A laboratory guide to in vitro studies of protein-DNA interactions:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Basel ; Boston ; Berlin
Birkhäuser
1991
|
| Schriftenreihe: | BioMethods
5 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | 326 S. Ill., graph. Darst. |
| ISBN: | 3764326271 |
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Datensatz im Suchindex
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|---|---|
| adam_text |
TABLE OF CONTENTS
LIST OF CONTRIBUTING AUTHORS . 11
I INTRODUCTION . 15
THE STUDY OF PROTEIN-DNA INTERACTIONS BY MEANS OF ENZYMES
II THE STUDY OF PROTEIN-DNA INTERACTIONS BY DEOXYRIBONUCLEASE 1
FOOTPRINTING . 19
DIETMAR VON DER AHE
III EXONUCLEASE III PROTECTION ASSAY FOR SPECIFIC DNA-BINDING
PROTEINS. 35
JEAN-PIERRE JOST
IV THE PROTEOLYTIC CLIPPING BAND-SHIFT ASSAY OF PROTEIN-DNA
COMPLEXES. 45
MELYA J. HUGHES, HAIMIN LIANG AND JEAN-PIERRE JOST
THE STUDY OF PROTEIN-DNA INTERACTIONS BY UV LIGHT
V VISUALISING INTIMATE PROTEIN-DNA CONTACTS AND ALTERED DNA
STRUCTURES WITH ULTRAVIOLET LIGHT . 55
MICHAEL M. BECKER AND ZHOU WANG
VI UV CROSS-LINKING OF PROTEIN TO BROMOURIDINE-SUBSTITUTED RNA 71
IAIN J. MCEWAN
VII MOLECULAR WEIGHT DETERMINATION OF DNA-BINDING PROTEINS BY
UV CROSS-LINKING COMBINED WITH SDS POLYACRYLAMIDE GEL
ELECTROPHORESIS. 79
MELYA J. HUGHES, HAIMIN LIANG AND JEAN-PIERRE JOST
THE STUDY OF PROTEIN-DNA INTERACTIONS BY MEANS OF CHEMICALS
(INTERFERENCE ASSAYS)
VIII INTERACTION OF PROTEINS WITH PARTIALLY DEPURINATED AND
DEPYRIMIDINATED OLIGONUCLEOTIDES (MISSING-CONTACT PROBING OF
PROTEIN-DNA INTERACTIONS). 93
HAIMIN LIANG, ANDRE PAWLAK AND JEAN-PIERRE JOST
IX THE STUDY OF PROTEIN-DNA CONTACTS BY ETHYLATION INTERFERENCE . . ILL
VICHIEN RIMPHANITCHAYAKIT AND NIGEL D.F. GRINDLEY
1
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/910575703
X THE STUDY OF PROTEIN-DNA INTERACTIONS BY METHYLATION
INTERFERENCE. 121
JOSEF JIRICNY AND NATALIE CORMAN
XI USING THE CHEMISTRY OF THE HYDROXYL RADICAL TO DETERMINE
STRUCTURAL DETAILS ABOUT DNA AND PROTEIN-DNA COMPLEXES . 133
JUDITH R. LEVIN, AMANDA M. BURKHOFF AND THOMAS D. TULLIUS
XII DETECTION OF UNUSUAL DNA STRUCTURES AND DNA-PROTEIN
INTERACTIONS BY DIETHYLPYROCARBONATE CARBETHOXYLATION . 145
MIKLOS TOTH
THE STUDY OF PROTEIN-DNA INTERACTIONS BY MEANS OF ELECTRON MICROSCOPY
XIII ELECTRON MICROSCOPE VISUALISATION OF PROTEIN-DNA COMPLEXES . . 153
BEATRICE TEN HEGGELER-BORDIER AND WALTER WAHLI
THE STUDY OF PROTEIN-DNA INTERACTIONS BY GEL MOBILITY-SHIFT ASSAYS
XIV QUALITATIVE AND QUANTITATIVE STUDIES OF PROTEIN-DNA INTERACTIONS
BY GEL MOBILITY-SHIFT ASSAY. 163
STUART R. STONE, MELYA J. HUGHES AND JEAN-PIERRE JOST
XV DIAGONAL GEL MOBILITY-SHIFT ASSAYS FOR THE RESOLUTION OF
MULTI-SUBUNIT COMPLEXES BINDING TO REGULATORY ELEMENTS OF
SPECIFIC GENES. 195
FRED SCHAUFELE, BRIAN L. WEST, JEAN-PIERRE JOST AND
TIM REUDELHUBER
XVI A RAPID GEL-SHIFT TECHNIQUE TO ANALYZE DNA-PROTEIN
INTERACTIONS USING PHASTSYSTEM
YY
. 207
PONNUSAMY RAMANUJAM, SCOTT FOGERTY, WILLIAM HEISER, AND
JAMES JOLLY
PROTEIN-DNA INTERACTIONS ON A SOLID SUPPORT
XVII PURIFICATION OF SEQUENCE-SPECIFIC DNA-BINDING PROTEINS BY
AFFINITY CHROMATOGRAPHY . 221
MELYA J. HUGHES, JEAN-PIERRE JOST, AND JOSEF JIRICNY
XVIII CLONING OF SEQUENCE-SPECIFIC DNA-BINDING PROTEINS BY
SCREENING X CDNA EXPRESSION LIBRARIES WITH RADIOLABELLED
BINDING-SITE PROBES . 233
PATRICK MATTHIAS, MICHAEL M. MULLER AND WALTER SCHAFFNER
8
THE STUDY OF PROTEIN-DNA INTERACTION USING SPECIAL VECTORS
XIX DETECTION OF DNA BENDING BY GEL ELECTROPHORESIS: USE OF
PLASMID VECTORS . 245
CHRISTIAN ZWIEH AND SANKAR ADHYA
OTHER RELATED TECHNIQUES
XX THE USE OF DNA AMPLIFICATION FOR IN VITRO FOOTPRINTING
EXPERIMENTS . 259
HANSPETER SALUZ
XXI NON-RADIOACTIVE DETECTION METHODS FOR IN VITRO FOOTPRINTING . . .
