Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Gießen
VVB Laufersweiler
2008
|
| Series: | Edition scientifique
|
| Subjects: | |
| Online Access: | Abstract Inhaltsverzeichnis |
| Physical Description: | II, 117 S. Ill., graph. Darst. |
| ISBN: | 3835952625 9783835952621 |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV026695786 | ||
| 003 | DE-604 | ||
| 005 | 20110228 | ||
| 007 | t | ||
| 008 | 110326s2008 ad|| m||| 00||| eng d | ||
| 015 | |a 08,N18,0890 |2 dnb | ||
| 015 | |a 08,H10,3484 |2 dnb | ||
| 016 | 7 | |a 988309084 |2 DE-101 | |
| 020 | |a 3835952625 |9 3-8359-5262-5 | ||
| 020 | |a 9783835952621 |9 978-3-8359-5262-1 | ||
| 024 | 7 | |a urn:nbn:de:hebis:26-opus-56525 |2 urn | |
| 035 | |a (OCoLC)248634735 | ||
| 035 | |a (DE-599)DNB988309084 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-188 | ||
| 082 | 0 | |a 636.1089261 |2 22/ger | |
| 084 | |a 630 |2 sdnb | ||
| 100 | 1 | |a Cavalcanti, Márcia Cristina Oliveira |e Verfasser |0 (DE-588)135770823 |4 aut | |
| 245 | 1 | 0 | |a Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion |c submitted by Márcia Cristina Oliveira Cavalcanti |
| 264 | 1 | |a Gießen |b VVB Laufersweiler |c 2008 | |
| 300 | |a II, 117 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Edition scientifique | |
| 502 | |a Zugl.: Gießen, Univ., Diss., 2008 | ||
| 650 | 7 | |a horses |2 cabt | |
| 650 | 7 | |a reproduction |2 cabt | |
| 650 | 7 | |a spermatogenesis |2 cabt | |
| 650 | 7 | |a testes |2 cabt | |
| 650 | 7 | |a genetic expression |2 cabt | |
| 650 | 7 | |a histones |2 cabt | |
| 650 | 7 | |a fertility |2 cabt | |
| 650 | 7 | |a stallions |2 cabt | |
| 650 | 4 | |a Pferd | |
| 650 | 0 | 7 | |a Genexpression |0 (DE-588)4020136-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Histon H1 |0 (DE-588)4313704-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Hengst |0 (DE-588)4024379-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Spermatogenese |0 (DE-588)4135384-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Hengst |0 (DE-588)4024379-5 |D s |
| 689 | 0 | 1 | |a Spermatogenese |0 (DE-588)4135384-5 |D s |
| 689 | 0 | 2 | |a Histon H1 |0 (DE-588)4313704-0 |D s |
| 689 | 0 | 3 | |a Genexpression |0 (DE-588)4020136-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | |u http://library.vetmed.fu-berlin.de/ResourceList/details/167031 |z Abstract | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022238882&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-022238882 | ||
Record in the Search Index
| _version_ | 1804145305521750016 |
|---|---|
| adam_text | IMAGE 1
1 LITERATURE SURVEY 3
1.1 ANATOMY AND HISTOLOGY OF THE TESTIS 3
1.1.1 SPERMATOGENESIS 3
1.1.2 MITOSIS AND MEIOSIS 6
1.1.3 SPERMATOCYTOGENESIS 7
1.1.4 SPERMIOGENESIS 9
1.1.5 SERTOLICELLS 10
1.1.6 HORMONAL REGULATION 10
1.1.7 MAMMALIAN SPERMATOGENIC CYCLE, STAGE AND WAVE 12
1.1.8 SPERMATOGENESIS OF THE STALLION (EQUUS CABALLUS) 12
1.1.9 CRYPTORCHISM IN THE STALLION 17
1.2 HISTORIE - PROTAMINE REPLACEMENT DURING SPERMATOGENESIS 18
1.2.1 THE LINKER HISTONE 19
1.2.2 TRANSCRIPTIONAL REGULATION OF THE HISTONE HIT GENE 20
1.2.3 TRANSCRIPTIONAL ACTIVATION OF THE HU GENE 21
1.2.4 TRANSCRIPTIONAL REPRESSION OF THE HIT GENE 23
1.3 AIMS OF THE STUDY 26
2 MATERIALS AND METHODS 26
2.1 GENERAL HISTOLOGICAL METHODS 27
2.1.1 TISSUE COLLECTION 27
2.1.2 PARAFFIN MATERIAL 27
2.1.3 HISTOLOGY OF THE TESTICULAR SAMPLES 29
2.2 GENERAL MOLECULAR BIOLOGY METHODS 34
2.2.1 RNA ISOLATION WITH TRIZOL REAGENT 34
2.2.2 REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) 36
2.2.3 AGAROSE GEL ELECTROPHORESIS 39
2.2.4 MEASUREMENT OF NUCLEIC ACID SAMPLES 40
2.2.5 REAL-TIME RT-PCR 41
2.2.6 STATISTICAL ANALYSIS 42
2.3 PARTIAL CLONING OF THE EQUINE HIT MRNA 43
2.4 LN-SITU HYBRIDIZATION OF HIT IN EQUINE TESTIS SECTIONS 45
2.4.1 PRODUCTION OF DIGOXIGENIN (DIG)-LABELED CRNA PROBES 45
2.4.2 IN- SITU HYBRIDIZATION 49
1
GESCANNT DURCH
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/988309084
DIGITALISIERT DURCH
IMAGE 2
2.5 ANTIBODY GENERATION AND VERIFICATION 53
2.5.1 HISTONE ISOLATION 54
2.5.2 WESTERN BLOT ANALYSIS 55
2.5.3 IMMUNOHISTOCHEMISTRY 63
2.7 GENERAL CHEMICALS AND REAGENTS 67
2.7.1 ANTIBODIES 68
2.7.2 EQUIPMENT 69
2.7.3 OTHER MATERIALS 69
2.7.8 KITS 70
