Neural stem cells: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2008
|
| Edition: | 2. ed. |
| Series: | Methods in molecular biology
438 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Cover Inhaltsverzeichnis |
| Physical Description: | XV, 406 S. Ill., graph. Darst. |
| ISBN: | 9781588298461 9781597451338 |
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| 245 | 1 | 0 | |a Neural stem cells |b methods and protocols |c ed. by Leslie P. Weiner |
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| adam_text | Contents
Preface v
Contributors xi
Part I: Introduction
1 Definitions and Criteria for Stem Cells
Leslie P. Weiner 3
2 Neural Stem Cells and the Future Treatment of Neurological
Diseases: Raising the Standard
Jaime I mi tola and Samia J. Khoury 9
Part II: Isolation and Culture of NSCs
3 Neural Differentiation of Human Embryonic Stem Cells
Mirella Dottori and Martin F. Pera 19
4 Generating Neurons from Stem Cells
Andreas Androutsellis-Theotokis, Sachiko Murase,
Justin D. Boyd, Deric M. Park, Daniel J. Hoeppner,
Rea Ravin, and Ronald D. G. McKay 31
5 Isolation of Neural Stem and Precursor Cells from Rodent Tissue
Yuanyuan Wu, Ying Liu, Jonathan D. Chesnut,
and Mahendra S. Rao 39
6 Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation
and Characterization
Lamya S. Shihabuddin JJ
7 Adult Neural Stem Cells
Rossella Galli, Angela Gritti, and Angelo L. Vescovi 67
8 Olfactory Ensheathing Cells: Isolation and Culture
from the Neonatal Olfactory Bulb
Susan C. Barnett and A. Jane Roskams 85
9 Culturing Olfactory Ensheathing Cells from the Mouse Olfactory
Epithelium
Miranda Richter, Kathryn Westendorf, and A. Jane Roskams 95
vii
viii Contents
10 Production and Characterization of Immortal Human Neural
Stem Cell Line with Multipotent Differentiation Property
Seung U. Kim, Atsushi Nagai, Eiji Nakagawa, Hyun B. Choi,
Jung H. Bang, Hong J. Lee, Myung A. Lee, Yong B. Lee,
and In H. Park 103
11 Preparation of Neural Progenitors from Bone Marrow
and Umbilical Cord Blood
Shijie Song and Juan Sanchez-Ramos 123
12 Production of Neurospheres from CNS Tissue
Gregory P. Marshall II, Heather H. Ross, Oleg Suslov,
long Zheng, Dennis A. Steindler, and Eric D. Laywell 135
13 Nuclear Transfer to Study the Nuclear Reprogramming of Human
Stem Cells
Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda,
and Kazunari Yokoyama 151
Part III: In Vitro Studies: A. Cellular Techniques
14 Clonal Analyses and Cryopreservation of Neural Stem
Cell Cultures
Angela Gritti, Rossella Galli, and Angelo L. Vescovi 173
15 Telomere Neurobiology
Mark P. Mattson, Peisu Zhang, and Aiwu Cheng 185
16 Chromosomal Mosaicism in Neural Stem Cells
Suzanne E. Peterson, Jurjen W. Westra, Christine M. Paczkowski,
andjerold Chun 197
17 In Vitro Assays for Neural Stem Cell Differentiation: Induction
of Dopaminergic Phenotype
Marcel M. Daadi 205
18 In Vitro Migration Assays of Neural Stem Cells
Pascale Durbec, Isabelle Franceschini, Francoise Lazarini,
and Monique Dubois-Dalcq 213
19 Measuring Apoptosis in Neural Stem Cells
Brett T. Lund and Eve E. Kelland 227
20 Markers of Adult Neural Stem Cells
Eric Wexler 243
Part III: In Vitro Studies: B. Molecular Techniques
21 Differential Gene Expression in ES-Derived Neural Stem Cells
by Using RT-PCR
Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch 271
Contents ix
22 Microfluidic Devices for High-Throughput Gene Expression
Profiling of Single hESC-Derived Neural Stem Cells
Yan Chen and Jiang F. Zhong 293
23 Electroporation Strategies for Genetic Manipulation and Cell
Labeling
Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark... 305
24 Novel and Immortalization-Based Protocols for the Generation
of Neural CNS Stem Cell Lines for Gene Therapy Approaches
Luciano Conti and Elena Cattaneo 319
Part IV: Studies for Transplantation
25 Identification of Newborn Cells by BrdU Labeling
