Stem cell culture:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier Acad. Press
2008
|
| Series: | Methods in cell biology
86 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIX, 386 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780123738769 |
Staff View
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Record in the Search Index
| _version_ | 1804137901346258944 |
|---|---|
| adam_text | CONTENTS
Contributors
xi
Preface
xv
Biography
xix
1.
Derivation of Human Embryonic Stem Cells in Standard
and Chemically Defined Conditions
Eric Cimo,
Muriel
Kmet,
Barry Belir, and Julie Baker
I. Introduction
1
II. Methods
2
III. Discussion
12
IV. Materials
12
References
13
2.
Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic
Stem Cells in Feeder and Feeder-Free Conditions
Andre Choo,
Ang
Sheii Ngo, Vanessa Ding, Steve Oh, and
Lim
Sai
Kimig
I. Introduction
16
II. Methods and Materials
17
III. Results
21
IV. Discussion and Summary
25
References
28
3.
Microporous Membrane Growth Substrates for Embryonic Stem Cell
Culture and Differentiation
Steven D. Sheridan,
Sonia
Gii,
Matthew
Wilgo,
and
Aldo Pitt
I. Introduction
30
II. Rationale
32
HI. Methods
32
IV. Summary
54
References
55
Contents
4.
Culture
of
Rodent
Spermatogonial Stem Cells, Male Germline Stem Cells
of the Postnatal Animal
Hiroshi Kubota and Ralph L. Brinster
I. Introduction
60
II. Rationale
63
III. Methods
67
IV. Materials
78
V. Discussion
80
References
81
5.
Characterization of Human
Amniotic
Fluid Stem Cells
and Their Pluripotential Capability
Laura Venn, Sargis Sedrakyan,
Stafano Da Sacco,
and Roger
De Filippo
I. Introduction
86
II.
Amniotic
Fluid Cell Composition and Stem Cells
87
III. In Vitro Potential of
Amniotic
Fluid Stem Cells
91
IV. Ex Vivo and In Vivo Application of
Amniotic
Fluid Stem Cells
94
V. Discussion
97
References
98
6.
Method to Isolate Mesenchymal-Like Cells from Wharton s Jelly of Umbilical Cord
Kiran Seshareddy, Deryl Troyer, and Mark L. Weiss
I. Introduction
102
II. Rationale
103
III. Methods
103
IV. Materials
110
V. UCMSCs and Culture Characteristics
115
VI. Discussion
116
References
118
7.
Isolation, Characterization, and Differentiation of Human Umbilical Cord
Perivascular Cells (HUCPVCs)
J. Ennis, R. Samgaser, A. Gomez, Dolores Baksh, and John E. Davies
I. Introduction
122
II. Methods
123
HI. Concluding Remarks
133
References
134
Contents
Hepatic
Stem
Cells and Hepatoblasts: Identification, Isolation, and
Ex Vivo Maintenance
Eliane Wauthier, Eva
Schmelzer, William
Turner,
Uli
Zhang, Ed LeCluyse,
Joseph Ruiz,
Rachetei
Turner, M. E. Furth, Hiroshi Kubota, Oswaldo Lozoya,
Claire
Barbier,
Randall McClelland, Hsin-lei Yao, Nicholas Moss, Andrew Bruce,
John Ludhw, and L. M. Reid
I. Introduction
139
II. Epithelial—Mesenchymal Relationship
139
III. Stem Cells and Lineage Biology
140
IV. Liver Processing
150
V. Fractionation of Liver Cell
Subpopulations
162
VI. Feeder Cells
170
VII.
Extracellular Matrix
174
VIII.
Culture Media
176
IX. Monolayer Cultures of Cells
180
X. Three-Dimensional Systems
185
Appendix
1:
Nomenclature, Glossary, and Abbreviations
195
Appendix
2:
Details on the Preparation of Buffers/Reagents
207
Appendix
3:
Commercial Sources of Reagents
211
Appendix
4:
Sources of Primary and Secondary Antibodies
for Immunoselection and for Characterization of the Cells
212
References
213
9.
Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo
Rongliao Li and Jennie P. Mather
I. Introduction
228
II. Rationale
229
III. Methods
229
IV. Materials
237
V. Discussion
237
VI.
Summan
238
References
239
10.
Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells
Deryk L·™, Claude Behejar, Jeff Hooley, and Xiaoltn
Хи
I. Introduction
242
II. Rationale
243
III. Methods
245
IV. Materials
250
V. Discussion
253
VI. Summary
254
References
254
Contents
11. Isolation
of
Human Mesenchymal
Stem Cells from Bone and Adipose Tissue
Susan H. Bernacki, Michelle E. Wall, and Elizabeth G. Loboa
I. Introduction
258
II. Isolation of hMSCs from Trabecular Bone
259
III. Isolation of hMSCs from Adipose Tissue
263
IV. Growth, Subculture, and Cryopreservation of UndifFerentiated hMSCs
265
V. Surface Marker Characterization of hMSCs
267
VI. hMSC Differentiation Assays for Osteogenic, Adipogenic,
and Chondrogenic Pathways
270
VII.
Summary
276
References
276
12.
Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions
Dolores Baksh and John E. Davies
I. Introduction
280
II. Suspension Culture of Bone Marrow-Derived MPCs
280
HI. Analysis of Suspension Culture
283
IV. Mechanism of
MPC
Expansion in Suspension
288
V. Discussion
291
References
292
13.
Purification and
Long-Term
Culture of
Multipotent
Progenitor Cells Affiliated with
the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes
Mihaela
Clisan,
Bridget Deasy,
Manuela Gavina,
Bo Zheng, Johnny Huard,
Lorenza
Lazzari,
and Bruno
Péault
I. Introduction
297
II. Materials and Methods: Cell Analysis and Purification by Flow Cytometry
298
III. Materials and Methods:
Long-Term
Culture of Human Blood
Vessel-Associated Progenitor Cells
301
IV. Materials and Methods: Proliferation Kinetics of Human
Myoendothelial Cells and Pericytes
303
V. Materials and Methods:
Genotypie
and Phenotypic Analyses
of
Long-Term
Cultured Cells
304
VI. Conclusion
306
References
308
14.
Isolation and Culture of Colon Cancer Stem Cells
Patrizia
Cammanri, Ylenia
Lombardo,
Maria
Giovanna Fraticipane,
Sebastino Bonventre,
Matilde
Todaro,
and Giorgio
Stassi
I. Introduction
312
II. Identification of CSCs in Colon Cancer Tissue
314
Contents
III.
Isolation and
Propagation
of Colon CSCs
315
IV. Discussion
322
References
323
15.
Isolation and Establishment of Human Tumor Stem Cells
Penelope E. Roberts
I. Introduction
326
II. Rationale
327
III. Methods
327
IV. Results
336
V. Discussion
338
VI. Materials
340
References
341
16.
Stem Cells from Cartilaginous and Bony Fish
David W. Barnes, Angela
Partou,
Mitsuru
Tomana
Jae-Ho Hwang,
Anne Czechanski, Lanchtm Fan, and
Patii Collodi
I. Introduction to Models and Uses
344
II. Embryonal Stem Cells
348
III. Tissue-Specific Stem Cells
353
IV. Outlook and Future Contributions
361
References
362
Index
369
Volumes in Series
379
|
| adam_txt |
CONTENTS
Contributors
xi
Preface
xv
Biography
xix
1.
Derivation of Human Embryonic Stem Cells in Standard
and Chemically Defined Conditions
Eric Cimo,
Muriel
Kmet,
Barry Belir, and Julie Baker
I. Introduction
1
II. Methods
2
III. Discussion
12
IV. Materials
12
References
13
2.
Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic
Stem Cells in Feeder and Feeder-Free Conditions
Andre Choo,
Ang
Sheii Ngo, Vanessa Ding, Steve Oh, and
Lim
Sai
Kimig
I. Introduction
16
II. Methods and Materials
17
III. Results
21
IV. Discussion and Summary
25
References
28
3.
