Holdings: Plant biotechnology and genetics

Plant biotechnology and genetics: principles, techniques, and applications

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Bibliographic Details
Format:	Book
Language:	English
Published:	Hoboken, NJ Wiley 2008
Subjects:	Plant biotechnology Plant genetics Transgenic plants Biotechnologie Molekulargenetik Pflanzen Aufsatzsammlung
Online Access:	Publisher description Table of contents only Contributor biographical information Inhaltsverzeichnis
Physical Description:	XXIV, 374 S. Ill., graph. Darst. 1 CD-ROM (12 cm)
ISBN:	9780470043813

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MARC


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adam_text	PREFACE XVII FOREWORD TO PLANT BIOTECHNOLOGY AND GENETICS XIX XXILI 1. PLANT AGRICULTURE: THE IMPACT OF BIOTECHNOLOGY 1 GRAHAM BROOKES 1.0 CHAPTER SUMMARY AND OBJECTIVES 1 1.0.1 SUMMARY 1 1.0.2 DISCUSSION QUESTIONS 1 1.1 INTRODUCTION 1 1.2 BIOTECHNOLOGY CROPS PLANTINGS 2 1.3 WHY FARMERS USE BIOTECH CROPS 4 1.3.1 HERBICIDE-TOLERANT CROPS 7 1.3.2 INSECT-RESISTANT CROPS 7 1.3.3 CONCLUSION 8 1.4 HOW THE ADOPTION OF PLANT BIOTECHNOLOGY HAS IMPACTED THE ENVIRONMENT 8 1.4.1 ENVIRONMENTAL IMPACTS FROM CHANGES IN INSECTICIDE AND HERBICIDE USE 9 1.4.2 IMPACT ON GREENHOUSE GAS (GHG) EMISSIONS 12 1.5 CONCLUSIONS 14 REFERENCES 19 2. MEMDELIAN GENETICS AND PLANT REPRODUCTION 21. MATTHEW D. HALFHILL AND SUZANNE I. WARWICK 2.0 CHAPTER SUMMARY AND OBJECTIVES 21 2.0.1 SUMMARY 21 2.0.2 DISCUSSION QUESTIONS 21 2.1 GENETICS OVERVIEW 22 2.2 MENDELIAN GENETICS 25 2.2.1 LAW OF SEGREGATION 28 2.2.2 LAW OF INDEPENDENT ASSORTMENT 28 2.3 MITOSIS AND MEIOSIS 30 2.3.1 MITOSIS 31 2.3.2 MEIOSIS 32 2.3.3 RECOMBINATION 32 2.3.4 CYTOGENETIC ANALYSIS 33 VI! 181 CONTENTS 2.4 PLANT REPRODUCTIVE BIOLOGY 34 2.4.1 HISTOR Y OF RESEARCH 34 2.4.2 MATING SYSTEMS 35 2.4.2.1 SEXUAL REPRODUCTION 35 2.4.2.2 ASEXUAL REPRODUCTION 38 2.4.2.3 MATING SYSTEMS SUMMARY 38 2.4.3 HYBRIDIZATION AND POLYPLOIDY 39 2.5 CONCLUSION 41 REFERENCES 45 3. PLANT BREEDING 47 NICHOLAS A. TINKER 3.0 CHAPTER SUMMARY AND OBJECTIVES 47 3.0.1 SUMMARY 47 3.0.2 DISCUSSION QUESTIONS 47 3.1 INTRODUCTION 48 3.2 CENTRAL CONCEPTS IN PLANT BREEDING 49 3.2.1 SIMPLE VERSUS COMPLEX INHERITANCE 49 3.2.2 PHENOTYPE VERSUS GENOTYPE 51 3.2.3 MATING SYSTEMS, VARIETIES, LANDRACES, AND PURE LINES 52 3.2.4 OTHER TOPICS IN POPULATION AND QUANTITATIVE GENETICS 55 3.2.5 THE VALUE OF A PLANT VARIETY DEPENDS ON MANY TRAITS 56 3.2.6 VARIETIES MUST BE ADAPTED TO ENVIRONMENTS 56 3.2.7 PLANT BREEDING IS A NUMBERS GAME 57 3.2.8 PLANT BREEDING IS AN ITERATIVE AND COLLABORATIVE PROCESS 57 3.2.9 DIVERSITY, ADAPTATION, AND IDEOTYPES 58 3.2.10 OTHER CONSIDERATIONS 61 3.3 OBJECTIVES FOR PLANT BREEDING 62 3.4 METHODS OF PLANT BREEDING 63 3.4.1 METHODS OF HYBRIDIZATION 63 3.4.1.1 SELF-POLLINATED SPECIES 64 3.4.1.2 OUTCROSSING SPECIES 69 3.4.1.3 SYNTHETIC VARIETIES 72 3.4.1.4 HYBRID VARIETIES 72 3.4.2 CLONALLY PROPAGATED SPECIES 74 3.5 BREEDING ENHANCEMENTS 74 3.5.1 DOUBLED HAPLOIDY 74 3.5.2 MARKER-ASSISTED SELECTION 75 3.5.3 MUTATION BREEDING 76 3.5.4 APOMIXIS 77 3.6 