Fluorescent proteins:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier AP
2008
|
| Edition: | 2. ed. |
| Series: | Methods in cell biology
85 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Auf der Rückseite des Titelblattes fälschlich als 1. ed. bezeichnet. - 1. Aufl. u.d.T.: Green fluorescent proteins |
| Physical Description: | XX, 592 S., [24] Bl. Ill., graph. Darst. |
| ISBN: | 9780123725585 |
Staff View
MARC
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| 020 | |a 9780123725585 |9 978-0-12-372558-5 | ||
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| 084 | |a WD 5100 |0 (DE-625)148194: |2 rvk | ||
| 245 | 1 | 0 | |a Fluorescent proteins |c ed. by Kevin F. Sullivan |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier AP |c 2008 | |
| 300 | |a XX, 592 S., [24] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 85 | |
| 500 | |a Auf der Rückseite des Titelblattes fälschlich als 1. ed. bezeichnet. - 1. Aufl. u.d.T.: Green fluorescent proteins | ||
| 650 | 4 | |a Biofluorescence | |
| 650 | 4 | |a Fluorescent polymers | |
| 650 | 4 | |a Proteins | |
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fluoreszenz |0 (DE-588)4154818-8 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Fluoreszenz |0 (DE-588)4154818-8 |D s |
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| 700 | 1 | |a Sullivan, Kevin Francis |d 1955- |e Sonstige |0 (DE-588)135581044 |4 oth | |
| 830 | 0 | |a Methods in cell biology |v 85 |w (DE-604)BV002534878 |9 85 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016275122&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Record in the Search Index
| _version_ | 1804137315533062144 |
|---|---|
| adam_text | CONTENTS
Contributors
Preface
1.
Autofltiorescent Proteins
Лі»
Л/.
Dobbic, Soel
1·.
Lourdes,
and Kevin I· .
Sullivan
1.
History
2
II. Variants
5
III. Practical Considerations
11
IV. Advanced FP Applications
13
V. Future Directions
17
References
1
H
2.
Functional Fusion Proteins by Random Transposon-Based GFP Insertion
Robert Mealer, Heather Butler, and
Tilomas
Hughes
I. Introduction
24
II. Rationale
27
III. Methods
31
IV. Materials
40
V. Discussion
42
References
43
3.
Fluorescent Proteins tor Photoactivation Experiments
Jennifer
Lippiiiíútt-Schtrart:
and George H. Patterson
I. Why Use a Fluorescent Protein?
46
II. Why Use a Photoactivatable Fluorescent Protein?
46
III. Survey of Photoactivatable Fluorescent Proteins
47
IV. Uses of Photoactivatable Fluorescent Proteins
52
V. Future Directions ot Photoactivatable Fluorescent Proteins
58
References
59
Contents
4. Design
and Optimization of Genetically Encoded Fluorescent Biosensors:
GTPase Biosensors
Louts Hodgson, Olivier Pertz, and Klaus M. Halm
I. Introduction
64
II. Background: Factors Influencing FRET Efficiency
66
III. Design and Cloning of Biosensors
67
IV. Validation of the Biosensor in Cell Suspensions
69
V. Microscopy and Imaging Considerations
73
VI. Conclusion
75
VII.
Appendix I
77
VIII.
Appendix II
79
References
79
5.
Fast 4D Microscopy
J. R.
De
Mey, P.
Kessler,]. Dompierre,
F. P. Cordelières, A.
Dietenen,
J.-L.
Vonesch, andJ.-B.
Sibarita
I. Introduction
84
Ii.
Fast 4D Imaging: Definition, Interest, and Limits
87
III. Points to Consider Before Working with Fast 4D Imaging Systems
89
IV. Conclusions
107
References
110
6.
Single-Molecule Imaging ot Fluorescent Proteins
Adam D. Douglass and Rotiald D. I ale
I. Introduction
114
II. Instrumentation
115
III. Fluorophores
118
IV. Reducing Protein Expression Levels
119
V. Biological Preparations
121
VI. Data Analysis and Interpretation
122
VII.