Microporous Membrane Growth Substrates for Embryonic Stem Cell
Culture and Differentiation
Steven D. Sheridan,
Sonia
Gii,
Matthew
Wilgo,
and
Aldo Pitt
I. Introduction
30
II. Rationale
32
HI. Methods
32
IV. Summary
54
References
55
Contents
4.
Culture
of
Rodent
Spermatogonial Stem Cells, Male Germline Stem Cells
of the Postnatal Animal
Hiroshi Kubota and Ralph L. Brinster
I. Introduction
60
II. Rationale
63
III. Methods
67
IV. Materials
78
V. Discussion
80
References
81
5.
Characterization of Human
Amniotic
Fluid Stem Cells
and Their Pluripotential Capability
Laura Venn, Sargis Sedrakyan,
Stafano Da Sacco,
and Roger
De Filippo
I. Introduction
86
II.
Amniotic
Fluid Cell Composition and Stem Cells
87
III. In Vitro Potential of
Amniotic
Fluid Stem Cells
91
IV. Ex Vivo and In Vivo Application of
Amniotic
Fluid Stem Cells
94
V. Discussion
97
References
98
6.
Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical Cord
Kiran Seshareddy, Deryl Troyer, and Mark L. Weiss
I. Introduction
102
II. Rationale
103
III. Methods
103
IV. Materials
110
V. UCMSCs and Culture Characteristics
115
VI. Discussion
116
References
118
7.
Isolation, Characterization, and Differentiation of Human Umbilical Cord
Perivascular Cells (HUCPVCs)
J. Ennis, R. Samgaser, A. Gomez, Dolores Baksh, and John E. Davies
I. Introduction
122
II. Methods
123
HI. Concluding Remarks
133
References
134
Contents
Hepatic
Stem
Cells and Hepatoblasts: Identification, Isolation, and
Ex Vivo Maintenance
Eliane Wauthier, Eva
Schmelzer, William
Turner,
Uli
Zhang, Ed LeCluyse,
Joseph Ruiz,
Rachetei
Turner, M. E. Furth, Hiroshi Kubota, Oswaldo Lozoya,
Claire
Barbier,
Randall McClelland, Hsin-lei Yao, Nicholas Moss, Andrew Bruce,
John Ludhw, and L. M. Reid
I. Introduction
139
II. Epithelial—Mesenchymal Relationship
139
III. Stem Cells and Lineage Biology
140
IV. Liver Processing
150
V. Fractionation of Liver Cell
Subpopulations
162
VI. Feeder Cells
170
VII.
Extracellular Matrix
174
VIII.
Culture Media
176
IX. Monolayer Cultures of Cells
180
X. Three-Dimensional Systems
185
Appendix
1:
Nomenclature, Glossary, and Abbreviations
195
Appendix
2:
Details on the Preparation of Buffers/Reagents
207
Appendix
3:
Commercial Sources of Reagents
211
Appendix
4:
Sources of Primary and Secondary Antibodies
for Immunoselection and for Characterization of the Cells
212
References
213
9.
Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo
Rongliao Li and Jennie P. Mather
I. Introduction
228
II. Rationale
229
III. Methods
229
IV. Materials
237
V. Discussion
237
VI.
Summan'
238
References
239
10.
Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells
Deryk L·™, Claude Behejar, Jeff Hooley, and Xiaoltn
Хи
I. Introduction
242
II. Rationale
243
III. Methods
245
IV. Materials
250
V. Discussion
253
VI. Summary
254
References
254
Contents
11. Isolation
of
Human Mesenchymal
Stem Cells from Bone and Adipose Tissue
Susan H. Bernacki, Michelle E. Wall, and Elizabeth G. Loboa
I. Introduction
258
II. Isolation of hMSCs from Trabecular Bone
259
III. Isolation of hMSCs from Adipose Tissue
263
IV. Growth, Subculture, and Cryopreservation of UndifFerentiated hMSCs
265
V. Surface Marker Characterization of hMSCs
267
VI. hMSC Differentiation Assays for Osteogenic, Adipogenic,
and Chondrogenic Pathways
270
VII.