CONCLUSIONS 77 REFERENCES 82 4. PLANT DEVELOPMENT AND PHYSIOLOGY 83 GLENDA E. GILLASPY 4.0 CHAPTER SUMMARY AND OBJECTIVES 83 4.0.1 SUMMARY 83 4.0.2 DISCUSSION QUESTIONS 83 4.1 PLANT ANATOMY AND MORPHOLOGY 84 CONTENTS IX 4.2 EMBRYOGENESIS AND SEED GERMINATION 85 4.2.1 GAMETOGENESIS 85 4.2.2 FERTILIZATION 88 4.2.3 FRUIT DEVELOPMENT 88 4.2.4 EMBRYOGENESIS 89 4.2.5 SEED GERMINATION 91 4.2.6 PHOTOMORPHOGENESIS 91 4.3 MERISTEMS 92 4.3.1 SHOOT APICAL MERISTEM 92 4.3.2 ROOT APICAL MERISTEM AND ROOT DEVELOPMENT 94 4.4 LEAF DEVELOPMENT 96 4.4.1 LEAF STRUCTURE 96 4.4.2 LEAF DEVELOPMENT PATTERNS 97 4.5 FLOWER DEVELOPMENT 98 4.5.1 FLORAL EVOCATION 98 4.5.2 FLORAL ORGAN IDENTITY AND THE ABC MODEL 99 4.6 HORMONE PHYSIOLOGY AND SIGNAL TRANSDUCTION 101 4.6.1 SEVEN PLANT HORMONES AND THEIR ACTIONS 101 4.6.2 PLANT HORMONE SIGNAL TRANSDUCTION 103 4.6.2.1 AUXIN AND GA SIGNALING 104 4.6.2.2 CYTOKININ AND ETHYLENE SIGNALING 105 4.6.2.3 BRASSINOSTEROID SIGNAL TRANSDUCTION 105 4.7 CONCLUSIONS 106 REFERENCES 110 5. TISSUE CULTURE: THE MANIPULATION OF PLANT DEVELOPMENT 113 VINITHA CARDOZA 113 113 313 113 114 115 115 116 118 118 118 119 120 120 122 122 122 123 123 124 124 124 5.0 5.1 5.2 5.3 5.4 5.5 5.6 CHAPTER SUMMARY AND OBJECTIVES 5.0.1 5.0.2 SUMMARY DISCUSSION QUESTIONS INTRODUCTION HISTORY MEDIA 5.3.1 5.3.2 STERILE 5.4.1 5.4.2 AND CULTURE CONDITIONS BASAL MEDIA GROWTH REGULATORS TECHNIQUE CLEAN EQUIPMENT SURFACE STERILIZATION OF EXPLANTS CULTURE CONDITIONS AND VESSELS CULTURE TYPES AND THEIR USES 5.6.1 5.6.2 5.6.3 5.6.4 5.6.5 5.6.6 CALLUS CULTURE 5.6.1.1 SOMACLONAL VARIATION CELL SUSPENSION CULTURE 5.6.2.1 PRODUCTION OF SECONDARY METABOLITES AND RECOMBINANT PROTEINS USING CELL CULTURE ANTHER/MICROSPORE CULTURE PROTOPLAST CULTURE 5.6.4.1 SOMATIC HYBRIDIZATION EMBRYO CULTURE MERISTEM CULTURE X CONTENTS 5.7 REGENERATION METHODS OF PLANTS IN CULTURE 125 5.7.1 ORGANOGENESIS 125 5.7.1.1 INDIRECT ORGANOGENESIS 125 5.7.1.2 DIRECT ORGANOGENESIS 125 5.7.2 SOMATIC EMBRYOGENESIS 126 5.7.2.1 SYNTHETIC SEEDS 127 5.8 ROOTING OF SHOOTS 127 5.9 ACCLIMATION 128 5.10 CONCLUSIONS 128 ACKNOWLEDGMENTS 128 REFERENCES 132 6. MOLECULAR GENETICS OF GENE EXPRESSION 135 MARIA GALLO AND ALISON K. FLYNN 6.0 CHAPTER SUMMARY AND OBJECTIVES 135 6.0.1 SUMMARY 135 6.0.2 DISCUSSION QUESTIONS 135 6.1 THE GENE 135 6.1.1 DNA CODING FOR A PROTEIN VIA THE GENE 135 6.1.2 DNA AS A POLYNUCLEOTIDE 136 6.2 DNA PACKAGING INTO EUKARYOTIC CHROMOSOMES 136 6.3 TRANSCRIPTION 140 6.3.1 TRANSCRIPTION OF DNA TO PRODUCE MESSENGER RNA (MRNA) 140 6.3.2 TRANSCRIPTION FACTORS 143 6.3.3 COORDINATED REGULATION OF GENE EXPRESSION 144 6.3.4 CHROMATIN AS AN IMPORTANT REGULATOR OF TRANSCRIPTION 144 6.3.5 REGULATION OF GENE EXPRESSION BY DNA METHYLATION 146 6.3.6 PROCESSING TO PRODUCE MATURE MRNA 146 6.4 TRANSLATION 148 6.4.1 INITIATION OF TRANSLATION 150 6.4.2 TRANSLATION ELONGATION 152 6.4.3 TRANSLATION TERMINATION 152 6.5 PROTEIN POSTRANSLATIONAL MODIFICATION 152 REFERENCES 156 7. RECOMBINANT DNA, VECTOR DESIGN, AND CONSTRUCTION 159 MARK D. CURTIS 7.0 CHAPTER SUMMARY AND OBJECTIVES 159 7.0.1 SUMMARY 159 7.0.2 DISCUSSION QUESTIONS 159 7.1 