Fluorescence applications in biotechnology and life sciences:
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| Format: | Book |
| Language: | English |
| Published: |
Oxford
Wiley-Blackwell
2009
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXVII, 367 S. teilw. farb. Ill., graph. Darst. |
| ISBN: | 0470083700 9780470083703 |
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| adam_text | Titel: Fluorescence applications in biotechnology and life sciences
Autor: Goldys, Ewa M.
Jahr: 2009
^mam contents
Preface xv
Acknowledgments xxi
About the Contributing Authors xxiii
1 Basics of Fluorescence 1
Robert P. Learmonth, Scott H. Kable, and Kenneth P. Ghiggino
1.1 Introduction 1
1.1.1 Properties of Light 2
1.1.2 States of Molecules 5
1.2 Absorption and Emission of Light 9
1.2.1 Interaction of Light with a Molecule 9
1.2.2 Absorption and Emission of Light Depicted
on a Perrin-Jablonski Diagram 10
1.2.3 Multiphoton Excitation 11
1.3 Nonradiative Decay Mechanisms 13
1.3.1 Vibrational Relaxation 13
1.3.2 Internal Conversion 14
1.3.3 Intersystem Crossing 14
1.4 Properties of Excited Molecules 16
1.4.1 Quantum Yield 16
1.4.2 Excited-State Lifetime 16
1.5 Spectroscopy and Fluorophores 16
1.5.1 Absorption, Excitation, and Emission Spectra 16
1.5.2 Basic Properties of Fluorophores 19
1.6 Environmental Sensitivity of Fluorophores 20
1.6.1 Quenching of Fluorescence 21
1.6.2 Photobleaching 21
1.6.3 Fluorescence Resonance Energy Transfer 22
1.6.4 Solvent Relaxation 22
1.7 Polarization of Fluorescence 24
v
Vi CONTENTS
1.8 Conclusion 26
References 26
2 Labeling of Cells with Fluorescent Dyes 27
Ian S. Harper
2.1 Introduction 27
2.2 Fluorophore Selection 32
2.3 Loading and Labeling Live Cells 34
2.3.1 Loading AM Forms 35
2.3.2 Leakage and Hydrolysis of AM Esters 36
2.4 Fluorophores for Live Cell Imaging 36
2.4.1 Cell Viability 36
2.4.2 General Morphology 36
2.4.3 Specific Organelles 37
2.4.4 Ionic Environment 38
2.4.5 Tracers for Membranes and Cells 39
2.4.6 Fluorescent Proteins 40
References 41
3 Genetically Encoded Fluorescent Probes: Some Properties
and Applications in Life Sciences 47
Mark Prescott and Anya Salih
3.1 Introduction 47
3.2 Chromophore and its Formation 49
3.3 Life and Death of Fluorescent Protein 54
3.3.1 Oligomerization 57
3.3.2 Fusion Proteins 58
3.3.3 Photobleaching 58
3.3.4 Destabilization 59
3.4 Applications 60
3.5 Passive Applications 61
3.5.1 Photoamplifying PAFPs 62
3.5.2 Photoconverting PAFPs 63
3.5.3 Kindling PAFPs 64
3.5.4 Fluorescent Timers 64
3.6 Active Applications 65
3.6.1 Protein-Protein Interactions 65
3.6.2 Monitoring Enzyme Activity 66
3.6.3 Monitoring Small Molecules and Metabolites 66
3.6.4 Monitoring pH and Other Small Molecules 67
3.6.5 Monitoring Redox 67
3.7 Interactive Applications 67
CONTENTS VM
3.8 Conclusions 68
References 69
4 Nanoparticle Fluorescence Probes 75
Krystyna Drozdowicz-Tomsia and Ewa M- Goldys
4.1 Introduction 75
4.2 Nanomaterials for Biological Applications 75
4.3 Inorganic Quantum Dots: Physics and Optical Properties 77
4.4 Synthesis of Monodisperse Colloidal Quantum Dots
for Biolabeling Applications 81
4.4.1 Quantum Dot Synthesis 82
4.4.2 Shell Synthesis 84
4.4.3 Water Solubilization and Functionalization 85
4.4.4 Quantum Dot Bioconjugation 88
4.5 Quantum Dots as In Vitro Probes 89
4.6 Quantum Dots as In Vivo Probes 92
4.7 Cytotoxicity 94
4.8 Future Directions 94
References 95
5 Quantitative Analysis of Fluorescent Image: From Descriptive
to Computational Microscopy 99
Michal Marek Godlewski, Agnieszka Turowska, Paulina Jedynak,
Daniel Martinez Puig, and Helena Nevalainen
5.1 Introduction 99
