Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 259 S. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |c von Ute Neugebauer |
| 264 | 1 | |c 2007 | |
| 300 | |a VI, 259 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Jena, Univ., Diss., 2007 | ||
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Datensatz im Suchindex
| _version_ | 1804137955709681664 |
|---|---|
| adam_text | Contents
1 Introduction 1
2 Theoretical fundamentals 7
2.1 Vibrational Spectroscopy 7
2.2 Atomic force microscopy (AFM) 22
2.3 Statistical evaluation methods: Multivariate data analysis 24
3 Material and Methods 29
3.1 Spectroscopic instrumentation 29
3.2 Biological samples 38
3.3 Data treatment 46
3.4 Statistical data analysis 47
3.5 DFT calculation 50
4 Bacteria 53
4.1 Vibrational spectroscopic characterization 54
4.2 Vibrational spectroscopic discrimination of bacteria 62
4.3 Bacterial metabolism: Vibrational spectroscopic study of bacterial growth .... 63
4.4 Conclusion 73
5 Tip-enhanced Raman spectroscopy (TERS) of complex biological surfaces 75
5.1 Biological fundamentals: Cell wall structure of Gram-positive bacteria 75
5.2 TERS while scanning the bacterial surface with AFM 77
5.3 TERS on selected points on the sample surface 78
5.4 Dynamics on the bacterial cell surface probed by TERS 84
5.5 TERS of bacteria under special conditions: Cells under argon 85
5.6 Estimation of the enhancement factor 86
5.7 Possible problems 87
5.8 Summary and outlook 88
6 Fluoroquinolones - important antibiotics 91
6.1 Background and history 91
6.2 Structural characteristics of the fluoroquinolones 92
6.3 Proposed mode of action of the fluoroquinolones 94
6.4 UV/vis absorption spectra 96
6.5 DFT calculations 97
6.6 Raman spectra of the bulk substances 98
6.7 IR absorption spectra of the bulk substances 103
6.8 Raman spectra of the quinolones in hydrated environment 104
iii
Contents
6.9 Raman spectra in solution, pH-dependence 107
6.10 Surface-enhanced Raman spectra 108
6.11 Resonance Raman spectra 110
6.12 Conclusion Ill
7 The biological target: DNA 113
7.1 The DNA building blocks: nucleic acid bases, nucleoside and nucleotides .... 113
7.2 Primary and secondary structure of DNA 134
7.3 The plasmid-DNA pBR322 135
7.4 Spectroscopic characterization of DNA 135
7.5 Tertiary structure of DNA: supercoiling 141
7.6 Intercalation of ethidium bromide 145
7.7 Summary and outlook 150
8 The biological target: DNA-gyrase 151
8.1 Structure and biological function of the enzyme gyrase 151
8.2 The protein building blocks: the amino acids 154
8.3 Spectroscopic characterization of the enzyme 158
8.4 Interactions with DNA and the drug 160
8.5 Summary 164
9 Influence of fluoroquinolone drugs on bacterial growth 165
9.1 Minimal inhibition concentration 165
9.2 Effect of moxifloxacin on Staphylococcus epidermidis 166
9.3 Effect of ciprofloxacin on Bacilluspumilus 178
9.4 Summary and Conclusion 179
10 Summary and Outlook 181
11 Zusammenfassung und Ausblick 187
Appendix 193
A Chemicals and Media 195
A.I Antibiotics 195
A.2 Cellular Components 195
A.3 Bacteria 196
A.4 Buffer 197
A.5 Gel electrophoreses 200
A.6 Further biological assays 202
B Additional spectra and tables 203
B.I DNA and DNA building blocks 203
B.2 Gyrase and amino acids 218
Abbreviations 221
Bibliography 227
iv
Contents
Publication list 243
Conference Contributions 245
List of figures 252
List of tables 254
Danksagung 255
Selbstandigkeitserklarung 257
Lebenslauf 259
v
|
| adam_txt |
Contents
1 Introduction 1
2 Theoretical fundamentals 7
2.1 Vibrational Spectroscopy 7
2.2 Atomic force microscopy (AFM) 22
2.3 Statistical evaluation methods: Multivariate data analysis 24
3 Material and Methods 29
3.1 Spectroscopic instrumentation 29
3.2 Biological samples 38
3.3 Data treatment 46
3.4 Statistical data analysis 47
3.5 DFT calculation 50
4 Bacteria 53
4.1 Vibrational spectroscopic characterization 54
4.2 Vibrational spectroscopic discrimination of bacteria 62
4.3 Bacterial metabolism: Vibrational spectroscopic study of bacterial growth . 63
4.4 Conclusion 73
5 Tip-enhanced Raman spectroscopy (TERS) of complex biological surfaces 75
5.1 Biological fundamentals: Cell wall structure of Gram-positive bacteria 75
5.2 TERS while scanning the bacterial surface with AFM 77
5.3 TERS on selected points on the sample surface 78
5.4 Dynamics on the bacterial cell surface probed by TERS 84