2.8 ABBREVIATIONS 71
3RESULTS 73
3.1 THE TESTIS SPECIFIC HISTONE (HIT) AND IST EXPRESSION IN THE TESTIS
73
3.1.1 HIT SEQUENCE AND GENBANK ENTRIES 73
3.1.2 SPECIFIC EXPRESSION OF THE HIT MRNA 76
3.1.3 QUANTITATIVE HIT MRNA AND STATISTICAL ANALYSIS 77
3.1.4 CELL-LOCALIZATIONOF HLTCRNAS IN TESTIS SECTIONS 78
3.1.5 HHPROTEIN EXPRESSION AND CELL-PROTEIN-LOCALIZATION 81
3.2 AGE-DEPENDENT EXPRESSION OF THE TESTIS SPECIFIC HISTONE (HIT) 85
3.2.1 AGE-DEPENDENT HIT MRNA EXPRESSION BY RT-PCR 85
3.2.2 QUANTITATIVE HIT MRNA EXPRESSION AND STATISTICAL ANALYSIS 86
3.2.3 AGE-DEPENDENT HIT CRNA EXPRESSION IN FESTES SECTIONS 87
3.2.4 AGE-DEPENDENT HIT PROTEIN EXPRESSION 88
4DISCUSSION 91
4.1 HIT GENE EXPRESSION 91
4.2 AGE-DEPENDENT EXPRESSION OF THE HIT GENE 93
4.3 CONCLUSION 97
5SUMMARY 99
6 ZUSAMMENFASSUNG 100
7REFERENCES 102
8 LIST OF OYVN PUBLICATIONS 115
9 ACKNOWLEDGEMENTS 117
II
|
| any_adam_object | 1 |
| author | Cavalcanti, Márcia Cristina Oliveira |
| author_GND | (DE-588)135770823 |
| author_facet | Cavalcanti, Márcia Cristina Oliveira |
| author_role | aut |
| author_sort | Cavalcanti, Márcia Cristina Oliveira |
| author_variant | m c o c mco mcoc |
| building | Verbundindex |
| bvnumber | BV026695786 |
| ctrlnum | (OCoLC)248634735 (DE-599)DNB988309084 |
| dewey-full | 636.1089261 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.1089261 |
| dewey-search | 636.1089261 |
| dewey-sort | 3636.1089261 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02350nam a2200613 c 4500</leader><controlfield tag="001">BV026695786</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20110228 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">110326s2008 ad|| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">08,N18,0890</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">08,H10,3484</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">988309084</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">3835952625</subfield><subfield code="9">3-8359-5262-5</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783835952621</subfield><subfield code="9">978-3-8359-5262-1</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">urn:nbn:de:hebis:26-opus-56525</subfield><subfield code="2">urn</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)248634735</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB988309084</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-188</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">636.1089261</subfield><subfield code="2">22/ger</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">630</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Cavalcanti, Márcia Cristina Oliveira</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)135770823</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion</subfield><subfield code="c">submitted by Márcia Cristina Oliveira Cavalcanti</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Gießen</subfield><subfield code="b">VVB Laufersweiler</subfield><subfield code="c">2008</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">II, 117 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Edition scientifique</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Zugl.: Gießen, Univ., Diss., 2008</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">horses</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">reproduction</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">spermatogenesis</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">testes</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">genetic expression</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">histones</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">fertility</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">stallions</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Pferd</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genexpression</subfield><subfield code="0">(DE-588)4020136-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Histon H1</subfield><subfield code="0">(DE-588)4313704-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Hengst</subfield><subfield code="0">(DE-588)4024379-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Spermatogenese</subfield><subfield