DNA MODIFICATION 160 7.2 DNA VECTORS 163 7.2.1 DNA VECTORS FOR PLANT TRANSFORMATION 166 7.2.2 COMPONENTS FOR EFFICIENT GENE EXPRESSION IN PLANTS 167 7.3 GREATER DEMANDS LEAD TO INNOVATION 170 7.3.1 SITE-SPECIFIC DNA RECOMBINATION 171 CONTENTS XI 7.3.1.1 GATEWAY CLONING 172 7.3.1.2 CREATOR* CLONING 175 7.3.1.3 UNIVECTOR (ECHO*) CLONING 175 7.4 VECTOR DESIGN 177 7.4.1 VECTORS FOR HIGH-THROUGHPUT FUNCTIONAL ANALYSIS 177 7.4.2 VECTORS FOR RNA INTERFERENCE (RNAI) 179 7.4.3 EXPRESSION VECTORS 179 7.4.4 VECTORS FOR PROMOTER ANALYSIS 180 7.4.5 VECTORS DERIVED FROM PLANT SEQUENCES 181 7.4.6 VECTORS FOR MULTIGENIC TRAITS 183 7.5 TARGETED TRANSGENE INSERTIONS 184 7.6 SAFETY FEATURES IN VECTOR DESIGN 186 7.7 PROSPECTS 188 REFERENCES 190 8. GENES AND TRAITS OF INTEREST FOR TRANSGENIC PLANTS 193 KENNETH L. KORTH 8.0 CHAPTER SUMMARY AND OBJECTIVES 193 8.0.1 SUMMARY 193 8.0.2 DISCUSSION QUESTIONS 193 8.3 INTRODUCTION 194 8.2 IDENTIFYING GENES OF INTEREST VIA GENOMIC STUDIES 194 8.3 TRAITS FOR IMPROVED CROP PRODUCTION 197 8.3.1 HERBICIDE RESISTANCE 197 8.3.2 INSECT RESISTANCE 200 8.3.3 PATHOGEN RESISTANCE 202 8.4 TRAITS FOR IMPROVED PRODUCTS AND FOOD QUALITY 205 8.4.1 NUTRITIONAL IMPROVEMENTS 205 8.4.2 MODIFIED PLANT OILS 207 8.4.3 PHARMACEUTICAL PRODUCTS 208 8.4.4 BIOFUELS 209 8.5 CONCLUSIONS 210 REFERENCES 216 9. MARKER GENES AND PROMOTERS 217 BRIAN MIKI 9.0 CHAPTER SUMMARY AND OBJECTIVES 217 9.0.1 SUMMARY 217 9.0.2 DISCUSSION QUESTIONS 217 9.1 INTRODUCTION 218 9.2 DEFINITION OF MARKER GENES 218 9.2.1 SELECTABLE MARKER GENES: AN INTRODUCTION 218 9.2.2 REPORTER GENES: AN INTRODUCTION 222 9.3 PROMOTERS 224 9.4 SELECTABLE MARKER GENES 227 9.4.1 CONDITIONAL POSITIVE SELECTABLE MARKER GENE SYSTEMS 227 X1S CONTENTS 9.4.1.1 SELECTION ON ANTIBIOTICS 228 9.4.1.2 SELECTION ON HERBICIDES 229 9.4.1.3 SELECTION USING NONTOXIC METABOLIC SUBSTRATES 229 9.4.2 NONCONDITIONAL POSITIVE SELECTION SYSTEMS 230 9.4.3 CONDITIONAL NEGATIVE SELECTION SYSTEMS 230 9.4.4 NONCONDITIONAL NEGATIVE SELECTION SYSTEMS 230 9.5 NONSELECTABLE MARKER GENES OR REPORTER GENES 231 9.5.1 /3-GLUCURONIDASE 231 9.5.2 LUC IF ERASE 232 9.5.3 GREEN FLUORESCENT PROTEIN 232 9.6 MARKER-FREE STRATEGIES 233 9.7 CONCLUSIONS 237 REFERENCES 242 10. TRANSGENIC PLANT PRODUCTION 245 JOHN FINER AND TANIYA DHILLON 10.0 CHAPTER SUMMARY AND OBJECTIVES 245 10.0.1 SUMMARY 245 10.0.2 DISCUSSION QUESTIONS 245 10.1 OVERVIEW 246 10.2 BASIC COMPONENTS FOR SUCCESSFUL GENE TRANSFER TO PLANT CELLS 246 10.2.1 VISUALIZING THE GENERAL TRANSFORMATION PROCESS 246 10.2.2 DNA DELIVERY 247 10.2.3 TARGET TISSUE STATUS 248 10.2.4 SELECTION AND REGENERATION 248 10.3 AGROBACTERIUM 249 10.3.1 HISTORY OF OUR KNOWLEDGE OF AGROBACTERIUM 249 10.3.2 USE OF THE T-DNA TRANSFER PROCESS FOR TRANSFORMATION 251 10.3.3 OPTIMIZING DELIVERY AND BROADENING THE RANGE OF TARGETS 253 10.3.4 AGROINFILTRATION 254 10.3.5 ARABIDOPSIS FLORAL DIP 254 10.4 PARTICLE BOMBARDMENT 255 10.4.1 HISTORY OF PARTICLE BOMBARDMENT 255 10.4.2 THE FATE OF INTRODUCED DNA 257 10.4.3 THE POWER AND PROBLEMS OF DIRECT DNA INTRODUCTION 259 10.4.4 IMPROVEMENTS IN TRANSGENE EXPRESSION 259 10.5 OTHER