Nonradioactive labeling and detection of biomolecules: with 22 tables
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| Format: | Book |
| Language: | English |
| Published: |
Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo ; Hong Kong ; Barcelona ; Budapest
Springer
1992
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| Series: | Springer laboratory
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturangaben |
| Physical Description: | XXIII, 436 S. graph. Darst. |
| ISBN: | 3540554823 0387554823 |
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MARC
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| 245 | 1 | 0 | |a Nonradioactive labeling and detection of biomolecules |b with 22 tables |c C. Kessler (ed.) |
| 264 | 1 | |a Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo ; Hong Kong ; Barcelona ; Budapest |b Springer |c 1992 | |
| 300 | |a XXIII, 436 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Springer laboratory | |
| 500 | |a Literaturangaben | ||
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| 700 | 1 | |a Kessler, Christoph |4 edt | |
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CONTENTS
1 GENERAL ASPECTS OF NONRADIOACTIVE LABELING
AND DETECTION' (C. KESSLER) 1
1.1 INTRODUCTION 1
1.2 THE CONCEPT OF NONRADIOACTIVE BIOANALYTICS .
.
. 2
1.3 DIRECT AND INDIRECT LABELING AND DETECTION SYSTEMS 4
1.3.1 DIRECT SYSTEMS 5
1.3.2 INDIRECT SYSTEMS 5
1.4 LABELING METHODS 11
1.4.1 ENZYMATIC LABELING 12
1.4.2 PHOTOCHEMICAL LABELING 13
1.4.3 CHEMICAL LABELING 13
1.5 DETECTION METHODS 16
1.5.1 DIRECT DETECTION SYSTEMS 16
1.5.2 INDIRECT DETECTION SYSTEMS 18
1.5.2.1 OPTICAL DETECTION 18
1.5.2.2 LUMINESCENCE DETECTION 19
1.5.2.3 FLUORESCENCE DETECTION 20
1.6 GUIDE OF THE USE OF INFORMATION IN THIS BOOK .
.
. 23
1 STANDARD NONRADIOACTIVE LABELING
AND DETECTION SYSTEMS
2 OVERVIEW OF NONRADIOACTIVE LABELING SYSTEMS
(C. KESSLER) 27
3 THE DIGOXIGENIN: ANTI-DIGOXIGENIN (DIG)
SYSTEM 35
3.1 OVERVIEW (C. KESSLER) 35
3.2 LABELING AND DETECTION OF NUCLEIC ACIDS
(H.-J. HOLTKE, R. SEIBL, G. G. SCHMITZ, T. WALTER,
R. RUGER, G. SAGNER, J. BURG, K. MUHLEGGER,
AND C. KESSLER) 36
3.2.1 PRINCIPLE AND APPLICATIONS 36
HTTP://D-NB.INFO/921003072
X CONTENTS
3.2.2 REACTION SCHEME 38
3.2.3 RANDOM-PRIMED LABELING WITH DIG-[LL]-DUTP
AND KLENOW ENZYME 38
3.2.4 PCR-GUIDED SYNTHESIS OF VECTOR-FREE,
DIG-LABELED DNA PROBE WITH DIG-[LL]-DUTP . 40
3.2.5 RNA LABELING BY RUN-OFF TRANSCRIPTION WITH
DIG-[11]-UTP AND SP6, T7 OR T3 RNA
POLYMERASE 42
3.2.6 01IGONUCLEOTIDETAILINGWITHDIG-[LL]-DUTP/DATP
AND TERMINAL TRANSFERASE 43
3.2.7 OLIGONUCLEOTIDE 3' END LABELING WITH
DIG-[LL]-DDUTP AND TERMINAL TRANSFERASE .
.
. 45
3.2.8
PHOTOLABELING OFF DNA OR RNA
WITH PHOTODIG 46
3.2.9 HYBRIDIZATION WITH DIG-LABELED DNA
OR DIG-LABELED OLIGONUCLEOTIDES 46
3.2.10 HYBRIDIZATION WITH DIG-LABELED RNA 49
3.2.11 OPTICAL DETECTION OF DIG MODIFICATION
WITH BCIP/NBT 50
3.2.12 CHEMILUMINESCENT DETECTION OF DIG MODIFICATION
WITH LUMIGENYY PPD OR LUMIPHOSYY 530 51
3.2.13 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 53
3.3 LABELING AND DETECTION OF PROTEINS AND
GLYCOPROTEINS (A. HASELBECK AND W. HOSEL) .
