Fluorescence methods and applications: spectroscopy, imaging, and probes
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Boston, Mass.
Blackwell
2008
|
| Series: | Annals of the New York Academy of Sciences
1130 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XI, 328 S. Ill., graph. Darst. |
| ISBN: | 9781573317160 |
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| 245 | 1 | 0 | |a Fluorescence methods and applications |b spectroscopy, imaging, and probes |c ed. by Otto S. Wolfbeis |
| 264 | 1 | |a Boston, Mass. |b Blackwell |c 2008 | |
| 300 | |a XI, 328 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Annals of the New York Academy of Sciences |v 1130 | |
| 650 | 4 | |a Biosensors |v Congresses | |
| 650 | 4 | |a Fluorescence spectroscopy |v Congresses | |
| 650 | 4 | |a Fluorescent probes |v Congresses | |
| 650 | 4 | |a Luminescence spectroscopy |v Congresses | |
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ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Volume 1130
Fluorescence Methods and Applications:
Spectroscopy, Imaging, and Probes
Editor
Otto S. Woi.kbeis
This volume is the result of the 10th Conference on Methods and Applications of Fluorescence:
Spectroscopy, Imaging, and Probes held September 9 12, 2007 at the Salzburg Congress Outer,
Salzburg, Austria.
CONTENTS
Preface. By Otto S. Wolfbeis xi
Part I. Fluorescence in High-Throughput Screening
Single-bead, Single-molecule, Single-cell Fluorescence: Technologies lor
Drug Screening and Target Validation. By Martin Hintersteiner
and Manfred Auer 1
Substrate Arrays for Fluorescence-Based Enzyme Fingerprinting and
High-Throughput Screening. By Jean-Louis Reymond 12
Part II. Fluorescence Spectroscopy
Probing the Interior of Living Cells with Fluorescence Correlation
Spectroscopy. By Matthias Weiss 21
Fluorescence Measurements on Functionalizcd Polymer Surfaces -Problems and
Troubleshooting. By Katrin Hoffmann, Rcnatc Mix. Ute Resch-Genger,
and Joerg F. Friedrich 28
vii
viii Annals of the New York Academy of Sciences
Standardization of" Fluorescence Measurements: Criteria for the Choice of
Suitable Standards and Approaches to Fit-Cor-Purpose Calibration Tools. By
L'tc Resch-Genger. Katrin Hoffmann, and Angclika HofFmann 35
Investigating Transcriptional Regulation by Muorescence Speclroscopy, from
Traditional Methods to State-of-the-Art Single-Molecule Approaches. By
Silvia Zorrilla. M. Pilar Lillo. Denis Chaix. Emmanuel Margeat, Catherine
A. Rover, and Nathalie Declerck 44
Subpicosecond Transient Signal Spectroscopy of Prodan in Dimcthylformamide
Solution. By Janos Erostyak, Pasi Myllyperkio, Andrea Buzady,
and Jouko Korppi-Tommola 52
Frequency Domain Fluorometry with Pulsed Light-Emitting Diodes. By Pctr
Herman andjaroslav Yecer 56
Toward Smgle-inetal-ion Sensing by torster Resonance Energy Transfer. By
Jens L*. Sutler. Alexander Macmillan. David }. S. Birch, and
OlafJ. Rolinski ' 62
Fluorescence Interferometry: Principles and Applications in Biology. By Alberto
Bilenca, Jing Cao. Max Colice. Aydogan Ozcan, Brett Bouma,
Laurel Raftery. and Guillermo Tearney 68
Acoustically Levitated Droplets: A Contaetlcss Sampling Method for
Fluorescence Studies, /iyjork Lciterer, Markus Grabolle, Knut Rurack,
Lte Resch-Genger, Jan Zieglcr, Thomas Nann, and Ulrich Panne 78
Structural Effects of Biologically Relevant Rhodamines on Spectroscopy of
Fluorescence Fluctuations. By Jose A. B. Ferrcira 85
Part III. Fluorescence Imaging
Dextrin-Microencapsulated Porphyrin: Luminescent Properties. By Priscilla Paiva
Luz. Claudio Roberto Xeri. and Osvaldo Antonio Serra 91
Lanthanide Bimetallic Hclicatcs for in Vitro Imaging and Sensing. By Jean-Claude
G. Biinzli, Anne-Sophie Chau\tn, C'arohne D.B. Vandc\'s'\'cr, Song Bo,
and Steve Goinby 97
Time-Ciatcd Luminescence Microscopy. By Russell E. Connally and James
A. Piper '.'. .' 106
I imc-resohed Microspcctrolluoi'omctn' and Fluorescence Imaging Techniques:
Study of Porphyrin-mediated Cellular Uptake of Oligonucleotides. By Petr
Praus. Eva Kocisova, Peter Mojzes, Josef Stepanek, ()li\-ier Seksck, Franck
Surcati. and Pierre-Wcs Turpin 117
Part IV. Fluorescence Probes and Labels
fluorescent DXA Base Modifications and Substitutes: Multiple Fluorophorc
Labeling and the DETEO_Concept. By Hans-Achim Wagenknecht 122
fluorescent Probes for Chemical Transformations on the Single-Molecule Level.
