Holdings: Enzyme kinetics :: THWS Bibkatalog

Enzyme kinetics: principles and methods

Saved in:

Bibliographic Details
Main Author:	Bisswanger, Hans 1943- (Author)
Format:	Book
Language:	English German
Published:	Weinheim WILEY-VCH 2008
Edition:	2., rev. and updated ed.
Subjects:	Cinétique enzymatique Cinétique enzymatique - Méthodologie Enzyme kinetics Enzymes > metabolism Kinetics Methode Enzymkinetik
Online Access:	Inhaltsverzeichnis Inhaltstext Inhaltsverzeichnis
Item Description:	Früher mit der Nummer 9783527303434
Physical Description:	XVIII, 301 S. graph. Darst. 240 mm x 170 mm
ISBN:	9783527319572 3527319573

Staff View

MARC


LEADER	00000nam a2200000 c 4500
001	BV023128164
003	DE-604
005	20080502
007	t
008	080213s2008 gw d\|\|\| \|\|\|\| 00\|\|\| eng d
015			\|a 07,N47,0984 \|2 dnb
016	7		\|a 986185752 \|2 DE-101
020			\|a 9783527319572 \|c Gb. : ca. EUR 139.00 (freier Pr.), ca. sfr 220.00 (freier Pr.) \|9 978-3-527-31957-2
020			\|a 3527319573 \|c Gb. : ca. EUR 139.00 (freier Pr.), ca. sfr 220.00 (freier Pr.) \|9 3-527-31957-3
024	3		\|a 9783527319572
028	5	2	\|a 1131957 000
035			\|a (OCoLC)225406378
035			\|a (DE-599)DNB986185752
040			\|a DE-604 \|b ger \|e rakddb
041	1		\|a eng \|h ger
044			\|a gw \|c XA-DE-BW
049			\|a DE-91G \|a DE-20 \|a DE-355 \|a DE-703 \|a DE-634 \|a DE-83 \|a DE-91S \|a DE-11 \|a DE-M49
050		0	\|a QP601.3
082	0		\|a 572/.744 \|2 22
084			\|a WC 4350 \|0 (DE-625)148095: \|2 rvk
084			\|a WD 5050 \|0 (DE-625)148185: \|2 rvk
084			\|a CHE 827f \|2 stub
084			\|a 570 \|2 sdnb
100	1		\|a Bisswanger, Hans \|d 1943- \|e Verfasser \|0 (DE-588)129060461 \|4 aut
245	1	0	\|a Enzyme kinetics \|b principles and methods \|c Hans Bisswanger
250			\|a 2., rev. and updated ed.
264		1	\|a Weinheim \|b WILEY-VCH \|c 2008
300			\|a XVIII, 301 S. \|b graph. Darst. \|c 240 mm x 170 mm
336			\|b txt \|2 rdacontent
337			\|b n \|2 rdamedia
338			\|b nc \|2 rdacarrier
500			\|a Früher mit der Nummer 9783527303434
650		4	\|a Cinétique enzymatique
650		4	\|a Cinétique enzymatique - Méthodologie
650		4	\|a Enzyme kinetics
650		4	\|a Enzymes \|x metabolism
650		4	\|a Kinetics
650	0	7	\|a Methode \|0 (DE-588)4038971-6 \|2 gnd \|9 rswk-swf
650	0	7	\|a Enzymkinetik \|0 (DE-588)4133166-7 \|2 gnd \|9 rswk-swf
689	0	0	\|a Enzymkinetik \|0 (DE-588)4133166-7 \|D s
689	0	1	\|a Methode \|0 (DE-588)4038971-6 \|D s
689	0		\|8 1\p \|5 DE-604
856	4		\|u http://d-nb.info/986185752/04 \|3 Inhaltsverzeichnis
856	4	2	\|q text/html \|u http://deposit.dnb.de/cgi-bin/dokserv?id=3018887&prov=M&dok_var=1&dok_ext=htm \|3 Inhaltstext
856	4	2	\|m Digitalisierung UB Regensburg \|q application/pdf \|u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016330545&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA \|3 Inhaltsverzeichnis
883	1		\|8 1\p \|a cgwrk \|d 20201028 \|q DE-101 \|u https://d-nb.info/provenance/plan#cgwrk
943	1		\|a oai:aleph.bib-bvb.de:BVB01-016330545

