RNA-protein interaction protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2008
|
| Edition: | 2. ed. |
| Series: | Methods in molecular biology
488 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 416 S. Ill., graph. Darst. |
| ISBN: | 9781588294197 9781603274753 |
Staff View
MARC
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| 245 | 1 | 0 | |a RNA-protein interaction protocols |c ed. by Ren-Jang Lin |
| 246 | 1 | 3 | |a RNA protein interaction protocols |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2008 | |
| 300 | |a XV, 416 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a RNA-Binding Proteins |x analysis |v Laboratory Manuals | |
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Record in the Search Index
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| adam_text | Contents
Preface
......................................................................................................
v
Contributors
.............................................................................................. xiii
1.
Isolation of a Sequence-Specific
RNA
Binding Protein,
Polypyrimidine Tract Binding Protein, Using
RNA
Affinity Chromatography
Shalini Sharma
................................................................................. 7
2
An Affinity Oligonucleotide Displacement Strategy to Purify
Ribonucleoprotein Complexes Applied to Human Telomerase
Isabel Kurth, Gael Cristofari, and Joachim Lingner
.......................... 9
3
RNA
Affinity Tags for the Rapid Purification and Investigation
of RNAs and RNA-Protein Complexes
Scott C. Walker, Felicia H. Scott, Chatchawan Srisawat,
and David R. Engelke
....................................................................... 23
4
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes
Containing Transcribed or Synthetic U6 snRNA
Kenneth J.
Dery,
Shyue-Lee Yean, and Ren-jang Lin
......................... 41
5
Purification of Ribonucleoproteins Using Peptide-Elutable
Antibodies and Other Affinity Techniques
Scott W. Stevens
............................................................................... 65
6
CLIP: Crosslinking and ImmunoPrecipitation of In Vivo
RNA
Targets of RNA-Binding Proteins
Kirk B. Jensen and Robert B. Darnell.
............................................... 85
7
Quantitative Analysis of
Protei
n-RNA Interactions
by Gel Mobility Shift
Sean P. Ryder, Michael l.
Recht,
and James R. Williamson
................ 99
8
Monitoring Assembly of Ribonucleoprotein Complexes
by Isothermal
Titration Calorimetry
Michael I.
Recht,
Sean P. Ryder, and James R. Williamson
................ 1
Г
7
9
Characterization of RNA-Protein Interactions by Phosphorothioate
Footprinting and Its Applications to the Ribosome
A.
Özlem Tastan
Bishop,
Ulrich Stelzl, Markus Pech,
and KnudH.
Nierhaus...................................................................... 129
10
In Vivo Analysis of Ribonucleoprotein Complexes Using
Nucleotide Analog Interference Mapping
Lara B.
Weinstein Szewczak............................................................. 153
IX
Contents
11 17
RNA
Polymerase-Mediated
Incorporation
of 8-N3AMP
Into
RNA
for Studying Protein-RNA Interactions
Rajesh K.Gaur
..................................................................................
12
A Simple Crosslinking Method, CLAMP, to Map the Sites
of RNA-Contacting Domains Within a Protein
Hiren Banerjee and Ravinder Singh
................................................. 181
13
Proteins Specifically Modified With a Chemical Nuclease
as Probes of RNA-Protein Interaction
Oliver A. Kent and Andrew M. MacMillan
........................................ 191
14
RNA-Protein Crosslink Mapping Using TEV Protease
Ian A. Turner, Chris M. Norman, Mark
/.
Churcher,
and Andrew J. Newman
....................................................................201
15
Structural Analysis of Protein-RNA Interactions With
Mass Spectrometry
Mamuka Kvaratskhelia and Stuart F.J.
Le Grice
................................213
16
Analyzing RNA-Protein Crosslinking Sites in Unlabeled
Ribonucleoprotein Complexes by Mass Spectrometry
Henning Urlaub, Eva Kuhn-Hölsken, and Reinhard Lührmann.........221
17 In Vitro
Selection of Random
RNA
Fragments to Identify
Protein-Binding Sites Within Large RNAs
Ulrich Stelzt
and Knud H.
Nierhaus.................................................247
18
Immunoprecipitation Analysis to Study RNA-Protein
Interactions in Xenopus Oocytes
Naoto Mabuchi, Kaoru Masuyama, and Mutsuhito Ohno
................257
19
Mapping the Regions of RNase
Ρ
Catalytic
RNA
That
Are Potentially in Close Contact With
Its Protein Cofactor
Phong Trang and Fenyong Liu
..........................................................267
20
Quantification of MicroRNAs, Splicing Isoforms,
and Homologous mRNAs With the Invader Assay
Peggy S.
