RNai: design and application
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2008
|
| Series: | Methods in molecular biology
442 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIV, 270 S. Ill., graph. Darst. |
| ISBN: | 9781588298744 9781597451918 |
Staff View
MARC
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| 245 | 1 | 0 | |a RNai |b design and application |c ed. by Sailen Barik |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2008 | |
| 300 | |a XIV, 270 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 442 | |
| 650 | 4 | |a Gene Silencing |v Laboratory Manuals | |
| 650 | 4 | |a Genetic Techniques |v Laboratory Manuals | |
| 650 | 4 | |a RNA Interference |v Laboratory Manuals | |
| 650 | 4 | |a RNA, Small Interfering |v Laboratory Manuals | |
| 650 | 4 | |a Small interfering RNA |v Laboratory manuals | |
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface
...........................................................
vii
Contributors
...................................................... xi
Part I: Designing Optimal RNAi Tools
1.
Principles of Dicer Substrate (D-siRNA) Design and Function
Mohammed Amarzguioui and John J. Rossi
........................ 3
2.
Expression, Purification, and Analysis of
Recombinant
Drosophila Dicer-1
and Dicer-^ Enzymes
Xuecheng Ye and Qinghua Liu
.................................... 11
3.
In vitro
RNA
Cleavage Assay for Argonaute-Family Proteins
Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami-Okada,
Haruhiko Siomi, and Mikiko C.
Storni........................... 29
4.
Identifying siRNA-lnduced Off-Targets by Microarray Analysis
Emily Anderson,
Queta Boese, Anastasia
Khvorova,
and Jon Karpilow
............................................... 45
Part II: Application of RNAi in Diverse Organisms
5.
Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis
Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl,
Chun-Shiang Chung, and Alfred Ayala
.......................... 67
6.
Nasal Delivery of siRNA
Vira Bitko
and
Sauen
Barik
........................................ 75
7.
RNA
Interference as a Genetic Tool in Trypanosomes
Vivian Bellofatto and Jennifer B. Palenchar
........................ 83
8.
Lentivirus-Mediated
RNA
Interference in Mammalian Neurons
Scott Q. Harper and Pedro Gonzalez-Alegre
...................... 95
9.
Silencing Genes by
RNA
Interference in the Protozoan Parasite
Entamoeba histoh
tica
Carlos F.
Solis
and Nancy Guillen
................................. 113
ix
к
Contents
10.
Use of RNAi in
C. elegans
Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki,
and Fumio Hanaoka
............................................ 129
11.
Application of siRNA Against SARS in the Rhesus
Macaque Model
Qingquan Tang, Baojian Li, Martin Woodle, and Patrick
Y. Lu
.....139
12.
siRNA and shRNA as
Anticancer
Agents
in a Cervical Cancer Model
Weny
i Gu,
Lisa Putral, and Nigel McMillan
........................159
13.
Identification and Expression Analysis of Small RNAs During
Development
Toshiaki Watanabe, Hiroshi Imai, and Naojiro
Minami
.............173
14.
Screening and Identification of Virus-Encoded
RNA
Silencing
Suppressors
Sumona Karjee, Mohammad
Nurul
Islam,
and Sunil K. Mukherjee
.........................................187
15.
Application of
RNA
Interference in Functional Genomics Studies
of a Social Insect
Michael E.
Scharf,
Xuguo Zhou,
and Margaret A.
Schwinghammer..............................205
16.
Temporal Control of Gene Silencing by in
ovo Electroporation
Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli
...........231
1
7.
Altering Flower Color in Transgenic Plants by RNAi-Mediated
Engineering of Flavonoid Biosynthetic Pathway
Yoshikazu Tanaka, Noriko Nakamura, and Junichi
Togami
.........245
18.
Transgenic
RNA
Interference in Mice
Pumin Zhang
..................................................... 259
Index
............................................................. 267
|
| adam_txt |
Contents
Preface
.
vii
Contributors
. xi
Part I: Designing Optimal RNAi Tools
1.
Principles of Dicer Substrate (D-siRNA) Design and Function
Mohammed Amarzguioui and John J. Rossi
. 3
2.
Expression, Purification, and Analysis of
Recombinant
Drosophila Dicer-1
and Dicer-^ Enzymes
Xuecheng Ye and Qinghua Liu
. 11
3.
