Holdings: Microtubule protocols

Microtubule protocols:

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Bibliographic Details
Format:	Book
Language:	English
Published:	Totowa, NJ Humana Press 2007
Series:	Methods in molecular medicine 137
Subjects:	Microtubules Systèmes de libération d'un principe actif Tumeurs - traitement médicamenteux Microtubules > Research > Methodology Drug Delivery Systems Microtubules > drug effects Neoplasms > drug therapy Mikrotubulus Molekulare Medizin
Online Access:	Table of contents only Inhaltsverzeichnis Klappentext
Physical Description:	X, 305 S. Ill., graph. Darst.
ISBN:	1588296423 9781588296429

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adam_text	Contents Preface .............................................................................................................. v Contributors .....................................................................................................ix 1 Microtubules: An Overview Richard H. Wade.................................................................................. 1 2 Large Scale Purification of Brain Tubulin with the Modified Weisenberg Procedure fosé Manuel Andreu ............................................................................17 3 Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts Vincent Cache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko and Andrei V. Popov ......................................29 4 Preparation and Characterization of Post-Translationally Modified Tubulins from Anemia franciscana Paul A. O Connell and Thomas H. MacRae ........................................45 5 Studying the Structure of Microtubules by Electron Microscopy Linda A. Amos and Keiko Hirose ........................................................65 6 Fluorescence Microscopy oí Microtubules in Cultured Cells Irina Semenova and Vladimir Rodionov .............................................93 7 Measurements of Stathmin-Tubulin Interaction in Solution Marie-France Carlier ......................................................................... 703 8 Analysis of Microtubule Dynamics by Polarized Light Rudolf Oldenbourg ...........................................................................111 9 Visualization of Spindle Behavior Using Confocal Microscopy Adam I. Marcus. ................................................................................125 10 Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila Daniel W. Buster and Davidi. Sharp ............................................... 139 11 Analysis of Tubulin Transport in Nerve Processes Andrey Tsvetkov and Sergey Popov ..................................................161 12 Analysis of Microtubule-Mediated Intracellular Viral Transport Chunyong Liu, Min Liu and Jun Zhou.. ............................,................175 viii Contents 13 Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions Tong Zhang and Craig A. Mandato ...................................................181 14 Screening for Inhibitors of Microtubule-Associated Motor Proteins Frank Kozielski, Salvatore DeBonis and Dimitrios Skoufìas ............189 15 Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy Amelia B. Zelnak ...............................................................................209 16 Studying Drug-Tubulin Interactions by Х -Ray Crystallography Audrey Dorleans, Marcel Knossow and Benoît Gigant.................... 235 17 Characterizing Ligand-Microtubule Binding by Competition Methods José Fernando Diaz and Rubén Martínez Buey ................................245 18 Methods for Studying Vinca Alkaloid Interactions with Tubulin Sharon Lobért and John J. Correia ....................................................261 19 High-Throughput Screening of Microtubule-Interacting Drugs Susan L Bane, Rudravajhala Ravindra and Anna A. Zaydman ........281 20 Strategies for the Development of Novel Taxol-Like Agents Susan L Mooberry ............................................................................289 Index ............................................................................................................303 Microtubule Protocols Edited by Jun Zhou Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration, Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule pro¬ teins, analysis of post-translationai modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research. Includes methods for the development and discovery of novel agents that target microtubules Includes readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes Microtubules: An Overview. Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure. Purification and Mass Spectrometry Identification of Microtubule-Binding Proteins From XenopusEgg Extracts. Preparation andCharacterizafionofPosttrans- lationalfy Modified Tubulins From Anemia franciscana. Studying the Structure of Microtubules by Electron Microscopy. Fluorescence Microscopy of Microtubules in Cultured Cells. Measurements of Stathmin-Tubulin Interaction in Solution. Analysis of Microtubule Dynamics by Polarized Light. Visualization of Spindle Behavior Using Confocal Microscopy. Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila. Analysis of TubulinTnnsport in Nerve Processes. Analysis ofMicrotubuIe-Medi- ated Intracelluiar Viral Transport. Xenopus Oocyte Wound Healing as a Model System for Analysis of Micrombule-Actin Interactions. Screening for Inhibitors of Microtubule-Associated Motor Proteins. Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy. Studying Drug-Tubulin Interactions by X-Ray Crystallography. Characterizing Ligand-Microtubule Binding by Competition Methods. Methods for Studying Vinca Alkaloid Interactions With Tubulin. High-Throughput Screening of Micro¬ tubule-interacting Drugs. Strategies for the Development of Novel Taxol-Like Agents. Index.
