Two-focus fluorescence correlation spectroscopy:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Jülich
Forschungszentrum, Zentralbibliothek
2007
|
| Series: | Berichte des Forschungszentrums Jülich
4250 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Auch im Internet unter der Adresse http://www.fz-juelich.de/zb/juwel verfügbar |
| Physical Description: | IV, 86 S. Ill., graph. Darst. 30 cm |
Staff View
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| 245 | 1 | 0 | |a Two-focus fluorescence correlation spectroscopy |c Thomas Dertinger. [Forschungszentrum Jülich in der Helmholtz-Gemeinschaft, Institut für Neurowissenschaften und Biophysik (INB), Zelluläre Biophysik (INB-1)] |
| 264 | 1 | |a Jülich |b Forschungszentrum, Zentralbibliothek |c 2007 | |
| 300 | |a IV, 86 S. |b Ill., graph. Darst. |c 30 cm | ||
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| 502 | |a Zugl.: Köln, Univ., Diss., 2007 | ||
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Record in the Search Index
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| adam_text | Content
1. Abstract ffl
2. Zusammenfassung IV
3. Introduction 1
4. Basic features of two-focus FCS 9
4.1. Working principle and setup 9
4.2. Measuring and fitting the molecule detection function 14
4.3. Determining the distance of the foci 18
5. Diffusion of Atto655 and Cy5 under various conditions 23
5.7. Refractive Index Mismatch 26
5.2. Optical Saturation 30
6. Planar diffusion 33
6.1. Diffusion in supported phospholipid-bilayers 34
6.2. Diffusion in the membrane of giant unilamellar vesicles 38
7. Proteins 40
7.1. Conformational changes of calmodulin 40
7.2. Conformational changes of recoverin 48
7.3. Unfolding the Tryptophan-Cage 57
8. Summary 56
9. Acknowledgements 60
10. Appendix 64
70.7. General aspects of the autocorrelation function 65
10.2. Extending the theory to two-focus FCS 69
10.3. Finding a good description for the MDF. 70
10.4. Evaluation of the resulting correlation function 72
10.5. Free, two-dimensional, planar diffusion 74
10.6. Surface adsorption/desorption in planar systems 77
10.7. General considerations: Microsecond blinking 79
11. Materials and Methods 81
12. Acronyms 87
13. References 89
|
| adam_txt |
Content
1. Abstract ffl
2. Zusammenfassung IV
3. Introduction 1
4. Basic features of two-focus FCS 9
4.1. Working principle and setup 9
4.2. Measuring and fitting the molecule detection function 14
4.3. Determining the distance of the foci 18
5. Diffusion of Atto655 and Cy5 under various conditions 23
5.7. Refractive Index Mismatch 26
5.2. Optical Saturation 30
6. Planar diffusion 33
6.1. Diffusion in supported phospholipid-bilayers 34
6.2. Diffusion in the membrane of giant unilamellar vesicles 38
7. Proteins 40
7.1. Conformational changes of calmodulin 40
7.2. Conformational changes of recoverin 48
7.3. Unfolding the Tryptophan-Cage 57
8. Summary 56
9. Acknowledgements 60
10. Appendix 64
70.7. General aspects of the autocorrelation function 65
10.2. Extending the theory to two-focus FCS 69
10.3. Finding a good description for the MDF. 70
10.4. Evaluation of the resulting correlation function 72
10.5. Free, two-dimensional, planar diffusion 74
10.6. Surface adsorption/desorption in planar systems 77
10.7. General considerations: Microsecond blinking 79
11. Materials and Methods 81
12. Acronyms 87
13. References 89 |
| any_adam_object | 1 |
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| author | Dertinger, Thomas |
| author_facet | Dertinger, Thomas |
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| author_sort | Dertinger, Thomas |
| author_variant | t d td |
| building | Verbundindex |
| bvnumber | BV023393014 |
| ctrlnum | (OCoLC)181589648 (DE-599)DNB984902899 |
| dewey-full | 535.355 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 535 - Light and related radiation |
| dewey-raw | 535.355 |
| dewey-search | 535.355 |
| dewey-sort | 3535.355 |
| dewey-tens | 530 - Physics |
| discipline | Physik |
| discipline_str_mv | Physik |
| format | Thesis Book |
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| illustrated | Illustrated |
| index_date | 2024-07-02T21:20:43Z |
| indexdate | 2024-07-09T21:17:35Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016575922 |
| oclc_num | 181589648 |
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| physical | IV, 86 S. Ill., graph. Darst. 30 cm |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Forschungszentrum, Zentralbibliothek |
| record_format | marc |
| series | Berichte des Forschungszentrums Jülich |
| series2 | Berichte des Forschungszentrums Jülich |
| spelling | Dertinger, Thomas Verfasser aut Two-focus fluorescence correlation spectroscopy Thomas Dertinger. [Forschungszentrum Jülich in der Helmholtz-Gemeinschaft, Institut für Neurowissenschaften und Biophysik (INB), Zelluläre Biophysik (INB-1)] Jülich Forschungszentrum, Zentralbibliothek 2007 IV, 86 S. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Berichte des Forschungszentrums Jülich 4250 Auch im Internet unter der Adresse http://www.fz-juelich.de/zb/juwel verfügbar Zugl.: Köln, Univ., Diss., 2007 Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 s DE-604 Berichte des Forschungszentrums Jülich 4250 (DE-604)BV004194732 4250 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016575922&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dertinger, Thomas Two-focus fluorescence correlation spectroscopy Berichte des Forschungszentrums Jülich Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 gnd |
| subject_GND | (DE-588)4636290-3 (DE-588)4113937-9 |
| title | Two-focus fluorescence correlation spectroscopy |
| title_auth | Two-focus fluorescence correlation spectroscopy |
| title_exact_search | Two-focus fluorescence correlation spectroscopy |
| title_exact_search_txtP | Two-focus fluorescence correlation spectroscopy |
| title_full | Two-focus fluorescence correlation spectroscopy Thomas Dertinger. [Forschungszentrum Jülich in der Helmholtz-Gemeinschaft, Institut für Neurowissenschaften und Biophysik (INB), Zelluläre Biophysik (INB-1)] |
| title_fullStr | Two-focus fluorescence correlation spectroscopy Thomas Dertinger. [Forschungszentrum Jülich in der Helmholtz-Gemeinschaft, Institut für Neurowissenschaften und Biophysik (INB), Zelluläre Biophysik (INB-1)] |
| title_full_unstemmed | Two-focus fluorescence correlation spectroscopy Thomas Dertinger. [Forschungszentrum Jülich in der Helmholtz-Gemeinschaft, Institut für Neurowissenschaften und Biophysik (INB), Zelluläre Biophysik (INB-1)] |
| title_short | Two-focus fluorescence correlation spectroscopy |
| title_sort | two focus fluorescence correlation spectroscopy |
| topic | Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 gnd |
| topic_facet | Fluoreszenzkorrelationsspektroskopie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016575922&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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| work_keys_str_mv | AT dertingerthomas twofocusfluorescencecorrelationspectroscopy |