Eis
and Mariano A. Garcia-Blanco
......................................279
21
Analysis of
RNA
Structure and RNA-Protein Interactions
in Mammalian Cells by Use of Terminal
Transferase-Dependent PCR
Hsiu-Hua Chen, Jeanne LeBon, and Arthur D.
Riggs
........................319
22
Duplex Unwinding and RNP Remodeling With
RNA Helicases
Eckhard
Jankowsky and Margaret E. Fairman
...................................343
23
Preparation of Efficient Splicing Extracts From Whole Ceils,
Nuclei, and Cytoplasmic Fractions
Naoyuki Kataoka and Gideon Dreyfuss
............................................357
Contents xi
24
Designing
and Utilization of siRNAs Targeting
RNA
Binding Proteins
Dong
-Но
Kim, Mark Behlke, and John J. Rossi
.................................367
25
The Use of Saccharomyces cerevisiae Proteomic Libraries to Identify
RNA-Modifying Proteins
Jane E.
fackman,
Elizabeth J. Grayhack, and Eric M. Phizicky
..........383
Index
.............................................................................................................395
|
| adam_txt |
Contents
Preface
.
v
Contributors
. xiii
1.
Isolation of a Sequence-Specific
RNA
Binding Protein,
Polypyrimidine Tract Binding Protein, Using
RNA
Affinity Chromatography
Shalini Sharma
. 7
2
An Affinity Oligonucleotide Displacement Strategy to Purify
Ribonucleoprotein Complexes Applied to Human Telomerase
Isabel Kurth, Gael Cristofari, and Joachim Lingner
. 9
3
RNA
Affinity Tags for the Rapid Purification and Investigation
of RNAs and RNA-Protein Complexes
Scott C. Walker, Felicia H. Scott, Chatchawan Srisawat,
and David R. Engelke
. 23
4
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes
Containing Transcribed or Synthetic U6 snRNA
Kenneth J.
Dery,
Shyue-Lee Yean, and Ren-jang Lin
. 41
5
Purification of Ribonucleoproteins Using Peptide-Elutable
Antibodies and Other Affinity Techniques
Scott W. Stevens
. 65
6
CLIP: Crosslinking and ImmunoPrecipitation of In Vivo
RNA
Targets of RNA-Binding Proteins
Kirk B. Jensen and Robert B. Darnell.
. 85
7
Quantitative Analysis of
Protei
n-RNA Interactions
by Gel Mobility Shift
Sean P. Ryder, Michael l.
Recht,
and James R. Williamson
. 99
8
Monitoring Assembly of Ribonucleoprotein Complexes
by Isothermal
Titration Calorimetry
Michael I.
Recht,
Sean P. Ryder, and James R. Williamson
. 1
Г
7
9
Characterization of RNA-Protein Interactions by Phosphorothioate
Footprinting and Its Applications to the Ribosome
A.
Özlem Tastan
Bishop,
Ulrich Stelzl, Markus Pech,
and KnudH.
Nierhaus. 129
10
In Vivo Analysis of Ribonucleoprotein Complexes Using
Nucleotide Analog Interference Mapping
Lara B.
Weinstein Szewczak. 153
IX
Contents
11 17
RNA
Polymerase-Mediated
Incorporation
of 8-N3AMP
Into
RNA
for Studying Protein-RNA Interactions
Rajesh K.Gaur
.
12
A Simple Crosslinking Method, CLAMP, to Map the Sites
of RNA-Contacting Domains Within a Protein
Hiren Banerjee and Ravinder Singh
. 181
13
Proteins Specifically Modified With a Chemical Nuclease
as Probes of RNA-Protein Interaction
Oliver A. Kent and Andrew M. MacMillan
. 191
14
RNA-Protein Crosslink Mapping Using TEV Protease
Ian A. Turner, Chris M. Norman, Mark
/.
Churcher,
and Andrew J. Newman
.201
15
Structural Analysis of Protein-RNA Interactions With
Mass Spectrometry
Mamuka Kvaratskhelia and Stuart F.J.
Le Grice
.213
16
Analyzing RNA-Protein Crosslinking Sites in Unlabeled
Ribonucleoprotein Complexes by Mass Spectrometry
Henning Urlaub, Eva Kuhn-Hölsken, and Reinhard Lührmann.221
17 In Vitro
Selection of Random
RNA
Fragments to Identify
Protein-Binding Sites Within Large RNAs
Ulrich Stelzt
and Knud H.
