Investigation of human mitochondrial DNA abnormality in colon cancer:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Göttingen
Cuvillier
2008
|
| Edition: | 1. Aufl. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 80 S. Ill., graph. Darst. |
| ISBN: | 9783867277594 |
Staff View
MARC
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Record in the Search Index
| _version_ | 1804145282569469952 |
|---|---|
| adam_text | IMAGE 1
TABLEOF CONTENTS
INTRODUCTION
REVIEW OF THE LITERATURE 8-26
2.1 MITOCHONDRIA 2.1.1 STRUCTURE OF MITOCHONDRIA 2.2 THE MITOCHONDRIAL
GENOME 2.2.1 SPECIAL FEATURES OF MTDNA 2.2.2 MITOCHONDRIAL HAPLOGROUPS
2.3 MITOCHONDRIAL DNA REPLICATION 2.4 MUTATIONS IN MTDNA CAUSE SEVERAL
DISEASES IN HUMANS 2.4.1 EXPLANATION OF THRESHOLD EFFECT IN MITOCHONDRIA
2.5 MTDNA ABNORMALITIES 2.5.1 POINT MUTATIONS 2.6 MITOCHONDRIAL
FIINCTION 2.6.1 ATP PRODUCTION BY MITOCHONDRIA 2.6.2 PRODUCTION OF
REACTIVE OXYGEN SPECIES 2.7 CANCER 2.7.1 COLORECTAL CANCER 2.7.2
MITOCHONDRIAL DNA VARIATION IN COLORECTAL CANCER 2.G ROLE OF APOPTOSIS
IN CANCER DEVELOPMENT 2.G.L APOPTOSIS 2.G.2 ROLE OF MITOCHONDRIA IN
APOPTOSIS 2.G.3 ROLE OF BCL-2 PROTEINS 2.9 IMPORTANCE OF THE
MITOCHONDRIAL ABNORMALITY IN TUMORGENESIS 2.10 AIM OF THE STUDY
3 MATERIALS
3.1 PATIENTS 3.1.1 BLOOD SAMPLES 3.1.2 CONTROLS 3.1.3 TISSUE SAMPLES 3.2
ENZYMES 3.3 PRIMERS 3.4 BUFFERS 3.5 GELS
G G 9
11 11 12 13 13 14 15 15 15 18 19 20 20 21 21 22 23 24 26
27-32
27 27 27 27
28 28 30
31
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/990811476
DIGITALISIERT DURCH
IMAGE 2
4 METHODS 33-39
4.1 DNA EXTRACTION 33
4.1.1 DNA EXTRACTION FROM BLOOD SAMPLES 33
4.1.2 DNA EXTRACTION FROM FORMALIN-FIXED, PARAFFIN EMBEDDED SAMPLES 33
4.2 MULTIPLEXPCR 33
4.3 SOUTHERN BLOTTING 34
4.4 PCR AMPLIFICATION AND SEQUENCE ANALYSIS 34
4.4.1 ANALYSIS OF MITOCHONDRIAL HAPLOGROUP 35
4.5 RFLP ANALYSIS 35
4.5.1 INVESTIGATION OF THE MTDNA MERRF MUTATION IN CRC 35 4.5.2
INVESTIGATION OF THE MTDNA MELAS MUTATION IN CRC 36 4.5.3
INVESTIGATION OF THE MTDNA NARP/MILS MUTATION IN CRC 36 4.5.4
INVESTIGATION OF THE MTDNA LHON MUTATION IN CRC 36
4.6 STATISTICAL ANALYSIS 37
4.6.1 HIERARCHICAL CLUSTERING 37
4.7 ANALYSIS OF MTDNA SOMATIC MUTATION 38
4.7.1 TEMPORAL TEMPERATURE GRADIENT GEL ELECTROPHORESIS (TTGE) 38 4.7.2
SINGLE-STRANDED CONFORMATIONAL POLYMORPHISMS (SSCP) 39
5 RESULTS 40-52
5. 1 LARGE SCALE MTDNA DELETION IN HUMAN COLORECTAL CANCER 40
5.2 HAPLOTYPE ANALYSIS: IDENTIFICATION OF A HIGH RISK POPULATION 43 5.3
HIGH RATE OF MUTATION IN MTDNA D LOOP REGION OF CRC PATIENTS 43 5.4
SEARCH FOR MTDNA MUTATIONS RESPONSIBLE FOR MITOCHONDRIAL DISEASE IN CRC
SAMPLES 47
5.5 ANALYSIS OF MITOCHONDRIAL SOMATIC MUTATION IN GENES INVOLVED IN
RESPIRATORY COMPLEX I 47
5.5.1 MITOCHONDRIAL ND1 GENE ANALYSIS 49
6 DISCUSSION 53-56
6.1 LARGE SCALE MITOCHONDRIAL DELETION IN COLORECTAL CANCER 53
6.2 MITOCHONDRIAL HAPLOTYPE ANALYSIS IN COLORECTAL CANCER 54
6.3 SINGLE NUCLEOTIDE POLYMORPHISMS IN THE MTDNA D-LOOP REGION IN HUMAN
CRC 55