5.2 Advantages of Quantitative Analysis 100
5.3 Methods of Quantitative Analysis 101
5.4 Image Processing 113
References 115
6 Spectral Imaging and Unmixing 117
Pascal Vallotton, Aloke Phatak, and Mark Herman
6.1 Introduction 117
6.2 Instrumentation and Configurations for Hyperspectral
Microscopes 119
6.3 Supervised and Unsupervised Unmixing 121
6.4 Supervised or Informed Unmixing 121
6.5 Unsupervised or Blind Unmixing 122
6.5.1 Iterated Constrained End-Members Algorithm 123
6.6 Spectral Clustering 124
6.7 Examples 125
6.7.1 Tablet Inspection 125
6.7.2 Fluorescent Microspheres 127
Viii CONTENTS
6.7.3 Induced Mouse Lung Tumor 131
6.8 Limitations of Spectral Imaging and Experimental
Considerations 135
6.8.1 Fast Biological Processes 135
6.8.2 Calibration 136
6.8.3 Environmental Sensitivity 137
6.8.4 Background Fluorescence 137
6.9 Conclusions 137
References 138
7 Correlation of Light with Electron Microscopy: A Correlative
Microscopy Platform 141
Marko Nykdnen
7.1 Introduction 141
7.2 Overview of Techniques Used in Correlative Microscopy 142
7.3 Correlative Microscopy of Chemically Fixed, Imtfiunolabeled
Ultrathin Cryosections Employing Antibodies Coupled
with Fluorescent Gold Conjugates 145
7.4 Special Techniques Used in Sample Preparation for Correlative
Microscopy: High-Pressure Freezing, Freeze Fracturing,
and Freeze Substitution 151
7.5 Correlative Microscopy of Fixed Tissue Specimens Using
Freeze Substitution 153
7.6 Future Trends: Correlative Microscopy and High-Content
Cellular Screening 154
7.7 Future Trends: Correlative Microscopy of Fluorescent Images
Acquired by Confocal Laser Scanning Microscopy 154
References 155
8 Fluorescence Resonance Energy Transfer and Applications 157
Andrew Clayton
8.1 What is FRET? 157
8.1.1 Theory of FRET 157
8.2 Why FRET Can Be Useful 159
8.3 How FRET Can Be Measured 160
8.3.1 Applications of FRET in Spectroscopy 161
8.3.2 Applications of FRET in Microscopy 164
8.4 Emerging Applications Including Novel FRET Probes 169
8.4.1 Intramolecular FRET 169
8.4.2 Intermolecular FRET 171
8.5 Advanced FRET Methods 171
References 172
CONTENTS ix
9 Monitoring Molecular Dynamics in Live Cells Using
Fluorescence Photobleaching 175
Nectarios Klonis and Leann Tilley
9.1 Introduction 175
9.2 Photobleaching Theory 176
9.3 Dynamics of Macromolecules 177
9.3.1 Macromolecular Mobilities 177
9.3.2 Fluorescence Recovery After Photobleaching 179
9.4 Photobleaching Measurements with Confocal Microscope 183
9.5 Photobleaching Applications 187
9.5.1 Macromolecular Organization 187
9.5.2 Monitoring Movement between Different Compartments 190
9.5.3 Fluorescence Loss in Photobleaching 191
9.5.4 Fluorescence Resonance Energy Transfer 192
9.6 Conclusions 194
References 194
10 Time-Resolved Fluorescence in Microscopy 195
Trevor A. Smith, Craig N. Lincoln, and Damian K. Bird
10.1 Introduction 195
10.2 Photophysics and Deactivation of Excited State 195
10.3 Time-Resolved Fluorescence Measurements 197
10.3.1 Deviations from Ideal Decay Behavior 198
10.3.2 Time-Resolved Fluorescence Techniques 200
10.4 Time-Resolved Fluorescence in Microscopy 206
10.4.1 Factors That Influence Choice of TRFM Method 213
10.5 Conclusions 218
References 218
11 Fluorescence Correlation Spectroscopy 223
Thomas Dertinger, Iris von der Hocht, Anastasia Loman, Ingo Gregor,
and Jb rg Enderlein
11.1 Introduction 223
11.2 Optics of Fluorescence Correlation Spectroscopy 227
11.3 Practical Aspects of FCS Experiments 229
11.4 Quantitative Evaluation of FCS Measurements to
Obtain Diffusion Constants and Concentration 230
11.4.1 One-Focus FCS 230
11.4.2 Two-Focus FCS 237
11.5 Conclusion 243
References 243
