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Gene Cloning: the mechanics of DNA manipulation

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Bibliographic Details
Main Author:	Glover, David M. (Author)
Format:	Book
Language:	Undetermined
Published:	London <<[u.a.]>> Chapman and Hall 1984
Edition:	Rev. ed. [d. Ausg.]: Genetic engineering, cloning DNA. 2. ed.
Series:	Outline studies in biology
Subjects:	Genklonierung Klonierung Rekombinante DNS
Online Access:	Inhaltsverzeichnis
Physical Description:	VIII, 222 S. Ill., graph. Darst.
ISBN:	0412254301 0412266008

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MARC


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Record in the Search Index

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adam_text	IMAGE 1 CONTENTS PREFACE VII 1 THE PRINCIPLES OF CLONING DNA 1 1.1 THE DEBATE ON THE SAFETY OF WORK WITHIN RECOMBI- NANT DNA 2 1.2 GENERAL PRINCIPLES OF CLONING 4 1.3 STRATEGIES FOR GENE CLONING 8 1.4 FUNCTIONAL STUDIES WITH CLONED GENES 18 REFERENCES 19 2 RECOMBINATION AND MUTAGENESIS OF DNA IN VITRO 2 1 2.1 THE ENZYMOLOGY OF IN VITRO DNA RECOMBINATION 21 2.2 THE SYNTHESIS OF DNA FOR CLONING 30 2.3 IN VITRO MUTAGENESIS 37 REFERENCES 45 3 BACTERIOPHAGE VECTORS 48 3.1 BACTERIOPHAGE A 48 3.2 SINGLE-STRANDED DNA PHAGES 72 REFERENCES 81 4 BACTERIAL PLASM ID VECTORS 85 4.1 VECTORS FOR E. COLI 86 4.2 VECTORS FOR OTHER GRAM NEGATIVE ORGANISMS 97 4.3 VECTORS FOR GRAM POSITIVE ORGANISMS 102 REFERENCES 106 IMAGE 2 VI CONTENTS 5 EXPRESSION OF CLONED DN AS IN E. COLI PLASMIDS 110 5.1 PROMOTERS 110 5.2 FUSION PROTEINS - A MEANS TO INCREASE THE STABILITY OF FOREIGN PEPTIDES IN E. COLI 115 5.3 SECRETION OF PROTEINS FROM E. COLI 120 5.4 FACTORS AFFECTING THE TRANSLATION OF HYBRID GENES 121 5.5 POST-TRANSLATIONAL MODIFICATION 125 REFERENCES 126 6 THE PHYSICAL CHARACTERIZATION OF CLONED DNA SEGMENTS AND THEIR COUNTERPARTS WITHIN CHROMOSOMES 128 6.1 PHYSICAL CHARACTERIZATION OF CLONED DNA 129 6.2 DETECTING SPECIFIC NUCLEIC ACID SEQUENCES WITHIN HETEROGENEOUS POPULATIONS OF MOLECULES - GEL TRANS- FER HYBRIDIZATION 141 6.3 MAPPING CLONED DNA TO CHROMOSOMAL LOCI 146 6.4 GENETIC DISEASES 150 REFERENCES 155 7 GENE CLONING IN FUNGI AND PLANTS 158 7.1 SACCHAROMYCES CEREVISIAE 158 7.2 FILAMENTOUS FUNGI 167 7.3 CLONING DNA IN PLANT CELLS - TI PLASMID 168 REFERENCES 175 8 EXPRESSION OF CLONED GENES IN ANIMAL CELLS 179 8.1 DIRECT TRANSFORMATION OF MAMMALIAN CELLS 179 8.2 SV40 VECTORS 182 8.3 ADENOVIRUS VECTORS 193 8.4 BOVINE PAPILLOMA VIRUS VECTORS 197 8.5 RETROVIRAL VECTORS 198 8.6 VACCINIA VIRUS VECTORS 203 8.7 MAXIMIZING EXPRESSION 206 8.8 WHOLE ANIMAL TRANSFORMATION 208 REFERENCES 213 INDEX 219
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author	Glover, David M.
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illustrated	Illustrated
indexdate	2024-07-09T22:01:43Z
institution	BVB
isbn	0412254301 0412266008
language	Undetermined
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physical	VIII, 222 S. Ill., graph. Darst.
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publisher	Chapman and Hall
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series2	Outline studies in biology
spelling	Glover, David M. Verfasser aut Gene Cloning the mechanics of DNA manipulation David M. Glover Rev. ed. [d. Ausg.]: Genetic engineering, cloning DNA. 2. ed. London <<[u.a.]>> Chapman and Hall 1984 VIII, 222 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Outline studies in biology Genklonierung (DE-588)4123275-6 gnd rswk-swf Klonierung (DE-588)4192480-0 gnd rswk-swf Rekombinante DNS (DE-588)4225578-8 gnd rswk-swf Klonierung (DE-588)4192480-0 s Rekombinante DNS (DE-588)4225578-8 s DE-604 Genklonierung (DE-588)4123275-6 s SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018516143&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Glover, David M. Gene Cloning the mechanics of DNA manipulation Genklonierung (DE-588)4123275-6 gnd Klonierung (DE-588)4192480-0 gnd Rekombinante DNS (DE-588)4225578-8 gnd
subject_GND	(DE-588)4123275-6 (DE-588)4192480-0 (DE-588)4225578-8
title	Gene Cloning the mechanics of DNA manipulation
title_auth	Gene Cloning the mechanics of DNA manipulation
title_exact_search	Gene Cloning the mechanics of DNA manipulation
title_full	Gene Cloning the mechanics of DNA manipulation David M. Glover
title_fullStr	Gene Cloning the mechanics of DNA manipulation David M. Glover
title_full_unstemmed	Gene Cloning the mechanics of DNA manipulation David M. Glover
title_short	Gene Cloning
title_sort	gene cloning the mechanics of dna manipulation
title_sub	the mechanics of DNA manipulation
topic	Genklonierung (DE-588)4123275-6 gnd Klonierung (DE-588)4192480-0 gnd Rekombinante DNS (DE-588)4225578-8 gnd
topic_facet	Genklonierung Klonierung Rekombinante DNS
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