adam_txt	Contents Preface . v Contributors .ix 1 Microtubules: An Overview Richard H. Wade. 1 2 Large Scale Purification of Brain Tubulin with the Modified Weisenberg Procedure fosé Manuel Andreu .17 3 Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts Vincent Cache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko and Andrei V. Popov .29 4 Preparation and Characterization of Post-Translationally Modified Tubulins from Anemia franciscana Paul A. O'Connell and Thomas H. MacRae .45 5 Studying the Structure of Microtubules by Electron Microscopy Linda A. Amos and Keiko Hirose .65 6 Fluorescence Microscopy oí Microtubules in Cultured Cells Irina Semenova and Vladimir Rodionov .93 7 Measurements of Stathmin-Tubulin Interaction in Solution Marie-France Carlier . 703 8 Analysis of Microtubule Dynamics by Polarized Light Rudolf Oldenbourg .111 9 Visualization of Spindle Behavior Using Confocal Microscopy Adam I. Marcus. .125 10 Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila Daniel W. Buster and Davidi. Sharp . 139 11 Analysis of Tubulin Transport in Nerve Processes Andrey Tsvetkov and Sergey Popov .161 12 Analysis of Microtubule-Mediated Intracellular Viral Transport Chunyong Liu, Min Liu and Jun Zhou. .,.175 viii Contents 13 Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions Tong Zhang and Craig A. Mandato .181 14 Screening for Inhibitors of Microtubule-Associated Motor Proteins Frank Kozielski, Salvatore DeBonis and Dimitrios Skoufìas .189 15 Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy Amelia B. Zelnak .209 16 Studying Drug-Tubulin Interactions by Х -Ray Crystallography Audrey Dorleans, Marcel Knossow and Benoît Gigant. 235 17 Characterizing Ligand-Microtubule Binding by Competition Methods José Fernando Diaz and Rubén Martínez Buey .245 18 Methods for Studying Vinca Alkaloid Interactions with Tubulin Sharon Lobért and John J. Correia .261 19 High-Throughput Screening of Microtubule-Interacting Drugs Susan L Bane, Rudravajhala Ravindra and Anna A. Zaydman .281 20 Strategies for the Development of Novel Taxol-Like Agents Susan L Mooberry .289 Index .303 Microtubule Protocols Edited by Jun Zhou Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration, Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule pro¬ teins, analysis of post-translationai modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research. Includes methods for the development and discovery of novel agents that target microtubules Includes readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes Microtubules: An Overview. Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure. Purification and Mass Spectrometry Identification of Microtubule-Binding Proteins From XenopusEgg Extracts. Preparation andCharacterizafionofPosttrans- lationalfy Modified Tubulins From Anemia franciscana. Studying the Structure of Microtubules by Electron Microscopy. Fluorescence Microscopy of Microtubules in Cultured Cells. Measurements of Stathmin-Tubulin Interaction in Solution. Analysis of Microtubule Dynamics by Polarized Light. Visualization of Spindle Behavior Using Confocal Microscopy. Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila. Analysis of TubulinTnnsport in Nerve Processes. Analysis ofMicrotubuIe-Medi- ated Intracelluiar Viral Transport. Xenopus Oocyte Wound Healing as a Model System for Analysis of Micrombule-Actin Interactions. Screening for Inhibitors of Microtubule-Associated Motor Proteins. Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy. Studying Drug-Tubulin Interactions by X-Ray Crystallography. Characterizing Ligand-Microtubule Binding by Competition Methods. Methods for Studying Vinca Alkaloid Interactions With Tubulin. High-Throughput Screening of Micro¬ tubule-interacting Drugs. Strategies for the Development of Novel Taxol-Like Agents. Index.
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spelling	Microtubule protocols ed. by Jun Zhou Totowa, NJ Humana Press 2007 X, 305 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 137 Microtubules ram Systèmes de libération d'un principe actif Tumeurs - traitement médicamenteux Microtubules Research Methodology Microtubules Drug Delivery Systems Microtubules drug effects Neoplasms drug therapy Mikrotubulus (DE-588)4169864-2 gnd rswk-swf Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Mikrotubulus (DE-588)4169864-2 s Molekulare Medizin (DE-588)4543844-4 s b DE-604 Zhou, Jun Sonstige oth Methods in molecular medicine 137 (DE-604)BV011334960 137 http://www.loc.gov/catdir/toc/ecip0619/2006027756.html Table of contents only Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016146013&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016146013&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext
spellingShingle	Microtubule protocols Methods in molecular medicine Microtubules ram Systèmes de libération d'un principe actif Tumeurs - traitement médicamenteux Microtubules Research Methodology Microtubules Drug Delivery Systems Microtubules drug effects Neoplasms drug therapy Mikrotubulus (DE-588)4169864-2 gnd Molekulare Medizin (DE-588)4543844-4 gnd
subject_GND	(DE-588)4169864-2 (DE-588)4543844-4
title	Microtubule protocols
title_auth	Microtubule protocols
title_exact_search	Microtubule protocols
title_exact_search_txtP	Microtubule protocols
title_full	Microtubule protocols ed. by Jun Zhou
title_fullStr	Microtubule protocols ed. by Jun Zhou
title_full_unstemmed	Microtubule protocols ed. by Jun Zhou
title_short	Microtubule protocols
title_sort	microtubule protocols
topic	Microtubules ram Systèmes de libération d'un principe actif Tumeurs - traitement médicamenteux Microtubules Research Methodology Microtubules Drug Delivery Systems Microtubules drug effects Neoplasms drug therapy Mikrotubulus (DE-588)4169864-2 gnd Molekulare Medizin (DE-588)4543844-4 gnd
topic_facet	Microtubules Systèmes de libération d'un principe actif Tumeurs - traitement médicamenteux Microtubules Research Methodology Drug Delivery Systems Microtubules drug effects Neoplasms drug therapy Mikrotubulus Molekulare Medizin
url	http://www.loc.gov/catdir/toc/ecip0619/2006027756.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016146013&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016146013&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA
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