In vitro
RNA
Cleavage Assay for Argonaute-Family Proteins
Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami-Okada,
Haruhiko Siomi, and Mikiko C.
Storni. 29
4.
Identifying siRNA-lnduced Off-Targets by Microarray Analysis
Emily Anderson,
Queta Boese, Anastasia
Khvorova,
and Jon Karpilow
. 45
Part II: Application of RNAi in Diverse Organisms
5.
Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis
Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl,
Chun-Shiang Chung, and Alfred Ayala
. 67
6.
Nasal Delivery of siRNA
Vira Bitko
and
Sauen
Barik
. 75
7.
RNA
Interference as a Genetic Tool in Trypanosomes
Vivian Bellofatto and Jennifer B. Palenchar
. 83
8.
Lentivirus-Mediated
RNA
Interference in Mammalian Neurons
Scott Q. Harper and Pedro Gonzalez-Alegre
. 95
9.
Silencing Genes by
RNA
Interference in the Protozoan Parasite
Entamoeba histoh
tica
Carlos F.
Solis
and Nancy Guillen
. 113
ix
к
Contents
10.
Use of RNAi in
C. elegans
Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki,
and Fumio Hanaoka
. 129
11.
Application of siRNA Against SARS in the Rhesus
Macaque Model
Qingquan Tang, Baojian Li, Martin Woodle, and Patrick
Y. Lu
.139
12.
siRNA and shRNA as
Anticancer
Agents
in a Cervical Cancer Model
Weny
i Gu,
Lisa Putral, and Nigel McMillan
.159
13.
Identification and Expression Analysis of Small RNAs During
Development
Toshiaki Watanabe, Hiroshi Imai, and Naojiro
Minami
.173
14.
Screening and Identification of Virus-Encoded
RNA
Silencing
Suppressors
Sumona Karjee, Mohammad
Nurul
Islam,
and Sunil K. Mukherjee
.187
15.
Application of
RNA
Interference in Functional Genomics Studies
of a Social Insect
Michael E.
Scharf,
Xuguo Zhou,
and Margaret A.
Schwinghammer.205
16.
Temporal Control of Gene Silencing by in
ovo Electroporation
Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli
.231
1
7.
Altering Flower Color in Transgenic Plants by RNAi-Mediated
Engineering of Flavonoid Biosynthetic Pathway
Yoshikazu Tanaka, Noriko Nakamura, and Junichi
Togami
.245
18.
Transgenic
RNA
Interference in Mice
Pumin Zhang
. 259
Index
. 267 |
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| spelling | RNai design and application ed. by Sailen Barik Totowa, NJ Humana Press 2008 XIV, 270 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 442 Gene Silencing Laboratory Manuals Genetic Techniques Laboratory Manuals RNA Interference Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Labortechnik (DE-588)4123602-6 gnd rswk-swf RNS-Interferenz (DE-588)4802706-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS-Interferenz (DE-588)4802706-6 s Labortechnik (DE-588)4123602-6 s b DE-604 Barik, Sailen Sonstige oth Methods in molecular biology 442 (DE-604)BV035362695 442 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016312859&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RNai design and application Methods in molecular biology Gene Silencing Laboratory Manuals Genetic Techniques Laboratory Manuals RNA Interference Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Labortechnik (DE-588)4123602-6 gnd RNS-Interferenz (DE-588)4802706-6 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4802706-6 (DE-588)4143413-4 |
| title | RNai design and application |
| title_auth | RNai design and application |
| title_exact_search | RNai design and application |
| title_exact_search_txtP | RNai design and application |
| title_full | RNai design and application ed. by Sailen Barik |
| title_fullStr | RNai design and application ed. by Sailen Barik |
| title_full_unstemmed | RNai design and application ed. by Sailen Barik |
| title_short | RNai |
| title_sort | rnai design and application |
| title_sub | design and application |
| topic | Gene Silencing Laboratory Manuals Genetic Techniques Laboratory Manuals RNA Interference Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Labortechnik (DE-588)4123602-6 gnd RNS-Interferenz (DE-588)4802706-6 gnd |
| topic_facet | Gene Silencing Laboratory Manuals Genetic Techniques Laboratory Manuals RNA Interference Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Labortechnik RNS-Interferenz Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016312859&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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