code="0">(DE-588)4135384-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Hengst</subfield><subfield code="0">(DE-588)4024379-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Spermatogenese</subfield><subfield code="0">(DE-588)4135384-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Histon H1</subfield><subfield code="0">(DE-588)4313704-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Genexpression</subfield><subfield code="0">(DE-588)4020136-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://library.vetmed.fu-berlin.de/ResourceList/details/167031</subfield><subfield code="z">Abstract</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022238882&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-022238882</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV026695786 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:17:20Z |
| institution | BVB |
| isbn | 3835952625 9783835952621 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-022238882 |
| oclc_num | 248634735 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | II, 117 S. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | VVB Laufersweiler |
| record_format | marc |
| series2 | Edition scientifique |
| spelling | Cavalcanti, Márcia Cristina Oliveira Verfasser (DE-588)135770823 aut Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion submitted by Márcia Cristina Oliveira Cavalcanti Gießen VVB Laufersweiler 2008 II, 117 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Edition scientifique Zugl.: Gießen, Univ., Diss., 2008 horses cabt reproduction cabt spermatogenesis cabt testes cabt genetic expression cabt histones cabt fertility cabt stallions cabt Pferd Genexpression (DE-588)4020136-3 gnd rswk-swf Histon H1 (DE-588)4313704-0 gnd rswk-swf Hengst (DE-588)4024379-5 gnd rswk-swf Spermatogenese (DE-588)4135384-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Hengst (DE-588)4024379-5 s Spermatogenese (DE-588)4135384-5 s Histon H1 (DE-588)4313704-0 s Genexpression (DE-588)4020136-3 s DE-604 http://library.vetmed.fu-berlin.de/ResourceList/details/167031 Abstract DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022238882&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cavalcanti, Márcia Cristina Oliveira Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion horses cabt reproduction cabt spermatogenesis cabt testes cabt genetic expression cabt histones cabt fertility cabt stallions cabt Pferd Genexpression (DE-588)4020136-3 gnd Histon H1 (DE-588)4313704-0 gnd Hengst (DE-588)4024379-5 gnd Spermatogenese (DE-588)4135384-5 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4313704-0 (DE-588)4024379-5 (DE-588)4135384-5 (DE-588)4113937-9 |
| title | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion |
| title_auth | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion |
| title_exact_search | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion |
| title_full | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion submitted by Márcia Cristina Oliveira Cavalcanti |
| title_fullStr | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion submitted by Márcia Cristina Oliveira Cavalcanti |
| title_full_unstemmed | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion submitted by Márcia Cristina Oliveira Cavalcanti |
| title_short | Gene expression of the testis-specific histone (H1t) in the spermatogenesis of the stallion |
| title_sort | gene expression of the testis specific histone h1t in the spermatogenesis of the stallion |
| topic | horses cabt reproduction cabt spermatogenesis cabt testes cabt genetic expression cabt histones cabt fertility cabt stallions cabt Pferd Genexpression (DE-588)4020136-3 gnd Histon H1 (DE-588)4313704-0 gnd Hengst (DE-588)4024379-5 gnd Spermatogenese (DE-588)4135384-5 gnd |
| topic_facet | horses reproduction spermatogenesis testes genetic expression histones fertility stallions Pferd Genexpression Histon H1 Hengst Spermatogenese Hochschulschrift |
| url | http://library.vetmed.fu-berlin.de/ResourceList/details/167031 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022238882&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT cavalcantimarciacristinaoliveira geneexpressionofthetestisspecifichistoneh1tinthespermatogenesisofthestallion |