Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro: the role of NF-kappa-B
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
Mensch-und-Buch-Verl.
2002
|
| Series: | Forschungsberichte aus der Humanmedizin
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IV, 101 S. graph. Darst. |
| ISBN: | 389820295X |
Staff View
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| 245 | 1 | 0 | |a Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro |b the role of NF-kappa-B |c presented by Jing Shang |
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Record in the Search Index
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| adam_text | Table of contents
1. Introduction 1
1.1. Melanogenesis 1
1.1.1. Pigmentation 1
1.1.2. Pigment genes 4
1.1.3. Regulation of melanogenesis 5
1.2. Transcription factor of melanogenesis 7
U. Photoprotective action of melanogenesis 8
1.3.1. Effects ofUVA on epidermis 8
1.3.2. Sun-induced skin pigmentation 9
1.3.3. Direct effects of UV on melanocytes 10
1.3.4. Indirect effects of UV 10
1.3.5. Melanin plays a crucial role in photoprotection 11
1.3.6. Antioxidant effects on pigmentation 12
1.4. NFkB system 12
1.4.1. NFkB family 12
1.4.2. Effects of oxidants and antioxidants on NFkB IS
1.5. Role of NFkB in skin 16
1.6. Aim of this work 17
2. Material and Methods 18
2.1. Materials 18
2.1.1. Equipments 18
2.1.2. Cell lines 19
2.1.3. Reagents 19
2.1.4. Radioactive agent 20
2.1.5. Antibodies 20
2.1.6. Probes 21
2.1.7. Cell culture materials 21
2.2. Methods 22
2.2.1. Growth medium and solutions used for cultivation 22
2.2.2. Cell cultivation 22
2.2.3. Treatment with different agents in vitro 24
2.2.4. Cytotoxicity assay 24
2.2.5. Proliferation assay 25
2.2.6. Nuclear protein extraction 26
2.2.7. Determination of protein concentration 27
2.2.8. Electrophoretic mobility shift assay (EMSA) 28
2.2.9. Isolation of total RNA 29
2.2.10. Northern blotting 30
2.2.11. Tyrosinase activity assay 31
2.2.12. Measurement of the melanin content 33
3. Results 34
3.1. Cytotoxicity of different agents on NHM 34
and melanoma cell lines
3.1.1. Cytotoxicity of UVA 34
3.1.2. Cytotoxic effect of L-ascorbic acid 37
3.2. Growth of NHM and of melanoma cell lines 38
in vitro after treatment with different agents
38
3.2.1. Proliferation curves
3.2.2. Action ofUVA on cell proliferation
3.2.3. Proliferation of different cell lines treated with L-ascorbic acid
33. NFkB binding activiryof cell lines in vitro after
treatment with different agents
3.3.1. Measurements in NHM 46
in comparison to untreated control
3.3.2. Measurements in melanoma cell lines 48
3.3.3. Measurements in NHM treated with supernatant from 51
UVA-irradiated HaCaT cells
3.4. Pigment gene expression after treatment with various agents 54
3.4.1. NHM 54
3.4.2. Melanoma cell lines 57
3.5. Analysis of tyrosinase activity 60
3.5.1. NHM 60
3.5.2. Melanoma cell lines 62
3.6. Detection of melanin content 63
3.6.1. NHM 63
3.6.2. Melanoma cell lines 64
3.7. Comparison of NFkB binding activity and melanin synthesis 66
3.7.1. NHM 66
3.7.2. Melanoma cell lines 67
4. Discussion 69
4.1. UVA effects on melanogenesis and NFkB binding activity 69
4.2 Effects of L-ascorbic acid on melanogenesis and 75
NFkB binding activity
43. Role of NFkB and various cytokines in the melanogenesis 77
5. Summary 82
6. Bibliography 84
7. Curriculum vitae 97
in
8. Publications 99
9. Abbreviations 100
Acknowledgements 101
IV
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| indexdate | 2024-07-09T23:11:29Z |
| institution | BVB |
| isbn | 389820295X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-021988560 |
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| physical | IV, 101 S. graph. Darst. |
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| series2 | Forschungsberichte aus der Humanmedizin |
| spelling | Shang, Jing Verfasser aut Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B presented by Jing Shang Berlin Mensch-und-Buch-Verl. 2002 IV, 101 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Forschungsberichte aus der Humanmedizin Berlin, Freie Univ., Diss., 2001 Nuklearfaktor Kappa B (DE-588)4348985-0 gnd rswk-swf Biosynthese (DE-588)4006902-3 gnd rswk-swf Ultraviolett (DE-588)4186733-6 gnd rswk-swf Melanin (DE-588)4169364-4 gnd rswk-swf Melanophor (DE-588)4264165-2 gnd rswk-swf Krebszelle (DE-588)4128704-6 gnd rswk-swf Melanom (DE-588)4074707-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Melanom (DE-588)4074707-4 s Krebszelle (DE-588)4128704-6 s Melanin (DE-588)4169364-4 s Biosynthese (DE-588)4006902-3 s Nuklearfaktor Kappa B (DE-588)4348985-0 s Ultraviolett (DE-588)4186733-6 s DE-188 Melanophor (DE-588)4264165-2 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021988560&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Shang, Jing Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B Nuklearfaktor Kappa B (DE-588)4348985-0 gnd Biosynthese (DE-588)4006902-3 gnd Ultraviolett (DE-588)4186733-6 gnd Melanin (DE-588)4169364-4 gnd Melanophor (DE-588)4264165-2 gnd Krebszelle (DE-588)4128704-6 gnd Melanom (DE-588)4074707-4 gnd |
| subject_GND | (DE-588)4348985-0 (DE-588)4006902-3 (DE-588)4186733-6 (DE-588)4169364-4 (DE-588)4264165-2 (DE-588)4128704-6 (DE-588)4074707-4 (DE-588)4113937-9 |
| title | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B |
| title_auth | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B |
| title_exact_search | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B |
| title_full | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B presented by Jing Shang |
| title_fullStr | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B presented by Jing Shang |
| title_full_unstemmed | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of NF-kappa-B presented by Jing Shang |
| title_short | Regulation of UVA-induced melanin synthesis in normal human melanocytes and melanoma cells in vitro |
| title_sort | regulation of uva induced melanin synthesis in normal human melanocytes and melanoma cells in vitro the role of nf kappa b |
| title_sub | the role of NF-kappa-B |
| topic | Nuklearfaktor Kappa B (DE-588)4348985-0 gnd Biosynthese (DE-588)4006902-3 gnd Ultraviolett (DE-588)4186733-6 gnd Melanin (DE-588)4169364-4 gnd Melanophor (DE-588)4264165-2 gnd Krebszelle (DE-588)4128704-6 gnd Melanom (DE-588)4074707-4 gnd |
| topic_facet | Nuklearfaktor Kappa B Biosynthese Ultraviolett Melanin Melanophor Krebszelle Melanom Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021988560&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT shangjing regulationofuvainducedmelaninsynthesisinnormalhumanmelanocytesandmelanomacellsinvitrotheroleofnfkappab |