Manual of microbiology: tools & techniques
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Tunbridge Wells
Anshan [u.a.]
2007
|
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVIII, 405 S. Ill., graph. Darst. |
| ISBN: | 1904798985 9781904798989 |
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MARC
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| 264 | 1 | |a Tunbridge Wells |b Anshan [u.a.] |c 2007 | |
| 300 | |a XVIII, 405 S. |b Ill., graph. Darst. | ||
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Record in the Search Index
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|---|---|
| adam_text | CONTENTS
Foreword vii
Preface be
Acknowledgements xi
1. INTRODUCTIONTOMICROBIOLOGY 1-36
Introduction 1
Significance of Microbes 2
Development of Microbiology 3
Birth of Microbiology 3
Spontaneous Generation v/s Germ Theory 3
Landmarks of Microbiology 5
Type of Cells 7
General Characteristics of Bacteria 9
Classification of Microorganisms 23
Constituent Organisms of Maj or Groups of Prokaryotes 32
Domain Archaea 35
2. TOOLSOFMCROBIOLOGY 37-98
Microscopes and Microscopy 37
Principles of Microscopy 39
Light Microscope 43
Electron Microscope (EM) 52
Some New Generation Microscopes 55
Care and use of the Microscope 56
Spectrophotometer 58
pH Meter 62
Micrometer and Micrometry 67
Hemocytometer 71
Autoclave 74
Centrifuge 78
Balance 80
Pipettes 83
Vacuum Filtration 86
Water Bath 87
Biological Safety Cabinets 88
Laminar Air Flow Bench (Laf Bench) 88
xiv Contents
Glassware 90
Inoculation Needle and Loop 93
Hot Air Oven 93
Incubator 94
Colony Counter 96
Lyophilizer (Freeze Dryer) 97
Micro-well Plates 97
3. TECHNIQUES OF MICROBIOLOGY 99-148
Sterilisation 99
Observation of Microorganisms 104
Staining of Microorganisms 109
Simple Staining 112
Negative Staining 112
Differential Staining 113
Micro Chemical Staining 118
Cytological Staining 123
Organism Specific Staining 130
Measurement of Microbial Size 143
Measuring of Microscopic Numbers 144
Sample Collection 146
Titration 147
4. CULTURE OF MICROORGANISMS 149-174
Culture Media 150
Components of Media 151
Role of Substances Added to the Media 151
Types of Media 152
Interaction of Media Components 155
Preparation of Nutrient Medium 155
Culture Techniques 157
5. ISOLATION, PURIFICATION AND IDENTIFICATION OF BACTERIA 175-200
Isolation and Purification of Bacteria 175
Identification of Bacteria 176
Rapid Methods for Identifying Microbes 196
6. MAINTENANCE AND STORAGE OF BACTERIAL STRAINS 201-204
7. LABORATORYEXERCISESTOSTUDYBACTERIA 205-220
Study of Motility by Hanging Drop Method 205
Calibration of Micrometer and Measurement of Microbial Size 206
Simple Staining of Bacteria 207
Negative Staining of Bacteria 207
Differential Staining: Gram Staining 208
Contents xv
Differential Staining: Acid Fast Staining 208
Cytological Staining: Endospore 209
Cytological Staining: Capsule 209
Cytological Staining: Flagella 210
Staining of intracellular Metabolites 210
Culture of Bacteria on Solid Medium 211
Broth Culture of Bacteria 213
Isolation and Development of Pure Cultures of Bacteria 213
Determination of Cell Numbers by Turbidimetric Method 214
Determination of Cell Numbers by Hemacytometer 215
Determination of Viable Count 216
Anaerobic Culture of Bacteria 216
Sampling of Air for Presence of Microbes 217
Culture of Bacteria to Show Ubiquitous Presence of Microorganisms 218
Isolation of Bacteria by Using Differential and Selective Media 219
8. STUDY OF BACTERIAL GROWTH 221-226
Enumeration of Cell Numbers 222
Determination of Viable Count 223
To Prepare the Growth Curve of Bacteria 224