Summary
276
References
276
12.
Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions
Dolores Baksh and John E. Davies
I. Introduction
280
II. Suspension Culture of Bone Marrow-Derived MPCs
280
HI. Analysis of Suspension Culture
283
IV. Mechanism of
MPC
Expansion in Suspension
288
V. Discussion
291
References
292
13.
Purification and
Long-Term
Culture of
Multipotent
Progenitor Cells Affiliated with
the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes
Mihaela
Clisan,
Bridget Deasy,
Manuela Gavina,
Bo Zheng, Johnny Huard,
Lorenza
Lazzari,
and Bruno
Péault
I. Introduction
297
II. Materials and Methods: Cell Analysis and Purification by Flow Cytometry
298
III. Materials and Methods:
Long-Term
Culture of Human Blood
Vessel-Associated Progenitor Cells
301
IV. Materials and Methods: Proliferation Kinetics of Human
Myoendothelial Cells and Pericytes
303
V. Materials and Methods:
Genotypie
and Phenotypic Analyses
of
Long-Term
Cultured Cells
304
VI. Conclusion
306
References
308
14.
Isolation and Culture of Colon Cancer Stem Cells
Patrizia
Cammanri, Ylenia
Lombardo,
Maria
Giovanna Fraticipane,
Sebastino Bonventre,
Matilde
Todaro,
and Giorgio
Stassi
I. Introduction
312
II. Identification of CSCs in Colon Cancer Tissue
314
Contents
III.
Isolation and
Propagation
of Colon CSCs
315
IV. Discussion
322
References
323
15.
Isolation and Establishment of Human Tumor Stem Cells
Penelope E. Roberts
I. Introduction
326
II. Rationale
327
III. Methods
327
IV. Results
336
V. Discussion
338
VI. Materials
340
References
341
16.
Stem Cells from Cartilaginous and Bony Fish
David W. Barnes, Angela
Partou,
Mitsuru
Tomana
Jae-Ho Hwang,
Anne Czechanski, Lanchtm Fan, and
Patii Collodi
I. Introduction to Models and Uses
344
II. Embryonal Stem Cells
348
III. Tissue-Specific Stem Cells
353
IV. Outlook and Future Contributions
361
References
362
Index
369
Volumes in Series
379 |
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| indexdate | 2024-07-09T21:19:39Z |
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| series2 | Methods in cell biology |
| spelling | Stem cell culture ed. by Jennie P. Mather Amsterdam [u.a.] Elsevier Acad. Press 2008 XIX, 386 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 86 Cell Culture Techniques Stem Cells Stem cells Stem cells Research Embryonale Stammzelle (DE-588)4699648-5 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Embryonale Stammzelle (DE-588)4699648-5 s Zellkultur (DE-588)4067547-6 s DE-604 Mather, Jennie P. Sonstige oth Methods in cell biology 86 (DE-604)BV002534878 86 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016659148&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stem cell culture Methods in cell biology Cell Culture Techniques Stem Cells Stem cells Stem cells Research Embryonale Stammzelle (DE-588)4699648-5 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4699648-5 (DE-588)4067547-6 (DE-588)4143413-4 |
| title | Stem cell culture |
| title_auth | Stem cell culture |
| title_exact_search | Stem cell culture |
| title_exact_search_txtP | Stem cell culture |
| title_full | Stem cell culture ed. by Jennie P. Mather |
| title_fullStr | Stem cell culture ed. by Jennie P. Mather |
| title_full_unstemmed | Stem cell culture ed. by Jennie P. Mather |
| title_short | Stem cell culture |
| title_sort | stem cell culture |
| topic | Cell Culture Techniques Stem Cells Stem cells Stem cells Research Embryonale Stammzelle (DE-588)4699648-5 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Cell Culture Techniques Stem Cells Stem cells Stem cells Research Embryonale Stammzelle Zellkultur Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016659148&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT matherjenniep stemcellculture |