METHODS 260 10.5.1 THE NEED FOR ADDITIONAL TECHNOLOGIES 260 10.5.2 PROTOPLASTS 261 10.5.3 WHOLE-TISSUE ELECTROPORATION 262 10.5.4 SILICON CARBIDE WHISKERS 262 10.5.5 VIRAL VECTORS 263 10.5.6 LASER MICROPUNCTURE 263 10.5.7 NANOFIBER ARRAYS 263 10.6 THE RUSH TO PUBLISH 265 10.6.1 CONTROVERSIAL REPORTS OF PLANT TRANSFORMATION 265 10.6.1.1 DNA UPTAKE IN POLLEN 265 10.6.1.2 AGROBACTERIUM-MEDIAXED TRANSFORMATION OF MAIZE SEEDLINGS 265 CONTENTS XLI! 10.6.1.3 POLLEN TUBE PATHWAY 266 10.6.1.4 RYE FLORAL TILLER INJECTION 266 10.6.1.5 ELECTROTRANSFORMATION OF GERMINATING POLLEN GRAIN 267 10.6.1.6 MEDICAGO TRANSFORMATION VIA SEEDLING INFILTRATION 267 10.6.2 CRITERIA TO CONSIDER: WHETHER MY PLANT IS TRANSGENIC 268 10.6.2.1 RESISTANCE GENES 268 10.6.2.2 MARKER GENES 268 10.6.2.3 TRANSGENEDNA 269 10.7 A LOOK TO THE FUTURE 269 REFERENCES 272 11. TRANSGENIC PLANT ANALYSIS 275 JANICE ZALE 11.0 CHAPTER SUMMARY AND OBJECTIVES 275 11.0.1 SUMMARY 275 11.0.2 DISCUSSION QUESTIONS 275 11.1 INTRODUCTION 276 11.2 DIRECTIONALLY NAMED ANALYSES: AS THE COMPASS TURNS 276 11.3 INITIAL SCREENS: PUTATIVE TRANSGENIC PLANTS 277 11.3.1 SCREENS ON SELECTION MEDIA 277 11.3.2 POLYMERASE CHAIN REACTION 278 11.3.3 ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISAS) 279 11.4 DEFINITIVE MOLECULAR CHARACTERIZATION 280 11.4.1 INTACT TRANSGENE INTEGRATION 280 1 1.4.2 DETERMINING THE PRESENCE OF INTACT TRANSGENES OR CONSTRUCTS 284 11.4.3 TRANSGENE EXPRESSION: TRANSCRIPTION 284 11.4.3.1 NORTHERN BLOT ANALYSIS 284 11.4.3.2 QUANTITATIVE REAL-TIME REVERSE TRANSCRIPTASE (RT)-PCR 286 11.4.4 TRANSGENE EXPRESSION: TRANSLATION: WESTERN BLOT ANALYSES 286 11.5 DIGITAL IMAGING 287 11.6 PHENOTYPIC ANALYSIS 287 11.7 CONCLUSIONS 288 REFERENCES 288 12. REGULATIONS AND BIOSAFETY 291 ALAN MCHUGHEN 12.0 CHAPTER SUMMARY AND OBJECTIVES 291 12.0.1 SUMMARY 291 12.0.2 DISCUSSION QUESTIONS 291 12.1 INTRODUCTION 291 12.2 HISTORY OF GENETIC ENGINEERING AND ITS REGULATION 293 12.3 REGULATION OF GE 296 12.3.1 UNITED STATES 296 12.3.1.1 USDA 297 12.3.1.2 FDA 297 12.3.1.3 EPA 298 12.3.2 EU 299 12.3.3 CANADA 300
adam_txt	PREFACE XVII FOREWORD TO PLANT BIOTECHNOLOGY AND GENETICS XIX XXILI 1. PLANT AGRICULTURE: THE IMPACT OF BIOTECHNOLOGY 1 GRAHAM BROOKES 1.0 CHAPTER SUMMARY AND OBJECTIVES 1 1.0.1 SUMMARY 1 1.0.2 DISCUSSION QUESTIONS 1 1.1 INTRODUCTION 1 1.2 BIOTECHNOLOGY CROPS PLANTINGS 2 1.3 WHY FARMERS USE BIOTECH CROPS 4 1.3.1 HERBICIDE-TOLERANT CROPS 7 1.3.2 INSECT-RESISTANT CROPS 7 1.3.3 CONCLUSION 8 1.4 HOW THE ADOPTION OF PLANT BIOTECHNOLOGY HAS IMPACTED THE ENVIRONMENT 8 1.4.1 ENVIRONMENTAL IMPACTS FROM CHANGES IN INSECTICIDE AND HERBICIDE USE 9 1.4.2 IMPACT ON GREENHOUSE GAS (GHG) EMISSIONS 12 1.5 CONCLUSIONS 14 REFERENCES 19 2. MEMDELIAN GENETICS AND PLANT REPRODUCTION 21. MATTHEW D. HALFHILL AND SUZANNE I. WARWICK 2.0 CHAPTER SUMMARY AND OBJECTIVES 21 2.0.1 SUMMARY 21 2.0.2 DISCUSSION QUESTIONS 21 2.1 GENETICS OVERVIEW 22 2.2 MENDELIAN GENETICS 25 2.2.1 LAW OF SEGREGATION 28 2.2.2 LAW OF INDEPENDENT ASSORTMENT 28 2.3 MITOSIS AND MEIOSIS 30 2.3.1 MITOSIS 31 2.3.2 MEIOSIS 32 2.3.3 RECOMBINATION 32 2.3.4 CYTOGENETIC