6.4 SEARCH FOR MTDNA MUTATIONS IN CRC SAMPLES CAUSING MITOCHONDRIAL
DISEASE 56
6.5 ANALYSIS OF MITOCHONDRIAL SOMATIC MUTATION IN GENES INVOLVED TO
RESPIRATORY COMPLEX I 56
IMAGE 3
7 SIIMMARY
57
7 ZUSAMMENFASSUNG
58-59
8 REFERENCES
60-71
9 APPENDIX
72-79
|
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| dewey-full | 616.994347 |
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| dewey-ones | 616 - Diseases |
| dewey-raw | 616.994347 |
| dewey-search | 616.994347 |
| dewey-sort | 3616.994347 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| indexdate | 2024-07-09T23:16:58Z |
| institution | BVB |
| isbn | 9783867277594 |
| language | English |
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| spelling | Akouchekian, Mansoureh 1968- Verfasser (DE-588)136758835 aut Investigation of human mitochondrial DNA abnormality in colon cancer von Mansoureh Akouchekian 1. Aufl. Göttingen Cuvillier 2008 80 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Hannover, Univ., Diss., 2008 Dickdarmkrebs (DE-588)4012122-7 gnd rswk-swf Mitochondriale DNS (DE-588)4202301-4 gnd rswk-swf Mutation (DE-588)4170883-0 gnd rswk-swf Carcinogenese (DE-588)4069853-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Dickdarmkrebs (DE-588)4012122-7 s Carcinogenese (DE-588)4069853-1 s Mitochondriale DNS (DE-588)4202301-4 s Mutation (DE-588)4170883-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022220029&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Akouchekian, Mansoureh 1968- Investigation of human mitochondrial DNA abnormality in colon cancer Dickdarmkrebs (DE-588)4012122-7 gnd Mitochondriale DNS (DE-588)4202301-4 gnd Mutation (DE-588)4170883-0 gnd Carcinogenese (DE-588)4069853-1 gnd |
| subject_GND | (DE-588)4012122-7 (DE-588)4202301-4 (DE-588)4170883-0 (DE-588)4069853-1 (DE-588)4113937-9 |
| title | Investigation of human mitochondrial DNA abnormality in colon cancer |
| title_auth | Investigation of human mitochondrial DNA abnormality in colon cancer |
| title_exact_search | Investigation of human mitochondrial DNA abnormality in colon cancer |
| title_full | Investigation of human mitochondrial DNA abnormality in colon cancer von Mansoureh Akouchekian |
| title_fullStr | Investigation of human mitochondrial DNA abnormality in colon cancer von Mansoureh Akouchekian |
| title_full_unstemmed | Investigation of human mitochondrial DNA abnormality in colon cancer von Mansoureh Akouchekian |
| title_short | Investigation of human mitochondrial DNA abnormality in colon cancer |
| title_sort | investigation of human mitochondrial dna abnormality in colon cancer |
| topic | Dickdarmkrebs (DE-588)4012122-7 gnd Mitochondriale DNS (DE-588)4202301-4 gnd Mutation (DE-588)4170883-0 gnd Carcinogenese (DE-588)4069853-1 gnd |
| topic_facet | Dickdarmkrebs Mitochondriale DNS Mutation Carcinogenese Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022220029&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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