By Grcgorjung. Alexander Schmitt. MichaelaJacob, and Babettc Hinkcldey 131
Luminescent Lanthanide Complexes as Probes for the Determination of Enzyme
Activities. By Corinna M. Spangler. Christian Spanglcr. and Michael
Schaerling " ' ]. 138
Contents ix
Which Are You Watching, an Individual Reactive Oxygen Species or Total
Oxidative Stress?. By Hatsuo Maeda '.\ 149
Probing of Cosolvents in Polymer Latex Materials by Using Solvatochromic
Fluorescence. By Albert M. Brouwer, Tanzccla N. Raja, Koen Biemans,
Tijs Xabuurs, and Ronald Tennebroek 157
Red/Ncar-infrarcd Boron Dipyrromethene Dyes as Strongly Emitting
Fluorophores. By Ana B. Descalzo, Hai-Jun Xu, Zhen Shen, and Knut
Rurack ." 164
Sensitive Terbium Probes for Luminescent Determination of both Alkaline
Phosphatase and Codeine Phosphate. By Axel Duerkop, Darya Aleksandrova,
Yuliya Scripinets, Alia Ycgorova, and Ekateryna Yit\ ukova 172
Fluorescent Probes and Labels for Biomedical Applications. By Leonid Patsenker,
Anatoliy Tatarcts, Olga Kolosova, Olena Obukhova, Yevgeniy Povrozin,
Iryna Fedyunyayeva, lnna Yermolenko. and Kwakl Terpetschnig 1 79
Part V. Fluorescence Micro- and Nanoparticles
Photon Upconvcrsion in Homogeneous Fluorescence-based Bioanalytical
Assays. By Tero Soukka, Terhi Rantanen, and Kairi Kuningas 188
Processing and (Characterization of Gold Xanoparticles lor I'se in Plasmon Probe
Spectroscopy and Microscopy of Biosyslems. By Yu Chen, Jon A. Preece,
and Richard L\ Palmer ' ' " ' 201
Multifunctionalized Bioeompatible Microspheres lor Sensing. By Rosario M.
Sanchez-Martin, Lois Alexander, and Mark Bradle\ 207
Fluorescent Silica Nanoparticles. By Heike Mader. Xiaohua Li. Saved Saleh,
Martin Link, Peter Kele, and Otto S. Wollbeis 218
Thermally Activated Delayed Fluorescence in Fullerenes. By Carlos Baleizao
and Mario N. Berberan-Santos 224
Stability and Fluorescence Quantum Yield of CdSe ZnS Quantum
Dots—Influence of the Thickness of the ZnS Shell. By Markus Grabolle, Jan
Ziegler, Alcxei Merkulov, 'Fhomas Xann. and L'tc Resch-Gengcr 2.'ir)
Size Distributions of Cadmium Snlfide Xanoparticles Obtained from Templating
Methods. By Paulo J. G. Coutinho, Candido A. (i. Mendes. and
IVresa S. V. Rcis 242
Excitation Energy Transfer and Frap])ing in Dye-Loaded Solid Particles. By
Hernan B. Rodriguez and Enrique San Roman 217
Characterization of Water-soluble Luminescent Quanuun Dots by Muorescence
Correlation Spcciroscopy. By Chao(|ing Dong. Xiangxi Huang,
andjicun Ren '!? '.]