To Compare Growth of Bacteria on Different Media 225
9. STUDY OF EFFECT OF ENVIRONMENT ON BACTERIAL GROWTH 227-238
Factors that Effect Growth 227
Study of Effect of Temperature on Growth 230
Study of Lethal Effect of Temperature on Growth 230
Study of Heat Resistance of Vegetative Cells and Spores 231
Study of Effect of Osmotic Pressure on Growth 232
Study of Effect of Medium pH on Growth 233
Study of Energy Source Preference on Growth 233
Study of Effect of Energy Source and Role of Buffers on Growth 234
Microbiological Assay for Nutrient Requirement 234
Culture of Photosynthetic Bacteria 236
Study of Sequential Dependency of Bacteria by Winogradsky Column 236
Study of Oxygen Requirements of Bacteria 237
10. STUDY OF CONTROL OF MICROBIAL GROWTH BY ANTIMICROBIAL AGENTS 239-246
Educational Objectives 239
Physical Agents of Controls 239
Chemical Agents of Controls 240
Study of Effect of Antiseptic and Disinfectant Action on Bacteria 240
Determination of Phenol Coefficient 241
Study of Lethal Action of UV Light and Photo Reactivation on Bacteria 242
Study of Action of Antimetabolites on Bacteria 244
Isolation of Antibiotic Producing Strain or Species 245
xvi Contents
11. STUDY OF BACTERIAL GENETICS 247-252
Isolation of Temperature Variants 247
Isolation of Antibiotic Resistant Variants 248
Isolation of Nutritional Mutants 249
Isolation of Conjugation Mutants 249
Isolation of Transformation Mutants 251
12. WATERMICROBIOLOGY 253-270
Standard Plate Count (Heterotrophic Plate Count: HPC) 254
Membrane Filtration Technique (MF) 256
Standard Analysis of Water for the Presence of Coliforms 257
Determination of Biochemical Oxygen Demand (BOD) 258
Determination of Total Suspended Solids (TSS) 259
Determination of Dissolved Oxygen (DO) by Azide Modification Method 260
Chemical Oxygen Demand (COD) by Open Reflux Method 260
Determination of Dissolved Organic Matter and Dissolved inorganic Matter (DOM DIM) 261
Identification of Enteric Bacilli by IMViC Test 262
Determination of Most Probable Number (MPN) 263
Screening of Intestinal Bacteria by TSI Agar 265
Selective Culture of E.Coli 267
Enrichment Culture of Pseudomonas Spp. TjSJ
Enrichment Culture of Shigella 268
Enrichment Culture of Proteus 268
Enrichment Culture of Salmonella 269
Enrichment Culture of Vibrio 269
Total Coliform Test 270
13. SOILMCROBIOLOGY 271-280
Isolation and Enumeration of Soil Bacteria 271
Isolation of Ammonifying Bacteria 272
• Isolation of Nitrifying (Nitrite forming) Bacteria 273
Isolation of Nitrifying (Nitrate forming) Bacteria 274
Isolation of Denitrifying Bacteria 274
Isolation of Rhizobia From Root Nodules 275
Differentiation of Rhizhobia 276
Enrichment Culture of Bacillus 277
Selective Culture of Bacillus 278
Detection of Siderophore-production by Soil Bacteria 278
Detection of Psosphate Solubilizing Ability of Soil Bacteria 279
14. FOODMCROBIOLOGY 281-288
Sample Collection and Preparation 281
Direct Microscopic Examination of Foods (Except Eggs) 281
Direct Microscopic Examination of Frozen or Liquid Egg Products 282
Direct Microscopic Examination of Dried Egg Products 282
Contents Xvii
Microbiology of Cheese 283
Preparation of Sauerkraut 284
Preparation of Butter Milk 285
Preparation of Alcohol From Fruit Juices 285
Production of Wine 285
Examination of Food for Presence of Food Poisoning Bacteria 286
15. MUJCMICROBIOLOGY 289-298
Measurement of Colif orms in Milk 289
Determination of Phosphatase Activity (Phosphatase Test) 291
Organoleptic Tests 295
Clot on Boiling (C.O.B) Test 295
The Alcohol Test 295
The Alcohol-alizarin Test 295
Acidity Test 2%
ResazurinTest 296
Methylene Blue Reductase Test 297