and Immunocytochemistry In Vivo
Sanjay S. Magavi and Jeffrey D. Macklis 335
26 Immunocytochemical Analysis of Neuronal Differentiation
Sanjay S. Magavi and Jeffrey D. Macklis 345
27 Neuroanatomical Tracing of Neuronal Projections
with Fluoro-Gold
Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis 353
28 Labeling Stem Cells In Vitro for Identification of their
Differentiated Phenotypes after Grafting into the CNS
Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore 36/
29 Optimizing Stem Cell Grafting into the CNS
Scott R. Whittemore, Yi Ping Zhang, Christopher B. Shields,
and David S. K. Magnuson 375
30 Routes of Stem Cell Administration in the Adult Rodent
Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg,
and Samuel Saporta 383
Index 403
|
| adam_txt |
Contents
Preface v
Contributors xi
Part I: Introduction
1 Definitions and Criteria for Stem Cells
Leslie P. Weiner 3
2 Neural Stem Cells and the Future Treatment of Neurological
Diseases: Raising the Standard
Jaime I mi tola and Samia J. Khoury 9
Part II: Isolation and Culture of NSCs
3 Neural Differentiation of Human Embryonic Stem Cells
Mirella Dottori and Martin F. Pera 19
4 Generating Neurons from Stem Cells
Andreas Androutsellis-Theotokis, Sachiko Murase,
Justin D. Boyd, Deric M. Park, Daniel J. Hoeppner,
Rea Ravin, and Ronald D. G. McKay 31
5 Isolation of Neural Stem and Precursor Cells from Rodent Tissue
Yuanyuan Wu, Ying Liu, Jonathan D. Chesnut,
and Mahendra S. Rao 39
6 Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation
and Characterization
Lamya S. Shihabuddin JJ
7 Adult Neural Stem Cells
Rossella Galli, Angela Gritti, and Angelo L. Vescovi 67
8 Olfactory Ensheathing Cells: Isolation and Culture
from the Neonatal Olfactory Bulb
Susan C. Barnett and A. Jane Roskams 85
9 Culturing Olfactory Ensheathing Cells from the Mouse Olfactory
Epithelium
Miranda Richter, Kathryn Westendorf, and A. Jane Roskams 95
vii
viii Contents
10 Production and Characterization of Immortal Human Neural
Stem Cell Line with Multipotent Differentiation Property
Seung U. Kim, Atsushi Nagai, Eiji Nakagawa, Hyun B. Choi,
Jung H. Bang, Hong J. Lee, Myung A. Lee, Yong B. Lee,
and In H. Park 103
11 Preparation of Neural Progenitors from Bone Marrow
and Umbilical Cord Blood
Shijie Song and Juan Sanchez-Ramos 123
12 Production of Neurospheres from CNS Tissue
Gregory P. Marshall II, Heather H. Ross, Oleg Suslov,
long Zheng, Dennis A. Steindler, and Eric D. Laywell 135
13 Nuclear Transfer to Study the Nuclear Reprogramming of Human
Stem Cells
Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda,
and Kazunari Yokoyama 151
Part III: In Vitro Studies: A. Cellular Techniques
14 Clonal Analyses and Cryopreservation of Neural Stem
Cell Cultures
Angela Gritti, Rossella Galli, and Angelo L. Vescovi 173
15 Telomere Neurobiology
Mark P. Mattson, Peisu Zhang, and Aiwu Cheng 185
16 Chromosomal Mosaicism in Neural Stem Cells
Suzanne E. Peterson, Jurjen W. Westra, Christine M. Paczkowski,
andjerold Chun 197
17 In Vitro Assays for Neural Stem Cell Differentiation: Induction
of Dopaminergic Phenotype
Marcel M. Daadi 205
18 In Vitro Migration Assays of Neural Stem Cells
Pascale Durbec, Isabelle Franceschini, Francoise Lazarini,
and Monique Dubois-Dalcq 213
19 Measuring Apoptosis in Neural Stem Cells
Brett T. Lund and Eve E. Kelland 227
20 Markers of Adult Neural Stem Cells
Eric Wexler 243
Part III: In Vitro Studies: B. Molecular Techniques
21 Differential Gene Expression in ES-Derived Neural Stem Cells
by Using RT-PCR
Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch 271
Contents ix
22 Microfluidic Devices for High-Throughput Gene Expression
Profiling of Single hESC-Derived Neural Stem Cells
Yan Chen and Jiang F. Zhong 293
23 Electroporation Strategies for Genetic Manipulation and Cell
Labeling
Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark. 305
