Pichia protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, N.J.
Humana Press
2007
|
| Edition: | 2. ed. |
| Series: | Methods in molecular biology
389 |
| Subjects: | |
| Online Access: | Table of contents Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | xi, 268 p. ill. |
| ISBN: | 9781588294296 |
Staff View
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| 245 | 1 | 0 | |a Pichia protocols |c edited by James M. Cregg |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2007 | |
| 300 | |a xi, 268 p. |b ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 389 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Pichia pastoris | |
| 650 | 4 | |a Yeast fungi |x Biotechnology | |
| 650 | 4 | |a Genetic vectors | |
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Record in the Search Index
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| adam_text | METHODSINMOLECULARBIOLOGY
Pichia Protocols
SECOND EDITION
Edited by
James M Cregg
Keck Graduate Institute of Applied Life Sciences,
Claremont, CA
HUMANA PRESS ^ £ TOTOWA, NEW JERSEY
Contents
Preface v
Contributors ix
1 Introduction: Distinction Between Pichia pastoris
and Other Expression Systems 1
fames M Cregg
2 Vectors and Strains for Expression 11
Joan Lin-Cereghino and Geoff P Lin-Cereghino
3 DNA-Mediated Transformation 27
James M Cregg
4 Rational Design and Optimization of Fed-Batch
and Continuous Fermentations 43
Wenhui Zhang, Mehmet lhan, and Michael M Meagher
5 Saturation of the Secretory Pathway by Overexpression
of a Hookworm (Necator americanus) Protein (Na-ASP1) 65
Mehmet Inan, Sarah A Fanders, Wenhui Zhang, Peter J Hotez,
Bin Zhan, and Michael M Meagher
6 Purification of the N- and C-Terminal Subdomains of Recombinant
Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C 77
Jicai Huang, Rick Barent, Mehmet Inan, Mark Gouthro,
Virginia P Roxas, Leonard A Smith, and Michael M Meagher
7 Rapid Screening of Chromatography Resins
for the Purification of Proteins 99
Sandra E Rios, Erin M Giaccone, and Tillman U Gerngross
8 Characterization of O-Linked Saccharides on Glycoproteins 107
Roger K Bretthauer -
9 Modification of the N-Glycosylation Pathway
to Produce Homogeneous Human-Like Glycans
Using GlycoSwitch Plasmids 119
Wouter Vervecken, Nico Callewaert, Vladimir Kaigorodov,
Steven Geysens, and Roland Contreras
10 N-Linked Glycan Characterization of Heterologous Proteins 139
Huijan Li, Robert G Miele, Teresa I Mitchell,
and Tillman U Gerngross
11 Heavy Labeling of Recombinant Proteins 151
Eric Rodriguez
vii
viii Contents
12 Selenomethionine Labeling of Recombinant Proteins 165
Anna M Larsson and T Alwyn Jones
13 Selective Isotopic Labeling of Recombinant Proteins Using
Amino Acid Auxotroph Strains 175
James W Whittaker
14 Classical Genetics 189
llya Tolstorukov and James M Cregg
15 Identification of Pexophagy Genes by Restriction
Enzyme-Mediated Integration 203
Laura A Schroder, Benjamin S Glick, and William A Dunn, Jr
16 Characterization of Protein-Protein Interactions:
Application to the Understanding of Peroxisome Biogenesis 219
Sebastien Leon, Ivet Suriapranata, Mingda Yan,
Naganand Rayapuram, Amar Patel, and Suresh Subramani
17 Localization of Proteins and Organelles Using Fluorescence
Microscopy 239
Jean-Claude Farre, Kanae Shirahama-Noda, Lan Zhang,
Keith Booher, and Suresh Subramani
18 Fluorescence Microscopy and Thin-Section Electron Microscopy 251
Benjamin S Glick
Index 261
O
|
| adam_txt |
METHODSINMOLECULARBIOLOGY
Pichia Protocols
SECOND EDITION
Edited by
James M Cregg
Keck Graduate Institute of Applied Life Sciences,
Claremont, CA
HUMANA PRESS ^ £ TOTOWA, NEW JERSEY
Contents
Preface v
Contributors ix
1 Introduction: Distinction Between Pichia pastoris
and Other Expression Systems 1
fames M Cregg
2 Vectors and Strains for Expression 11
Joan Lin-Cereghino and Geoff P Lin-Cereghino
3 DNA-Mediated Transformation 27
James M Cregg
4 Rational Design and Optimization of Fed-Batch
and Continuous Fermentations 43
Wenhui Zhang, Mehmet lhan, and Michael M Meagher
5 Saturation of the Secretory Pathway by Overexpression
of a Hookworm (Necator americanus) Protein (Na-ASP1) 65
Mehmet Inan, Sarah A Fanders, Wenhui Zhang, Peter J Hotez,
Bin Zhan, and Michael M Meagher
6 Purification of the N- and C-Terminal Subdomains of Recombinant
Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C 77
