Verfügbarkeit: Fluorescent proteins

Fluorescent proteins:

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Format:	Buch
Sprache:	English
Veröffentlicht:	Amsterdam [u.a.] Elsevier AP 2008
Ausgabe:	2. ed.
Schriftenreihe:	Methods in cell biology 85
Schlagworte:	Biofluorescence Fluorescent polymers Proteins Proteine Fluoreszenz Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	Auf der Rückseite des Titelblattes fälschlich als 1. ed. bezeichnet. - 1. Aufl. u.d.T.: Green fluorescent proteins
Beschreibung:	XX, 592 S., [24] Bl. Ill., graph. Darst.
ISBN:	9780123725585

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Datensatz im Suchindex

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adam_text	CONTENTS Contributors Preface 1. Autofltiorescent Proteins Лі» Л/. Dobbic, Soel 1·. Lourdes, and Kevin I· . Sullivan 1. History 2 II. Variants 5 III. Practical Considerations 11 IV. Advanced FP Applications 13 V. Future Directions 17 References 1 H 2. Functional Fusion Proteins by Random Transposon-Based GFP Insertion Robert Mealer, Heather Butler, and Tilomas Hughes I. Introduction 24 II. Rationale 27 III. Methods 31 IV. Materials 40 V. Discussion 42 References 43 3. Fluorescent Proteins tor Photoactivation Experiments Jennifer Lippiiiíútt-Schtrart: and George H. Patterson I. Why Use a Fluorescent Protein? 46 II. Why Use a Photoactivatable Fluorescent Protein? 46 III. Survey of Photoactivatable Fluorescent Proteins 47 IV. Uses of Photoactivatable Fluorescent Proteins 52 V. Future Directions ot Photoactivatable Fluorescent Proteins 58 References 59 Contents 4. Design and Optimization of Genetically Encoded Fluorescent Biosensors: GTPase Biosensors Louts Hodgson, Olivier Pertz, and Klaus M. Halm I. Introduction 64 II. Background: Factors Influencing FRET Efficiency 66 III. Design and Cloning of Biosensors 67 IV. Validation of the Biosensor in Cell Suspensions 69 V. Microscopy and Imaging Considerations 73 VI. Conclusion 75 VII. Appendix I 77 VIII. Appendix II 79 References 79 5. Fast 4D Microscopy J. R. De Mey, P. Kessler,]. Dompierre, F. P. Cordelières, A. Dietenen, J.-L. Vonesch, andJ.-B. Sibarita I. Introduction 84 Ii. Fast 4D Imaging: Definition, Interest, and Limits 87 III. Points to Consider Before Working with Fast 4D Imaging Systems 89 IV. Conclusions 107 References 110 6. Single-Molecule Imaging ot Fluorescent Proteins Adam D. Douglass and Rotiald D. I ale I. Introduction 114 II. Instrumentation 115 III. Fluorophores 118 IV. Reducing Protein Expression Levels 119 V. Biological Preparations 121 VI. Data Analysis and Interpretation 122 VII. Future Prospects 123 References 124 7. Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins Ajit P. Joglekar. E. D. Salinou, and Kerry S. Bloom I. Introduction 128 II. Counting Kinetochore Protein Numbers in Budding Yeast 130 III. Sample Preparation 134 Contents IV. Microscope and Image Acquisition System 135 V. Measurement of Flviorescence Signal 137 VI. Validation of Measurement Method 140 VII. Results 142 VIII. Discussion 144 IX. Conclusions 148 References 149 Fluorescent Protein Applications in Plants R. Howard Berg and Roger X. Beacliy I. Introduction 154 II. Expression and Function of FPs in Plants 155 III. Imaging 164 IV. Advanced Techniques 170 V. Summan 173 References 174 9. Expression and Imaging of Fluorescent Proteins in the C elegans Gonad and Early Embryo Rebecca A. Green, Anjou Audhya, Andrei Po:niakovsky, Alexander Dammermann, Науку Penible, Joost Moneti, Xathan Portier, Anthony Hynian, Arshad Desai, and Karen Ocgema I. Introduction 180 II. Fluorescent Proteins in the C. elegans Gonad and Early Embryo 183 III. Transgene Expression in