Luminescent Amino-funetionalized or l'.rbium-doped Silica Spheres for Biological
Applications. By Francesco Enrichi 2(i2
Preparation and Characterization ol Xanocr\stalhne Zr() A l)' + .Lr' +
L'p-conversion Phosphors. By Iko I lyppanen. Jorma Holsa.Jouko Kankare.
Mika Lastusaari. and Laura Pililgren 267
Xano- and Microparticles of Organic I-'luore^cent \)\\ Sell-organization and
Optical Properties. By Suzanne I¦cry-1'orgucs. Mouhanimad Abyan.
and Jean-Francois Lamere 272
x Annals of the New York Academy of Sciences
Part VI. Biophysical Fluorescence and Bioanalytical Fluorescence
Flow Cytometric FRET Analysis of erbB Receptor Interaction on a
Cell-by-Cell Basis. By Simone Diermeier-Daucher, Max Hasmann,
and Gero Brockhoff 280
Toward Improved Biochips Based on Rolling Circle Amplification—Influences of
the Microenvironment on the Fluorescence Properties of Labeled DNA
Oligonucleotides. By Elke Mayer-Enthart, Julien Sialelli, Knut Rurack,
Ute Resch-Genger, Daniela Koster, and Harald Seitz 287
Fluorescent Studies on Cooperative Binding of Cationic Pheophorbide-a
Derivative to Polyphosphate. By Olga Ryazanova, Igor Voloshin, Igor Dubey
Larisa Dubey, and Victor Zozulya 293
Effect of pH on Aqueous Phenylalanine Studied Using a 265-nm Pulsed
Light-emitting Diode. By Alexander M. Macmillan, Colin D. McGuinness,
Kulwinder Sagoo, David McLoskey John C. Pickup, and David J. S. Birch . 300
Ordered Self-assembly of Protonated Porphyrin Induced by the Aqueous
Environment of Biomimetic Systems. By Suzana M. Andrade and
Silvia M. B. Costa 305
Human Serum Albumin-flavonoid Interactions Monitored by Means of
Tryptophan Kinetics. By OlafJ. Rolinski, Andrew Martin, and David
J. S. Birch 314
Two-photon Excitation Fluorescence Bioassays. By Pekka Hanninen, Jori Soukka,
and Juhani T. Soini 320
Index of Contributors 327 |
| adam_txt |
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Volume 1130
Fluorescence Methods and Applications:
Spectroscopy, Imaging, and Probes
Editor
Otto S. Woi.kbeis
This volume is the result of the 10th Conference on Methods and Applications of Fluorescence:
Spectroscopy, Imaging, and Probes held September 9 12, 2007 at the Salzburg Congress Outer,
Salzburg, Austria.