Record in the Search Index

_version_	1805089985514176512
adam_text	XI Contents Preface to the Second English Edition VII Prefece to the First English Edition IX Symbols and Abbreviations XVII Introduction and Definitions 1 References 4 1 Multiple Equilibria 7 1.1 Diffusion 8 1.2 Interaction between Macromolecules and Ligands 12 1.2.1 Binding Constants 12 1.2.2 Macromolecules with One Binding Site 13 1.3 Macromolecules with Identical Independent Binding Sites 14 1.3.1 General Binding Equation 14 1.3.2 Graphic Representations of the Binding Equation 20 1.3.2.1 Direct and Linear Diagrams 20 1.3.2.2 Analysis of Binding Data from Spectroscopie Titrations 22 1.3.3 Binding of Different Ligands, Competition 25 1.3.4 Non-competitive Binding 27 1.4 Macromolecules with Non-identical, Independent Binding Sites 29 1.5 Macromolecules with Identical, Interacting Binding Sites, Cooperativity 32 1.5.1 The Hill Equation 32 1.5.2 The Adair Equation 34 1.5.3 The Pauling Model 37 1.5.4 Allosteric Enzymes 38 1.5.5 The Symmetry or Concerted Model 39 1.5.6 The Sequential Model and Negative Cooperativity 44 1.5.7 Analysis of Cooperativity 48 1.5.8 Physiological Aspects of Cooperativity 50 1.5.9 Examples of Allosteric Enzymes 52 Enzyme Kinetics. Principles and Methods. 2nå Ш. Hans Bisswanger Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ISBN: 978-3-527-31957-2 XII Contents 1.5.9.1 Hemoglobin 52 1.5.9.2 Aspartate Transcarbamoylase 53 1.5.9.3 Aspartokinase 54 1.5.9.4 Phosphofractokinase 55 1.5.9.5 Allosteric Regulation of the Glycogen Metabolism 55 1.5.9.6 Membrane Bound Enzymes and Receptors 55 1.6 Non-identical, Interacting Binding Sites 56 References 57 2 Enzyme Kinetics 59 2.1 Reaction Order 59 2.1.1 First Order Reactions 60 2.1.2 Second Order Reactions 61 2.1.3 Zero Order Reactions 62 2.2 Steady-State Kinetics and the Michaelis-Menten Equation 63 2.2.1 Derivation of the Michaelis-Menten Equation 63 2.3 Analysis of Enzyme Kinetic Data 66 2.3.1 Graphical Representations of the Michaelis-Menten Equation 66 2.3.1.1 Direct and Semi-logarithmic Representations 66 2.3.1.2 Direct Linear Plots 73 2.3.1.3 Linearization Methods 75 2.3.2 Analysis of Progress Curves 77 2.3.2.1 Integrated Michaelis-Menten Equation 78 2.3.2.2 Determination of Reaction Rates 80 2.3.2.3 Graphic Methods for Rate Determination 82 2.3.2.4 Graphic Determination of True Initial Rates 84 2.4 Reversible Enzyme Reactions 85 2.4.1 Rate Equation for Reversible Enzyme Reactions 85 2.4.2 The Haldane Relationship 87 2.4.3 Product Inhibition 88 2.5 Enzyme Inhibition 91 2.5.1 Unspecific Enzyme Inhibition 91 2.5.2 Irreversible Enzyme Inhibition 92 2.5.2.1 General Features of Irreversible Enzyme Inhibition 92 2.5.2.2 Suicide Substrates 93 2.5.2.3 Transition State Analogs 95 2.5.2.4 Analysis of Irreversible Inhibitions 96 2.5.3 Reversible Enzyme Inhibition 98 2.5.3.1 General Rate Equation 98 2.5.3.2 Non-Competitive Inhibition and Graphic Representation of Inhibition Data 101 2.5.3.3 Competitive Inhibition 107 2.5.3.4 Uncompetitive Inhibition 111 2.5.3.5 Partially Non-competitive Inhibition 113 Contents XIII 2.5.3.6 Partially Uncompetitive Inhibition 115 2.5.3.7 Partially Competitive Inhibition 117 2.5.3.8 