Post-translational modifications of proteins: tools for functional proteomics
This volume provides protocols of methods used to detect and analyse co- and post-translational modifications of proteins. It enables the reader to analyse the most important post-translational modifications focusing on proteome research.
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
Ausgabe: | 2. ed. |
Schriftenreihe: | Methods in molecular biology
446 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Zusammenfassung: | This volume provides protocols of methods used to detect and analyse co- and post-translational modifications of proteins. It enables the reader to analyse the most important post-translational modifications focusing on proteome research. |
Beschreibung: | XVII, 390 S. Ill., graph. Darst. |
ISBN: | 9781588297198 9781603270847 1588297195 |
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490 | 1 | |a Methods in molecular biology |v 446 | |
520 | 3 | |a This volume provides protocols of methods used to detect and analyse co- and post-translational modifications of proteins. It enables the reader to analyse the most important post-translational modifications focusing on proteome research. | |
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650 | 4 | |a Protein Processing, Post-Translational |v Laboratory Manuals | |
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Datensatz im Suchindex
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adam_text | Contents
Preface
.......................................................
v
Contributors
.................................................. xiii
1
Disulfide
Bond Mapping by Cyanylation-induced Cleavage
and Mass Spectrometry
...................................... 1
Jiang Wu
2
Detection of Post-translational Modifications by Fluorescent
Staining of Two-Dimensional Gels
............................. 21
Archana
M.
Jacob and Chris
W. Turek
3
Identification of Protein Phosphorylation Sites by Advanced
LC-ESI-MS/MS Methods
......................................... 33
Christoph Weise
and
Christof Lenz
4
Analysis of Tyrosine-O-Sulfation
............................... 47
Jens R. Bundgaard,
Jette
W.
Sen, Anders H.
Johnsen,
and
Jens F. Rehfeld
5
ot-Amidated Peptides: Approaches for Analysis
................... 67
Gregory P. Mueller and William J. Driscoll
6
y-Glutamate and
ß-Hydroxyaspartate in
Proteins
................ 85
Francis J.
Castellino,
Victoria A.
Ploplis,
and Li Zhang
7
Lysine Hydroxylation
and Cross-linking of Collagen
............. 95
Mitsuo
Yamauchi and Masashi Shiiba
8
Mass Spectrometric Determination of Protein Ubiquitination
..... 109
Carol E. Parker, Maria R. E. Warren, Viorel Mocanu.
Susanna F. Greer. and
Christoph
H.
Borchers
x
Contents
9
Analysis of Sumoylation
.................................... 131
Andrea Pichler
10
Detection and Analysis of Protein ISGylation
................... 139
Tomoharu Takeuchi and Hideyoshi Yokosawa
11
Analysis of Methylation, Acetylation, and Other Modifications
in Bacterial Ribosomal Proteins
................................ 151
Randy J. Arnold, William Running, and James P. Reilly
12
Analysis of S-Acylation of Proteins
........................... 163
Michael
Veit,
Evgeni Ponimaskin, and Michael
F. G.
Schmidt
13
Metabolic Labeling and Structural Analysis
of Glycosylphosphatidylinositols from Parasitic Protozoa
........ 183
Nahid Azzouz, Peter
Gerold,
and Ralph T.
Schwarz
14
2-Dimensional Electrophoresis: Detection of Glycosylation
and Influence on Spot Pattern
............................. 199
Klemens
Löster
and
Christoph
Kannicht
15
Carbohydrate Composition Analysis of Glycoproteins
by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag
... 215
George
N.
Saddic, Shirish T. Dhume, and Kalyan R. Anumula
16
Enzymatical Hydrolysis of
.ŕV-Glycans
from Glycoproteins
and Fluorescent Labeling by 2-Aminobenzamide
(2-AB)......... 231
RolfNuck
17
Separation of N-Glycans by HPLC
........................... 239
Martin Gohlke and
Véronique Blanchard
18
Enzymatic Sequence Analysis of iV-Glycans by Exoglycosidase
Cleavage and Mass Spectrometry—detection
of Lewis X Structures
...................................... 255
Christoph
Kannicht,
Detlef Grunow,
and
Lothar
Lucka
19
Immunochemical Methods for the Rapid Screening
of the O-GIycosidically Linked JV-Acetylglucosamine
Modification of Proteins
.................................... 267
Michael Ahrend, Angela
Käberich, Marie-Therese Fergen,
and
Brigitte Schmitz
20
Analysis of O-Glycosylation
................................. 281
Juan J.
Calvete
and Libia Sanz
Contents xi
21 Characterization
of
Site-spedflc ¿V-Glycosylation............... 293
Katalin
F.
