia Hildegard Anita Silaghi |
| title_full_unstemmed | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany von Cornelia Hildegard Anita Silaghi |
| title_short | Prevalence and genetic analysis of "Anaplasma phagocytophilum" and spotted fever group rickettsiae in the tick "Ixodes ricinus" in urban and periurban sites in Southern Germany |
| title_sort | prevalence and genetic analysis of anaplasma phagocytophilum and spotted fever group rickettsiae in the tick ixodes ricinus in urban and periurban sites in southern germany |
| topic | Ixodes Ricinus cabt Anaplasma Phagocytophilum cabt Rickettsia Helvetica cabt Rickettsia Monacensis cabt Tickborne Diseases cabt Spotted Fever cabt Polymerase Chain Reaction cabt DNA Sequencing cabt Germany cabt |
| topic_facet | Ixodes Ricinus Anaplasma Phagocytophilum Rickettsia Helvetica Rickettsia Monacensis Tickborne Diseases Spotted Fever Polymerase Chain Reaction DNA Sequencing Germany Deutschland Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/9036/ https://nbn-resolving.org/urn:nbn:de:bvb:19-90366 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016729465&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT silaghicorneliahildegardanita prevalenceandgeneticanalysisofanaplasmaphagocytophilumandspottedfevergrouprickettsiaeinthetickixodesricinusinurbanandperiurbansitesinsoutherngermany |