Noncompetitive and Uncompetitive Product Inhibition 119 2.5.3.9 Substrate Inhibition 120 2.5.4 Enzyme Reactions with Two Competing Substrates 121 2.5.5 Different Enzymes Catalyzing the Same Reaction 123 2.6 Multi-substrate Reactions 124 2.6.1 Nomenclature 124 2. ii. 2 Random Mechanism 126 2.6.3 Ordered Mechanism 131 2.6.4 Ping-pong Mechanism 132 2.6.5 Product Inhibition in Multi-substrate Reactions 135 2.6.6 Haldane Relationships in Multi-substrate Reactions 135 2.6.7 Mechanisms with more than Two Substrates 136 2.6.8 Other Nomenclatures for Multi-substrate Reactions 138 2.7 Derivation of Rate Equations of Complex Enzyme Mechanisms 138 2.7.1 King-Altmann Method 138 2.7.2 Simplified Derivations Applying Graph Theory 144 2.7.3 Combination of Equilibrium and Steady State Approach 145 2.8 Kinetic Treatment of Allosteric Enzymes 147 2.8.1 Hysteretic Enzymes 148 2.8.2 Kinetic Cooperativity, the Slow Transition Model 249 2.9 pH and Temperature Dependence of Enzymes 151 2.9.1 pH Optimum and Determination of pK Values 151 2.9.2 pH Stability 153 2.9.3 Temperature Dependence 154 2.10 Isotope Exchange 158 2.10.1 Isotope Exchange Kinetics 159 2.10.2 Isotope Effects 163 2.10.2.1 Primary Kinetic Isotope Effect 163 2.10.2.2 Influence of the Kinetic Isotope Effect on V and Кш 164 2.10.2.3 Other Isotope Effects 165 2.11 Special Enzyme Mechanisms 166 2.11.1 Ribozymes 166 2.11.2 Polymer Substrates 167 2.11.3 Kinetics of Immobilized Enzymes 168 2.11.3.1 External Diffusion Limitation 169 2.11.3.2 Internal Diffusion limitation 172 2.11.3.3 Inhibition of Immobilized Enzymes 173 2.11.3.4 pH and Temperature Behavior of Immobilized Enzymes 174 2.11.4 Transport Processes 175 2.11.5 Enzyme Reactions at Membrane Interfaces 178 2.12 Application of Statistical Methods in Enzyme Kinetics 185 2.12.1 General Remarks 185 XIV I Contents 2.12.2 Statistical Terms Used in Enzyme Kinetics 189 References 190 3 Methods 195 3.1 Methods for Investigation of Multiple Equilibria 195 3.1.1 Equilibrium Dialysis and General Aspects of Binding Measurements 197 3.1.1.1 Equilibrium Dialysis 197 3.1.1.2 Control Experiments and Sources of Error 200 3.1.1.3 Continuous Equilibrium Dialysis 203 3.1.2 Ultrafiltration 206 3.1.3 Gel Filtration 207 3.1.3.1 Batch Method 208 3.1.3.2 The Method of Hummel and Dreyer 209 3.1.3.3 Other Gel Filtration Methods 210 3.1.4 Ultracentrifugation 211 3.1.4.1 Fixed Angle Ultracentrifugation Methods 212 3.1.4.2 Sucrose Gradient Centrifugation 214 3.1.5 Surface Plasmon Resonance 218 3.2 Electrochemical Methods 219 3.2.1 The Oxygen Electrode 220 3.2.2 The CO2 Electrode 222 3.2.3 Potentiometry, Redox Potentials 223 3.2.4 The pH-stat 223 3.2.5 Polarography 225 3.3 Calorimetry 226 3.4 Spectroscopie Methods 228 3.4.1 Absorption Spectroscopy 230 3.4.1.1 The Lambert-Beer Law 230 3.4.1.2 Spectral Properties of Enzymes and Ligands 231 3.4.1.3 Structure of Spectrophotometers 235 3.4.1.4 Double Beam Spectrophotometer 237 3.4.1.5 Difference Spectroscopy 238 3.4.1.6 The Dual Wavelength Spectrophotometer 241 3.4.1.7 Photochemical Action Spectra 242 3.4.2 Bioluminescence 243 3.4.3 Fluorescence 243 3.4.3.1 Quantum Yield 243 3.4.3.2 Structure of Spectrofluorimeters 244 3.4.3.3 Perturbations