ANALYSIS 33 VI! 181 CONTENTS 2.4 PLANT REPRODUCTIVE BIOLOGY 34 2.4.1 HISTOR Y OF RESEARCH 34 2.4.2 MATING SYSTEMS 35 2.4.2.1 SEXUAL REPRODUCTION 35 2.4.2.2 ASEXUAL REPRODUCTION 38 2.4.2.3 MATING SYSTEMS SUMMARY 38 2.4.3 HYBRIDIZATION AND POLYPLOIDY 39 2.5 CONCLUSION 41 REFERENCES 45 3. PLANT BREEDING 47 NICHOLAS A. TINKER 3.0 CHAPTER SUMMARY AND OBJECTIVES 47 3.0.1 SUMMARY 47 3.0.2 DISCUSSION QUESTIONS 47 3.1 INTRODUCTION 48 3.2 CENTRAL CONCEPTS IN PLANT BREEDING 49 3.2.1 SIMPLE VERSUS COMPLEX INHERITANCE 49 3.2.2 PHENOTYPE VERSUS GENOTYPE 51 3.2.3 MATING SYSTEMS, VARIETIES, LANDRACES, AND PURE LINES 52 3.2.4 OTHER TOPICS IN POPULATION AND QUANTITATIVE GENETICS 55 3.2.5 THE VALUE OF A PLANT VARIETY DEPENDS ON MANY TRAITS 56 3.2.6 VARIETIES MUST BE ADAPTED TO ENVIRONMENTS 56 3.2.7 PLANT BREEDING IS A NUMBERS GAME 57 3.2.8 PLANT BREEDING IS AN ITERATIVE AND COLLABORATIVE PROCESS 57 3.2.9 DIVERSITY, ADAPTATION, AND IDEOTYPES 58 3.2.10 OTHER CONSIDERATIONS 61 3.3 OBJECTIVES FOR PLANT BREEDING 62 3.4 METHODS OF PLANT BREEDING 63 3.4.1 METHODS OF HYBRIDIZATION 63 3.4.1.1 SELF-POLLINATED SPECIES 64 3.4.1.2 OUTCROSSING SPECIES 69 3.4.1.3 SYNTHETIC VARIETIES 72 3.4.1.4 HYBRID VARIETIES 72 3.4.2 CLONALLY PROPAGATED SPECIES 74 3.5 BREEDING ENHANCEMENTS 74 3.5.1 DOUBLED HAPLOIDY 74 3.5.2 MARKER-ASSISTED SELECTION 75 3.5.3 MUTATION BREEDING 76 3.5.4 APOMIXIS 77 3.6 CONCLUSIONS 77 REFERENCES 82 4. PLANT DEVELOPMENT AND PHYSIOLOGY 83 GLENDA E. GILLASPY 4.0 CHAPTER SUMMARY AND OBJECTIVES 83 4.0.1 SUMMARY 83 4.0.2 DISCUSSION QUESTIONS 83 4.1 PLANT ANATOMY AND MORPHOLOGY 84 CONTENTS IX 4.2 EMBRYOGENESIS AND SEED GERMINATION 85 4.2.1 GAMETOGENESIS 85 4.2.2 FERTILIZATION 88 4.2.3 FRUIT DEVELOPMENT 88 4.2.4 EMBRYOGENESIS 89 4.2.5 SEED GERMINATION 91 4.2.6 PHOTOMORPHOGENESIS 91 4.3 MERISTEMS 92 4.3.1 SHOOT APICAL MERISTEM 92 4.3.2 ROOT APICAL MERISTEM AND ROOT DEVELOPMENT 94 4.4 LEAF DEVELOPMENT 96 4.4.1 LEAF STRUCTURE 96 4.4.2 LEAF DEVELOPMENT PATTERNS 97 4.5 FLOWER DEVELOPMENT 98 4.5.1 FLORAL EVOCATION 98 4.5.2 FLORAL ORGAN IDENTITY AND THE ABC MODEL 99 4.6 HORMONE PHYSIOLOGY AND SIGNAL TRANSDUCTION 101 4.6.1 SEVEN PLANT HORMONES AND THEIR ACTIONS 101 4.6.2 PLANT HORMONE SIGNAL TRANSDUCTION 103 4.6.2.1 AUXIN AND GA SIGNALING 104 4.6.2.2 CYTOKININ AND ETHYLENE SIGNALING 105 4.6.2.3 BRASSINOSTEROID SIGNAL TRANSDUCTION 105 4.7 CONCLUSIONS 106 REFERENCES 110 5. TISSUE CULTURE: THE MANIPULATION OF PLANT DEVELOPMENT 113 VINITHA CARDOZA 113 113 313 113 114 115 115 116 118 118 118 119 120 120 122 122 122 123 123 124 124 124 5.0 5.1 5.2 5.3 5.4 5.5 5.6 CHAPTER SUMMARY AND OBJECTIVES 5.0.1 5.0.2 SUMMARY DISCUSSION QUESTIONS INTRODUCTION HISTORY MEDIA 5.3.1 5.3.2 STERILE 5.4.1 5.4.2 AND CULTURE CONDITIONS BASAL MEDIA GROWTH REGULATORS TECHNIQUE CLEAN EQUIPMENT SURFACE STERILIZATION OF EXPLANTS CULTURE CONDITIONS AND VESSELS CULTURE TYPES AND THEIR USES 5.6.1 5.6.2 5.6.3 5.6.4 5.6.5 5.6.6 CALLUS CULTURE 5.6.1.1 SOMACLONAL VARIATION CELL SUSPENSION CULTURE 5.6.2.1 PRODUCTION OF SECONDARY METABOLITES AND RECOMBINANT PROTEINS USING CELL CULTURE ANTHER/MICROSPORE CULTURE PROTOPLAST CULTURE 