Medzihradszky
22 Monitoring Glycosylation
of Therapeutic Glycoproteins
for Consistency by HPLC Using Highly Fluorescent
Anthranilic Acid (AA) Tag
.................................. 317
Shirish T. Dhume, George
N.
Saddic, and Kalyan R. Anumula
23
Comparability and Monitoring Immunogenic AMinked
Oligosaccharides from
Recombinant
Monoclonal Antibodies
from Two Different Cell Lines using HPLC with Fluorescence
Detection and Mass Spectrometry
............................ 333
Brace R. Kilgore, Adam W.
Lucka,
Rekha
Patel,
Bruce A. Andrien, Jr., and Shirish T. Dhume
24
Mass Spectrometry and HPLC with Fluorescent Detection-Based
Orthogonal Approaches to Characterize ^-linked Oligosaccharides
of
Recombinant
Monoclonal Antibodies
....................... 347
Adam
W. Lucka,
Bruce R. Kilgore, Rekha
Patel,
Bruce A. Andrien, Jr.,
and Shirish T. Dhume
25
Web-based Computational Tools for the Prediction and Analysis
of Post-translational Modifications of Proteins
.................. 363
Vladimir A. Ivanisenko, Dmitry A. Afonnikov,
and Nikolay A. Kolchanov
Index
........................................................ 385
|
adam_txt |
Contents
Preface
.
v
Contributors
. xiii
1
Disulfide
Bond Mapping by Cyanylation-induced Cleavage
and Mass Spectrometry
. 1
Jiang Wu
2
Detection of Post-translational Modifications by Fluorescent
Staining of Two-Dimensional Gels
. 21
Archana
M.
Jacob and Chris
W. Turek
3
Identification of Protein Phosphorylation Sites by Advanced
LC-ESI-MS/MS Methods
. 33
Christoph Weise
and
Christof Lenz
4
Analysis of Tyrosine-O-Sulfation
. 47
Jens R. Bundgaard,
Jette
W.
Sen, Anders H.
Johnsen,
and
Jens F. Rehfeld
5
ot-Amidated Peptides: Approaches for Analysis
. 67
Gregory P. Mueller and William J. Driscoll
6
y-Glutamate and
ß-Hydroxyaspartate in
Proteins
. 85
Francis J.
Castellino,
Victoria A.
Ploplis,
and Li Zhang
7
Lysine Hydroxylation
and Cross-linking of Collagen
. 95
Mitsuo
Yamauchi and Masashi Shiiba
8
Mass Spectrometric Determination of Protein Ubiquitination
. 109
Carol E. Parker, Maria R. E. Warren, Viorel Mocanu.
Susanna F. Greer. and
Christoph
H.
Borchers
x
Contents
9
Analysis of Sumoylation
. 131
Andrea Pichler
10
Detection and Analysis of Protein ISGylation
. 139
Tomoharu Takeuchi and Hideyoshi Yokosawa
11
Analysis of Methylation, Acetylation, and Other Modifications
in Bacterial Ribosomal Proteins
. 151
Randy J. Arnold, William Running, and James P. Reilly
12
Analysis of S-Acylation of Proteins
. 163
Michael
Veit,
Evgeni Ponimaskin, and Michael
F. G.
Schmidt
13
Metabolic Labeling and Structural Analysis
of Glycosylphosphatidylinositols from Parasitic Protozoa
. 183
Nahid Azzouz, Peter
Gerold,
and Ralph T.
Schwarz
14
2-Dimensional Electrophoresis: Detection of Glycosylation
and Influence on Spot Pattern
. 199
Klemens
Löster
and
Christoph
Kannicht
15
Carbohydrate Composition Analysis of Glycoproteins
by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag
. 215
George
N.
Saddic, Shirish T. Dhume, and Kalyan R. Anumula
16
Enzymatical Hydrolysis of
.ŕV-Glycans
from Glycoproteins
and Fluorescent Labeling by 2-Aminobenzamide
(2-AB). 231
RolfNuck
17
Separation of N-Glycans by HPLC
. 239
Martin Gohlke and
Véronique Blanchard
18
Enzymatic Sequence Analysis of iV-Glycans by Exoglycosidase
Cleavage and Mass Spectrometry—detection
of Lewis X Structures
. 255
Christoph
Kannicht,
Detlef Grunow,
and
Lothar
Lucka
19
Immunochemical Methods for the Rapid Screening
of the O-GIycosidically Linked JV-Acetylglucosamine
Modification of Proteins
. 267
Michael Ahrend, Angela
Käberich, Marie-Therese Fergen,
and
Brigitte Schmitz
20
Analysis of O-Glycosylation
. 281
Juan J.