24 Novel and Immortalization-Based Protocols for the Generation
of Neural CNS Stem Cell Lines for Gene Therapy Approaches
Luciano Conti and Elena Cattaneo 319
Part IV: Studies for Transplantation
25 Identification of Newborn Cells by BrdU Labeling
and Immunocytochemistry In Vivo
Sanjay S. Magavi and Jeffrey D. Macklis 335
26 Immunocytochemical Analysis of Neuronal Differentiation
Sanjay S. Magavi and Jeffrey D. Macklis 345
27 Neuroanatomical Tracing of Neuronal Projections
with Fluoro-Gold
Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis 353
28 Labeling Stem Cells In Vitro for Identification of their
Differentiated Phenotypes after Grafting into the CNS
Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore 36/
29 Optimizing Stem Cell Grafting into the CNS
Scott R. Whittemore, Yi Ping Zhang, Christopher B. Shields,
and David S. K. Magnuson 375
30 Routes of Stem Cell Administration in the Adult Rodent
Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg,
and Samuel Saporta 383
Index 403 |
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| spelling | Neural stem cells methods and protocols ed. by Leslie P. Weiner 2. ed. Totowa, NJ Humana Press 2008 XV, 406 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 438 Cytological Techniques Laboratory Manuals Neurons Laboratory manuals Neurons cytology Laboratory Manuals Stem Cells cytology Laboratory Manuals Stem Cells physiology Laboratory Manuals Stem cells Laboratory manuals Labortechnik (DE-588)4123602-6 gnd rswk-swf Nervenstammzelle (DE-588)4714646-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Nervenstammzelle (DE-588)4714646-1 s Labortechnik (DE-588)4123602-6 s DE-604 Weiner, Leslie P. Sonstige oth Methods in molecular biology 438 (DE-604)BV035362695 438 http://media.obvsg.at/AC06654286-1001 UBG Inhaltsverzeichnis http://media.obvsg.at/AC06654286-4001 UBG Cover HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016658463&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Neural stem cells methods and protocols Methods in molecular biology Cytological Techniques Laboratory Manuals Neurons Laboratory manuals Neurons cytology Laboratory Manuals Stem Cells cytology Laboratory Manuals Stem Cells physiology Laboratory Manuals Stem cells Laboratory manuals Labortechnik (DE-588)4123602-6 gnd Nervenstammzelle (DE-588)4714646-1 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4714646-1 (DE-588)4143413-4 |
| title | Neural stem cells methods and protocols |
| title_auth | Neural stem cells methods and protocols |
| title_exact_search | Neural stem cells methods and protocols |
| title_exact_search_txtP | Neural stem cells methods and protocols |
| title_full | Neural stem cells methods and protocols ed. by Leslie P. Weiner |
| title_fullStr | Neural stem cells methods and protocols ed. by Leslie P. Weiner |
| title_full_unstemmed | Neural stem cells methods and protocols ed. by Leslie P. Weiner |
| title_short | Neural stem cells |
| title_sort | neural stem cells methods and protocols |
| title_sub | methods and protocols |
| topic | Cytological Techniques Laboratory Manuals Neurons Laboratory manuals Neurons cytology Laboratory Manuals Stem Cells cytology Laboratory Manuals Stem Cells physiology Laboratory Manuals Stem cells Laboratory manuals Labortechnik (DE-588)4123602-6 gnd Nervenstammzelle (DE-588)4714646-1 gnd |
| topic_facet | Cytological Techniques Laboratory Manuals Neurons Laboratory manuals Neurons cytology Laboratory Manuals Stem Cells cytology Laboratory Manuals Stem Cells physiology Laboratory Manuals Stem cells Laboratory manuals Labortechnik Nervenstammzelle Aufsatzsammlung |
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| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT weinerlesliep neuralstemcellsmethodsandprotocols |
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