5.6.4.1 SOMATIC HYBRIDIZATION EMBRYO CULTURE MERISTEM CULTURE X CONTENTS 5.7 REGENERATION METHODS OF PLANTS IN CULTURE 125 5.7.1 ORGANOGENESIS 125 5.7.1.1 INDIRECT ORGANOGENESIS 125 5.7.1.2 DIRECT ORGANOGENESIS 125 5.7.2 SOMATIC EMBRYOGENESIS 126 5.7.2.1 SYNTHETIC SEEDS 127 5.8 ROOTING OF SHOOTS 127 5.9 ACCLIMATION 128 5.10 CONCLUSIONS 128 ACKNOWLEDGMENTS 128 REFERENCES 132 6. MOLECULAR GENETICS OF GENE EXPRESSION 135 MARIA GALLO AND ALISON K. FLYNN 6.0 CHAPTER SUMMARY AND OBJECTIVES 135 6.0.1 SUMMARY 135 6.0.2 DISCUSSION QUESTIONS 135 6.1 THE GENE 135 6.1.1 DNA CODING FOR A PROTEIN VIA THE GENE 135 6.1.2 DNA AS A POLYNUCLEOTIDE 136 6.2 DNA PACKAGING INTO EUKARYOTIC CHROMOSOMES 136 6.3 TRANSCRIPTION 140 6.3.1 TRANSCRIPTION OF DNA TO PRODUCE MESSENGER RNA (MRNA) 140 6.3.2 TRANSCRIPTION FACTORS 143 6.3.3 COORDINATED REGULATION OF GENE EXPRESSION 144 6.3.4 CHROMATIN AS AN IMPORTANT REGULATOR OF TRANSCRIPTION 144 6.3.5 REGULATION OF GENE EXPRESSION BY DNA METHYLATION 146 6.3.6 PROCESSING TO PRODUCE MATURE MRNA 146 6.4 TRANSLATION 148 6.4.1 INITIATION OF TRANSLATION 150 6.4.2 TRANSLATION ELONGATION 152 6.4.3 TRANSLATION TERMINATION 152 6.5 PROTEIN POSTRANSLATIONAL MODIFICATION 152 REFERENCES 156 7. RECOMBINANT DNA, VECTOR DESIGN, AND CONSTRUCTION 159 MARK D. CURTIS 7.0 CHAPTER SUMMARY AND OBJECTIVES 159 7.0.1 SUMMARY 159 7.0.2 DISCUSSION QUESTIONS 159 7.1 DNA MODIFICATION 160 7.2 DNA VECTORS 163 7.2.1 DNA VECTORS FOR PLANT TRANSFORMATION 166 7.2.2 COMPONENTS FOR EFFICIENT GENE EXPRESSION IN PLANTS 167 7.3 GREATER DEMANDS LEAD TO INNOVATION 170 7.3.1 SITE-SPECIFIC DNA RECOMBINATION 171 CONTENTS XI 7.3.1.1 GATEWAY CLONING 172 7.3.1.2 CREATOR* CLONING 175 7.3.1.3 UNIVECTOR (ECHO*) CLONING 175 7.4 VECTOR DESIGN 177 7.4.1 VECTORS FOR HIGH-THROUGHPUT FUNCTIONAL ANALYSIS 177 7.4.2 VECTORS FOR RNA INTERFERENCE (RNAI) 179 7.4.3 EXPRESSION VECTORS 179 7.4.4 VECTORS FOR PROMOTER ANALYSIS 180 7.4.5 VECTORS DERIVED FROM PLANT SEQUENCES 181 7.4.6 VECTORS FOR MULTIGENIC TRAITS 183 7.5 TARGETED TRANSGENE INSERTIONS 184 7.6 SAFETY FEATURES IN VECTOR DESIGN 186 7.7 PROSPECTS 188 REFERENCES 190 8. GENES AND TRAITS OF INTEREST FOR TRANSGENIC PLANTS 193 KENNETH L. KORTH 8.0 CHAPTER SUMMARY AND OBJECTIVES 193 8.0.1 SUMMARY 193 8.0.2 DISCUSSION QUESTIONS 193 8.3 INTRODUCTION 194 8.2 IDENTIFYING GENES OF INTEREST VIA GENOMIC STUDIES 194 8.3 TRAITS FOR IMPROVED CROP PRODUCTION 197 8.3.1 HERBICIDE RESISTANCE 197 8.3.2 INSECT RESISTANCE 200 8.3.3 PATHOGEN RESISTANCE 202 8.4 TRAITS FOR IMPROVED PRODUCTS AND FOOD QUALITY 205 8.4.1 NUTRITIONAL IMPROVEMENTS 205 8.4.2 MODIFIED PLANT OILS 207 8.4.3 PHARMACEUTICAL PRODUCTS 208 8.4.4 BIOFUELS 209 8.5 CONCLUSIONS 210 REFERENCES 216 9. MARKER GENES AND PROMOTERS 217 BRIAN MIKI 9.0 CHAPTER SUMMARY AND OBJECTIVES 217 9.0.1 SUMMARY 217 9.0.2 DISCUSSION QUESTIONS 217 9.1 INTRODUCTION 218 9.2 DEFINITION OF MARKER GENES 218 9.2.1 SELECTABLE MARKER GENES: AN INTRODUCTION 218 9.2.2 REPORTER GENES: AN INTRODUCTION 222 9.3 PROMOTERS 224 9.4 SELECTABLE MARKER GENES 227 9.4.1 CONDITIONAL POSITIVE SELECTABLE MARKER GENE SYSTEMS 227 X1S CONTENTS 9.4.1.1 