Future Prospects
123
References
124
7.
Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically
Encoded Fluorescent Proteins
Ajit P. Joglekar. E. D.
Salinou,
and Kerry S. Bloom
I. Introduction
128
II. Counting Kinetochore Protein Numbers in Budding Yeast
130
III. Sample Preparation
134
Contents
IV.
Microscope
and Image Acquisition System
135
V. Measurement of Flviorescence Signal
137
VI. Validation of Measurement Method
140
VII.
Results
142
VIII.
Discussion
144
IX. Conclusions
148
References
149
Fluorescent Protein Applications in Plants
R. Howard Berg and Roger X. Beacliy
I. Introduction
154
II. Expression and Function of FPs in Plants
155
III. Imaging
164
IV. Advanced Techniques
170
V. Summan
173
References
174
9.
Expression and Imaging of Fluorescent Proteins in the
C elegans
Gonad
and Early Embryo
Rebecca A. Green,
Anjou
Audhya, Andrei Po:niakovsky, Alexander
Dammermann,
Науку
Penible,
Joost
Moneti,
Xathan
Portier, Anthony Hynian, Arshad Desai,
and Karen
Ocgema
I. Introduction
180
II. Fluorescent Proteins in the
C. elegans
Gonad
and Early Embryo
183
III.
Transgene
Expression in the
C. elegans
Germ Line: Breaking the Silence
187
ÌV.
Constructing Fluorescent Worm Lines
188
V. Using Fluorescent Worm Strains
202
VI.
Summan-
210
Appendix
211
References
213
10.
Fluorescent Proteins in Zebrafish Cell and Developmental Biology
H. William Derrick, III
I. Introduction
220
II. Zebrafish Kinesin Genes in Early Development: A Cytokinetic
Role for zMklp
1 221
III. Cell-Specific. Laser-Induced
Transgene
Expression in the
Zebrafish Embryo: The
ЅешаЗа
I Gene in Axonal Guidance
226
IV.
Transgeni
c Zebrafish
Models of Myc-Induced T-Cell Acute
Lymphoblastic Leukemia
232
V. Summan
236
References
237
Contents
11.
Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain
Juan Manuel Encinas
and Grigori Enikolopov
I. Introduction
244
II. Protocol I: Immimofluorescence Microscopy of Nestin-GFP and
Nestin-CFPnuc Cells
254
III. Protocol II: The Use of Confocal
Stereologţ
to Quantify Changes
in Defined Classes of
Neuronal
Precursors
258
IV. Protocol III·. Electron Microscopy of Nestin-GFP/CFPnuc Cells
265
References
270
12.
Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells
Emmanuelle
Querido
and Pascal
Chartrand
I. Introduction
274
II. The MS2-GFP System
274
III. RNA
Trafficking in
Fibroblasts
278
IV. Following
RNA
Trafficking in Living Yeasts
287
References
291
13.
Visualizing mRNA Localization and Local Protein Translation in Neurons
Ralf Dahin,
Manuel Zcitelhofer,
Bernhard Götze,
Michael A. Kiebler, and Paolo
Macchi
I. Introduction
294
II. Visualization of
RNA
Transport via RNA-Binding Proteins in Neurons
296
III. Visualization of RNP Transport
308
IV. Visualization of RNP Assembly and Composition
31
fi
V. Visualization of Interactions Between RNAs and fra/w-Acting Factors
318
VI. Visualization of Local mRNA Translation
320
VII.
Outlook
323
References
324
14.
Quantitative FRAP in Analysis of Molecular Binding Dynamics in l
ivo
James G. McXally
1.
Introduction
330
II. Rationale
330
HI. Methods
331
IV. Materials
348
V. Discussion
348
VI.