CONTENTS
Preface. By Otto S. Wolfbeis xi
Part I. Fluorescence in High-Throughput Screening
Single-bead, Single-molecule, Single-cell Fluorescence: Technologies lor
Drug Screening and Target Validation. By Martin Hintersteiner
and Manfred Auer 1
Substrate Arrays for Fluorescence-Based Enzyme Fingerprinting and
High-Throughput Screening. By Jean-Louis Reymond 12
Part II. Fluorescence Spectroscopy
Probing the Interior of Living Cells with Fluorescence Correlation
Spectroscopy. By Matthias Weiss 21
Fluorescence Measurements on Functionalizcd Polymer Surfaces -Problems and
Troubleshooting. By Katrin Hoffmann, Rcnatc Mix. Ute Resch-Genger,
and Joerg F. Friedrich 28
vii
viii Annals of the New York Academy of Sciences
Standardization of" Fluorescence Measurements: Criteria for the Choice of
Suitable Standards and Approaches to Fit-Cor-Purpose Calibration Tools. By
L'tc Resch-Genger. Katrin Hoffmann, and Angclika HofFmann 35
Investigating Transcriptional Regulation by Muorescence Speclroscopy, from
Traditional Methods to State-of-the-Art Single-Molecule Approaches. By
Silvia Zorrilla. M. Pilar Lillo. Denis Chaix. Emmanuel Margeat, Catherine
A. Rover, and Nathalie Declerck 44
Subpicosecond Transient Signal Spectroscopy of Prodan in Dimcthylformamide
Solution. By Janos Erostyak, Pasi Myllyperkio, Andrea Buzady,
and Jouko Korppi-Tommola 52
Frequency Domain Fluorometry with Pulsed Light-Emitting Diodes. By Pctr
Herman andjaroslav Yecer 56
Toward Smgle-inetal-ion Sensing by torster Resonance Energy Transfer. By
Jens L*. Sutler. Alexander Macmillan. David }. S. Birch, and
OlafJ. Rolinski ' 62
Fluorescence Interferometry: Principles and Applications in Biology. By Alberto
Bilenca, Jing Cao. Max Colice. Aydogan Ozcan, Brett Bouma,
Laurel Raftery. and Guillermo Tearney 68
Acoustically Levitated Droplets: A Contaetlcss Sampling Method for
Fluorescence Studies, /iyjork Lciterer, Markus Grabolle, Knut Rurack,
Lte Resch-Genger, Jan Zieglcr, Thomas Nann, and Ulrich Panne 78
Structural Effects of Biologically Relevant Rhodamines on Spectroscopy of
Fluorescence Fluctuations. By Jose A. B. Ferrcira 85
Part III. Fluorescence Imaging
Dextrin-Microencapsulated Porphyrin: Luminescent Properties. By Priscilla Paiva
Luz. Claudio Roberto Xeri. and Osvaldo Antonio Serra 91
Lanthanide Bimetallic Hclicatcs for in Vitro Imaging and Sensing. By Jean-Claude
G. Biinzli, Anne-Sophie Chau\tn, C'arohne D.B. Vandc\'s'\'cr, Song Bo,
and Steve Goinby 97
Time-Ciatcd Luminescence Microscopy. By Russell E. Connally and James
A. Piper '.'. .' 106
I imc-resohed Microspcctrolluoi'omctn' and Fluorescence Imaging Techniques:
Study of Porphyrin-mediated Cellular Uptake of Oligonucleotides. By Petr
Praus. Eva Kocisova, Peter Mojzes, Josef Stepanek, ()li\-ier Seksck, Franck
Surcati. and Pierre-Wcs Turpin 117
Part IV. Fluorescence Probes and Labels
fluorescent DXA Base Modifications and Substitutes: Multiple Fluorophorc
Labeling and the DETEO_Concept. By Hans-Achim Wagenknecht 122
fluorescent Probes for Chemical Transformations on the Single-Molecule Level.
By Grcgorjung. Alexander Schmitt. MichaelaJacob, and Babettc Hinkcldey 131
Luminescent Lanthanide Complexes as Probes for the Determination of Enzyme
Activities. By Corinna M. Spangler. Christian Spanglcr. and Michael
Schaerling " ' ]. 138
Contents ix
Which Are You Watching, an Individual Reactive Oxygen Species or Total
Oxidative Stress?. By Hatsuo Maeda '.\ 149
Probing of Cosolvents in Polymer Latex Materials by Using Solvatochromic
Fluorescence. By Albert M. Brouwer, Tanzccla N. Raja, Koen Biemans,
Tijs Xabuurs, and Ronald Tennebroek 157
Red/Ncar-infrarcd Boron Dipyrromethene Dyes as Strongly Emitting
Fluorophores. By Ana B. Descalzo, Hai-Jun Xu, Zhen Shen, and Knut
Rurack ." 164
Sensitive Terbium Probes for Luminescent Determination of both Alkaline
Phosphatase and Codeine Phosphate. By Axel Duerkop, Darya Aleksandrova,
Yuliya Scripinets, Alia Ycgorova, and Ekateryna Yit\ ukova 172
Fluorescent Probes and Labels for Biomedical Applications. By Leonid Patsenker,
Anatoliy Tatarcts, Olga Kolosova, Olena Obukhova, Yevgeniy Povrozin,
Iryna Fedyunyayeva, lnna Yermolenko. and Kwakl Terpetschnig 1 79
Part V. Fluorescence Micro- and Nanoparticles
Photon Upconvcrsion in Homogeneous Fluorescence-based Bioanalytical
Assays. By Tero Soukka, Terhi Rantanen, and Kairi Kuningas 188
Processing and (Characterization of Gold Xanoparticles lor I'se in Plasmon Probe
Spectroscopy and Microscopy of Biosyslems. By Yu Chen, Jon A. Preece,
and Richard L\ Palmer ' ' " ' 201
Multifunctionalized Bioeompatible Microspheres lor Sensing. By Rosario M.
