Molecular beacons: signalling nucleic acid probes, methods, and protocols
This edited work intends to show a diverse set of instructive examples, from probe design to applications in clinical settings, guided by experts in the field who provide easy-to-follow experimentals.
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana
2008
|
| Schriftenreihe: | Methods in molecular biology
429 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | This edited work intends to show a diverse set of instructive examples, from probe design to applications in clinical settings, guided by experts in the field who provide easy-to-follow experimentals. |
| Beschreibung: | Includes bibliographical references and index Basic principles of fluorescence and energy transfer / Larry E. Morrison -- Multiplex detection of mutations / David S. Perlin, Sergey Balashov, and Steven Park -- Detection of salmonella species in foodstuffs / Arvind A. Bhagwat ... [et al.] -- Identification of homozygous transgenic mice by genomic real-time PCR / Gunter Schmidtke and Marcus Groettrup -- Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCycler instrument and a SYBR greenI-based assay : determining FAT10 mRNA levels in hepatocellular carcinoma / Sebastian Lukasiak ... [et al.] -- Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR / Valentino Cattori and Regina Hofmann-Lehmann -- High-throughput real-time PCR / Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang -- Design and use of scorpions fluorescent signalling molecules / Rachael Carters ... [et al.] -- Hybridization probe pairs and single-labeled probes : an alternative approach for genotyping and quantification / Thomas Froehlich and Oliver Geulen -- EasyBeacons for the detection of methylation status of single CpG duplets / Ulf B. Christensen -- Quenched autoligation probes / Adam P. Silverman, Hiroshi Abe, and Eric T. Kool -- HyBeacon probes for rapid DNA sequence detection and allele discrimination / David J. French ... [et al.] -- FIT-probes in real-time PCR / Elke Socher and Oliver Seitz -- SNP genotyping by unlabeled probe melting analysis / Maria Erali, Robert Palais, and Carl Wittwer -- Molecular beacons for protein-DNA interaction studies / Jun Li ... [et al.] -- DNA polymerase profiling / D. Summerer -- Modular reporter hairpin ribozymes for analyzing molecular interactions / S. Hani Najafi-Shoushtari and Michael Famulok -- Screening of molecular interactions using reporter hammerhead ribozymes / Jörg S. Hartig and Michael Famulok |
| Beschreibung: | XII, 266 S. Ill., graph. Darst. 24 cm |
| ISBN: | 9781588297006 9781603270403 |
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| 300 | |a XII, 266 S. |b Ill., graph. Darst. |c 24 cm | ||
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| 490 | 1 | |a Methods in molecular biology |v 429 | |
| 500 | |a Includes bibliographical references and index | ||
| 500 | |a Basic principles of fluorescence and energy transfer / Larry E. Morrison -- Multiplex detection of mutations / David S. Perlin, Sergey Balashov, and Steven Park -- Detection of salmonella species in foodstuffs / Arvind A. Bhagwat ... [et al.] -- Identification of homozygous transgenic mice by genomic real-time PCR / Gunter Schmidtke and Marcus Groettrup -- Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCycler instrument and a SYBR greenI-based assay : determining FAT10 mRNA levels in hepatocellular carcinoma / Sebastian Lukasiak ... [et al.] -- Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR / Valentino Cattori and Regina Hofmann-Lehmann -- High-throughput real-time PCR / Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang -- Design and use of scorpions fluorescent signalling molecules / Rachael Carters ... [et