Summan·
349
References
350
Contents
15. Quantitative and Qualitative
Analysis of
Plant Membrane
Traffic Using
Fluorescent
Proteins
Marketa
Samalova,
Mark Flicker, and ¡an Moon·
I. Introduction
354
II. Rationale
362
III. Material
365
IV. Methods
365
V. Discussion
375
VI. Summary
378
References
378
16.
Engineering FRET Constructs Using CFP and YFP
Satosld Slmnozoiio and Atsushi Mtyaivaki
I. Introduction
382
II. Rationale
382
III. Methods
384
References
393
17.
Fluorescence Anisotropy Imaging Microscopy for Homo-FRET in Living Cells
Marc Trainier
and
Malte
Coppcy-Moisan
I. Introduction
396
II. Photoselection Process
396
III. Rotational Depolarization
398
IV. Experimental Measurement of Fluorescence Anisotropy Decay
in Confocal Microscopy
399
V. Fluorescence Anisotropy Decay of GFP-Tagged Proteins
402
VI. Fluorescence Depolarization by Homo-FRET
403
VII.
Steady-State Fluorescence Anisotropy Imaging
406
VIII.
Imaging Homo-FRET by Two-Photon
FAIM
409
IX. Biological Applications with GFP-Tagged Proteins
410
X. Conclusion
413
References
413
18.
FRET by Fluorescence Polarization Microscopy
David
И
Piste» and Mark
A. Rizzo
I. Introduction
416
II. Measuring FRET by Polarization Microscopy
418
III. Configuration of Microscopes for AFRET
419
IV. Calculation of Fluorescence Anisotropy
424
Contents
V.
Sample
Preparation
426
VI. Conclusions
429
References
429
19.
Bimolecular Fluorescence Complementation: Visualization of Molecular
Interactions in Living Cells
Tom K. Kirppola
I. Introduction
433
II. Approaches for the Investigation of Protein Interactions
434
III. Bimolecular Fluorescence Complementation Analysis
439
IV. Experimental Strategies for BiFC Analysis
444
V. Examples of Protein Interactions That Have Been Visualized
Using the BiFC Assay
449
VI. BiFC Analysis of Interactions in Different Organisms
454
VII.
Screens Using the BiFC Approach
455
VIII.
Analysis of Complex Dynamics Using the BiFC Approach
455
IX. Simultaneous Visualization of Several Protein Complexes
456
X. Experimental Strategies for Multicolor BiFC Analysis
458
XI. Limitations of the Multicolor BiFC Assay for Analysis of the
Efficiencies of Protein Interactions in Cells
460
XII.
Interaction Partners Whose Competition Has Been Visualized
Using the Multicolor BiFC Assay
460
XIII.
Visualization of Ubiquitin Family
Peptide
Conjugates in Cells
461
XIV.
Ubiquitin Family
Peptide
Conjugates That Have Been Visualized
Using the UbFC Assay
462
XV. Comparison of BiFC Analysis with Other Methods for the
Visualization of Protein Interactions in Living Cells
463
XVI.
Future Opportunities and Challenges
464
References
465
20.
Protein—Protein Interactions Determined by Fluorescence Correlation Spectroscopy
I. Introduction
472
II. FCS Theory
475
III. Two-Color Cross-Correlation
477
IV. Protein—Protein Interactions Using FCCS and Nongenetic Labels 47H
V. Protein—Protein Interactions In Vivo Using FCCS and
Autofluorescent Proteins
479
References
482
Contents
21.
Recent
Advances on In Vivo Imaging with Fluorescent Proteins
Robert M. Hoffman
I. Macroimaging with Fluorescent Proteins
486
II. Single-Cell In Vivo Imaging with Fluorescent Proteins
487
III. Imaging Dual-Color Angiogenesis and Tumors with Fluorescent Properties
488
IV. Imaging Tumor-Host Interaction with Fluorescent Proteins
489
V. New Applications for Fluorescent Proteins In Vivo: The Development
of Effective Bacterial Therapy of Cancer
491
VI. Conclusions
492
References
493
22.
