Holdings: In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.)

In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.):

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Bibliographic Details
Main Author:	Pietsch, Constanze (Author)
Format:	Thesis Book
Language:	English
Published:	Berlin Dissertation de. - Verl. im Internet 2008
Series:	[Dissertationen] 1488
Subjects:	carp immune response Hochschulschrift
Online Access:	Inhaltsverzeichnis
Physical Description:	XVIII, 138 S. Ill., graph. Darst.
ISBN:	9783866243880

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adam_text	TABLEOF CONTENTS LIST OF FIGURES I LISTOFTABLES V LIST OF ACRONYMS AND ABBREVIATIONS VIII SUMMARY XI ZUSAMMENFASSUNG XV 1 INTRODUCTION 1 THE PISCINE IMMUNE SYSTEM 1 ROLEOFTHEARGININEMETABOLISM 3 1.2.1 REGULATION AND FUENCTIONS OF NO 5 1.2.2 REGULATION AND FUENCTIONS OFARGINASES 11 STRESS AND THE IMMUNE RESPONSE IN FISH 13 THE INFLUENCE OF PROGESTOGENS ON NO PRODUCTION 15 OBJECTIVES OF THE STUDY 20 2 MATERIAL AND METHODS 21 I IN VITRO STUDIES 2.1 HUSBANDRY OF EXPERIMENTAL ANIMALS 21 2.2 CHEMICALS AND REAGENTS FOR CELL CULTURE 21 2.3 CELL ISOLATION AND CULTURE OFLEUKOCYTES 22 2.3.1 CHEMICALS USED FOR INCUBATION TO DETERMINE EFFECTS OF SECONDARY MESSENGERS (CGMP, CAMP, CALCIUM) ON NO REGULATION 23 2.3.2 PROGESTOGEN AND GLUCOCORTICOID EFFECTS ON NO PRODUCTION 24 2.3.3 CHEMICALS USED TO INVESTIGATE THE INFLUENCE OF G PROTEIN FUNCTION ON NO PRODUCTION 24 2.4 PREPARATION OF BACTERIAL SUSPENSIONS FOR LEUKOCYTE STIMULATION 25 2.5 MEASUREMENTOFREACTIVENITROGEN PRODUCTION 26 2.6 MEASUREMENTOFCELL VIABILITY (MTT ASSAY) 27 2.7 MEASUREMENT OF EARLY APOPTOTIC EVENTS 28 2.8 RNA EXTRACTION AND SEMI-QUANTITATIVE RT-PCR 29 BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/990812049 DIGITALISIERT DURCH _TA BLE_OF_CONTENTS_ II IN VIVO TREATMENT WITH STEROIDAL COMPOUNDS 2.9 HUSBANDRY 33 2.10 EXPERIMENTAL DESIGN 33 2.11 ARGINASE ASSAY 35 2.12 NBT ASSAY 37 2.13 ANALYSES OFPLASMA PARAMETERS 38 2.13.1 ANALYSES OFPLASMA CORTISOL LEVELS 38 2.13.2 ANALYSES OF TOTAL PLASMA PROTEIN 39 2.14 CALCULATION AND STATISTICS 40 3 RESULTS 41 I REGULATION OF NO PRODUCTION IN VITRO 3.1 IN VITRO STIMULATION 41 3.2 CGMP-DEPENDENT NO SIGNALING IN CARP LEUKOCYTES 42 3.3 REGULATION OF NO PRODUCTION BY INTRACELLULAR CAMP 46 3.4 EFFECTS OF INCREASED INTRACELLULAR CALCIUM CONCENTRATIONS ON NO PRODUCTION 49 3.5 EFFECTS OFGLUCOCORTICOIDSON NO PRODUCTION BYKIDNEY LEUKOCYTES 52 3.6 INFLUENCE OFPROGESTOGENS ON NO PRODUCTION OF CARP LEUKOCYTES 52 3.7 PARTICIPATIONOFGPROTEINS IN NO SIGNALING 59 3.8 POSSIBLE SPECIFIC BINDING SITES FOR PROGESTOGENS IN LEUKOCYTES 61 II EX VIVO CHALLENGE WITH AEROMONAS HYDROPHILA AFTER HORMONE TREATMENT 3.9 HUSBANDRY 63 3.10 BODY CHARACTERISTICS OF FISH 63 3.11 EFFECTS OF STEROIDAL TREATMENT ON PLASMA CORTISOL LEVELS 65 3.12 EFFECTS OF STEROIDAL COMPOUNDS ON TOTAL PROTEIN LEVELS IN THE PLASMA 66 3.1 PUBLICATIONS 137 _TABLE_OF_CONTENTS_ 4 DISCUSSION 79 I REGULATION OF NO PRODUCTION IN