of Fluorescence Measurements 246 3.4.3.4 Fluorescent Compounds (Fluorophores) 247 3.4.3.5 Radiationless Energy Transfer 252 3.4.3.6 Fluorescence Polarization 254 3.4.3.7 Pulse Fluorimetry 255 Contents XV 3.4.4 Circular Dichroism and Optical Rotation Dispersion 257 3.4.5 Infrared and Raman Spectroscopy 262 3.4.5.1 IR Spectroscopy 263 3.4.5.2 Raman Spectroscopy 263 3.4.5.3 Applications 264 3.4.6 Electron Paramagnetic Resonance Spectroscopy 264 3.5 Measurement of Fast Reactions 267 3.5.1 Flow Methods 268 3.5.1.1 The Continuous Flow Method 268 3.5.1.2 The Stopped-flow Method 273 3.5.1.3 Measurement of Enzyme Reactions by Flow Methods 274 3.5.1.4 Determination of the Dead Time 276 3.5.2 Relaxation Methods 277 3.5.2.1 The Temperature Jump Method 278 3.5.2.2 The Pressure Jump Method 281 3.5.2.3 The Electric Field Method 283 3.5.3 Flash Photolysis, Pico- and Femto-second Spectroscopy 283 3.5.4 Evaluation of Rapid Kinetic Reactions (Transient Kinetics) 285 References 289 Subject Index 293
adam_txt	XI Contents Preface to the Second English Edition VII Prefece to the First English Edition IX Symbols and Abbreviations XVII Introduction and Definitions 1 References 4 1 Multiple Equilibria 7 1.1 Diffusion 8 1.2 Interaction between Macromolecules and Ligands 12 1.2.1 Binding Constants 12 1.2.2 Macromolecules with One Binding Site 13 1.3 Macromolecules with Identical Independent Binding Sites 14 1.3.1 General Binding Equation 14 1.3.2 Graphic Representations of the Binding Equation 20 1.3.2.1 Direct and Linear Diagrams 20 1.3.2.2 Analysis of Binding Data from Spectroscopie Titrations 22 1.3.3 Binding of Different Ligands, Competition 25 1.3.4 Non-competitive Binding 27 1.4 Macromolecules with Non-identical, Independent Binding Sites 29 1.5 Macromolecules with Identical, Interacting Binding Sites, Cooperativity 32 1.5.1 The Hill Equation 32 1.5.2 The Adair Equation 34 1.5.3 The Pauling Model 37 1.5.4 Allosteric Enzymes 38 1.5.5 The Symmetry or Concerted Model 39 1.5.6 The Sequential Model and Negative Cooperativity 44 1.5.7 Analysis of Cooperativity 48 1.5.8 Physiological Aspects of Cooperativity 50 1.5.9 Examples of Allosteric Enzymes 52 Enzyme Kinetics. Principles and Methods. 2nå Ш. Hans Bisswanger Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ISBN: 978-3-527-31957-2 XII Contents 1.5.9.1 Hemoglobin 52 1.5.9.2 Aspartate Transcarbamoylase 53 1.5.9.3 Aspartokinase 54 1.5.9.4 Phosphofractokinase 55 1.5.9.5 Allosteric Regulation of the Glycogen Metabolism 55 1.5.9.6 Membrane Bound Enzymes and Receptors 55 1.6 Non-identical, Interacting Binding Sites 56 References 57 2 Enzyme Kinetics 59 2.1 Reaction Order 59 2.1.1 First Order Reactions 60 2.1.2 Second Order Reactions 61 2.1.3 Zero Order Reactions 62 2.2 Steady-State Kinetics and the Michaelis-Menten Equation 63 2.2.1 Derivation of the Michaelis-Menten Equation 63 2.3 Analysis of Enzyme Kinetic Data 66 2.3.1 Graphical Representations of the Michaelis-Menten Equation 66 2.3.1.1 Direct and Semi-logarithmic Representations 66 2.3.1.2 Direct Linear Plots 73 2.3.1.3 Linearization Methods 75 2.3.2 Analysis of Progress Curves 77 2.3.2.1 Integrated Michaelis-Menten Equation 78 2.3.2.2 Determination of Reaction Rates 