Computational Processing and Analysis of Dynamic Fluorescence Image Data
Jonas
F. Dorn,
Ganden:
Dannser, and Ge Yang
I. Introduction
498
II. Rationale
499
III. Image Features and Representation of Dynamic Events
502
IV. Methods
505
V. Two Case Studies of Image Analysis Applied to Mechanistic Cell Biology
520
VI. Performance Evaluation for Quality Control
527
VII.
Summary
532
References
533
23.
Automated Classification of Mitotic Phenotypes of Human Cells Using
Fluorescent Proteins
Л*.
Harder, R. Eils, and K.
Rohr
I. Introduction
540
II. Segmentation of
Multiceli
Images
541
III. Extraction of Image Features
544
IV. Image Features
545
V. Classification ot Mitotic Patterns
547
VI. Experimental Results
548
VIII.
Conclusion
552
References
553
24.
Open Tools for Storage and Management ot Quantitative Image Data
Joshua Moore, Chris Allan, Jean-Marie
Burel,
Brian Granger, Dotiald MacDonald,
Jonathan Monk, and Jason R. Swedlow
I. Introduction
556
II. Secure, Archived and Available Storage for Biological Image Data
557
Contents
III. The Open Microscopy Environment: Data Management Tools
for Biological Research
559
IV. The
OMERO
Server: A New Server Application for Data Management
561
V. Future Directions
569
References
570
Index
571
Volumes in Series
585
|
| adam_txt |
CONTENTS
Contributors
Preface
1.
Autofltiorescent Proteins
Лі»
Л/.
Dobbic, Soel
1·.
Lourdes,
and Kevin I·'.
Sullivan
1.
History
2
II. Variants
5
III. Practical Considerations
11
IV. Advanced FP Applications
13
V. Future Directions
17
References
1
H
2.
Functional Fusion Proteins by Random Transposon-Based GFP Insertion
Robert Mealer, Heather Butler, and
Tilomas
Hughes
I. Introduction
24
II. Rationale
27
III. Methods
31
IV. Materials
40
V. Discussion
42
References
43
3.
Fluorescent Proteins tor Photoactivation Experiments
Jennifer
Lippiiiíútt-Schtrart:
and George H. Patterson
I. Why Use a Fluorescent Protein?
46
II. Why Use a Photoactivatable Fluorescent Protein?
46
III. Survey of Photoactivatable Fluorescent Proteins
47
IV. Uses of Photoactivatable Fluorescent Proteins
52
V. Future Directions ot Photoactivatable Fluorescent Proteins
58
References
59
Contents
4. Design
and Optimization of Genetically Encoded Fluorescent Biosensors:
GTPase Biosensors
Louts Hodgson, Olivier Pertz, and Klaus M. Halm
I. Introduction
64
II. Background: Factors Influencing FRET Efficiency
66
III. Design and Cloning of Biosensors
67
IV. Validation of the Biosensor in Cell Suspensions
69
V. Microscopy and Imaging Considerations
73
VI. Conclusion
75
VII.
Appendix I
77
VIII.
Appendix II
79
References
79
5.
Fast 4D Microscopy
J. R.
De
Mey, P.
Kessler,]. Dompierre,
F. P. Cordelières, A.
Dietenen,
J.-L.
Vonesch, andJ.-B.
Sibarita
I. Introduction
84
Ii.
Fast 4D Imaging: Definition, Interest, and Limits
87
III. Points to Consider Before Working with Fast 4D Imaging Systems
89
IV. Conclusions
107
References
110
6.
Single-Molecule Imaging ot Fluorescent Proteins
Adam D. Douglass and Rotiald D. I 'ale
I. Introduction
114
II. Instrumentation
115
III. Fluorophores
118
IV. Reducing Protein Expression Levels
119
V. Biological Preparations
121
VI. Data Analysis and Interpretation
122
VII.
Future Prospects
123
References
124
7.
Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically
Encoded Fluorescent Proteins
Ajit P. Joglekar. E. D.