Calvete
and Libia Sanz
Contents xi
21 Characterization
of
Site-spedflc ¿V-Glycosylation. 293
Katalin
F.
Medzihradszky
22 Monitoring Glycosylation
of Therapeutic Glycoproteins
for Consistency by HPLC Using Highly Fluorescent
Anthranilic Acid (AA) Tag
. 317
Shirish T. Dhume, George
N.
Saddic, and Kalyan R. Anumula
23
Comparability and Monitoring Immunogenic AMinked
Oligosaccharides from
Recombinant
Monoclonal Antibodies
from Two Different Cell Lines using HPLC with Fluorescence
Detection and Mass Spectrometry
. 333
Brace R. Kilgore, Adam W.
Lucka,
Rekha
Patel,
Bruce A. Andrien, Jr., and Shirish T. Dhume
24
Mass Spectrometry and HPLC with Fluorescent Detection-Based
Orthogonal Approaches to Characterize ^-linked Oligosaccharides
of
Recombinant
Monoclonal Antibodies
. 347
Adam
W. Lucka,
Bruce R. Kilgore, Rekha
Patel,
Bruce A. Andrien, Jr.,
and Shirish T. Dhume
25
Web-based Computational Tools for the Prediction and Analysis
of Post-translational Modifications of Proteins
. 363
Vladimir A. Ivanisenko, Dmitry A. Afonnikov,
and Nikolay A. Kolchanov
Index
. 385 |
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series2 | Methods in molecular biology |
spelling | Post-translational modifications of proteins tools for functional proteomics Christoph Kannicht ed. 2. ed. Totowa, NJ Humana Press 2008 XVII, 390 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 446 This volume provides protocols of methods used to detect and analyse co- and post-translational modifications of proteins. It enables the reader to analyse the most important post-translational modifications focusing on proteome research. Post-translational modification Protein Processing, Post-Translational Laboratory Manuals Proteins analysis Laboratory Manuals Proteome analysis Laboratory Manuals Proteomics methods Laboratory Manuals Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Posttranslationale Änderung (DE-588)4204069-3 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Posttranslationale Änderung (DE-588)4204069-3 s Labortechnik (DE-588)4123602-6 s DE-604 Proteomanalyse (DE-588)4596545-6 s 1\p DE-604 Kannicht, Christoph Sonstige oth Methods in molecular biology 446 (DE-604)BV035362695 446 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016313029&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Post-translational modifications of proteins tools for functional proteomics Methods in molecular biology Post-translational modification Protein Processing, Post-Translational Laboratory Manuals Proteins analysis Laboratory Manuals Proteome analysis Laboratory Manuals Proteomics methods Laboratory Manuals Proteomanalyse (DE-588)4596545-6 gnd Labortechnik (DE-588)4123602-6 gnd Posttranslationale Änderung (DE-588)4204069-3 gnd Proteine (DE-588)4076388-2 gnd |
subject_GND | (DE-588)4596545-6 (DE-588)4123602-6 (DE-588)4204069-3 (DE-588)4076388-2 (DE-588)4143413-4 |
title | Post-translational modifications of proteins tools for functional proteomics |
title_auth | Post-translational modifications of proteins tools for functional proteomics |
title_exact_search | Post-translational modifications of proteins tools for functional proteomics |
title_exact_search_txtP | Post-translational modifications of proteins tools for functional proteomics |
title_full | Post-translational modifications of proteins tools for functional proteomics Christoph Kannicht ed. |
title_fullStr | Post-translational modifications of proteins tools for functional proteomics Christoph Kannicht ed. |
title_full_unstemmed | Post-translational modifications of proteins tools for functional proteomics Christoph Kannicht ed. |
title_short | Post-translational modifications of proteins |
title_sort | post translational modifications of proteins tools for functional proteomics |
title_sub | tools for functional proteomics |
topic | Post-translational modification Protein Processing, Post-Translational Laboratory Manuals Proteins analysis Laboratory Manuals Proteome analysis Laboratory Manuals Proteomics methods Laboratory Manuals Proteomanalyse (DE-588)4596545-6 gnd Labortechnik (DE-588)4123602-6 gnd Posttranslationale Änderung (DE-588)4204069-3 gnd Proteine (DE-588)4076388-2 gnd |
topic_facet | Post-translational modification Protein Processing, Post-Translational Laboratory Manuals Proteins analysis Laboratory Manuals Proteome analysis Laboratory Manuals Proteomics methods Laboratory Manuals Proteomanalyse Labortechnik Posttranslationale Änderung Proteine Aufsatzsammlung |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016313029&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT kannichtchristoph posttranslationalmodificationsofproteinstoolsforfunctionalproteomics |