Sanchez-Martin, Lois Alexander, and Mark Bradle\ 207
Fluorescent Silica Nanoparticles. By Heike Mader. Xiaohua Li. Saved Saleh,
Martin Link, Peter Kele, and Otto S. Wollbeis 218
Thermally Activated Delayed Fluorescence in Fullerenes. By Carlos Baleizao
and Mario N. Berberan-Santos 224
Stability and Fluorescence Quantum Yield of CdSe ZnS Quantum
Dots—Influence of the Thickness of the ZnS Shell. By Markus Grabolle, Jan
Ziegler, Alcxei Merkulov, 'Fhomas Xann. and L'tc Resch-Gengcr 2.'ir)
Size Distributions of Cadmium Snlfide Xanoparticles Obtained from Templating
Methods. By Paulo J. G. Coutinho, Candido A. (i. Mendes. and
IVresa S. V. Rcis 242
Excitation Energy Transfer and Frap])ing in Dye-Loaded Solid Particles. By
Hernan B. Rodriguez and Enrique San Roman 217
Characterization of Water-soluble Luminescent Quanuun Dots by Muorescence
Correlation Spcciroscopy. By Chao(|ing Dong. Xiangxi Huang,
andjicun Ren '!? '.]
Luminescent Amino-funetionalized or l'.rbium-doped Silica Spheres for Biological
Applications. By Francesco Enrichi 2(i2
Preparation and Characterization ol Xanocr\stalhne Zr() A l)' + .Lr' +
L'p-conversion Phosphors. By Iko I lyppanen. Jorma Holsa.Jouko Kankare.
Mika Lastusaari. and Laura Pililgren 267
Xano- and Microparticles of Organic I-'luore^cent \)\\ Sell-organization and
Optical Properties. By Suzanne I¦cry-1'orgucs. Mouhanimad Abyan.
and Jean-Francois Lamere 272
x Annals of the New York Academy of Sciences
Part VI. Biophysical Fluorescence and Bioanalytical Fluorescence
Flow Cytometric FRET Analysis of erbB Receptor Interaction on a
Cell-by-Cell Basis. By Simone Diermeier-Daucher, Max Hasmann,
and Gero Brockhoff 280
Toward Improved Biochips Based on Rolling Circle Amplification—Influences of
the Microenvironment on the Fluorescence Properties of Labeled DNA
Oligonucleotides. By Elke Mayer-Enthart, Julien Sialelli, Knut Rurack,
Ute Resch-Genger, Daniela Koster, and Harald Seitz 287
Fluorescent Studies on Cooperative Binding of Cationic Pheophorbide-a
Derivative to Polyphosphate. By Olga Ryazanova, Igor Voloshin, Igor Dubey
Larisa Dubey, and Victor Zozulya 293
Effect of pH on Aqueous Phenylalanine Studied Using a 265-nm Pulsed
Light-emitting Diode. By Alexander M. Macmillan, Colin D. McGuinness,
Kulwinder Sagoo, David McLoskey John C. Pickup, and David J. S. Birch . 300
Ordered Self-assembly of Protonated Porphyrin Induced by the Aqueous
Environment of Biomimetic Systems. By Suzana M. Andrade and
Silvia M. B. Costa 305
Human Serum Albumin-flavonoid Interactions Monitored by Means of
Tryptophan Kinetics. By OlafJ. Rolinski, Andrew Martin, and David
J. S. Birch 314
Two-photon Excitation Fluorescence Bioassays. By Pekka Hanninen, Jori Soukka,
and Juhani T. Soini 320
Index of Contributors 327 |
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| genre | (DE-588)1071861417 Konferenzschrift gnd-content |
| genre_facet | Konferenzschrift |
| geographic | Salzburg (DE-588)4076982-3 gnd |
| geographic_facet | Salzburg |
| id | DE-604.BV023392917 |
| illustrated | Illustrated |
| index_date | 2024-07-02T21:20:40Z |
| indexdate | 2025-03-10T11:06:59Z |
| institution | BVB |
| isbn | 9781573317160 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016575831 |