al.] -- Hybridization probe pairs and single-labeled probes : an alternative approach for genotyping and quantification / Thomas Froehlich and Oliver Geulen -- EasyBeacons for the detection of methylation status of single CpG duplets / Ulf B. Christensen -- Quenched autoligation probes / Adam P. Silverman, Hiroshi Abe, and Eric T. Kool -- HyBeacon probes for rapid DNA sequence detection and allele discrimination / David J. French ... [et al.] -- FIT-probes in real-time PCR / Elke Socher and Oliver Seitz -- SNP genotyping by unlabeled probe melting analysis / Maria Erali, Robert Palais, and Carl Wittwer -- Molecular beacons for protein-DNA interaction studies / Jun Li ... [et al.] -- DNA polymerase profiling / D. Summerer -- Modular reporter hairpin ribozymes for analyzing molecular interactions / S. Hani Najafi-Shoushtari and Michael Famulok -- Screening of molecular interactions using reporter hammerhead ribozymes / Jörg S. Hartig and Michael Famulok | ||
| 520 | 3 | |a This edited work intends to show a diverse set of instructive examples, from probe design to applications in clinical settings, guided by experts in the field who provide easy-to-follow experimentals. | |
| 650 | 4 | |a Molecular biology | |
| 650 | 4 | |a Fluorescent probes | |
| 650 | 4 | |a Cellular signal transduction | |
| 650 | 4 | |a Molecular Probe Techniques / Laboratory Manuals | |
| 650 | 4 | |a Nucleic Acid Probes / Laboratory Manuals | |
| 650 | 4 | |a Cellular signal transduction | |
| 650 | 4 | |a Fluorescent probes | |
| 650 | 4 | |a Molecular Probe Techniques |v Laboratory Manuals | |
| 650 | 4 | |a Molecular biology | |
| 650 | 4 | |a Nucleic Acid Probes |v Laboratory Manuals | |
| 700 | 1 | |a Marx, Andreas |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 429 |w (DE-604)BV035362695 |9 429 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-016490544 | ||
Datensatz im Suchindex
| _version_ | 1804137637574868992 |
|---|---|
| adam_text | CONTENTS PREFACE V CONTRIBUTORS XI I. INTRODUCTION 1. BASIC PRINCIPLES
OF FLUORESCENCE AND ENERGY TRANSFER 3 LARRY E. MORRISON 11. APPLICATIONS
2. MULTIPLEX DETECTION OF MUTATIONS 23 DAVID S. PERLIN, SERGEY BALASHOV,
AND STEVEN PARK 3. DETECTION OF SALMONELLA SPECIES IN FOODSTUFFS 33
ARVIND A. BHAGWAT, LITU PETEL, TRINA CHUA, AUDREY CHAN, SAUL RUIZ CRUZ,
AND GUSTAVO A. GONZTILEZ AGUILAR 4. IDENTIFICATION OF HOMOZYGOUS
TRANSGENIE MICE BY GENOMIC REAL-TIME PCR 45 GUNTER SCHMIDTKE AND MARCUS
GROETTRUP 5. QUANTITATIVE ANALYSIS OF GENE EXPRESSION RELATIVE TO 18S
RRNA IN CARCINOMA SAMPIES USING THE LIGHTF.YCLER INSTRUMENT AND A SYBR
GREENI-BASED ASSAY: DETERMINING FATI0 MRNA LEVELS IN HEPATOCELLULAR
CARCINOMA 59 SEBASTIAN LUKASIAK, KAI BREUHAHN, CLAUDIA SCHILLER, GUNTER
SCHMIDTKE, AND MARCUS GROETTRUP 6. ABSOLUTE QUANTITATION OF FELINE
LEUKEMIA VIRUS PROVIRAL DNA AND VIRAL RNA LOADS BY TAQLVLAN REAL-TIME
PCR AND RT-PCR 73 VALENTINO CATTORI AND REGINA HOFMANN-LEHMANN 7.
HIGH-THROUGHPUT REAL-TIME PCR 89 THOMAS D. SCHMITTGEN, FUN IOO LEE, AND
LINMEI HANG 8. DESIGN AND USE OF SCORPIONS FLUORESCENT SIGNALLING
MOLEEULES 99 RACHAEL CARTERS, JENNIFER FERGUSON, RUPERT GAUT, PAUL
RAVETTO, NICOLA THELWELL, AND DAVID WHITCOMBE IX IV. NON-NUCLEIC ACID
TARGETS 15. MOLECULAR BEACONS FOR PROTEIN-DNA INTERACTION STUDIES .. 209
]UN LI, ZEHUI CHARLES CAO, ZHIWEN TANG, KEMIN WANG, AND WEIHONG TAN 16.
DNA POLYMERASE PROFILING 225 D. SUMMERER 17. MODULAR REPORTER HAIRPIN
RIBOZYMES FOR ANALYZING MOLECULAR INTERACTIONS 237 S. HANI
NAJAFI-SHOUSHTARI AND MICHAEL FAMU/OK 18. SCREENING OF MOLECULAR
INTERACTIONS USING REPORTER HAMMERHEAD RIBOZYMES 251 ]OERG S. HARTIG AND
MICHAEL FAMULOK INDEX 265 111. NEW TECHNOLOGIES 10. EASYSSEACONS FOR THE
DETECTION OF METHYLATION STATUS OF SINGLE CPG DUPLETS 137 VIF B.