.
. 56
3.3.1
PRINCIPLE AND APPLICATIONS 56
3.3.2 LABELING AND DETECTION OF PROTEINS/PEPTIDES .
.
. 56
3.3.3
LABELING AND DETECTION OF GLYCOCONJUGATES .
.
.
. 57
3.3.4
GENERAL LABELING AND DETECTION OF PROTEINS/
PEPTIDES WITH DIG-ESTER AND DIG-MALEIMIDE . . 57
3.3.5 SELECTIVE LABELING OF SULFHYDRYL GROUPS 60
3.3.6 SELECTIVE LABELING OF DISULFIDES 61
3.3.7 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 61
3.3.8 GENERAL LABELING AND DETECTION OF GLYCOCONJUGATES
WITH DIG-HYDRAZIDE 62
3.3.9 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 64
3.3.10 SIALIC ACID-SPECIFIC OXIDATION 65
3.3.11 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 66
3.3.12 SELECTIVE DETECTION OF TERMINAL GALACTOSE RESIDUES 66
3.3.13 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 66
3.3.14 LABELING OF GLYCOCONJUGATES WITH DIG-LECTINS . . 67
3.3.15 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 68
4 THE BIOTIN SYSTEM 70
4.1 LABELING AND DETECTION OF NUCLEIC ACIDS
(A. RASHTCHIAN AND J. MACKEY) 70
4.1.1 PRINCIPLE AND APPLICATIONS 70
CONTENTS X
I
4.1.1.1 LABELING OF NUCLEIC ACIDS WITH BIOTIN 71
4.1.1.2 BIOTINYLATED NUCLEOTIDES 71
4.1.2 METHODS OF LABELING DNA 73
4.1.2.1 BIOTIN LABELING OF DNA BY NICK TRANSLATION .
.
. 73
4.1.2.2 RANDOM PRIMER LABELING OF DNA WITH BIOTIN . . 75
4.1.2.3 POLYMERASE CHAIN REACTION 77
4.1.2.4 LABELING WITH PHOTOBIOTIN 79
4.1.3 LABELING OF SYNTHETIC OLIGONUCLEOTIDES 80
4.1.3.1 ENZYMATIC LABELING 80
4.1.3.2 CHEMICAL SYNTHESIS OF BIOTINYLATED
OLIGONUCLEOTIDES ' 82
4.1.4 METHODS OF LABELING RNA 82
4.1.5 HYBRIDIZATION AND DETECTION OF BIOTINYLATED PROBES 84
4.1.5.1 HYBRIDIZATION 85
4.1.5.2 HYBRIDIZATION OF SYNTHETIC OLIGONUCLEOTIDE PROBES 86
4.1.5.3 BINDING THE STREPTAVIDIN-ALKALINE PHOSPHATASE
CONJUGATE 87
4.1.5.4 CHROMOGENIC DETECTION OF BIOTINYLATED PROBES
WITH ALKALINE PHOSPHATASE 87
4.1.5.5 CHEMILUMINESCENT DETECTION OF BIOTINYLATED
PROBES WITH ALKALINE PHOSPHATASE 88
4.1.6 SUMMARY 88
4.2 LABELING AND DETECTION OF PROTEINS
AND GLYCOPROTEINS (E. A. BAYER AND M. WILCHEK) 91
4.2.1 PRINCIPLE AND APPLICATIONS 91
4.2.2 BIOTINYLATION OF PROTEINS VIA LYSINES 92
4.2.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 93
4.2.4 BIOTINYLATION OF PROTEINS VIA CYSTEINES 94
4.2.5 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 95
4.2.6 BIOTINYLATION OF PROTEINS VIA TYROSINES OR HISTIDINES 95
4.2.7 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 96
4.2.8 BIOTINYLATION OF PROTEINS VIA ASPARTATIC AND
GLUTAMIC ACIDS 96
4.2.9 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 97
4.2.10 BIOTINYLATION OF GLYCOPROTEINS VIA SUGAR RESIDUES 98
4.2.11 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 99
5 IN VIVO LABELING OF DNA PROBES WITH 5-BRDU
(J. L. GUESDON) 101
5.1 PRINCIPLE AND APPLICATIONS 101
5.2 REACTION SCHEME 103
5.3 IN VIVO RECOMBINANT M13 DNA LABELING
WITH 5-BRDU, HYBRIDIZATION,
AND IMMUNOENZYMATIC DETECTION 103
