Transgenic maize: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2009
|
| Schriftenreihe: | Methods in molecular biology
526 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 180 S., [8] Taf. Ill. |
| ISBN: | 9781934115497 |
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Datensatz im Suchindex
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| adam_text | Titel: Transgenic maize
Autor: Scott, M. Paul
Jahr: 2009
Contents
Preface............................................................ v
Contributors........................................................ ix
Part I: Introduction
1 Design of Gene Constructs for Transgenic Maize........................ 3
Dong Liu
Part II: Transformation Methods
2 Transient Expression of GFP in Immature Seed Tissues................... 23
Colin T. Shepherd
3 Biolistic Gun-Mediated Maize Genetic Transformation.................... 29
Kan Wang and Bronwyn Frame
4 Agrobacterhtm-M.ediated Maize Transformation:
Immature Embryos Versus Callus.................................... 47
Vladimir Sidorov and David Duncan
5 Whiskers-Mediated Maize Transformation............................. 59
Joseph F. Petolino and Nicole L. Arnold
Part III: Transgenic Maize in Research
6 Methods for Generation and Analysis of Fluorescent
Protein-Tagged Maize Lines....................................... 71
Amitabh Mohanty, Tan Tang, Anding Luo, Anne Sylvester,
and David Jackson
7 Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression ... 91
Karen McGinnis
8 Plasmid Rescue: Recovery of Flanking Genomic Sequences
from Transgenic Transposon Insertion Sites............................ 101
Guo-Ling Nan and Virginia Walbot
Part IV: Analysis of Transgenic Plants
9 Nonradioactive Genomic DNA Blots for Detection of Low-
Abundant Sequences in Transgenic Maize............................. 113
Guo-Ling Nan and Virginia Walbot
10 Tissue-Print Immunodetection of Transgene Products
in Endosperm for High-Throughput Screening of Seeds.................. 123
M. Paul Scott
11 Determination of Transgene Copy Number by Real-Time Quantitative PCR . . . 129
Colin T. Shepherd, Adrienne N. Moran Lauter, and M. Paul Scott
VII
viii Contents
Part V: Breeding with Transgenes
12 Herbicide Resistance Screening Assay................................. 137
Joan M. Peterson
13 Characterizing Transgene Inheritance................................. 147
Earl H. Bicar
14 Backcross Breeding.............................................. 161
Karla E. Vogel
Appendix A........................................................ 171
Appendix B........................................................ 175
Index............................................................. 179
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| isbn | 9781934115497 |
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| spelling | Transgenic maize methods and protocols ed. by M. Paul Scott New York, NY Humana Press 2009 IX, 180 S., [8] Taf. Ill. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 526 Springer protocols Scott, M. Paul edt Methods in molecular biology 526 (DE-604)BV035362695 526 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020145649&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Transgenic maize methods and protocols Methods in molecular biology |
| title | Transgenic maize methods and protocols |
| title_auth | Transgenic maize methods and protocols |
| title_exact_search | Transgenic maize methods and protocols |
| title_full | Transgenic maize methods and protocols ed. by M. Paul Scott |
| title_fullStr | Transgenic maize methods and protocols ed. by M. Paul Scott |
| title_full_unstemmed | Transgenic maize methods and protocols ed. by M. Paul Scott |
| title_short | Transgenic maize |
| title_sort | transgenic maize methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020145649&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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