80 2.3.2.3 Graphic Methods for Rate Determination 82 2.3.2.4 Graphic Determination of True Initial Rates 84 2.4 Reversible Enzyme Reactions 85 2.4.1 Rate Equation for Reversible Enzyme Reactions 85 2.4.2 The Haldane Relationship 87 2.4.3 Product Inhibition 88 2.5 Enzyme Inhibition 91 2.5.1 Unspecific Enzyme Inhibition 91 2.5.2 Irreversible Enzyme Inhibition 92 2.5.2.1 General Features of Irreversible Enzyme Inhibition 92 2.5.2.2 Suicide Substrates 93 2.5.2.3 Transition State Analogs 95 2.5.2.4 Analysis of Irreversible Inhibitions 96 2.5.3 Reversible Enzyme Inhibition 98 2.5.3.1 General Rate Equation 98 2.5.3.2 Non-Competitive Inhibition and Graphic Representation of Inhibition Data 101 2.5.3.3 Competitive Inhibition 107 2.5.3.4 Uncompetitive Inhibition 111 2.5.3.5 Partially Non-competitive Inhibition 113 Contents XIII 2.5.3.6 Partially Uncompetitive Inhibition 115 2.5.3.7 Partially Competitive Inhibition 117 2.5.3.8 Noncompetitive and Uncompetitive Product Inhibition 119 2.5.3.9 Substrate Inhibition 120 2.5.4 Enzyme Reactions with Two Competing Substrates 121 2.5.5 Different Enzymes Catalyzing the Same Reaction 123 2.6 Multi-substrate Reactions 124 2.6.1 Nomenclature 124 2. ii. 2 Random Mechanism 126 2.6.3 Ordered Mechanism 131 2.6.4 Ping-pong Mechanism 132 2.6.5 Product Inhibition in Multi-substrate Reactions 135 2.6.6 Haldane Relationships in Multi-substrate Reactions 135 2.6.7 Mechanisms with more than Two Substrates 136 2.6.8 Other Nomenclatures for Multi-substrate Reactions 138 2.7 Derivation of Rate Equations of Complex Enzyme Mechanisms 138 2.7.1 King-Altmann Method 138 2.7.2 Simplified Derivations Applying Graph Theory 144 2.7.3 Combination of Equilibrium and Steady State Approach 145 2.8 Kinetic Treatment of Allosteric Enzymes 147 2.8.1 Hysteretic Enzymes 148 2.8.2 Kinetic Cooperativity, the Slow Transition Model 249 2.9 pH and Temperature Dependence of Enzymes 151 2.9.1 pH Optimum and Determination of pK Values 151 2.9.2 pH Stability 153 2.9.3 Temperature Dependence 154 2.10 Isotope Exchange 158 2.10.1 Isotope Exchange Kinetics 159 2.10.2 Isotope Effects 163 2.10.2.1 Primary Kinetic Isotope Effect 163 2.10.2.2 Influence of the Kinetic Isotope Effect on V and Кш 164 2.10.2.3 Other Isotope Effects 165 2.11 Special Enzyme Mechanisms 166 2.11.1 Ribozymes 166 2.11.2 Polymer Substrates 167 2.11.3 Kinetics of Immobilized Enzymes 168 2.11.3.1 External Diffusion Limitation 169 2.11.3.2 Internal Diffusion limitation 172 2.11.3.3 Inhibition of Immobilized Enzymes 173 2.11.3.4 pH and Temperature Behavior of Immobilized Enzymes 174 2.11.4 Transport Processes 175 2.11.5 Enzyme Reactions at Membrane Interfaces 178 2.12 Application of Statistical Methods in Enzyme Kinetics 185 2.12.1 General Remarks 185 XIV I Contents 2.12.2 Statistical Terms Used in Enzyme Kinetics 189 References 190 3 Methods 195 3.1 Methods for Investigation of Multiple Equilibria 195 3.1.1 Equilibrium Dialysis and General Aspects of Binding Measurements 197 3.1.1.1 Equilibrium Dialysis 197 3.1.1.2 Control Experiments and Sources of Error 200 3.1.1.3 Continuous Equilibrium Dialysis 203 3.1.2 Ultrafiltration 206 3.1.3 Gel Filtration 207 3.1.3.1 Batch Method 208 3.1.3.2 The Method