5.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 107
XII CONTENTS
6 THE SULFONE SYSTEM (I. NUR AND M. HERZBERG) . 110
6.1 PRINCIPLE AND APPLICATIONS 110
6.2 THE PHOTO-CHEMIPROBE PROCEDURE:
LABELING BY SULFONATION, HYBRIDIZATION,
AND CHEMILUMINESCENCE VISUALIZATION I
L
L
6.2.1 BCIP/NBT AS AN ALTERNATIVE CHROMOGENIC SYSTEM 113
6.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 113
6.4 LABELING OF OLIGONUCLEOTIDE PRIMERS
FOR USE IN PCR AND THE USE OF AMPLIFIED PRODUCTS
AS A LABELED PROBE 114
7 COLLOIDAL GOLD AS A MARKER IN MOLECULAR BIOLOGY:
THE USE OF ULTRA-SMALL GOLD PROBES
(P. F. E. M. VANDEPLAS AND J. L. M. LEUNISSEN) . 116
7.1 PRINCIPLE AND APPLICATIONS 116
7.1.1 LIMITATIONS OF THE GOLD MARKER SYSTEM 117
7.1.2 IMPROVEMENT BY REDUCTION OF PARTICLE SIZE .
.
.
.
117
7.2 REACTION SCHEME 118
7.3 INDIRECT IMMUNOGOLD SILVER STAINING 119
7.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 121
7.5 SELECTED EXAMPLE: IMMUNODETECTION
OF GONADATROPIC HORMONE IN CATFISH PITUITARY . . 123
8 DIRECT PEROXIDASE LABELING OF HYBRIDIZATION
PROBES AND CHEMILUMINESCENCE DETECTION
(I. DURRANT) 127
8.1 PRINCIPLE AND APPLICATIONS 127
8.2 REACTION SCHEME 129
8.3 DIRECTLY LABELED NUCLEIC ACID (LONG) PROBES . . 130
8.4 OLIGONUCLEOTIDE PROBES 131
8.5 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 132
9 A HIGHLY SENSITIVE METHOD FOR DETECTING
PEROXIDASE IN IN SITU HYBRIDIZATION
OR IMMUNOHISTOCHEMICAL ASSAYS
(J. G. LAZAR AND F. E. TAUB) 135
9.1 PRINCIPLE AND APPLICATIONS 135
9.2 REACTION SCHEME 138
9.3 IN SITU DETECTION OF PEROXIDASE 138
9.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 141
10 THE SNAP SYSTEM (J. E.MARICH AND J. L.RUTH) . 143
10.1 PRINCIPLE AND APPLICATIONS 143
10.2 COLORIMETRIC DETECTION OF MYCOBACTERIA ON FILTERS 144
CONTENTS XIII
10.3 COLORIMETRIC DETECTION OF HUMAN
PAPILLOMAVIRUS IN SITU 145
10.4 CHEMILUMINESCENT DETECTION OF HUMAN
DNA FINGERPRINTS 146
10.5 FLUORESCENCE DETECTION OF HUMAN
IMMUNODEFICIENCY VIRUS USING SANDWICH
HYBRIDIZATION 147
II
SPECIALIZED NONRADIOACTIVE
DETECTION SYSTEMS
11 OVERVIEW OF COLORIMETRIC, CHEMILUMINOMETRIC,
AND FLUORIMETRIC DETECTION SYSTEMS
(H.-J. GUDER AND H.-P. JOSEL) 153
11.1 PRINCIPLE AND APPLICATIONS 153
11.2 SUBSTRATES FOR HYDROLASES 154
11.2.1 DIRECT SUBSTRATES 154
11.2.2 INDIRECT SUBSTRATES 155
11.3 SUBSTRATES FOR PEROXIDASE 155
11.4 APPLICATIONS OF DIRECT LABELS 156
12 COLORIMETRIC SYSTEMS 159
12.1 INDIGO/TETRAZOLIUM DYES (H.-J. GUDER) 159
12.1.1 PRINCIPLE AND APPLICATIONS 159
12.1.2 DETECTION WITH BCIP/NBT 161
12.2 AZO DYES -(W. KUNZ AND S. WEST) 161
12.2.1 PRINCIPLE AND APPLICATIONS 161
12.2.2 DETECTION WITH AZO DYES 163
12.2.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 164
13 LUMINESCENCE SYSTEMS 165
13.1 CHEMILUMINESCENCE: LUMINOL (H.-P. JOSEL) . . . 165
13.1.1 PRINCIPLE AND APPLICATIONS 165
13.1.2 CHEMILUMINESCENT LABELING AND DETECTION
WITH HORSERADISH PEROXIDASE AND LUMINOL .
