Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells: role of myosin light chain phosphatase
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2007
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | V, 93 Bl. Ill., graph. Darst. |
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
LIST OF ABBREVIATIONS 1
1 INTRODUCTION 4
1.1 BARRIER FUNCTION OF THE VASCULAR ENDOTHELIUM 4
1.2 ENDOTHELIAL ACTOMYOSIN CYTOSKELETON 6
1.3 ENDOTHELIAL CONTRACTILE MACHINERY 7
1.3.1 MYOSIN LIGHT CHAINS 7
1.3.2 MYSIN LIGHT CHAIN KINASE 8
1.3.3 MYOSIN LIGHT CHAIN PHOSPHATASE 9
1.3.4 REGULATION OF MYOSIN LIGHT CHAIN PHOSPHATASE ACTIVITY
VIA MYPT1 PHOSPHORYLATION 10
1.3.5 REGULATION OF MYOSIN LIGHT CHAIN PHOSPHATASE ACTIVITY BY
CPI-17 11
1.4 CAMP AND ENDOTHELIAL PERMEABILITY 12
1.5 AIMS AND OBJECTIVES OF THE STUDY 14
2 METHODS 16
2.1 CELL CULTURE 16
2.2 GENERAL INCUBATION CONDITIONS 17
2.3 PROTEIN ANALYSIS 18
2.4 DETERMINATION OF MYOSIN LIGHT CHAIN PHOSPHORYLATION 22
2.5 CO-IMMUNOPRECIPITATION OF PROTEINS 23
2.6 PROTEIN PHOSPHATASE ASSAY 24
2.7 DETECTION OF ACTIVATED RHOA 25
2.8 MEASUREMENT OF RHOA TRANSLOCATION 26
2.9 MEASUREMENT OF ENDOTHELIAL MONOLAYER PERMEABILITY 26
2.10 DOWNREGULATION OFENDOGENOUS CPI-17 27
2.11 STATISTICAL ANALYSIS 28
3. RESULTS 29
3.1 EFFECT OF FORSKOLIN ON ENDOTHELIAL PERMEABILITY 29
3.2 EFFECT OF FORSKOLIN ON MYOSIN LIGHT CHAIN PHOSPHORYLATION 30
3.3 EFFECT OF FORSKOLIN ON MLC PHOSPHATASE COMPLEX FORMATION
AND ACTIVITY 34
3.4 EFFECT OF FORSKOLIN ON MYPT 1 PHOSPHORYLATION 38
GESCANNT DURCH
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/987804200
DIGITALISIERT DURCH
IMAGE 2
3.5 EFFECT OF FORSKOLIN ON RHOA TRANSLOCATION 40
3.6 EFFECT OF FORSKOLIN ON CPI-17 PHOSPHORYLATION AND
CO-IMMUNOPRECIPITATION WITH PP1 42
3.7 EFFECT OF CPI-17 DOWNREGULATION ON ENDOTHELIAL BARRIER 48
4 DISCUSSION 50
4.1 MAIN FINDINGS 50
4.2 MLC PHOSPHATASE HOLOENZYME COMPLEX FORMATION AND ACTIVATION 51
4.3 FORSKOLIN INDUCED MLC PHOSPHATASE COMPLEX FORMATION IS
INDEPENDENT OF RHOA/ROCK INHIBITION 52
4.4 MYPT1 DEPHOSPHORYLATION 54
4.5 CAMP/PKA SIGNALING INACTIVATES CPI-17 55
4.6 CPI-17 AS A MEDIATOR OF THROMBIN INDUCED BARRIER FAILURE 57
4.7 FUTURE PERSPECTIVE 59
5 REFERENCES 60
6 SUMMARY 76
7 ZUSAMMENFASSUNG 77
8 ERKLAERUNG 78
9 APPENDIX 79
9. 1 CHEMICALS AND CONSUMABLES 79
9.2 ANTIBODIES 82
9.3 LABORATORY INSTRUMENTS 83
9.4 SOLUTIONS 84
10 ACKNOWLEDGMENT 87
11 CURRICULUM VITAE 88
12 PUBLICATIONS 89
13 PUBLISHED ABSTRACTS 90
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| any_adam_object | 1 |
| author | Aslam, Muhammad |
| author_facet | Aslam, Muhammad |
| author_role | aut |
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| building | Verbundindex |
| bvnumber | BV026629589 |
| ctrlnum | (OCoLC)918316657 (DE-599)DNB987804200 |
| dewey-full | 612.01575 |
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| dewey-ones | 612 - Human physiology |
| dewey-raw | 612.01575 |
| dewey-search | 612.01575 |
| dewey-sort | 3612.01575 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| spelling | Aslam, Muhammad Verfasser aut Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase vorgelegt von Muhammad Aslam 2007 V, 93 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Gießen, Univ., Diss., 2007 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022182037&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Aslam, Muhammad Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase |
| subject_GND | (DE-588)4113937-9 |
| title | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase |
| title_auth | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase |
| title_exact_search | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase |
| title_full | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase vorgelegt von Muhammad Aslam |
| title_fullStr | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase vorgelegt von Muhammad Aslam |
| title_full_unstemmed | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase vorgelegt von Muhammad Aslam |
| title_short | Effect of cAMP PKA signaling mechanism on barrier function of cultured endothelial cells |
| title_sort | effect of camp pka signaling mechanism on barrier function of cultured endothelial cells role of myosin light chain phosphatase |
| title_sub | role of myosin light chain phosphatase |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022182037&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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