of Hummel and Dreyer 209 3.1.3.3 Other Gel Filtration Methods 210 3.1.4 Ultracentrifugation 211 3.1.4.1 Fixed Angle Ultracentrifugation Methods 212 3.1.4.2 Sucrose Gradient Centrifugation 214 3.1.5 Surface Plasmon Resonance 218 3.2 Electrochemical Methods 219 3.2.1 The Oxygen Electrode 220 3.2.2 The CO2 Electrode 222 3.2.3 Potentiometry, Redox Potentials 223 3.2.4 The pH-stat 223 3.2.5 Polarography 225 3.3 Calorimetry 226 3.4 Spectroscopie Methods 228 3.4.1 Absorption Spectroscopy 230 3.4.1.1 The Lambert-Beer Law 230 3.4.1.2 Spectral Properties of Enzymes and Ligands 231 3.4.1.3 Structure of Spectrophotometers 235 3.4.1.4 Double Beam Spectrophotometer 237 3.4.1.5 Difference Spectroscopy 238 3.4.1.6 The Dual Wavelength Spectrophotometer 241 3.4.1.7 Photochemical Action Spectra 242 3.4.2 Bioluminescence 243 3.4.3 Fluorescence 243 3.4.3.1 Quantum Yield 243 3.4.3.2 Structure of Spectrofluorimeters 244 3.4.3.3 Perturbations of Fluorescence Measurements 246 3.4.3.4 Fluorescent Compounds (Fluorophores) 247 3.4.3.5 Radiationless Energy Transfer 252 3.4.3.6 Fluorescence Polarization 254 3.4.3.7 Pulse Fluorimetry 255 Contents XV 3.4.4 Circular Dichroism and Optical Rotation Dispersion 257 3.4.5 Infrared and Raman Spectroscopy 262 3.4.5.1 IR Spectroscopy 263 3.4.5.2 Raman Spectroscopy 263 3.4.5.3 Applications 264 3.4.6 Electron Paramagnetic Resonance Spectroscopy 264 3.5 Measurement of Fast Reactions 267 3.5.1 Flow Methods 268 3.5.1.1 The Continuous Flow Method 268 3.5.1.2 The Stopped-flow Method 273 3.5.1.3 Measurement of Enzyme Reactions by Flow Methods 274 3.5.1.4 Determination of the Dead Time 276 3.5.2 Relaxation Methods 277 3.5.2.1 The Temperature Jump Method 278 3.5.2.2 The Pressure Jump Method 281 3.5.2.3 The Electric Field Method 283 3.5.3 Flash Photolysis, Pico- and Femto-second Spectroscopy 283 3.5.4 Evaluation of Rapid Kinetic Reactions (Transient Kinetics) 285 References 289 Subject Index 293
any_adam_object	1
any_adam_object_boolean	1
author	Bisswanger, Hans 1943-
author_GND	(DE-588)129060461
author_facet	Bisswanger, Hans 1943-
author_role	aut
author_sort	Bisswanger, Hans 1943-
author_variant	h b hb
building	Verbundindex
bvnumber	BV023128164
callnumber-first	Q - Science
callnumber-label	QP601
callnumber-raw	QP601.3
callnumber-search	QP601.3
callnumber-sort	QP 3601.3
callnumber-subject	QP - Physiology
classification_rvk	WC 4350 WD 5050
classification_tum	CHE 827f
ctrlnum	(OCoLC)225406378 (DE-599)DNB986185752
dewey-full	572/.744
dewey-hundreds	500 - Natural sciences and mathematics
dewey-ones	572 - Biochemistry
dewey-raw	572/.744
dewey-search	572/.744
dewey-sort	3572 3744
dewey-tens	570 - Biology
discipline	Biologie Chemie
discipline_str_mv	Biologie Chemie
edition	2., rev. and updated ed.
format	Book