.
.
.
166
13.1.3
SPECIAL HINTS
FOR APPLICATION AND TROUBLESHOOTING 167
13.2 CHEMILUMINESCENCE: PROPERTIES OF 1,2-DIOXETANE
CHEMILUMINESCENCE (I.BRONSTEINANDL.J. KRICKA) 168
13.3 ELECTROCHEMILUMINESCENCE:
RUTHENIUM COMPLEXES (J. H. KENTEN) 175
13.3.1 PRINCIPLE AND APPLICATIONS 175
13.3.2 DETECTION OF PCR PRODUCTS
USING AN ELECTROCHEMILUMINESCENCE ASSAY .
.
.
.
176
13.3.3
SPECIAL HINTS
FOR APPLICATION AND TROUBLESHOOTING 178
X
I
V CONTENTS
13.4 BIOLUMINESCENCE: LUCIFERIN
(R. E. GEIGER AND E. SCHNEIDER) 179
13.4.1 PRINCIPLE AND APPLICATIONS 179
13.4.2 REACTION SCHEME 180
13.4.3 BIOLUMINESCENCE-ENHANCED DETECTION 181
13.4.3.1 ENZYMATIC LABELING WITH ALKALINE PHOSPHATASE . . 181
13.4.3.2ENZYMATIC LABELING WITH {3-GALACTOSIDASE 182
13.4.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 182
14 FLUORESCENCE SYSTEMS 185
14.1 LABELING OF BIOMOLECULES WITH FLUORESCEIN,
RESORUFIN, AND RHODAMINE (H.-P. JOSEL) 185
14.1.1 PRINCIPLE AND APPLICATIONS 185
14.1.2 FLUORESCENT LABELING OF PROTEINS 187
14.1.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 188
14.2 TIME-RESOLVED FLUORESCENCE
(E. P. DIAMANDIS AND T. K. CHRISTOPOULOS) .
.
.
.
188
14.2.1
PRINCIPLE AND APPLICATIONS 188
14.2.2 REACTION SCHEME 189
14.2.3 DNA LABELING WITH BIOTIN, HYBRIDIZATION,
AND DETECTION 189
14.2.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 191
III ENHANCED SYSTEMS
15 OVERVIEW OF AMPLIFICATION SYSTEMS (C. KESSLER) 197
15.1 INTRODUCTION 197
15.2 TARGET AMPLIFICATION 197
15.2.1 REPLICATION 197
15.2.2 TRANSCRIPTION 200
15.3 SIGNAL AMPLIFICATION 200
15.3.1 COUPLING OF THE BINDING PARTNERS 201
15.3.2 ANTIBODY TREES AND PROBE "XMAS TREES"/
PROBE "BRUSHES" 201
15.3.3 ENZYME/POLYENZYME AMPLIFICATION 202
15.3.4 COUPLED SIGNAL CASCADES 202
15.4 TARGET-SPECIFIC SIGNAL AMPLIFICATION 202
15.4.1 REPLICATION 202
15.4.2 SELECTIVE COMPLEX SEPARATION 202
15.5 IN VIVO AMPLIFICATION . . 203
CONTENTS X
V
16 ENHANCED SIGNAL GENERATION
BY TARGET AMPLIFICATION 206
16.1 POLYMERASE CHAIN REACTION AMPLIFICATION
(R. RIIGER) 206
16.1.1 PRINCIPLE AND APPLICATIONS 206
16.1.2 DIG LABELING BY PCR AMPLIFICATION 209
16.1.3 FURTHER PROCESSING OF DIG-LABELED PCR PRODUCTS 210
16.1.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 211
16.2 AMPLIFICATION OF NUCLEIC ACID SEQUENCES
BY THE REPAIR CHAIN REACTION (D. SEGEV) .
.
.
.