Salinou,
and Kerry S. Bloom
I. Introduction
128
II. Counting Kinetochore Protein Numbers in Budding Yeast
130
III. Sample Preparation
134
Contents
IV.
Microscope
and Image Acquisition System
135
V. Measurement of Flviorescence Signal
137
VI. Validation of Measurement Method
140
VII.
Results
142
VIII.
Discussion
144
IX. Conclusions
148
References
149
Fluorescent Protein Applications in Plants
R. Howard Berg and Roger X. Beacliy
I. Introduction
154
II. Expression and Function of FPs in Plants
155
III. Imaging
164
IV. Advanced Techniques
170
V. Summan'
173
References
174
9.
Expression and Imaging of Fluorescent Proteins in the
C elegans
Gonad
and Early Embryo
Rebecca A. Green,
Anjou
Audhya, Andrei Po:niakovsky, Alexander
Dammermann,
Науку
Penible,
Joost
Moneti,
Xathan
Portier, Anthony Hynian, Arshad Desai,
and Karen
Ocgema
I. Introduction
180
II. Fluorescent Proteins in the
C. elegans
Gonad
and Early Embryo
183
III.
Transgene
Expression in the
C. elegans
Germ Line: Breaking the Silence
187
ÌV.
Constructing Fluorescent Worm Lines
188
V. Using Fluorescent Worm Strains
202
VI.
Summan-
210
Appendix
211
References
213
10.
Fluorescent Proteins in Zebrafish Cell and Developmental Biology
H. William Derrick, III
I. Introduction
220
II. Zebrafish Kinesin Genes in Early Development: A Cytokinetic
Role for zMklp
1 221
III. Cell-Specific. Laser-Induced
Transgene
Expression in the
Zebrafish Embryo: The
ЅешаЗа
I Gene in Axonal Guidance
226
IV.
Transgeni
c Zebrafish
Models of Myc-Induced T-Cell Acute
Lymphoblastic Leukemia
232
V. Summan'
236
References
237
Contents
11.
Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain
Juan Manuel Encinas
and Grigori Enikolopov
I. Introduction
244
II. Protocol I: Immimofluorescence Microscopy of Nestin-GFP and
Nestin-CFPnuc Cells
254
III. Protocol II: The Use of Confocal
Stereologţ'
to Quantify Changes
in Defined Classes of
Neuronal
Precursors
258
IV. Protocol III·. Electron Microscopy of Nestin-GFP/CFPnuc Cells
265
References
270
12.
Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells
Emmanuelle
Querido
and Pascal
Chartrand
I. Introduction
274
II. The MS2-GFP System
274
III. RNA
Trafficking in
Fibroblasts
278
IV. Following
RNA
Trafficking in Living Yeasts
287
References
291
13.
Visualizing mRNA Localization and Local Protein Translation in Neurons
Ralf Dahin,
Manuel Zcitelhofer,
Bernhard Götze,
Michael A. Kiebler, and Paolo
Macchi
I. Introduction
294
II. Visualization of
RNA
Transport via RNA-Binding Proteins in Neurons
296
III. Visualization of RNP Transport
308
IV. Visualization of RNP Assembly and Composition
31
fi
V. Visualization of Interactions Between RNAs and fra/w-Acting Factors
318
VI. Visualization of Local mRNA Translation
320
VII.
Outlook
323
References
324
14.
Quantitative FRAP in Analysis of Molecular Binding Dynamics in l
'ivo
James G. McXally
1.
Introduction
330
II. Rationale
330
HI. Methods
331
IV. Materials
348
V. Discussion
348
VI.
Summan·
349
References
350
Contents
15. Quantitative and Qualitative
Analysis of
Plant Membrane
Traffic Using
Fluorescent
Proteins
Marketa
Samalova,
Mark Flicker, and ¡an Moon·
I. Introduction
354
II. Rationale
362
III. Material
365
IV. Methods
365
V. Discussion
375
VI. Summary
378
References
378
16.
