Understanding bioanalytical chemistry: principles and applications
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| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Chichester
Wiley-Blackwell
2009
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Physical Description: | X, 290 S. Ill., graph. Darst. |
| ISBN: | 9780470029060 9780470029077 |
Staff View
MARC
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface
ix
1
Introduction
to biomolecules
1
1.1
Overview of chemical and physical attributes of
biomolecules
2
1.2
Classification of biomolecules
5
1.3
Features and characteristics of major biomolecules
б
1.4
Structure-function relationships
21
1.5
Significance of biomolecules in nature and science
21
2
Analysis and quantification of biomolecules
29
2.1
Importance of accurate determination of biomolecules
30
2.2
Major methods to detect and quantify biomolecules
33
2.3
Understanding mass, weight, volume and density
34
2.4
Understanding moles and molarity
38
2.5
Understanding solubility and dilutions
46
3
Transition metals in health and disease
53
3.1
Structure and characteristics of key transition metals
54
3.2
Importance of transition metals in physiological processes
60
3.3
Transition metals as mediators of disease processes
64
3.4
Therapeutic implications of transition metals
71
3.5
Determination of transition metals in nature
73
4
Ions, electrodes and biosensors
77
4.1
Impact of ions and oxidation-reduction reactions
on physical and life processes
78
4.2 pH,
biochemical buffers and physiological regulation
83
4.3
Chemical and physical sensors and biosensors
88
4.4
Important measurements using specific electrodes
91
4.5
Specific applications of biosensors in life and health sciences
93
5
Applications of spectroscopy
99
5.1
An introduction to
spectroscopie
techniques
100
5.2
Major types of spectroscopy
104
vi
CONTENTS
5.3
Principles and applications of ultraviolet/visible
spectrophotometry
105
5.4
Principles and applications of infrared spectroscopy
113
5.5
Principles and applications of fluorescence spectrofluorimetry
118
6
Centrifugation and separation
123
6.1
Importance of separation methods to isolate biomolecules
124
6.2
Basic principles underlying centrifugation
126
6.3
Features and components of major types of centrifuge
129
6.4
Major centrifugation methods for
bioanalysis
133
6.5
Flow cytometry: principles and applications of this core
method of separation
136
7
Chromatography of biomolecules
141
7.1
Chromatography: a key method for separation and
identification of biomolecules
142
7.2
Principles, types and modes of chromatography
143
7.3
Applications of chromatography in life and health sciences
153
7.4
High-performance liquid chromatography and advanced
separation technologies
154
7.5
Additional state-of-the-art chromatography techniques
160
8
Principles and applications of electrophoresis
163
8.1
Principles and theory of electrophoretic separation
164
8.2
Major types of electrophoresis
165
8.3
Electrophoresis in practice
169
8.4
Applications of electrophoresis in life and health sciences
177
8.5
Advanced electrophoretic separation methodologies
for genomics and proteomics
178
9
Applications of mass spectrometry
183
9.1
Major types of mass spectrometry
184
9.2
Understanding the core principles of mass spectrometry
186
9.3
Major types of mass spectrometry in practice
191
9.4
Mass spectrometry: a key tool for
bioanalysis
in life
and health sciences
194
9.5
Mass spectrometry: future perspectives
196
10
Immunochemical techniques and biological tracers
199
10.1
Antibodies: the keys to immunochemical measurements
200
10.2
Analytical applications of biological tracers
208
10.3
Principles and applications of radioimmunoassay
(RIA)
212
CONTENTS
vii
10.4
Principles and applications of enzyme-linked
immunosorbent assay
(ELISA)
216
10.5
Immunohistochemistry: an important diagnostic tool
221
11
Bioanalysis
by magnetic resonance technologies:
NMR and
MRI
225
11.1
Nuclear magnetic resonance (NMR) and magnetic resonance
imaging
(MRI)
technologies: key tools for the life
and health sciences
226
11.2
Principles of NMR and the importance of this biomolecular
analytical technique
229
11.3
Established and emerging applications of NMR
235
11.4
Principles and uses of
MRI
236
11.5
MRI
as a principal diagnostic and research tool
241
12
Bioanalytical approaches from diagnostic, research
and pharmaceutical perspectives
247
12.1
Clinical genomics, proteomics and metabolomics
248
12.2
Clinical diagnosis and screening
251
12.3
Research and development
254
12.4
Emerging pharmaceutical products
· 258
12.5
Future perspectives
260
13
Self-Assessment
265
Appendix
1:
International system of units (SI) and common
prefixes
273
Appendix
2:
The periodic table of the elements
275
Appendix
3:
Common solvents and biological buffers
277
Appendix
4:
Answers to self-assessment questions
279
Index
281
Understanding
Bioanalytical Chemistry
Principles and Applications
Victor A. Gault and Neville H. McClenaghan
School of
Biomedical
Sciences, University of Ulster, Northern Ireland, UK
Although chemistry is core to the life and health sciences, it is often viewed as a challenging subject.
Conventional textbooks tend to present chemistry in a way that is not always easily accessible to students,
particularly those coming from diverse educational backgrounds, who may not have formally studied
chemistry before.
This prompted the authors to write this particular textbook, taking a new, fresh and innovative approach
to teaching and learning of chemistry, focussing on
bioanalysis
to set knowledge in context. This textbook
is primarily targeted to undergraduate life and health science students, but may be a useful resource for
practising scientists in a range of disciplines.
In this textbook the authors have covered basic principles, terminology and core technologies, which
include key modern experimental techniques and equipment used to analyse important biomolecules in
diagnostic, industrial and research settings.
Written by two authors with a wealth of experience in teaching, research and academic enterprise, this
textbook represents an invaluable tool for students and instructors across the diverse range of biological
and health science courses.
О
Innovative, stand alone teaching and learning resource to enhance delivery of
undergraduate chemistry provision to life and health scientists.
О
Develops student knowledge and understanding of core concepts with reference to relevant
real-life, examples.
О
Clearly written and user-friendly, with numerous full colour illustrations, annotated images,
diagrams and tables to enhance learning.
О
Incorporates a modem approach to teaching and learning to motivate the reader and
encourage student-centred learning.
|
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| spelling | Gault, Victor A. Verfasser aut Understanding bioanalytical chemistry principles and applications Victor A. Gault and Neville H. McClenaghan Chichester Wiley-Blackwell 2009 X, 290 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Analytical biochemistry Textbooks Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 s DE-604 McClenaghan, Neville H. Verfasser aut Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017297851&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017297851&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
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| subject_GND | (DE-588)4255721-5 |
| title | Understanding bioanalytical chemistry principles and applications |
| title_auth | Understanding bioanalytical chemistry principles and applications |
| title_exact_search | Understanding bioanalytical chemistry principles and applications |
| title_full | Understanding bioanalytical chemistry principles and applications Victor A. Gault and Neville H. McClenaghan |
| title_fullStr | Understanding bioanalytical chemistry principles and applications Victor A. Gault and Neville H. McClenaghan |
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| title_short | Understanding bioanalytical chemistry |
| title_sort | understanding bioanalytical chemistry principles and applications |
| title_sub | principles and applications |
| topic | Analytical biochemistry Textbooks Biochemische Analyse (DE-588)4255721-5 gnd |
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