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV023128164</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20080502</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">080213s2008 gw d\|\|\| \|\|\|\| 00\|\|\| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">07,N47,0984</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">986185752</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783527319572</subfield><subfield code="c">Gb. : ca. EUR 139.00 (freier Pr.), ca. sfr 220.00 (freier Pr.)</subfield><subfield code="9">978-3-527-31957-2</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">3527319573</subfield><subfield code="c">Gb. : ca. EUR 139.00 (freier Pr.), ca. sfr 220.00 (freier Pr.)</subfield><subfield code="9">3-527-31957-3</subfield></datafield><datafield tag="024" ind1="3" ind2=" "><subfield code="a">9783527319572</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">1131957 000</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)225406378</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB986185752</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="1" ind2=" "><subfield code="a">eng</subfield><subfield code="h">ger</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BW</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91G</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-634</subfield><subfield code="a">DE-83</subfield><subfield code="a">DE-91S</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-M49</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QP601.3</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572/.744</subfield><subfield code="2">22</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4350</subfield><subfield code="0">(DE-625)148095:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5050</subfield><subfield code="0">(DE-625)148185:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 827f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bisswanger, Hans</subfield><subfield code="d">1943-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)129060461</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enzyme kinetics</subfield><subfield code="b">principles and methods</subfield><subfield code="c">Hans Bisswanger</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">2., rev. and updated ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Weinheim</subfield><subfield code="b">WILEY-VCH</subfield><subfield code="c">2008</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XVIII, 301 S.</subfield><subfield code="b">graph. Darst.</subfield><subfield code="c">240 mm x 170 mm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Früher mit der Nummer 9783527303434</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cinétique enzymatique</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cinétique enzymatique - Méthodologie</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Enzyme kinetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Enzymes</subfield><subfield code="x">metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Kinetics</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Enzymkinetik</subfield><subfield code="0">(DE-588)4133166-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Enzymkinetik</subfield><subfield code="0">(DE-588)4133166-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://d-nb.info/986185752/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="q">text/html</subfield><subfield code="u">http://deposit.dnb.de/cgi-bin/dokserv?id=3018887&prov=M&dok_var=1&dok_ext=htm</subfield><subfield code="3">Inhaltstext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016330545&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-016330545</subfield></datafield></record></collection>