277
ANDREW WALLACE AND HANSPETER SALUZ
XXII QUANTITATIVE DETERMINATION OF PROTEINS IN CRUDE EXTRACTS . 291
JAN HOFSTEENGE
APPENDICES
APPENDIX A
DNA ISOLATION FROM
E.
COLI (J.P. JOST) . 299
APPENDIX B
THE SPUN COLUMN PROCEDURE. 301
APPENDIX C
PURIFICATION OF OLIGONUCLEOTIDES (J. JIRICNY) . 303
APPENDIX D
MIGRATION OF DNA ON NATIVE AND DENATURATING GELS OF DIFFERENT
POLYACRYLAMIDE CONCENTRATION . 306
APPENDIX E
PREPARATION OF A NUCLEAR LYSATE FROM LIVER (J.P. JOST). 307
APPENDIX F
PREPARATION OF A CELL LYSATE FROM TISSUE CULTURE CELLS . 310
APPENDIX G
PREPARATION OF NUCLEAR EXTRACTS FROM CELLS IN TISSUE CULTURE. 313
APPENDIX H
INHIBITORS OF PROTEASES (E. SHAW). 316
9
APPENDIX I
THE MOST COMMON PROTEIN KINASE AND PHOSPHATASE INHIBITORS
(B. HEMMINGS) . 317
APPENDIX J
TABLES ON THE PRECIPITATION OF PROTEINS WITH AMMONIUM SULPHATE . 319
INDEX
. 321 |
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| spelling | A laboratory guide to in vitro studies of protein-DNA interactions ed. by J. P. Jost ; H. P. Saluz Basel ; Boston ; Berlin Birkhäuser 1991 326 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier BioMethods 5 Literaturangaben Proteine (DE-588)4076388-2 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf In vitro (DE-588)4250701-7 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf DNS (DE-588)4070512-2 s Proteine (DE-588)4076388-2 s Wechselwirkung (DE-588)4064937-4 s Methode (DE-588)4038971-6 s DE-604 In vitro (DE-588)4250701-7 s 1\p DE-604 Jost, Jean-Pierre edt BioMethods 5 (DE-604)BV002454363 5 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019108300&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | A laboratory guide to in vitro studies of protein-DNA interactions BioMethods Proteine (DE-588)4076388-2 gnd Methode (DE-588)4038971-6 gnd Wechselwirkung (DE-588)4064937-4 gnd In vitro (DE-588)4250701-7 gnd DNS (DE-588)4070512-2 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4038971-6 (DE-588)4064937-4 (DE-588)4250701-7 (DE-588)4070512-2 |
| title | A laboratory guide to in vitro studies of protein-DNA interactions |
| title_auth | A laboratory guide to in vitro studies of protein-DNA interactions |
| title_exact_search | A laboratory guide to in vitro studies of protein-DNA interactions |
| title_full | A laboratory guide to in vitro studies of protein-DNA interactions ed. by J. P. Jost ; H. P. Saluz |
| title_fullStr | A laboratory guide to in vitro studies of protein-DNA interactions ed. by J. P. Jost ; H. P. Saluz |
| title_full_unstemmed | A laboratory guide to in vitro studies of protein-DNA interactions ed. by J. P. Jost ; H. P. Saluz |
| title_short | A laboratory guide to in vitro studies of protein-DNA interactions |
| title_sort | a laboratory guide to in vitro studies of protein dna interactions |
| topic | Proteine (DE-588)4076388-2 gnd Methode (DE-588)4038971-6 gnd Wechselwirkung (DE-588)4064937-4 gnd In vitro (DE-588)4250701-7 gnd DNS (DE-588)4070512-2 gnd |
| topic_facet | Proteine Methode Wechselwirkung In vitro DNS |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019108300&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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