| oclc_num | 239697844 |
| open_access_boolean | |
| owner | DE-12 DE-20 DE-355 DE-BY-UBR |
| owner_facet | DE-12 DE-20 DE-355 DE-BY-UBR |
| physical | XI, 328 S. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Blackwell |
| record_format | marc |
| series | Annals of the New York Academy of Sciences |
| series2 | Annals of the New York Academy of Sciences |
| spelling | Fluorescence methods and applications spectroscopy, imaging, and probes ed. by Otto S. Wolfbeis Boston, Mass. Blackwell 2008 XI, 328 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Annals of the New York Academy of Sciences 1130 Biosensors Congresses Fluorescence spectroscopy Congresses Fluorescent probes Congresses Luminescence spectroscopy Congresses Luminescent probes Congresses Fluoreszenzspektroskopie (DE-588)4017701-4 gnd rswk-swf Salzburg (DE-588)4076982-3 gnd rswk-swf (DE-588)1071861417 Konferenzschrift gnd-content Fluoreszenzspektroskopie (DE-588)4017701-4 s Salzburg (DE-588)4076982-3 g DE-604 Wolfbeis, Otto S. 1947-2023 Sonstige (DE-588)1061164683 oth Annals of the New York Academy of Sciences 1130 (DE-604)BV002532608 1130 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016575831&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fluorescence methods and applications spectroscopy, imaging, and probes Annals of the New York Academy of Sciences Biosensors Congresses Fluorescence spectroscopy Congresses Fluorescent probes Congresses Luminescence spectroscopy Congresses Luminescent probes Congresses Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| subject_GND | (DE-588)4017701-4 (DE-588)4076982-3 (DE-588)1071861417 |
| title | Fluorescence methods and applications spectroscopy, imaging, and probes |
| title_auth | Fluorescence methods and applications spectroscopy, imaging, and probes |
| title_exact_search | Fluorescence methods and applications spectroscopy, imaging, and probes |
| title_exact_search_txtP | Fluorescence methods and applications spectroscopy, imaging, and probes |
| title_full | Fluorescence methods and applications spectroscopy, imaging, and probes ed. by Otto S. Wolfbeis |
| title_fullStr | Fluorescence methods and applications spectroscopy, imaging, and probes ed. by Otto S. Wolfbeis |
| title_full_unstemmed | Fluorescence methods and applications spectroscopy, imaging, and probes ed. by Otto S. Wolfbeis |
| title_short | Fluorescence methods and applications |
| title_sort | fluorescence methods and applications spectroscopy imaging and probes |
| title_sub | spectroscopy, imaging, and probes |
| topic | Biosensors Congresses Fluorescence spectroscopy Congresses Fluorescent probes Congresses Luminescence spectroscopy Congresses Luminescent probes Congresses Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| topic_facet | Biosensors Congresses Fluorescence spectroscopy Congresses Fluorescent probes Congresses Luminescence spectroscopy Congresses Luminescent probes Congresses Fluoreszenzspektroskopie Salzburg Konferenzschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016575831&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002532608 |
| work_keys_str_mv | AT wolfbeisottos fluorescencemethodsandapplicationsspectroscopyimagingandprobes |