CHRISTENSEN 11. QUENCHED AUTOLIGATION PROBES 161 ADAM P. SI/VERMAN,
HIROSHI ABE, AND FRIC T. KOOL 12. HYBEACON PROBES FOR RAPID DNA
SEQUENCE DETECTION AND ALLELE DISCRIMINATION 171 DAVID L. FRENCH, DAVID
G. MCDOWEJL, PAUL DEBENHAM, NITTAYA GALE, AND TOM BROWN 13. FIT-PROBES
IN REAL-TIME PER 187 ELKE SOCHER AND OLIVER SEITZ 14. SNP GENOTYPING BY
UNLABELED PROBE MELTING ANALYSIS ..... 199 MARIA FRA/I, ROBERT PALAIS,
AND CARL WITTWER X 9. CONTENTS HYBRIDIZATION PROBE PAIRS AND
SINGLE-LABELED PROBES: AN ALTERNATIVE APPROACH FOR GENOTYPING AND
QUANTIFICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . 117 THOMAS FROEHLICH AND OLIVER GEULEN
|
| adam_txt |
CONTENTS PREFACE V CONTRIBUTORS XI I. INTRODUCTION 1. BASIC PRINCIPLES
OF FLUORESCENCE AND ENERGY TRANSFER 3 LARRY E. MORRISON 11. APPLICATIONS
2. MULTIPLEX DETECTION OF MUTATIONS 23 DAVID S. PERLIN, SERGEY BALASHOV,
AND STEVEN PARK 3. DETECTION OF SALMONELLA SPECIES IN FOODSTUFFS 33
ARVIND A. BHAGWAT, LITU PETEL, TRINA CHUA, AUDREY CHAN, SAUL RUIZ CRUZ,
AND GUSTAVO A. GONZTILEZ AGUILAR 4. IDENTIFICATION OF HOMOZYGOUS
TRANSGENIE MICE BY GENOMIC REAL-TIME PCR 45 GUNTER SCHMIDTKE AND MARCUS
GROETTRUP 5. QUANTITATIVE ANALYSIS OF GENE EXPRESSION RELATIVE TO 18S
RRNA IN CARCINOMA SAMPIES USING THE LIGHTF.YCLER'" INSTRUMENT AND A SYBR
GREENI-BASED ASSAY: DETERMINING FATI0 MRNA LEVELS IN HEPATOCELLULAR
CARCINOMA 59 SEBASTIAN LUKASIAK, KAI BREUHAHN, CLAUDIA SCHILLER, GUNTER
SCHMIDTKE, AND MARCUS GROETTRUP 6. ABSOLUTE QUANTITATION OF FELINE
LEUKEMIA VIRUS PROVIRAL DNA AND VIRAL RNA LOADS BY TAQLVLAN" REAL-TIME
PCR AND RT-PCR 73 VALENTINO CATTORI AND REGINA HOFMANN-LEHMANN 7.
HIGH-THROUGHPUT REAL-TIME PCR 89 THOMAS D. SCHMITTGEN, FUN IOO LEE, AND
LINMEI HANG 8. DESIGN AND USE OF SCORPIONS FLUORESCENT SIGNALLING
MOLEEULES 99 RACHAEL CARTERS, JENNIFER FERGUSON, RUPERT GAUT, PAUL
RAVETTO, NICOLA THELWELL, AND DAVID WHITCOMBE IX IV. NON-NUCLEIC ACID
TARGETS 15. MOLECULAR BEACONS FOR PROTEIN-DNA INTERACTION STUDIES . 209
]UN LI, ZEHUI CHARLES CAO, ZHIWEN TANG, KEMIN WANG, AND WEIHONG TAN 16.
DNA POLYMERASE PROFILING 225 D. SUMMERER 17. MODULAR REPORTER HAIRPIN
RIBOZYMES FOR ANALYZING MOLECULAR INTERACTIONS 237 S. HANI
NAJAFI-SHOUSHTARI AND MICHAEL FAMU/OK 18. SCREENING OF MOLECULAR
INTERACTIONS USING REPORTER HAMMERHEAD RIBOZYMES 251 ]OERG S. HARTIG AND
MICHAEL FAMULOK INDEX 265 111. NEW TECHNOLOGIES 10. EASYSSEACONS" FOR THE
DETECTION OF METHYLATION STATUS OF SINGLE CPG DUPLETS 137 VIF B.