5.5 TERS of bacteria under special conditions: Cells under argon 85
5.6 Estimation of the enhancement factor 86
5.7 Possible problems 87
5.8 Summary and outlook 88
6 Fluoroquinolones - important antibiotics 91
6.1 Background and history 91
6.2 Structural characteristics of the fluoroquinolones 92
6.3 Proposed mode of action of the fluoroquinolones 94
6.4 UV/vis absorption spectra 96
6.5 DFT calculations 97
6.6 Raman spectra of the bulk substances 98
6.7 IR absorption spectra of the bulk substances 103
6.8 Raman spectra of the quinolones in hydrated environment 104
iii
Contents
6.9 Raman spectra in solution, pH-dependence 107
6.10 Surface-enhanced Raman spectra 108
6.11 Resonance Raman spectra 110
6.12 Conclusion Ill
7 The biological target: DNA 113
7.1 The DNA building blocks: nucleic acid bases, nucleoside and nucleotides . 113
7.2 Primary and secondary structure of DNA 134
7.3 The plasmid-DNA pBR322 135
7.4 Spectroscopic characterization of DNA 135
7.5 Tertiary structure of DNA: supercoiling 141
7.6 Intercalation of ethidium bromide 145
7.7 Summary and outlook 150
8 The biological target: DNA-gyrase 151
8.1 Structure and biological function of the enzyme gyrase 151
8.2 The protein building blocks: the amino acids 154
8.3 Spectroscopic characterization of the enzyme 158
8.4 Interactions with DNA and the drug 160
8.5 Summary 164
9 Influence of fluoroquinolone drugs on bacterial growth 165
9.1 Minimal inhibition concentration 165
9.2 Effect of moxifloxacin on Staphylococcus epidermidis 166
9.3 Effect of ciprofloxacin on Bacilluspumilus 178
9.4 Summary and Conclusion 179
10 Summary and Outlook 181
11 Zusammenfassung und Ausblick 187
Appendix 193
A Chemicals and Media 195
A.I Antibiotics 195
A.2 Cellular Components 195
A.3 Bacteria 196
A.4 Buffer 197
A.5 Gel electrophoreses 200
A.6 Further biological assays 202
B Additional spectra and tables 203
B.I DNA and DNA building blocks 203
B.2 Gyrase and amino acids 218
Abbreviations 221
Bibliography 227
iv
Contents
Publication list 243
Conference Contributions 245
List of figures 252
List of tables 254
Danksagung 255
Selbstandigkeitserklarung 257
Lebenslauf 259
v |
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| author | Neugebauer, Ute 1978- |
| author_GND | (DE-588)13305456X |
| author_facet | Neugebauer, Ute 1978- |
| author_role | aut |
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| building | Verbundindex |
| bvnumber | BV035027042 |
| ctrlnum | (OCoLC)255809721 (DE-599)DNB98475511X |
| dewey-full | 615.3 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 615 - Pharmacology and therapeutics |
| dewey-raw | 615.3 |
| dewey-search | 615.3 |
| dewey-sort | 3615.3 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| discipline_str_mv | Medizin |
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| spelling | Neugebauer, Ute 1978- Verfasser (DE-588)13305456X aut Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy von Ute Neugebauer 2007 VI, 259 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2007 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016696080&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Neugebauer, Ute 1978- Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |
| subject_GND | (DE-588)4113937-9 |
| title | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |
| title_auth | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |
| title_exact_search | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |
| title_exact_search_txtP | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |
| title_full | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy von Ute Neugebauer |
| title_fullStr | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy von Ute Neugebauer |
| title_full_unstemmed | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy von Ute Neugebauer |
| title_short | Characterization of bacteria, antibiotics of the fluoroquinolone type and their biological targets DNA and gyrase utilizing the unique potential of vibrational spectroscopy |
| title_sort | characterization of bacteria antibiotics of the fluoroquinolone type and their biological targets dna and gyrase utilizing the unique potential of vibrational spectroscopy |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016696080&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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