Jicai Huang, Rick Barent, Mehmet Inan, Mark Gouthro,
Virginia P Roxas, Leonard A Smith, and Michael M Meagher
7 Rapid Screening of Chromatography Resins
for the Purification of Proteins 99
Sandra E Rios, Erin M Giaccone, and Tillman U Gerngross
8 Characterization of O-Linked Saccharides on Glycoproteins 107
Roger K Bretthauer - '
9 Modification of the N-Glycosylation Pathway
to Produce Homogeneous Human-Like Glycans
Using GlycoSwitch Plasmids 119
Wouter Vervecken, Nico Callewaert, Vladimir Kaigorodov,
Steven Geysens, and Roland Contreras
10 N-Linked Glycan Characterization of Heterologous Proteins 139
Huijan Li, Robert G Miele, Teresa I Mitchell,
and Tillman U Gerngross
11 Heavy Labeling of Recombinant Proteins 151
Eric Rodriguez
vii
viii Contents
12 Selenomethionine Labeling of Recombinant Proteins 165
Anna M Larsson and T Alwyn Jones
13 Selective Isotopic Labeling of Recombinant Proteins Using
Amino Acid Auxotroph Strains 175
James W Whittaker
14 Classical Genetics 189
llya Tolstorukov and James M Cregg
15 Identification of Pexophagy Genes by Restriction
Enzyme-Mediated Integration 203
Laura A Schroder, Benjamin S Glick, and William A Dunn, Jr
16 Characterization of Protein-Protein Interactions:
Application to the Understanding of Peroxisome Biogenesis 219
Sebastien Leon, Ivet Suriapranata, Mingda Yan,
Naganand Rayapuram, Amar Patel, and Suresh Subramani
17 Localization of Proteins and Organelles Using Fluorescence
Microscopy 239
Jean-Claude Farre, Kanae Shirahama-Noda, Lan Zhang,
Keith Booher, and Suresh Subramani
18 Fluorescence Microscopy and Thin-Section Electron Microscopy 251
Benjamin S Glick
Index 261
O |
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| edition | 2. ed. |
| format | Book |
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| index_date | 2024-07-02T21:46:37Z |
| indexdate | 2024-07-09T21:20:24Z |
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| isbn | 9781588294296 |
| language | English |
| lccn | 2007932487 |
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| physical | xi, 268 p. ill. |
| publishDate | 2007 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Pichia protocols edited by James M. Cregg 2. ed. Totowa, N.J. Humana Press 2007 xi, 268 p. ill. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 389 Includes bibliographical references and index Pichia pastoris Yeast fungi Biotechnology Genetic vectors Pichia pastoris (DE-588)4407211-9 gnd rswk-swf Pichia (DE-588)4273120-3 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Vektor Genetik (DE-588)4279246-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pichia pastoris (DE-588)4407211-9 s Vektor Genetik (DE-588)4279246-0 s DE-604 Pichia (DE-588)4273120-3 s Biotechnologie (DE-588)4069491-4 s Cregg, James M. Sonstige oth Erscheint auch als Online-Ausgabe 978-1-59745-456-8 Methods in molecular biology 389 (DE-604)BV035362695 389 http://www.loc.gov/catdir/toc/fy0802/2007932487.html Table of contents HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016690981&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pichia protocols Methods in molecular biology Pichia pastoris Yeast fungi Biotechnology Genetic vectors Pichia pastoris (DE-588)4407211-9 gnd Pichia (DE-588)4273120-3 gnd Biotechnologie (DE-588)4069491-4 gnd Vektor Genetik (DE-588)4279246-0 gnd |
| subject_GND | (DE-588)4407211-9 (DE-588)4273120-3 (DE-588)4069491-4 (DE-588)4279246-0 (DE-588)4143413-4 |
| title | Pichia protocols |
| title_auth | Pichia protocols |
| title_exact_search | Pichia protocols |
| title_exact_search_txtP | Pichia protocols |
| title_full | Pichia protocols edited by James M. Cregg |
| title_fullStr | Pichia protocols edited by James M. Cregg |
| title_full_unstemmed | Pichia protocols edited by James M. Cregg |
| title_short | Pichia protocols |
| title_sort | pichia protocols |
| topic | Pichia pastoris Yeast fungi Biotechnology Genetic vectors Pichia pastoris (DE-588)4407211-9 gnd Pichia (DE-588)4273120-3 gnd Biotechnologie (DE-588)4069491-4 gnd Vektor Genetik (DE-588)4279246-0 gnd |
| topic_facet | Pichia pastoris Yeast fungi Biotechnology Genetic vectors Pichia Biotechnologie Vektor Genetik Aufsatzsammlung |
| url | http://www.loc.gov/catdir/toc/fy0802/2007932487.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016690981&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT creggjamesm pichiaprotocols |