CHRISTENSEN 11. QUENCHED AUTOLIGATION PROBES 161 ADAM P. SI/VERMAN,
HIROSHI ABE, AND FRIC T. KOOL 12. HYBEACON PROBES FOR RAPID DNA
SEQUENCE DETECTION AND ALLELE DISCRIMINATION 171 DAVID L. FRENCH, DAVID
G. MCDOWEJL, PAUL DEBENHAM, NITTAYA GALE, AND TOM BROWN 13. FIT-PROBES
IN REAL-TIME PER 187 ELKE SOCHER AND OLIVER SEITZ 14. SNP GENOTYPING BY
UNLABELED PROBE MELTING ANALYSIS . 199 MARIA FRA/I, ROBERT PALAIS,
AND CARL WITTWER X 9. CONTENTS HYBRIDIZATION PROBE PAIRS AND
SINGLE-LABELED PROBES: AN ALTERNATIVE APPROACH FOR GENOTYPING AND
QUANTIFICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . 117 THOMAS FROEHLICH AND OLIVER GEULEN |
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| illustrated | Illustrated |
| index_date | 2024-07-02T20:48:35Z |
| indexdate | 2024-07-09T21:15:28Z |
| institution | BVB |
| isbn | 9781588297006 9781603270403 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016490544 |
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| physical | XII, 266 S. Ill., graph. Darst. 24 cm |
| publishDate | 2008 |
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| publisher | Humana |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Molecular beacons signalling nucleic acid probes, methods, and protocols ed. by Andreas Marx ... Totowa, N.J. Humana 2008 XII, 266 S. Ill., graph. Darst. 24 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 429 Includes bibliographical references and index Basic principles of fluorescence and energy transfer / Larry E. Morrison -- Multiplex detection of mutations / David S. Perlin, Sergey Balashov, and Steven Park -- Detection of salmonella species in foodstuffs / Arvind A. Bhagwat ... [et al.] -- Identification of homozygous transgenic mice by genomic real-time PCR / Gunter Schmidtke and Marcus Groettrup -- Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCycler instrument and a SYBR greenI-based assay : determining FAT10 mRNA levels in hepatocellular carcinoma / Sebastian Lukasiak ... [et al.] -- Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR / Valentino Cattori and Regina Hofmann-Lehmann -- High-throughput real-time PCR / Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang -- Design and use of scorpions fluorescent signalling molecules / Rachael Carters ... [et al.] -- Hybridization probe pairs and single-labeled probes : an alternative approach for genotyping and quantification / Thomas Froehlich and Oliver Geulen -- EasyBeacons for the detection of methylation status of single CpG duplets / Ulf B. Christensen -- Quenched autoligation probes / Adam P. Silverman, Hiroshi Abe, and Eric T. Kool -- HyBeacon probes for rapid DNA sequence detection and allele discrimination / David J. French ... [et al.] -- FIT-probes in real-time PCR / Elke Socher and Oliver Seitz -- SNP genotyping by unlabeled probe melting analysis / Maria Erali, Robert Palais, and Carl Wittwer -- Molecular beacons for protein-DNA interaction studies / Jun Li ... [et al.] -- DNA polymerase profiling / D. Summerer -- Modular reporter hairpin ribozymes for analyzing molecular interactions / S. Hani Najafi-Shoushtari and Michael Famulok -- Screening of molecular interactions using reporter hammerhead ribozymes / Jörg S. Hartig and Michael Famulok This edited work intends to show a diverse set of instructive examples, from probe design to applications in clinical settings, guided by experts in the field who provide easy-to-follow experimentals. Molecular biology Fluorescent probes Cellular signal transduction Molecular Probe Techniques / Laboratory Manuals Nucleic Acid Probes / Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Probes Laboratory Manuals Marx, Andreas Sonstige oth Methods in molecular biology 429 (DE-604)BV035362695 429 OEBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016490544&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molecular beacons signalling nucleic acid probes, methods, and protocols Methods in molecular biology Molecular biology Fluorescent probes Cellular signal transduction Molecular Probe Techniques / Laboratory Manuals Nucleic Acid Probes / Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Probes Laboratory Manuals |
| title | Molecular beacons signalling nucleic acid probes, methods, and protocols |
| title_auth | Molecular beacons signalling nucleic acid probes, methods, and protocols |
| title_exact_search | Molecular beacons signalling nucleic acid probes, methods, and protocols |
| title_exact_search_txtP | Molecular beacons signalling nucleic acid probes, methods, and protocols |
| title_full | Molecular beacons signalling nucleic acid probes, methods, and protocols ed. by Andreas Marx ... |
| title_fullStr | Molecular beacons signalling nucleic acid probes, methods, and protocols ed. by Andreas Marx ... |
| title_full_unstemmed | Molecular beacons signalling nucleic acid probes, methods, and protocols ed. by Andreas Marx ... |
| title_short | Molecular beacons |
| title_sort | molecular beacons signalling nucleic acid probes methods and protocols |
| title_sub | signalling nucleic acid probes, methods, and protocols |
| topic | Molecular biology Fluorescent probes Cellular signal transduction Molecular Probe Techniques / Laboratory Manuals Nucleic Acid Probes / Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Probes Laboratory Manuals |
| topic_facet | Molecular biology Fluorescent probes Cellular signal transduction Molecular Probe Techniques / Laboratory Manuals Nucleic Acid Probes / Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Probes Laboratory Manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016490544&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT marxandreas molecularbeaconssignallingnucleicacidprobesmethodsandprotocols |