Nierhaus.247
18
Immunoprecipitation Analysis to Study RNA-Protein
Interactions in Xenopus Oocytes
Naoto Mabuchi, Kaoru Masuyama, and Mutsuhito Ohno
.257
19
Mapping the Regions of RNase
Ρ
Catalytic
RNA
That
Are Potentially in Close Contact With
Its Protein Cofactor
Phong Trang and Fenyong Liu
.267
20
Quantification of MicroRNAs, Splicing Isoforms,
and Homologous mRNAs With the Invader Assay
Peggy S.
Eis
and Mariano A. Garcia-Blanco
.279
21
Analysis of
RNA
Structure and RNA-Protein Interactions
in Mammalian Cells by Use of Terminal
Transferase-Dependent PCR
Hsiu-Hua Chen, Jeanne LeBon, and Arthur D.
Riggs
.319
22
Duplex Unwinding and RNP Remodeling With
RNA Helicases
Eckhard
Jankowsky and Margaret E. Fairman
.343
23
Preparation of Efficient Splicing Extracts From Whole Ceils,
Nuclei, and Cytoplasmic Fractions
Naoyuki Kataoka and Gideon Dreyfuss
.357
Contents xi
24
Designing
and Utilization of siRNAs Targeting
RNA
Binding Proteins
Dong
-Но
Kim, Mark Behlke, and John J. Rossi
.367
25
The Use of Saccharomyces cerevisiae Proteomic Libraries to Identify
RNA-Modifying Proteins
Jane E.
fackman,
Elizabeth J. Grayhack, and Eric M. Phizicky
.383
Index
.395 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T21:52:44Z |
| indexdate | 2024-07-09T21:20:48Z |
| institution | BVB |
| isbn | 9781588294197 9781603274753 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016708475 |
| oclc_num | 166378576 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-703 DE-20 DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-703 DE-20 DE-11 |
| physical | XV, 416 S. Ill., graph. Darst. |
| publishDate | 2008 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | RNA-protein interaction protocols ed. by Ren-Jang Lin RNA protein interaction protocols 2. ed. Totowa, NJ Humana Press 2008 XV, 416 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 488 Springer protocols ARN - composition chimique Interactions ARN-protéine ram Interactions ARN-protéines - Manuels de laboratoire Protéines de liaison de l'ARN Ribonucléoprotéine - analyse RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals Wechselwirkung (DE-588)4064937-4 gnd rswk-swf RNS-Bindungsproteine (DE-588)4178253-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS-Bindungsproteine (DE-588)4178253-7 s Wechselwirkung (DE-588)4064937-4 s b DE-604 Lin, Ren-Jang Sonstige oth Methods in molecular biology 488 (DE-604)BV035362695 488 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016708475&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RNA-protein interaction protocols Methods in molecular biology ARN - composition chimique Interactions ARN-protéine ram Interactions ARN-protéines - Manuels de laboratoire Protéines de liaison de l'ARN Ribonucléoprotéine - analyse RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals Wechselwirkung (DE-588)4064937-4 gnd RNS-Bindungsproteine (DE-588)4178253-7 gnd |
| subject_GND | (DE-588)4064937-4 (DE-588)4178253-7 (DE-588)4143413-4 |
| title | RNA-protein interaction protocols |
| title_alt | RNA protein interaction protocols |
| title_auth | RNA-protein interaction protocols |
| title_exact_search | RNA-protein interaction protocols |
| title_exact_search_txtP | RNA-protein interaction protocols |
| title_full | RNA-protein interaction protocols ed. by Ren-Jang Lin |
| title_fullStr | RNA-protein interaction protocols ed. by Ren-Jang Lin |
| title_full_unstemmed | RNA-protein interaction protocols ed. by Ren-Jang Lin |
| title_short | RNA-protein interaction protocols |
| title_sort | rna protein interaction protocols |
| topic | ARN - composition chimique Interactions ARN-protéine ram Interactions ARN-protéines - Manuels de laboratoire Protéines de liaison de l'ARN Ribonucléoprotéine - analyse RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals Wechselwirkung (DE-588)4064937-4 gnd RNS-Bindungsproteine (DE-588)4178253-7 gnd |
| topic_facet | ARN - composition chimique Interactions ARN-protéine Interactions ARN-protéines - Manuels de laboratoire Protéines de liaison de l'ARN Ribonucléoprotéine - analyse RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals Wechselwirkung RNS-Bindungsproteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016708475&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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