VITRO 4.1 IMPACT OF SECONDARY MESSENGERS ON NO PRODUCTION 79 4.2 EFFECTS OF GLUCOCORTICOIDS ON NO PRODUCTION IN VITRO 88 4.3 INFLUENCE OF PROGESTOGENS ON NO PRODUCTION IN VITRO 89 4.4 POSSIBLE BINDING SITES FOR PROGESTOGENS 93 4.5 PARTICIPATIONOF G PROTEINS IN NO SIGNALING 95 II IN VIVO EXPERIMENTS WITH STEROIDAL COMPOUNDS 4.6 CHANGES IN BLOOD CORTISOL CONTENT DUE TO INJECTION OF CARPS WITH CORTISOL 98 4.7 EFFECTS OF CORTISOL ON MRNA EXPRESSION OF SEVERAL PLASMA PROTEINS IN LIVER TISSUE 98 4.8 MORPHOLOGICAL INDICATION OF EFFECTS OF CORTISOL INJECTION ON IMMUNE PARAMETERS 101 4.9 ARGININE METABOLISM IN CORTISOL-TREATED FISH 101 4.10 EFFECTS OF STEROIDAL COMPOUNDS ON ROS PRODUCTION BY LEUKOCYTES 104 4.11 EFFECTS OF PROGESTOGENS ON PLASMA CORTISOL CONCENTRATIONS 105 4.12 EFFECTS OF PROGESTOGENS ON PLASMA PROTEINS 105 4.13 EFFECTS OF PROGESTOGENS ON IMMUNE FUNCTION 108 4.14 ARGININE METABOLISM IN CARPS INJECTED WITH PROGESTOGENS 109 5 GENERAL CONCLUSION 111 6 REFERENCES 115 7 ACKNOWLEDGEMENTS 133 ERKLAERUNG 135
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isbn	9783866243880
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physical	XVIII, 138 S. Ill., graph. Darst.
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spelling	Pietsch, Constanze Verfasser aut In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.) von Constanze Pietsch Berlin Dissertation de. - Verl. im Internet 2008 XVIII, 138 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier [Dissertationen] 1488 Zugl.: Berlin, Humboldt-Univ., Diss., 2008 carp cabt immune response cabt (DE-588)4113937-9 Hochschulschrift gnd-content [Dissertationen] 1488 (DE-604)BV025253339 1488 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020132481&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Pietsch, Constanze In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.) [Dissertationen] carp cabt immune response cabt
subject_GND	(DE-588)4113937-9
title	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.)
title_auth	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.)
title_exact_search	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.)
title_full	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.) von Constanze Pietsch
title_fullStr	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.) von Constanze Pietsch
title_full_unstemmed	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.) von Constanze Pietsch
title_short	In vitro and in vivo regulation of non-specific immune responses in carp (Cyprinus carpio L.)
title_sort	in vitro and in vivo regulation of non specific immune responses in carp cyprinus carpio l
topic	carp cabt immune response cabt
topic_facet	carp immune response Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020132481&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV025253339
work_keys_str_mv	AT pietschconstanze invitroandinvivoregulationofnonspecificimmuneresponsesincarpcyprinuscarpiol

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