SELECTION ON ANTIBIOTICS 228 9.4.1.2 SELECTION ON HERBICIDES 229 9.4.1.3 SELECTION USING NONTOXIC METABOLIC SUBSTRATES 229 9.4.2 NONCONDITIONAL POSITIVE SELECTION SYSTEMS 230 9.4.3 CONDITIONAL NEGATIVE SELECTION SYSTEMS 230 9.4.4 NONCONDITIONAL NEGATIVE SELECTION SYSTEMS 230 9.5 NONSELECTABLE MARKER GENES OR REPORTER GENES 231 9.5.1 /3-GLUCURONIDASE 231 9.5.2 LUC IF ERASE 232 9.5.3 GREEN FLUORESCENT PROTEIN 232 9.6 MARKER-FREE STRATEGIES 233 9.7 CONCLUSIONS 237 REFERENCES 242 10. TRANSGENIC PLANT PRODUCTION 245 JOHN FINER AND TANIYA DHILLON 10.0 CHAPTER SUMMARY AND OBJECTIVES 245 10.0.1 SUMMARY 245 10.0.2 DISCUSSION QUESTIONS 245 10.1 OVERVIEW 246 10.2 BASIC COMPONENTS FOR SUCCESSFUL GENE TRANSFER TO PLANT CELLS 246 10.2.1 VISUALIZING THE GENERAL TRANSFORMATION PROCESS 246 10.2.2 DNA DELIVERY 247 10.2.3 TARGET TISSUE STATUS 248 10.2.4 SELECTION AND REGENERATION 248 10.3 AGROBACTERIUM 249 10.3.1 HISTORY OF OUR KNOWLEDGE OF AGROBACTERIUM 249 10.3.2 USE OF THE T-DNA TRANSFER PROCESS FOR TRANSFORMATION 251 10.3.3 OPTIMIZING DELIVERY AND BROADENING THE RANGE OF TARGETS 253 10.3.4 AGROINFILTRATION 254 10.3.5 ARABIDOPSIS FLORAL DIP 254 10.4 PARTICLE BOMBARDMENT 255 10.4.1 HISTORY OF PARTICLE BOMBARDMENT 255 10.4.2 THE FATE OF INTRODUCED DNA 257 10.4.3 THE POWER AND PROBLEMS OF DIRECT DNA INTRODUCTION 259 10.4.4 IMPROVEMENTS IN TRANSGENE EXPRESSION 259 10.5 OTHER METHODS 260 10.5.1 THE NEED FOR ADDITIONAL TECHNOLOGIES 260 10.5.2 PROTOPLASTS 261 10.5.3 WHOLE-TISSUE ELECTROPORATION 262 10.5.4 SILICON CARBIDE WHISKERS 262 10.5.5 VIRAL VECTORS 263 10.5.6 LASER MICROPUNCTURE 263 10.5.7 NANOFIBER ARRAYS 263 10.6 THE RUSH TO PUBLISH 265 10.6.1 CONTROVERSIAL REPORTS OF PLANT TRANSFORMATION 265 10.6.1.1 DNA UPTAKE IN POLLEN 265 10.6.1.2 AGROBACTERIUM-MEDIAXED TRANSFORMATION OF MAIZE SEEDLINGS 265 CONTENTS XLI! 10.6.1.3 POLLEN TUBE PATHWAY 266 10.6.1.4 RYE FLORAL TILLER INJECTION 266 10.6.1.5 ELECTROTRANSFORMATION OF GERMINATING POLLEN GRAIN 267 10.6.1.6 MEDICAGO TRANSFORMATION VIA SEEDLING INFILTRATION 267 10.6.2 CRITERIA TO CONSIDER: WHETHER MY PLANT IS TRANSGENIC 268 10.6.2.1 RESISTANCE GENES 268 10.6.2.2 MARKER GENES 268 10.6.2.3 TRANSGENEDNA 269 10.7 A LOOK TO THE FUTURE 269 REFERENCES 272 11. TRANSGENIC PLANT ANALYSIS 275 JANICE ZALE 11.0 CHAPTER SUMMARY AND OBJECTIVES 275 11.0.1 SUMMARY 275 11.0.2 DISCUSSION QUESTIONS 275 11.1 INTRODUCTION 276 11.2 DIRECTIONALLY NAMED ANALYSES: AS THE COMPASS TURNS 276 11.3 INITIAL SCREENS: PUTATIVE TRANSGENIC PLANTS 277 11.3.1 SCREENS ON SELECTION MEDIA 277 11.3.2 POLYMERASE CHAIN REACTION 278 11.3.3 ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISAS) 279 11.4 DEFINITIVE MOLECULAR CHARACTERIZATION 280 11.4.1 INTACT TRANSGENE INTEGRATION 280 1 1.4.2 DETERMINING THE PRESENCE OF INTACT TRANSGENES OR CONSTRUCTS 284 11.4.3 TRANSGENE EXPRESSION: TRANSCRIPTION 284 11.4.3.1 NORTHERN BLOT ANALYSIS 284 11.4.3.2 QUANTITATIVE REAL-TIME REVERSE TRANSCRIPTASE (RT)-PCR 286 11.4.4 TRANSGENE EXPRESSION: TRANSLATION: WESTERN BLOT ANALYSES 286 11.5 DIGITAL IMAGING 287 11.6 PHENOTYPIC ANALYSIS 287 11.7 CONCLUSIONS 288 REFERENCES 288 12. REGULATIONS AND BIOSAFETY 291 ALAN MCHUGHEN 12.0 CHAPTER SUMMARY AND OBJECTIVES 291 12.0.1 SUMMARY 291 12.0.2 DISCUSSION QUESTIONS 291 12.1 INTRODUCTION 291 12.2 HISTORY OF GENETIC ENGINEERING AND ITS REGULATION 293 12.3 REGULATION OF GE 296 12.3.1 UNITED STATES 296 12.3.1.1 USDA 297 12.3.1.2 FDA 297 12.3.1.3 EPA 298 12.3.2 EU 299 12.3.3 CANADA 300