212
16.2.1
PRINCIPLE AND APPLICATIONS 212
16.2.2 DETECTION OF HPV16 IN CASKI CELL LINE DNA
AND IN CLINICAL BIOPSY TARGETS 212
16.2.3 SUMMARY 217
16.3 ISOTHERMAL AMPLIFICATION OF DNATARGETS
BY STRAND DISPLACEMENT AMPLIFICATION
(M. C. LITTLE, J. G. NADEAU, G. T. WALKER,
J. L. SCHRAM, M. S. FRAISER, A. ALEXANDER,
ANDD. P. MALINOWSKI) 218
16.3.1 PRINCIPLE AND APPLICATIONS 218
16.3.2 REACTION SCHEME 220
16.3.3 STRAND DISPLACEMENT AMPLIFICATION 220
16.3.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 222
16.4 LIGASE CHAIN REACTION
(G. H. SHIMER JR. AND K. C. BACKMAN) 223
16.4.1 PRINCIPLE AND APPLICATIONS 223
16.4.2 LIGASE CHAIN REACTION ASSAY 225
16.4.3 NONRADIOACTIVE DETECTION OF AMPLIFICATION
PRODUCTS 227
16.5 ISOTHERMAL TARGET AMPLIFICATION (E.JAMES) . . . 228
16.5.1 PRINCIPLE AND APPLICATIONS 228
16.5.2 NUCLEIC ACID SEQUENCE BASED AMPLIFICATION:
GENERAL PROCEDURE 231
16.5.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 232
16.6 THE POTENTIAL OF RDNA IN IDENTIFICATION AND
DIAGNOSTICS (E. STACKEBRANDT AND W. LIESACK) 232
16.6.1 PRINCIPLE AND APPLICATIONS 232
16.6.2 ISOLATION OF GENOMIC DNA FROM PURE CULTURES . . 234
16.6.3 ISOLATION OF GENOMIC DNA FROM MIXED CULTURES
AND ENVIRONMENTAL SAMPLES 235
16.6.4 PCR-MEDIATED AMPLIFICATION OF 16S RDNA . . . 235
16.6.5 AMPLIFICATION OF 16S RDNA FROM GENES 235
XVI CONTENTS
17 SIGNAL AMPLIFICATION SYSTEM:
SUBSTRATE CASCADE
(R. I. CAN, F. K. WONG, AND D. SADI) 240
17.1 PRINCIPLE AND APPLICATIONS 240
17.2 AMPLIFICATION OF ALKALINE PHOSPHATASE SUBSTRATES 240
17.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 241
IV APPLICATION FORMATS
18 OVERVIEW OF APPLICATION FORMATS (C. KESSLER) . 247
19 BLOT FORMATS: NUCLEIC ACIDS 253
19.1 FACTORS INFLUENCING NUCLEIC ACID HYBRIDIZATION
(C. KESSLER) 253
19.2 DOT, SOUTHERN, AND NORTHERN BLOTS
(B. RIIGER AND C. KALETTA) 257
19.2.1 PRINCIPLE AND APPLICATIONS 257
19.2.2 PREPARATION OF SOUTHERN AND NORTHERN BLOTS . . . 257
19.2.3 SOUTHERN BLOT HYBRIDIZATION 259
19.2.3.ISPECIAL ASPECTS OF SOUTHERN BLOT HYBRIDIZATION . 262
19.2.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 263
19.2.5 NORTHERN BLOT HYBRIDIZATION 264
19.2.6 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 265
19.2.7 DOT BLOT HYBRIDIZATION 265
19.2.8 HYBRIDIZATION WITH OLIGONUCLEOTIDES
IN SOUTHERN AND NORTHERN BLOTTING 265
19.2.8.LHYBRIDIZATION IN TETRAMETHYLAMMONIUM CHLORIDE 266
19.3 COLONY AND PLAQUE HYBRIDIZATION (T.WALTER) . . 267
19.3.1 PRINCIPLE AND APPLICATIONS 267
19.3.2 METHODS 267
19.3.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 269
19.3.4 EXAMPLE 269
19.4 MULTILOCUS DNA FINGERPRINTING USING
NONRADIOACTIVELY LABELED OLIGONUCLEOTIDE PROBES
SPECIFIC FOR SIMPLE REPEAT ELEMENTS
(J. T. EPPLEN AND J. MDTHE) 271