the C. elegans Germ Line: Breaking the Silence 187 ÌV. Constructing Fluorescent Worm Lines 188 V. Using Fluorescent Worm Strains 202 VI. Summan- 210 Appendix 211 References 213 10. Fluorescent Proteins in Zebrafish Cell and Developmental Biology H. William Derrick, III I. Introduction 220 II. Zebrafish Kinesin Genes in Early Development: A Cytokinetic Role for zMklp 1 221 III. Cell-Specific. Laser-Induced Transgene Expression in the Zebrafish Embryo: The ЅешаЗа I Gene in Axonal Guidance 226 IV. Transgeni c Zebrafish Models of Myc-Induced T-Cell Acute Lymphoblastic Leukemia 232 V. Summan 236 References 237 Contents 11. Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain Juan Manuel Encinas and Grigori Enikolopov I. Introduction 244 II. Protocol I: Immimofluorescence Microscopy of Nestin-GFP and Nestin-CFPnuc Cells 254 III. Protocol II: The Use of Confocal Stereologţ to Quantify Changes in Defined Classes of Neuronal Precursors 258 IV. Protocol III·. Electron Microscopy of Nestin-GFP/CFPnuc Cells 265 References 270 12. Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells Emmanuelle Querido and Pascal Chartrand I. Introduction 274 II. The MS2-GFP System 274 III. RNA Trafficking in Fibroblasts 278 IV. Following RNA Trafficking in Living Yeasts 287 References 291 13. Visualizing mRNA Localization and Local Protein Translation in Neurons Ralf Dahin, Manuel Zcitelhofer, Bernhard Götze, Michael A. Kiebler, and Paolo Macchi I. Introduction 294 II. Visualization of RNA Transport via RNA-Binding Proteins in Neurons 296 III. Visualization of RNP Transport 308 IV. Visualization of RNP Assembly and Composition 31 fi V. Visualization of Interactions Between RNAs and fra/w-Acting Factors 318 VI. Visualization of Local mRNA Translation 320 VII. Outlook 323 References 324 14. Quantitative FRAP in Analysis of Molecular Binding Dynamics in l ivo James G. McXally 1. Introduction 330 II. Rationale 330 HI. Methods 331 IV. Materials 348 V. Discussion 348 VI. Summan· 349 References 350 Contents 15. Quantitative and Qualitative Analysis of Plant Membrane Traffic Using Fluorescent Proteins Marketa Samalova, Mark Flicker, and ¡an Moon· I. Introduction 354 II. Rationale 362 III. Material 365 IV. Methods 365 V. Discussion 375 VI. Summary 378 References 378 16. Engineering FRET Constructs Using CFP and YFP Satosld Slmnozoiio and Atsushi Mtyaivaki I. Introduction 382 II. Rationale 382 III. Methods 384 References 393 17. Fluorescence Anisotropy Imaging Microscopy for Homo-FRET in Living Cells Marc Trainier and Malte Coppcy-Moisan I. Introduction 396 II. Photoselection Process 396 III. Rotational Depolarization 398 IV. Experimental Measurement of Fluorescence Anisotropy Decay in Confocal Microscopy 399 V. Fluorescence Anisotropy Decay of GFP-Tagged Proteins 402 VI. Fluorescence Depolarization by Homo-FRET 403 VII. Steady-State Fluorescence Anisotropy Imaging 406 VIII. Imaging Homo-FRET by Two-Photon FAIM 409 IX. Biological Applications with GFP-Tagged Proteins 410 X. Conclusion 413 References 413 18. FRET by Fluorescence Polarization Microscopy David И Piste» and Mark A. Rizzo I. Introduction 416 II. Measuring FRET by Polarization Microscopy 418 III. Configuration of Microscopes for AFRET 419 IV. Calculation of Fluorescence Anisotropy 424 Contents V. Sample Preparation 426 VI. Conclusions 429 References 429 19. Bimolecular Fluorescence Complementation: Visualization of Molecular Interactions in Living Cells Tom K. Kirppola I. Introduction 433 II. Approaches for the Investigation of Protein Interactions 434 III. Bimolecular Fluorescence Complementation Analysis 439 IV. Experimental Strategies for BiFC Analysis 444 V. Examples