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spelling	Plant biotechnology and genetics principles, techniques, and applications ed. by C. Neal Stewart Hoboken, NJ Wiley 2008 XXIV, 374 S. Ill., graph. Darst. 1 CD-ROM (12 cm) txt rdacontent n rdamedia nc rdacarrier Plant biotechnology Plant genetics Transgenic plants Biotechnologie (DE-588)4069491-4 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Biotechnologie (DE-588)4069491-4 s Molekulargenetik (DE-588)4039987-4 s DE-604 Stewart, C. Neal Sonstige oth http://www.loc.gov/catdir/enhancements/fy0810/2008002719-d.html Publisher description http://www.loc.gov/catdir/enhancements/fy0810/2008002719-t.html Table of contents only http://www.loc.gov/catdir/enhancements/fy0829/2008002719-b.html Contributor biographical information SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016674283&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk
spellingShingle	Plant biotechnology and genetics principles, techniques, and applications Plant biotechnology Plant genetics Transgenic plants Biotechnologie (DE-588)4069491-4 gnd Molekulargenetik (DE-588)4039987-4 gnd Pflanzen (DE-588)4045539-7 gnd
subject_GND	(DE-588)4069491-4 (DE-588)4039987-4 (DE-588)4045539-7 (DE-588)4143413-4
title	Plant biotechnology and genetics principles, techniques, and applications
title_auth	Plant biotechnology and genetics principles, techniques, and applications
title_exact_search	Plant biotechnology and genetics principles, techniques, and applications
title_exact_search_txtP	Plant biotechnology and genetics principles, techniques, and applications
title_full	Plant biotechnology and genetics principles, techniques, and applications ed. by C. Neal Stewart
title_fullStr	Plant biotechnology and genetics principles, techniques, and applications ed. by C. Neal Stewart
title_full_unstemmed	Plant biotechnology and genetics principles, techniques, and applications ed. by C. Neal Stewart
title_short	Plant biotechnology and genetics
title_sort	plant biotechnology and genetics principles techniques and applications
title_sub	principles, techniques, and applications
topic	Plant biotechnology Plant genetics Transgenic plants Biotechnologie (DE-588)4069491-4 gnd Molekulargenetik (DE-588)4039987-4 gnd Pflanzen (DE-588)4045539-7 gnd
topic_facet	Plant biotechnology Plant genetics Transgenic plants Biotechnologie Molekulargenetik Pflanzen Aufsatzsammlung
url	http://www.loc.gov/catdir/enhancements/fy0810/2008002719-d.html http://www.loc.gov/catdir/enhancements/fy0810/2008002719-t.html http://www.loc.gov/catdir/enhancements/fy0829/2008002719-b.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016674283&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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