X CONTENTS
12 Flow Cytometry 245
Russell E. Connolly, Graham Vesey, and Charlotte Morgan
12.1 What is Flow Cytometry? 245
12.1.1 History of Flow Cytometry 246
12.1.2 Flow Cytometry Fundamentals 248
12.1.3 Sensitivity of Flow Cytometers 251
12.2 Excitation Sources 252
12.2.1 Laser Excitation 252
12.2.2 Gas Laser Sources 253
12.2.3 Diode Lasers 254
12.2.4 Solid-State Lasers 254
12.3 Signal Detection and Analysis 255
12.3.1 Forward Scatter 255
12.3.2 Side Scatter 255
12.3.3 Fluorescence 256
12.3.4 Issues in Flow Cytometry Using Fluorescence:
Fluorescence Compensation 256
12.3.5 Multiparameter Analysis 258
12.4 Cell Sorting 258
12.5 Applications of Flow Cytometry 260
12.5.1 Calibration 260
12.5.2 Use of Beads for Diagnostic Purposes 261
12.5.3 Water Testing 262
12.5.4 Milk Analysis 262
12.5.5 Brewing and Wine Production 263
12.5.6 Food Microbiology 264
12.5.7 Quality Control in Microbiological Testing in
Various Industries 264
12.5.8 Cytometry: Where to Now? 265
References 266
13 Fluorescence in Diagnostic Imaging 269
Morry Silberstein
13.1 Introduction 269
13.2 Principles of Fluorescence Applied to Medical Diagnosis 269
13.2.1 Contrast Agents 269
13.2.2 Delivery Issues 273
13.2.3 Amplification Strategies 273
13.2.4 Introduction to Imaging Techniques 274
13.3 Optical Fluorescence Imaging Techniques In Vivo 275
13.3.1 Near-Infrared Fluorescence Imaging 275
CONTENTS Xi
13.3.2 Fluorescence Reflective Imaging 275
13.3.3 Fluorescence Molecular Tomography 277
13.3.4 Superficial Confocal Imaging (Optical Coherence
Tomography) 278
13.3.5 Other Techniques 280
13.4 Imaging of Whole-Body Biological Systems 280
13.4.1 Superficial Tumors 280
13.4.2 Subsurface Tumors and Vascularity 281
13.4.3 Lymphoreticular System 282
13.4.4 Bone 283
13.4.5 Brain 283
13.5 Future Directions 283
References 286
14 Fluorescence in Clinical Diagnosis 289
pouter Kalle, Phillip Bwititi, and Todd Walker
14.1 Introduction 289
14.2 Applications of Fluorescence in Clinical Biochemistry 289
14.2.1 Advantages of Fluorescence Measurements 289
14.2.2 Categories of Fluorescence Measurements 290
14.2.3 Applications 291
14.3 Fluorescence in Pathology and Cancer Diagnostics 297
14.3.1 Fluorescent In Situ Hybridization 297
14.3.2 Applications of Fluorescence in Clinical Cytology 301
References 304
15 Imituinochemical Detection of Analytes by Using Fluorescence 309
Evgenia G. Matveeva, Ignacy Gryczynski, Zygmunt Gryczynski, and Ewa M. Goldys
15.1 Introduction: Definition and General Principles of Immunoassays 309
15-2 Immunoassay Types and Formats 311
15.2.1 Competitive and Sandwich Immunoassays 311
15.2.2 Homogeneous and Heterogeneous (Solid-Phase)
Immunoassays 311
15.2.3 Types of Labels Used in Immunoassays with
Fluorescence Detection (Fluorophores, Enzymes
with Fluorogenic Substrates) 313
15-3 Types of Analytes 313
15.3.1 Cations, Metal Ions, and Anions 313
15.3.2 Small-Molecule Analytes (Pesticides, Toxins,
and Biomarkers) 314
15.3.3 Proteins 314
15-4 Steady-State Fluorescence Immunoassays 314
Xii CONTENTS
15.4.1 Intensity-Based (Quenching, Enhancement) Assays:
Environmentally Sensitive Probes 314
15.4.2 Energy Transfer Assays 315
15.4.3 Chemiluminescence Assays: Enzyme Immunoassays
Utilizing Fluorescent Substrates 315
15.4.4 Polarization Assays 317
15.5 Time-Resolved and Kinetic Approaches as Tools for Elimination
of Fluorescence Background 318
15.5.1 Kinetic Approach, Stopped-Flow Immunoassays,
Time-Gated Detection, and Lifetime Assays 318
15.5.2 Near-Infrared Fluorophores 319
15.6 Recent Advances in Immunoassay Signal Enhancement
and Throughput 320
15.6.1 Metal-Particle-Enhanced Immunoassays:
Nanoparticles as Labels 320