19.4.1 PRINCIPLE AND APPLICATIONS 271
19.4.2 DEMONSTRATION OF DNA FINGERPRINTS
BY DIG-LABELED OLIGONUCLEOTIDES 273
19.4.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 276
19.5 TN5CCS RESTRICTION MAPPING OF LARGE DNA
PLASMIDS (U. ZUBER AND W. SCHUMANN) 277
19.5.1 PRINCIPLE AND APPLICATIONS 277
19.5.2 REACTION SCHEME 278
19.5.3 TN5COS RESTRICTION MAPPING 278
CONTENTS XVII
19.6 DIG DNA SEQUENCING WITH "CHEMILUMINESCENT
OR DYE SUBSTRATE (G. SAGNER) 281
19.6.1 PRINCIPLE AND APPLICATIONS 281
19.6.2 DIG DNA SEQUENCING AND DETECTION 282
19.6.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 287
19.7 DNA SEQUENCING: CHEMILUMINESCENT DETECTION
WITH THE 1,2-DIOXETANE CSPD
(C. S. MARTIN AND I. BRONSTEIN) 288
19.7.1 PRINCIPLE AND APPLICATIONS 288
19.7.2 DNA SEQUENCING AND DETECTION WITH CSPD . . . 289
19.7.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 292
19.8 DIRECT BLOTTING ELECTROPHORESIS
FOR DNA SEQUENCING (T. M. POHL) 293
19.8.1 PRINCIPLE AND APPLICATIONS 293
19.8.2 DNA SEQUENCING BY DIRECT BLOTTING
ELECTROPHORESIS 294
20 BLOT FORMATS: PROTEINS AND GLYCOPROTEINS . . . 297
20.1 DETECTION OF PROTEINS AND GLYCOPROTEINS
ON WESTERN BLOTS (A. HASELBECK AND W. HOSEL) . 297
20.1.1 DETECTION OF PROTEINS 297
20.1.2 DETECTION OF GLYCOPROTEINS 298
20.2 SOUTHWESTERN ANALYSIS USING DIG (S. DOOLEY) . 299
20.2.1 PRINCIPLE AND APPLICATIONS 299
20.2.2 DIG SOUTHWESTERN ANALYSIS 299
20.2.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 302
21 IN SITU FORMATS 304
21.1 VIRUS DETECTION IN FIXED CELLS
(E. GENERSCH, B. J. HEILES AND R. NEUMANN) . . . 304
21.1.1 PRINCIPLE AND APPLICATIONS 304
21.1.2 DETECTION OF VIRAL SEQUENCES IN FIXED CELLS
AND TISSUES BY IN SITU HYBRIDIZATION 305
21.1.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 306
21.2 DIFFERENTIATION OF VIRAL AND CHROMOSOMAL NUCLEIC
ACIDS IN INDIVIDUAL NUCLEI (C. S. HERRINGTON) . . . 307
21.2.1 PRINCIPLE AND APPLICATIONS 307
21.2.2 NON-FLUORESCENT IN SITU HYBRIDIZATION 309
21.2.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 314
21.3 VIRUS DETECTION IN BIOPSY SPECIMENS
(P. HEINO AND V. HUKKANEN) 316
21.3.1 PRINCIPLE AND APPLICATIONS 316
21.3.2 REACTION SCHEME 317
XVIII CONTENTS
21.3.3 IN SITU HYBRIDIZATION OF TISSUE SPECIMENS
WITH DIG-[LL]-DUTP-LABELED DNA
AND WITH DIG-[11]-UTP-LABELED RNA PROBES . . 317
21.3.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 322
21.4 FLUORESCENT IN SITU HYBRIDIZATION ON BANDED
CHROMOSOMES (N. ARNOLD, M. BHATT, T. RIED,
J. WIENBERG AND D. C. WARD) 324
21.4.1 PRINCIPLE AND APPLICATIONS 324
21.4.2 DETAILED STANDARD PROCEDURE 325
21.4.3 GENERAL PROTOCOL 327
21.4.4 CHROMOSOME BANDING PROTOCOLS 329
21.5 PROBE LABELING AND HYBRIDIZATION IN ONE STEP
(J.KOCH) 336
21.5.1 PRINCIPLE AND APPLICATIONS 336
21.5.2 PROCEDURE 338
