Cartilage and osteoarthritis: 1 Cellular and molecular tools
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in molecular medicine
100 Methods in molecular medicine ... |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 358 S. Ill., graph. Darst. |
| ISBN: | 1588292479 |
Internformat
MARC
| LEADER | 00000nam a2200000 cc4500 | ||
|---|---|---|---|
| 001 | BV024159924 | ||
| 003 | DE-604 | ||
| 005 | 20090910 | ||
| 007 | t | ||
| 008 | 090924s2004 ad|| |||| 00||| eng d | ||
| 020 | |a 1588292479 |9 1-58829-247-9 | ||
| 035 | |a (OCoLC)608324362 | ||
| 035 | |a (DE-599)BVBBV024159924 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-83 | ||
| 245 | 1 | 0 | |a Cartilage and osteoarthritis |n 1 |p Cellular and molecular tools |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2004 | |
| 300 | |a 358 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Methods in molecular medicine |v 100 | |
| 490 | 0 | |a Methods in molecular medicine |v ... | |
| 773 | 0 | 8 | |w (DE-604)BV024131241 |g 1 |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018283437&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-018283437 | ||
Datensatz im Suchindex
| _version_ | 1804140249796837376 |
|---|---|
| adam_text | Contents of Volume 1
Cellular and Molecular Tools
Preface v
Contents of Volume 2 ix
Contributors xi
1 Culture and Phenotyping of Chondrocytes in Primary Culture
Sylvie Thirion and Francis Berenbaum 1
2 Culture of Chondrocytes in Alginate Beads
Frederic De Ceuninck, Christophe Lesur, Philippe Pastoureau,
Audrey Caliez, and Massimo Sabatini 15
3 Immortalization of Human Articular Chondrocytes
for Generation of Stable, Differentiated Cell Lines
Mary B. Goldring 23
4 Culture of Immortalized Chondrocytes and Their Use As Models
of Chondrocyte Function
Mary B. Goldring 37
5 Generation of Pluripotent Stem Cells and Their Differentiation
to the Chondrocytic Phenotype
Luis A. Solchaga, lean F. Welter, Donald P. Lennon,
and Arnold I. Caplan 53
6 Semiquantitative Analysis of Gene Expression in Cultured
Chondrocytes by RT-PCR
Gaelle Rolland-Valognes 69
7 Quantification of mRNA Expression Levels
in Articular Chondrocytes With PCR Technologies
Audrey McAlinden, fochen Haag, Brigitte Bau,
Pia M. Gebhard, and Thomas Aigner 79
8 RNA Extraction From Cartilage
Frederic Mallein-Gerin and Jerome Gouttenoire 101
9 Gene Expression Analysis in Cartilage by In Situ Hybridization
Frederic Mallein-Gerin and Jerome Gouttenoire 105
10 Analysis of Differential Gene Expression in Healthy
and Osteoarthritic Cartilage and Isolated Chondrocytes
by Microarray Analysis
Thomas Aigner, Joachim Saas, Alexander Zien, Ralf Zimmer,
Pia M. Gebhard, and Thomas Knorr 109
11 High-Efficiency Nonviral Transfection of Primary Chondrocytes
Jean F. Welter, Luis A. Solchaga, and Matthew C. Stewart 129
vii
viii Contents of Volume 1
12 In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts
by Adenoviral Vectors
Jean-Noel Gouze, Martin J. Stoddart, Elvire Gouze,
Glyn D. Palmer, Steven C. Ghivizzani, Alan J. Grodzinsky,
and Christopher H. Evans 147
13 Changes of Chondrocyte Metabolism In Vitro:
An Approach by Proteomic Analysis
Anne-Marie Freyria and Michel Becchi 165
14 Analysis of Chondrocyte Functional Markers
and Pericellular Matrix Components by Flow Cytometry
Gust Verbruggen, Jun Wang, Lai Wang, Dirk Elewaut,
and Eric M. Veys 183
15 A Simple and Reliable Assay of Proteoglycan Synthesis
by Cultured Chondrocytes
Frederic De Ceuninck and Audrey Caliez 209
16 Assays of Proteoglycan and Collagen Degradation
in Cultures of Rabbit Cartilage Explants
Christophe Lesur and Massimo Sabatini 219
1 7 Production of Antibodies Against Degradative Neoepitopes
in Aggrecan
John S. Mort and Peter J. Roughley 237
18 Immunoassays for Collagens in Chondrocyte
and Cartilage Explant Cultures
R. Clark Billinghurst, Fackson Mwale, Anthony Hollander,
Mirela lonescu, and A. Robin Poole 251
19 Detection of Apoptosis in Cartilage In Situ and in Isolated
Chondrocytes
Darryl D. D Lima, Klaus Kuhn, and Martin K. Lotz 275
20 Expression, Activity, and Regulation of MAP Kinases in Cultured
Chondrocytes
Jang-Soo Chun 291
21 Mechanical Loading of Chondrocytes Embedded in 3D Constructs:
In Vitro Methods for Assessment of Morphological and Metabolic
Response to Compressive Strain
David A. Lee and Martin M. Knight 307
22 In Vitro Physical Stimulation of Tissue-Engineered
and Native Cartilage
Kelvin W. Li, Travis J. Klein, Kanika Chawla, Gayle E. Nugent,
Won C. Bae, and Robert L. Sah 325
Index 353
Contents of Companion Volume
Volume 2: Structure and In Vivo Analysis
1 Generation and Use of Transgenic Mice As Models of Osteoarthritis
Anna-Marja Saamanen and Eero Vuorio
2 Development and Clinical Application in Arthritis of a New
Immunoassay for Serum Type HA Procollagen NH2 Propeptide
Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas,
and Patrick Garnero