16. STUDY OF VIRUSES 299-306
Examples of Some Common Viral Pathogens 301
Isolation of Bacteriophages from Raw Sewage by Plaque Culture 302
Enumeration of Bacteriophages 303
Culture of Viruses in Monolayers 304
Culture of Viruses in Chick Embryo 304
Culture of Plant Viruses 305
Culture of TMV in Root Tips 306
17. STUDYOFFUNGI 307-316
Culture of Fungi in Moist Chamber 309
Isolation, Purification and Identification of Fungi 309
Study of Yeast Morphology 311
Isolation of Yeasts 311
Culture of Yeasts on Selective Medium 314
Culture of Baker s Yeasts 314
Culture of Bakers Yeast on Different Carbon and Energy Sources 315
18. STUDYOFCYANOBACTERIA 317-318
General Characteristics 317
Isolation and Culture of Cyanobacteria 318
19. STUDY OF BACTERIAL DISEASES IN PLANTS 319-322
Stem Streaming Test 319
Ooze Test 319
Detection of Poly-B-hydroxybutyrate Granules 320
xviii Contents
Isolation of the Plant Pathogenic Bacteria 320
Identification of Pure Culture 321
Enrichment Culture of Xanthomonas 322
20. STUDYOFMEDICALMOIOBIOLOGYANDIMMUNOLOGY 323-334
Antibiotic Sensitivity Test 323
Isolation of Micro Flora of Skin 324
Isolation of Oral Micro Flora 325
Study of Sputum or Urine Specimens for Tuberculosis 325
Study of Bacteria in Stool Sample 325
Identification of Staphylococci by Coagulase Test 325
Phage Typing of Coagulase Positive Staphylococcal Strains 326
Identification of Staphylococci by DNAse Test 326
Identification of Gram-positive Streptococci 327
Culture and Identification of Fastidious B acteria 329
Antigen-Antibody Reactions 330
Identification of an Antigen-Antibody Complex by Gel Diffusion Analysis
(Immunodiffusion) or Ouchterlony Plate Method 330
Identification of an Antigen-Antibody Complex by Precipitin Test 331
Identification of an Antigen-Antibody Complex by Slide Agglutination Test 331
Identification of an Antigen-Antibody Complex by Complement Fixation 332
21. GOODLABORATORYPRACnCES 335-342
Rules for Maintaining Aseptic Conditions 336
Rules for Maintaining Safety 339
Rules for Efficient Working 341
Recording Practical Work 342
ANNEXUREI 343
ANNEXUREH 347
ANNEXUREm 357
ANNEXUREIV 361
ANNEXUREV 379
ANNEXUREVI 387
ANNEXUREVn 389
INDEX 393
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T22:36:13Z |
| institution | BVB |
| isbn | 1904798985 9781904798989 |
| language | English |
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| physical | XVIII, 405 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
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| publisher | Anshan [u.a.] |
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| spelling | Sharma, Kanika Verfasser aut Manual of microbiology tools & techniques Kanika Sharma Tunbridge Wells Anshan [u.a.] 2007 XVIII, 405 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020146599&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sharma, Kanika Manual of microbiology tools & techniques |
| title | Manual of microbiology tools & techniques |
| title_auth | Manual of microbiology tools & techniques |
| title_exact_search | Manual of microbiology tools & techniques |
| title_full | Manual of microbiology tools & techniques Kanika Sharma |
| title_fullStr | Manual of microbiology tools & techniques Kanika Sharma |
| title_full_unstemmed | Manual of microbiology tools & techniques Kanika Sharma |
| title_short | Manual of microbiology |
| title_sort | manual of microbiology tools techniques |
| title_sub | tools & techniques |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020146599&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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