Engineering FRET Constructs Using CFP and YFP
Satosld Slmnozoiio and Atsushi Mtyaivaki
I. Introduction
382
II. Rationale
382
III. Methods
384
References
393
17.
Fluorescence Anisotropy Imaging Microscopy for Homo-FRET in Living Cells
Marc Trainier
and
Malte
Coppcy-Moisan
I. Introduction
396
II. Photoselection Process
396
III. Rotational Depolarization
398
IV. Experimental Measurement of Fluorescence Anisotropy Decay
in Confocal Microscopy
399
V. Fluorescence Anisotropy Decay of GFP-Tagged Proteins
402
VI. Fluorescence Depolarization by Homo-FRET
403
VII.
Steady-State Fluorescence Anisotropy Imaging
406
VIII.
Imaging Homo-FRET by Two-Photon
FAIM
409
IX. Biological Applications with GFP-Tagged Proteins
410
X. Conclusion
413
References
413
18.
FRET by Fluorescence Polarization Microscopy
David
И"
Piste» and Mark
A. Rizzo
I. Introduction
416
II. Measuring FRET by Polarization Microscopy
418
III. Configuration of Microscopes for AFRET
419
IV. Calculation of Fluorescence Anisotropy
424
Contents
V.
Sample
Preparation
426
VI. Conclusions
429
References
429
19.
Bimolecular Fluorescence Complementation: Visualization of Molecular
Interactions in Living Cells
Tom K. Kirppola
I. Introduction
433
II. Approaches for the Investigation of Protein Interactions
434
III. Bimolecular Fluorescence Complementation Analysis
439
IV. Experimental Strategies for BiFC Analysis
444
V. Examples of Protein Interactions That Have Been Visualized
Using the BiFC Assay
449
VI. BiFC Analysis of Interactions in Different Organisms
454
VII.
Screens Using the BiFC Approach
455
VIII.
Analysis of Complex Dynamics Using the BiFC Approach
455
IX. Simultaneous Visualization of Several Protein Complexes
456
X. Experimental Strategies for Multicolor BiFC Analysis
458
XI. Limitations of the Multicolor BiFC Assay for Analysis of the
Efficiencies of Protein Interactions in Cells
460
XII.
Interaction Partners Whose Competition Has Been Visualized
Using the Multicolor BiFC Assay
460
XIII.
Visualization of Ubiquitin Family
Peptide
Conjugates in Cells
461
XIV.
Ubiquitin Family
Peptide
Conjugates That Have Been Visualized
Using the UbFC Assay
462
XV. Comparison of BiFC Analysis with Other Methods for the
Visualization of Protein Interactions in Living Cells
463
XVI.
Future Opportunities and Challenges
464
References
465
20.
Protein—Protein Interactions Determined by Fluorescence Correlation Spectroscopy
I. Introduction
472
II. FCS Theory
475
III. Two-Color Cross-Correlation
477
IV. Protein—Protein Interactions Using FCCS and Nongenetic Labels 47H
V. Protein—Protein Interactions In Vivo Using FCCS and
Autofluorescent Proteins
479
References
482
Contents
21.
Recent
Advances on In Vivo Imaging with Fluorescent Proteins
Robert M. Hoffman
I. Macroimaging with Fluorescent Proteins
486
II. Single-Cell In Vivo Imaging with Fluorescent Proteins
487
III. Imaging Dual-Color Angiogenesis and Tumors with Fluorescent Properties
488
IV. Imaging Tumor-Host Interaction with Fluorescent Proteins
489
V. New Applications for Fluorescent Proteins In Vivo: The Development
of Effective Bacterial Therapy of Cancer
491
VI. Conclusions
492
References
493
22.
Computational Processing and Analysis of Dynamic Fluorescence Image Data
Jonas
F. Dorn,
Ganden:
Dannser, and Ge Yang
I. Introduction
498
II. Rationale
499
III. Image Features and Representation of Dynamic Events
502
IV. Methods
505
V. Two Case Studies of Image Analysis Applied to Mechanistic Cell Biology
520
VI. Performance Evaluation for Quality Control
527
VII.