21.5.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 340
21.6 MULTIPLE FLUORESCENCE IN SITU HYBRIDIZATION
FOR MOLECULAR CYTOGENETICS
(A. K. RAAP, J. WIEGANT, AND P. LICHTER) 343
21.6.1 PRINCIPLE AND APPLICATIONS 343
21.6.2 REACTION SCHEME 344
21.6.3 MULTIPLE FLUORESCENCE IN SITU HYBRIDIZATION . . . 344
21.6.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 351
21.7 MAPPING OF POLYTENE CHROMOSOMES
(E. R. SCHMIDT) 354
21.7.1 PRINCIPLE AND APPLICATIONS 354
21.7.2 MAPPING OF POLYTENE CHROMOSOMES
BY MULTIPLE IN SITU HYBRIDIZATION 355
21.7.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 357
21.8 DETECTION OF MRNA IN FIXED CELLS
WITH DIG-LABELED RNA PROBES
(A. STARZINSKI-POWITZ AND K. ZIMMERMANN) . . . 359
21.8.1 PRINCIPLE AND APPLICATIONS 359
21.8.2 DIG-LABELED RNA PROBES IN THE DETECTION
OF MRNA IN FIXED CELLS 360
21.9 DETECTION OF MRNA IN FIXED TISSUES
USING RNA PROBES (K.J.HILLAN) 363
21.9.1 PRINCIPLE AND APPLICATIONS 363
21.9.2 STANDARD PROCEDURE 364
21.9.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 366
21.10 DETECTION OF MRNA IN SITU WITH DIG-LABELED
SYNTHETIC OLIGONUCLEOTIDE PROBES
(F. BALDINO JR., E. ROBBINS, AND M. E. LEWIS) . . 367
CONTENTS X
I
X
21.10.1 PRINCIPLE AND APPLICATIONS 367
21.10.2 DIG-LABELED SYNTHETIC OLIGONUCLEOTIDE PROBES
TO DETECT MRNA IN SITU 369
21.10.3 RESULTS AND CONCLUDING REMARKS 372
21.11 WHOLE MOUNT IN SITU HYBRIDIZATION FOR THE DETECTION
OF MRNA IN
DROSOPHILA
EMBRYOS (D. TAUTZ) . . . 373
21.11.1 PRINCIPLE AND APPLICATIONS 373
21.11.2 WHOLE MOUNT IN SITU HYBRIDIZATION 374
21.11.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 376
21.12 DIG-LABELED SINGLE-STRANDED DNA PROBES
FOR IN SITU HYBRIDIZATION
(N. H. PATEL AND C. S. GOODMAN) 377
21.12.1 PRINCIPLE AND APPLICATIONS 377
21.12.2 DIG-LABELED SINGLE-STRANDED DNA PROBES . . . 378
21.12.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 380
21.13 DOUBLE LABELING OF MRNA AND PROTEINS IN
DROSOPHILA
EMBRYOS (B. COHEN AND S.M. COHEN) 382
21.13.1 PRINCIPLE AND APPLICATIONS 382
21.13.2 CHOICE OF SIGNAL DETECTION SYSTEM 383
21.13.3 DOUBLE LABELING OF MRNA AND PROTEINS 384
21.13.3.1 WHOLE MOUNT IN SITU HYBRIDIZATION 386
21.13.3.2 X-GAL REACTION FOLLOWED BY IN SITU HYBRIDIZATION 388
21.13.3.3 IN SITU HYBRIDIZATION
FOLLOWED BY ANTIBODY STAINING 389
21.13.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 390
22 QUANTITATIVE FORMATS 393
22.1 AFFINITY-BASED COLLECTION OF SANDWICH HYBRIDS:
A QUANTITATIVE HYBRIDIZATION FORMAT
(H. SODERLUND AND K. KORPELA) 393
22.1.1 PRINCIPLE AND APPLICATIONS 393
22.1.2 QUANTIFICATION OF HPV DNA BY AFFINITY CAPTURE . 396
22.1.3 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 397
22.2 DETECTION OF DNA:RNATARGET:PROBE COMPLEXES
WITH DNA:RNA-SPECIFIC ANTIBODIES
(F.