of Protein Interactions That Have Been Visualized Using the BiFC Assay 449 VI. BiFC Analysis of Interactions in Different Organisms 454 VII. Screens Using the BiFC Approach 455 VIII. Analysis of Complex Dynamics Using the BiFC Approach 455 IX. Simultaneous Visualization of Several Protein Complexes 456 X. Experimental Strategies for Multicolor BiFC Analysis 458 XI. Limitations of the Multicolor BiFC Assay for Analysis of the Efficiencies of Protein Interactions in Cells 460 XII. Interaction Partners Whose Competition Has Been Visualized Using the Multicolor BiFC Assay 460 XIII. Visualization of Ubiquitin Family Peptide Conjugates in Cells 461 XIV. Ubiquitin Family Peptide Conjugates That Have Been Visualized Using the UbFC Assay 462 XV. Comparison of BiFC Analysis with Other Methods for the Visualization of Protein Interactions in Living Cells 463 XVI. Future Opportunities and Challenges 464 References 465 20. Protein—Protein Interactions Determined by Fluorescence Correlation Spectroscopy I. Introduction 472 II. FCS Theory 475 III. Two-Color Cross-Correlation 477 IV. Protein—Protein Interactions Using FCCS and Nongenetic Labels 47H V. Protein—Protein Interactions In Vivo Using FCCS and Autofluorescent Proteins 479 References 482 Contents 21. Recent Advances on In Vivo Imaging with Fluorescent Proteins Robert M. Hoffman I. Macroimaging with Fluorescent Proteins 486 II. Single-Cell In Vivo Imaging with Fluorescent Proteins 487 III. Imaging Dual-Color Angiogenesis and Tumors with Fluorescent Properties 488 IV. Imaging Tumor-Host Interaction with Fluorescent Proteins 489 V. New Applications for Fluorescent Proteins In Vivo: The Development of Effective Bacterial Therapy of Cancer 491 VI. Conclusions 492 References 493 22. Computational Processing and Analysis of Dynamic Fluorescence Image Data Jonas F. Dorn, Ganden: Dannser, and Ge Yang I. Introduction 498 II. Rationale 499 III. Image Features and Representation of Dynamic Events 502 IV. Methods 505 V. Two Case Studies of Image Analysis Applied to Mechanistic Cell Biology 520 VI. Performance Evaluation for Quality Control 527 VII. Summary 532 References 533 23. Automated Classification of Mitotic Phenotypes of Human Cells Using Fluorescent Proteins Л*. Harder, R. Eils, and K. Rohr I. Introduction 540 II. Segmentation of Multiceli Images 541 III. Extraction of Image Features 544 IV. Image Features 545 V. Classification ot Mitotic Patterns 547 VI. Experimental Results 548 VIII. Conclusion 552 References 553 24. Open Tools for Storage and Management ot Quantitative Image Data Joshua Moore, Chris Allan, Jean-Marie Burel, Brian Granger, Dotiald MacDonald, Jonathan Monk, and Jason R. Swedlow I. Introduction 556 II. Secure, Archived and Available Storage for Biological Image Data 557 Contents III. The Open Microscopy Environment: Data Management Tools for Biological Research 559 IV. The OMERO Server: A New Server Application for Data Management 561 V. Future Directions 569 References 570 Index 571 Volumes in Series 585
adam_txt	CONTENTS Contributors Preface 1. Autofltiorescent Proteins Лі» Л/. Dobbic, Soel 1·. Lourdes, and Kevin I·'. Sullivan 1. History 2 II. Variants 5 III. Practical Considerations 11 IV. Advanced FP Applications 13 V. Future Directions 17 References 1 H 2. Functional Fusion Proteins by Random Transposon-Based GFP Insertion Robert Mealer, Heather Butler, and Tilomas Hughes I. Introduction 24 II. Rationale 27 III. Methods 31 IV. Materials 40 V. Discussion 42 References 43 3. Fluorescent Proteins tor Photoactivation Experiments Jennifer Lippiiiíútt-Schtrart: and George H. Patterson I. Why Use a Fluorescent Protein? 