3 Histological and Immunohistological Studies on Cartilage
James Melrose, Susan Smith, and Peter Ghosh
4 Histochemical Visualization of the Cartilage Hyaladherins Using
a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe
James Melrose and Susan Smith
5 Methods for Cartilage and Subchondral Bone Histomorphometry
Philippe Pastoureau and Agnes Chomel
6 Laser-Mediated Microdissection As a Tool for Molecular Analysis
in Arthritis
Martin Judex, Elena Neumann, Steffen Gay, and Ulf Muller-Ladner
7 Analysis of Protein Distribution in Cartilage Using
Immunofluorescence and Laser Confocal Scanning Microscopy
Stephan Soeder, Alexander Kuhlmann, and Thomas Aigner
8 Molecular and Biochemical Assays of Cartilage Components
Caroline D. Hoemann
9 Mechanical Characterization of Native
and Tissue-Engineered Cartilage
Albert C. Chen, Stephen M. Klisch, Won C. Bae,
Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz,
Barbara L. Schumacher, and Robert L. Sah
10 Noninvasive Study of Human Cartilage Structure by MRI
Felix Eckstein
11 High-Resolution MRI Techniques for Studies in Small-Animal Models
Olivier Beuf
12 High-Resolution Imaging of Osteoarthritis Using Microcomputed
Tomography
Lydia Wachsmuth and Klaus Engelke
ix
Contents of Volume 2
13 High-Resolution Ultrasonography for Analysis
of Age- and Disease-Related Cartilage Changes
Amena Sa i ed and Pascal Laugier
14 Evaluation of Cartilage Composition and Degradation
by High-Resolution Magic-Angle Spinning Nuclear
Magnetic Resonance
Jiirgen Schiller, Daniel Huster, Beate Fuchs, Lama Naji,
forn Kaufmann, and Klaus Arnold
15 Pulsed-Field Gradient-Nuclear Magnetic Resonance
(PFG NMR) to Measure the Diffusion of Ions and Polymers
in Cartilage: Applications in Joint Diseases
Jiirgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa,
Robert Knauss, and Klaus Arnold
16 Dynamics of Collagen in Articular Cartilage Studied
by Solid-State NMR Methods
Daniel Huster, jiirgen Schiller, and Klaus Arnold
17 Computerized Protocol for Anatomical and Functional Studies
of Joints
Sandra Martelli and Stefano Zaffagnini
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV024159924 |
| ctrlnum | (OCoLC)608324362 (DE-599)BVBBV024159924 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01055nam a2200289 cc4500</leader><controlfield tag="001">BV024159924</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20090910 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">090924s2004 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1588292479</subfield><subfield code="9">1-58829-247-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)608324362</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV024159924</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-83</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cartilage and osteoarthritis</subfield><subfield code="n">1</subfield><subfield code="p">Cellular and molecular tools</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2004</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">358 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in molecular medicine</subfield><subfield code="v">100</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in molecular medicine</subfield><subfield code="v">...</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="w">(DE-604)BV024131241</subfield><subfield code="g">1</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018283437&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-018283437</subfield></datafield></record></collection> |
| id | DE-604.BV024159924 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:56:59Z |
| institution | BVB |
| isbn | 1588292479 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018283437 |
| oclc_num | 608324362 |
| open_access_boolean | |
| owner | DE-83 |
| owner_facet | DE-83 |
| physical | 358 S. Ill., graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in molecular medicine |
| spelling | Cartilage and osteoarthritis 1 Cellular and molecular tools Totowa, NJ Humana Press 2004 358 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 100 Methods in molecular medicine ... (DE-604)BV024131241 1 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018283437&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cartilage and osteoarthritis |
| title | Cartilage and osteoarthritis |
| title_auth | Cartilage and osteoarthritis |
| title_exact_search | Cartilage and osteoarthritis |
| title_full | Cartilage and osteoarthritis 1 Cellular and molecular tools |
| title_fullStr | Cartilage and osteoarthritis 1 Cellular and molecular tools |
| title_full_unstemmed | Cartilage and osteoarthritis 1 Cellular and molecular tools |
| title_short | Cartilage and osteoarthritis |
| title_sort | cartilage and osteoarthritis cellular and molecular tools |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018283437&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV024131241 |