COUTLEE, R. H. YOLKEN, AND R. P. VISCIDI) . . 399
22.2.1 PRINCIPLE AND APPLICATIONS 399
22.2.2 REACTION SCHEME 401
22.2.3 DETECTION OF PCR-AMPLIFIED DNA
BY ENZYME IMMUNOASSAY 401
22.2.4 SPECIAL HINTS FOR APPLICATION AND TROUBLESHOOTING 405
22.3 CAPTURING OF DISPLACED DNA STRANDS
AS A DIAGNOSTIC METHOD (M. COLLINS) 407
22.3.1 PRINCIPLE AND APPLICATIONS 407
22.3.2 STRAND DISPLACEMENT ASSAY 409
X
X CONTENTS
22.4 NONRADIATIVE FLUORESCENCE RESONANCE
ENERGY TRANSFER (R. A. CARDULLO) 414
22.4.1 BASIC PRINCIPLES OF NONRADIATIVE
FLUORESCENCE RESONANCE ENERGY TRANSFER .
.
.
.
414
22.4.2
METHODOLOGIES FOR MEASURING TRANSFER EFFICIENCIES 415
22.4.3 USING ENERGY TRANSFER TO MONITOR
NUCLEIC ACID HYBRIDIZATION 418
22.4.4 SUMMARY 422
22.5 COLORIMETRIC ASSAYS WITH STREPTAVIDIN/AVIDIN
COATED SURFACES (R. SEIBL AND S. KOEHLER) . . . 424
22.5.1 PRINCIPLE AND APPLICATIONS 424
22.5.2 QUANTITATIVE DETECTION OF DIG-LABELED
NUCLEIC ACID 425
APPENDIX I SUPPLIERS OF REAGENTS 429
APPENDIX II SUPPLIERS OF EQUIPMENT 435 |
| any_adam_object | 1 |
| author2 | Kessler, Christoph |
| author2_role | edt |
| author2_variant | c k ck |
| author_facet | Kessler, Christoph |
| building | Verbundindex |
| bvnumber | BV025862347 |
| ctrlnum | (OCoLC)311503679 (DE-599)BVBBV025862347 |
| dewey-full | 574.19285 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] |
| dewey-raw | 574.19285 |
| dewey-search | 574.19285 |
| dewey-sort | 3574.19285 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| id | DE-604.BV025862347 |
| illustrated | Illustrated |
| indexdate | 2024-12-02T09:05:06Z |
| institution | BVB |
| isbn | 3540554823 0387554823 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-019109999 |
| oclc_num | 311503679 |
| open_access_boolean | |
| owner | DE-11 |
| owner_facet | DE-11 |
| physical | XXIII, 436 S. graph. Darst. |
| publishDate | 1992 |
| publishDateSearch | 1992 |
| publishDateSort | 1992 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer laboratory |
| spelling | Nonradioactive labeling and detection of biomolecules with 22 tables C. Kessler (ed.) Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo ; Hong Kong ; Barcelona ; Budapest Springer 1992 XXIII, 436 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer laboratory Literaturangaben Markierung Chemie (DE-588)4201779-8 gnd rswk-swf Biomolekül (DE-588)4135124-1 gnd rswk-swf Biomolekül (DE-588)4135124-1 s Markierung Chemie (DE-588)4201779-8 s DE-604 Kessler, Christoph edt DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019109999&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nonradioactive labeling and detection of biomolecules with 22 tables Markierung Chemie (DE-588)4201779-8 gnd Biomolekül (DE-588)4135124-1 gnd |
| subject_GND | (DE-588)4201779-8 (DE-588)4135124-1 |
| title | Nonradioactive labeling and detection of biomolecules with 22 tables |
| title_auth | Nonradioactive labeling and detection of biomolecules with 22 tables |
| title_exact_search | Nonradioactive labeling and detection of biomolecules with 22 tables |
| title_full | Nonradioactive labeling and detection of biomolecules with 22 tables C. Kessler (ed.) |
| title_fullStr | Nonradioactive labeling and detection of biomolecules with 22 tables C. Kessler (ed.) |
| title_full_unstemmed | Nonradioactive labeling and detection of biomolecules with 22 tables C. Kessler (ed.) |
| title_short | Nonradioactive labeling and detection of biomolecules |
| title_sort | nonradioactive labeling and detection of biomolecules with 22 tables |
| title_sub | with 22 tables |
| topic | Markierung Chemie (DE-588)4201779-8 gnd Biomolekül (DE-588)4135124-1 gnd |
| topic_facet | Markierung Chemie Biomolekül |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019109999&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kesslerchristoph nonradioactivelabelinganddetectionofbiomoleculeswith22tables |