46 II. Why Use a Photoactivatable Fluorescent Protein? 46 III. Survey of Photoactivatable Fluorescent Proteins 47 IV. Uses of Photoactivatable Fluorescent Proteins 52 V. Future Directions ot Photoactivatable Fluorescent Proteins 58 References 59 Contents 4. Design and Optimization of Genetically Encoded Fluorescent Biosensors: GTPase Biosensors Louts Hodgson, Olivier Pertz, and Klaus M. Halm I. Introduction 64 II. Background: Factors Influencing FRET Efficiency 66 III. Design and Cloning of Biosensors 67 IV. Validation of the Biosensor in Cell Suspensions 69 V. Microscopy and Imaging Considerations 73 VI. Conclusion 75 VII. Appendix I 77 VIII. Appendix II 79 References 79 5. Fast 4D Microscopy J. R. De Mey, P. Kessler,]. Dompierre, F. P. Cordelières, A. Dietenen, J.-L. Vonesch, andJ.-B. Sibarita I. Introduction 84 Ii. Fast 4D Imaging: Definition, Interest, and Limits 87 III. Points to Consider Before Working with Fast 4D Imaging Systems 89 IV. Conclusions 107 References 110 6. Single-Molecule Imaging ot Fluorescent Proteins Adam D. Douglass and Rotiald D. I 'ale I. Introduction 114 II. Instrumentation 115 III. Fluorophores 118 IV. Reducing Protein Expression Levels 119 V. Biological Preparations 121 VI. Data Analysis and Interpretation 122 VII. Future Prospects 123 References 124 7. Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins Ajit P. Joglekar. E. D. Salinou, and Kerry S. Bloom I. Introduction 128 II. Counting Kinetochore Protein Numbers in Budding Yeast 130 III. Sample Preparation 134 Contents IV. Microscope and Image Acquisition System 135 V. Measurement of Flviorescence Signal 137 VI. Validation of Measurement Method 140 VII. Results 142 VIII. Discussion 144 IX. Conclusions 148 References 149 Fluorescent Protein Applications in Plants R. Howard Berg and Roger X. Beacliy I. Introduction 154 II. Expression and Function of FPs in Plants 155 III. Imaging 164 IV. Advanced Techniques 170 V. Summan' 173 References 174 9. Expression and Imaging of Fluorescent Proteins in the C elegans Gonad and Early Embryo Rebecca A. Green, Anjou Audhya, Andrei Po:niakovsky, Alexander Dammermann, Науку Penible, Joost Moneti, Xathan Portier, Anthony Hynian, Arshad Desai, and Karen Ocgema I. Introduction 180 II. Fluorescent Proteins in the C. elegans Gonad and Early Embryo 183 III. Transgene Expression in the C. elegans Germ Line: Breaking the Silence 187 ÌV. Constructing Fluorescent Worm Lines 188 V. Using Fluorescent Worm Strains 202 VI. Summan- 210 Appendix 211 References 213 10. Fluorescent Proteins in Zebrafish Cell and Developmental Biology H. William Derrick, III I. Introduction 220 II. Zebrafish Kinesin Genes in Early Development: A Cytokinetic Role for zMklp 1 221 III. Cell-Specific. Laser-Induced Transgene Expression in the Zebrafish Embryo: The ЅешаЗа I Gene in Axonal Guidance 226 IV. Transgeni c Zebrafish Models of Myc-Induced T-Cell Acute Lymphoblastic Leukemia 232 V. Summan' 236 References 237 Contents 11. Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain Juan Manuel Encinas and Grigori Enikolopov I. Introduction 244 II. Protocol I: Immimofluorescence Microscopy of Nestin-GFP and Nestin-CFPnuc Cells 254 III. Protocol II: The Use of Confocal Stereologţ' to Quantify Changes in Defined Classes of Neuronal Precursors 258 IV. Protocol III·. Electron Microscopy of Nestin-GFP/CFPnuc Cells 265 References 270 12. Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells Emmanuelle Querido and Pascal Chartrand I. Introduction 274 II. The MS2-GFP System 274 III. RNA Trafficking in Fibroblasts 278 IV. Following RNA Trafficking in Living Yeasts 287 References 291 13. Visualizing mRNA Localization and Local Protein Translation in Neurons Ralf Dahin, Manuel Zcitelhofer, Bernhard Götze, Michael A. Kiebler, and Paolo Macchi I. Introduction 294 II. Visualization of RNA Transport via RNA-Binding Proteins in Neurons 296 III. Visualization of RNP Transport 308 IV. Visualization of RNP Assembly and Composition 31 fi V. Visualization of Interactions Between RNAs and fra/w-Acting Factors 318 VI. Visualization of Local mRNA Translation 320 VII. Outlook 323 References 324 14. Quantitative FRAP in Analysis of Molecular Binding Dynamics in l 'ivo James G. McXally 1. Introduction 330 II. Rationale 330 HI. Methods 331 IV. Materials 348 V. Discussion 348 VI. Summan· 349 References 350 Contents 15. Quantitative and Qualitative Analysis of Plant Membrane Traffic Using Fluorescent Proteins Marketa Samalova, Mark Flicker, and ¡an Moon· I. Introduction 354 II. Rationale 362 III. Material 365 IV. Methods 365 V. Discussion 375 VI. Summary 378 References 378 16. Engineering FRET Constructs Using CFP and YFP Satosld Slmnozoiio and Atsushi Mtyaivaki I. Introduction 382 II. Rationale 382 III. Methods 384 References 393 17. Fluorescence Anisotropy Imaging Microscopy for Homo-FRET in Living Cells Marc Trainier and Malte Coppcy-Moisan I. Introduction 396 II. Photoselection Process 396 III. Rotational Depolarization 398 IV. Experimental Measurement of Fluorescence Anisotropy Decay in Confocal Microscopy 399 V. Fluorescence Anisotropy Decay of GFP-Tagged Proteins 402 VI. Fluorescence Depolarization by Homo-FRET 403 VII. Steady-State Fluorescence Anisotropy Imaging 406 VIII. Imaging Homo-FRET by Two-Photon FAIM 409 IX. Biological Applications with GFP-Tagged Proteins 410 X. Conclusion 413 References 413 18. FRET by Fluorescence Polarization Microscopy David И" Piste» and Mark A. Rizzo I. Introduction 416 II. Measuring FRET by Polarization Microscopy 418 III. Configuration of Microscopes for AFRET 419 IV. Calculation of Fluorescence Anisotropy 424 Contents V. Sample Preparation 426 VI. Conclusions 429 References 429 19. Bimolecular Fluorescence Complementation: Visualization of Molecular Interactions in Living Cells Tom K. Kirppola I. Introduction 433 II. Approaches for the Investigation of Protein Interactions 434 III. Bimolecular Fluorescence Complementation Analysis 439 IV. Experimental Strategies for BiFC Analysis 444 V. Examples of Protein Interactions That Have Been Visualized Using the BiFC Assay 449 VI. BiFC Analysis of Interactions in Different Organisms 454 VII. Screens Using the BiFC Approach 455 VIII. Analysis of Complex Dynamics Using the BiFC Approach 455 IX. Simultaneous Visualization of Several Protein Complexes 456 X. Experimental Strategies for Multicolor BiFC Analysis 458 XI. Limitations of the Multicolor BiFC Assay for Analysis of the Efficiencies of Protein Interactions in Cells 460 XII. Interaction Partners Whose Competition Has Been Visualized Using the Multicolor BiFC Assay 460 XIII. Visualization of Ubiquitin Family Peptide Conjugates in Cells 461 XIV. Ubiquitin Family Peptide Conjugates That Have Been Visualized Using the UbFC Assay 462 XV. Comparison of BiFC Analysis with Other Methods for the Visualization of Protein Interactions in Living Cells 463 XVI. Future Opportunities and Challenges 464 References 465 20. Protein—Protein Interactions Determined by Fluorescence Correlation Spectroscopy I. Introduction 472 II. FCS Theory 475 III. Two-Color Cross-Correlation 477 IV. Protein—Protein Interactions Using FCCS and Nongenetic Labels 47H V. Protein—Protein Interactions In Vivo Using FCCS and Autofluorescent Proteins 479 References 482 Contents 21. Recent Advances on In Vivo Imaging with Fluorescent Proteins Robert M. Hoffman