15.6.2 Surface Plasmon-Coupled Emission-Based
Immunoassays 321
15.6.3 Assay Miniaturization (Arrays, Microchips) 322
References 322
16 Membrane Organization 327
Astrid Magenau, Carles Rentero, and Katharina Gaus
16.1 Concepts of Organization of Biological Membranes 327
16.1.1 Bilayer 327
16.1.2 Conceptual Models of Membrane Organization 328
16.2 Fluorescence Methods to Study Membrane Organization 330
16.2.1 Fluorescence Intensity Imaging and Membrane Probes 330
16.2.2 Fluorescence Recovery After Photobleaching 332
16.2.3 Fluorescence Correlation Spectroscopy and Image
Correlation Spectroscopy 333
16.2.4 Fluorescence Resonance Energy Transfer and
Fluorescence Lifetime Imaging Microscopy 334
16.2.5 Total Internal Reflection Fluorescence Microscopy 335
16.2.6 Single-Particle Tracking 335
16.3 Model Membranes 336
16.4 Membrane Organization in Cells 340
16.4.1 Lipid Organization in Cell Membrane 340
16.4.2 Protein-Lipid Interactions 341
16.4.3 Protein-Protein Interactions 345
References 347
CONTENTS Xiii
17 Probing Kinetics of Ion Pumps Via Voltage-Sensitive Fluorescent Dyes 349
Ronald J. Clarke
17.1 Introduction: Voltage-Dependent Physiological Processes,
Ion Pumps, and Channels 349
17.2 Voltage-Sensitive Dyes: Mechanisms, Response Times,
and Their Relevance for Kinetic Studies 351
17.3 Steady-State Ion Pump Activity 355
17.4 Kinetics of Ion Pump Partial Reactions 358
17.5 Future Directions 361
References 362
Index 365
|
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| indexdate | 2024-07-09T22:36:39Z |
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| isbn | 0470083700 9780470083703 |
| language | English |
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| physical | XXVII, 367 S. teilw. farb. Ill., graph. Darst. |
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| spelling | Fluorescence applications in biotechnology and life sciences ed. by Ewa M. Goldys Oxford Wiley-Blackwell 2009 XXVII, 367 S. teilw. farb. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biowissenschaften (DE-588)4129772-6 gnd rswk-swf Fluoreszenzanalyse (DE-588)4154821-8 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Fluoreszenzanalyse (DE-588)4154821-8 s Biowissenschaften (DE-588)4129772-6 s DE-604 Biotechnologie (DE-588)4069491-4 s Goldys, Ewa M. edt HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020166991&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fluorescence applications in biotechnology and life sciences Biowissenschaften (DE-588)4129772-6 gnd Fluoreszenzanalyse (DE-588)4154821-8 gnd Biotechnologie (DE-588)4069491-4 gnd |
| subject_GND | (DE-588)4129772-6 (DE-588)4154821-8 (DE-588)4069491-4 |
| title | Fluorescence applications in biotechnology and life sciences |
| title_auth | Fluorescence applications in biotechnology and life sciences |
| title_exact_search | Fluorescence applications in biotechnology and life sciences |
| title_full | Fluorescence applications in biotechnology and life sciences ed. by Ewa M. Goldys |
| title_fullStr | Fluorescence applications in biotechnology and life sciences ed. by Ewa M. Goldys |
| title_full_unstemmed | Fluorescence applications in biotechnology and life sciences ed. by Ewa M. Goldys |
| title_short | Fluorescence applications in biotechnology and life sciences |
| title_sort | fluorescence applications in biotechnology and life sciences |
| topic | Biowissenschaften (DE-588)4129772-6 gnd Fluoreszenzanalyse (DE-588)4154821-8 gnd Biotechnologie (DE-588)4069491-4 gnd |
| topic_facet | Biowissenschaften Fluoreszenzanalyse Biotechnologie |
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