Summary
532
References
533
23.
Automated Classification of Mitotic Phenotypes of Human Cells Using
Fluorescent Proteins
Л*.
Harder, R. Eils, and K.
Rohr
I. Introduction
540
II. Segmentation of
Multiceli
Images
541
III. Extraction of Image Features
544
IV. Image Features
545
V. Classification ot Mitotic Patterns
547
VI. Experimental Results
548
VIII.
Conclusion
552
References
553
24.
Open Tools for Storage and Management ot Quantitative Image Data
Joshua Moore, Chris Allan, Jean-Marie
Burel,
Brian Granger, Dotiald MacDonald,
Jonathan Monk, and Jason R. Swedlow
I. Introduction
556
II. Secure, Archived and Available Storage for Biological Image Data
557
Contents
III. The Open Microscopy Environment: Data Management Tools
for Biological Research
559
IV. The
OMERO
Server: A New Server Application for Data Management
561
V. Future Directions
569
References
570
Index
571
Volumes in Series
585 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author_GND | (DE-588)135581044 |
| building | Verbundindex |
| bvnumber | BV023071984 |
| callnumber-first | Q - Science |
| callnumber-label | QH585 |
| callnumber-raw | QH585 |
| callnumber-search | QH585 |
| callnumber-sort | QH 3585 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 5100 WD 5100 |
| ctrlnum | (OCoLC)190966847 (DE-599)BVBBV023071984 |
| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| edition | 2. ed. |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV023071984 |
| illustrated | Illustrated |
| index_date | 2024-07-02T19:33:23Z |
| indexdate | 2024-07-09T21:10:21Z |
| institution | BVB |
| isbn | 9780123725585 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016275122 |
| oclc_num | 190966847 |
| open_access_boolean | |
| owner | DE-703 DE-355 DE-BY-UBR DE-20 |
| owner_facet | DE-703 DE-355 DE-BY-UBR DE-20 |
| physical | XX, 592 S., [24] Bl. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Elsevier AP |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Fluorescent proteins ed. by Kevin F. Sullivan 2. ed. Amsterdam [u.a.] Elsevier AP 2008 XX, 592 S., [24] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 85 Auf der Rückseite des Titelblattes fälschlich als 1. ed. bezeichnet. - 1. Aufl. u.d.T.: Green fluorescent proteins Biofluorescence Fluorescent polymers Proteins Proteine (DE-588)4076388-2 gnd rswk-swf Fluoreszenz (DE-588)4154818-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Fluoreszenz (DE-588)4154818-8 s b DE-604 Sullivan, Kevin Francis 1955- Sonstige (DE-588)135581044 oth Methods in cell biology 85 (DE-604)BV002534878 85 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016275122&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fluorescent proteins Methods in cell biology Biofluorescence Fluorescent polymers Proteins Proteine (DE-588)4076388-2 gnd Fluoreszenz (DE-588)4154818-8 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4154818-8 (DE-588)4143413-4 |
| title | Fluorescent proteins |
| title_auth | Fluorescent proteins |
| title_exact_search | Fluorescent proteins |
| title_exact_search_txtP | Fluorescent proteins |
| title_full | Fluorescent proteins ed. by Kevin F. Sullivan |
| title_fullStr | Fluorescent proteins ed. by Kevin F. Sullivan |
| title_full_unstemmed | Fluorescent proteins ed. by Kevin F. Sullivan |
| title_short | Fluorescent proteins |
| title_sort | fluorescent proteins |
| topic | Biofluorescence Fluorescent polymers Proteins Proteine (DE-588)4076388-2 gnd Fluoreszenz (DE-588)4154818-8 gnd |
| topic_facet | Biofluorescence Fluorescent polymers Proteins Proteine Fluoreszenz Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016275122&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT sullivankevinfrancis fluorescentproteins |