I. Macroimaging with Fluorescent Proteins 486 II. Single-Cell In Vivo Imaging with Fluorescent Proteins 487 III. Imaging Dual-Color Angiogenesis and Tumors with Fluorescent Properties 488 IV. Imaging Tumor-Host Interaction with Fluorescent Proteins 489 V. New Applications for Fluorescent Proteins In Vivo: The Development of Effective Bacterial Therapy of Cancer 491 VI. Conclusions 492 References 493 22. Computational Processing and Analysis of Dynamic Fluorescence Image Data Jonas F. Dorn, Ganden: Dannser, and Ge Yang I. Introduction 498 II. Rationale 499 III. Image Features and Representation of Dynamic Events 502 IV. Methods 505 V. Two Case Studies of Image Analysis Applied to Mechanistic Cell Biology 520 VI. Performance Evaluation for Quality Control 527 VII. Summary 532 References 533 23. Automated Classification of Mitotic Phenotypes of Human Cells Using Fluorescent Proteins Л*. Harder, R. Eils, and K. Rohr I. Introduction 540 II. Segmentation of Multiceli Images 541 III. Extraction of Image Features 544 IV. Image Features 545 V. Classification ot Mitotic Patterns 547 VI. Experimental Results 548 VIII. Conclusion 552 References 553 24. Open Tools for Storage and Management ot Quantitative Image Data Joshua Moore, Chris Allan, Jean-Marie Burel, Brian Granger, Dotiald MacDonald, Jonathan Monk, and Jason R. Swedlow I. Introduction 556 II. Secure, Archived and Available Storage for Biological Image Data 557 Contents III. The Open Microscopy Environment: Data Management Tools for Biological Research 559 IV. The OMERO Server: A New Server Application for Data Management 561 V. Future Directions 569 References 570 Index 571 Volumes in Series 585
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publishDateSearch	2008
publishDateSort	2008
publisher	Elsevier AP
record_format	marc
series	Methods in cell biology
series2	Methods in cell biology
spelling	Fluorescent proteins ed. by Kevin F. Sullivan 2. ed. Amsterdam [u.a.] Elsevier AP 2008 XX, 592 S., [24] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 85 Auf der Rückseite des Titelblattes fälschlich als 1. ed. bezeichnet. - 1. Aufl. u.d.T.: Green fluorescent proteins Biofluorescence Fluorescent polymers Proteins Proteine (DE-588)4076388-2 gnd rswk-swf Fluoreszenz (DE-588)4154818-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Fluoreszenz (DE-588)4154818-8 s b DE-604 Sullivan, Kevin Francis 1955- Sonstige (DE-588)135581044 oth Methods in cell biology 85 (DE-604)BV002534878 85 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016275122&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Fluorescent proteins Methods in cell biology Biofluorescence Fluorescent polymers Proteins Proteine (DE-588)4076388-2 gnd Fluoreszenz (DE-588)4154818-8 gnd
subject_GND	(DE-588)4076388-2 (DE-588)4154818-8 (DE-588)4143413-4
title	Fluorescent proteins
title_auth	Fluorescent proteins
title_exact_search	Fluorescent proteins
title_exact_search_txtP	Fluorescent proteins
title_full	Fluorescent proteins ed. by Kevin F. Sullivan
title_fullStr	Fluorescent proteins ed. by Kevin F. Sullivan
title_full_unstemmed	Fluorescent proteins ed. by Kevin F. Sullivan
title_short	Fluorescent proteins
title_sort	fluorescent proteins
topic	Biofluorescence Fluorescent polymers Proteins Proteine (DE-588)4076388-2 gnd Fluoreszenz (DE-588)4154818-8 gnd
topic_facet	Biofluorescence Fluorescent polymers Proteins Proteine Fluoreszenz Aufsatzsammlung
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016275122&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV002534878
work_keys_str_mv	AT sullivankevinfrancis fluorescentproteins

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