id	DE-604.BV023128164
illustrated	Illustrated
index_date	2024-07-02T19:54:14Z
indexdate	2024-07-20T09:32:37Z
institution	BVB
isbn	9783527319572 3527319573
language	English German
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-016330545
oclc_num	225406378
open_access_boolean
owner	DE-91G DE-BY-TUM DE-20 DE-355 DE-BY-UBR DE-703 DE-634 DE-83 DE-91S DE-BY-TUM DE-11 DE-M49 DE-BY-TUM
owner_facet	DE-91G DE-BY-TUM DE-20 DE-355 DE-BY-UBR DE-703 DE-634 DE-83 DE-91S DE-BY-TUM DE-11 DE-M49 DE-BY-TUM
physical	XVIII, 301 S. graph. Darst. 240 mm x 170 mm
publishDate	2008
publishDateSearch	2008
publishDateSort	2008
publisher	WILEY-VCH
record_format	marc
spelling	Bisswanger, Hans 1943- Verfasser (DE-588)129060461 aut Enzyme kinetics principles and methods Hans Bisswanger 2., rev. and updated ed. Weinheim WILEY-VCH 2008 XVIII, 301 S. graph. Darst. 240 mm x 170 mm txt rdacontent n rdamedia nc rdacarrier Früher mit der Nummer 9783527303434 Cinétique enzymatique Cinétique enzymatique - Méthodologie Enzyme kinetics Enzymes metabolism Kinetics Methode (DE-588)4038971-6 gnd rswk-swf Enzymkinetik (DE-588)4133166-7 gnd rswk-swf Enzymkinetik (DE-588)4133166-7 s Methode (DE-588)4038971-6 s 1\p DE-604 http://d-nb.info/986185752/04 Inhaltsverzeichnis text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3018887&prov=M&dok_var=1&dok_ext=htm Inhaltstext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016330545&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk
spellingShingle	Bisswanger, Hans 1943- Enzyme kinetics principles and methods Cinétique enzymatique Cinétique enzymatique - Méthodologie Enzyme kinetics Enzymes metabolism Kinetics Methode (DE-588)4038971-6 gnd Enzymkinetik (DE-588)4133166-7 gnd
subject_GND	(DE-588)4038971-6 (DE-588)4133166-7
title	Enzyme kinetics principles and methods
title_auth	Enzyme kinetics principles and methods
title_exact_search	Enzyme kinetics principles and methods
title_exact_search_txtP	Enzyme kinetics principles and methods
title_full	Enzyme kinetics principles and methods Hans Bisswanger
title_fullStr	Enzyme kinetics principles and methods Hans Bisswanger
title_full_unstemmed	Enzyme kinetics principles and methods Hans Bisswanger
title_short	Enzyme kinetics
title_sort	enzyme kinetics principles and methods
title_sub	principles and methods
topic	Cinétique enzymatique Cinétique enzymatique - Méthodologie Enzyme kinetics Enzymes metabolism Kinetics Methode (DE-588)4038971-6 gnd Enzymkinetik (DE-588)4133166-7 gnd
topic_facet	Cinétique enzymatique Cinétique enzymatique - Méthodologie Enzyme kinetics Enzymes metabolism Kinetics Methode Enzymkinetik
url	http://d-nb.info/986185752/04 http://deposit.dnb.de/cgi-bin/dokserv?id=3018887&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016330545&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT bisswangerhans enzymekineticsprinciplesandmethods

Holdings

There is no print copy available.

Interlibrary loan Place Request Caution: Not in THWS collection! Indexes
Description

MARC

Record in the Search Index

There is no print copy available.

Similar Items